Crumpling of silver nanowires by endolysosomes strongly reduces toxicity

Lehmann, Sylvia G.; Toybou, Djadidi; Pradas del Real, Ana-Elena; Arndt, Devrah; Tagmount, Abderrahmane; Viau, Muriel; Safi, Malak; Pacureanu, Alexandra; Cloetens, Peter; Bohic, Sylvain; Salomé, Murielle; Castillo-Michel, Hiram; Omaña-Sanz, Brenda; Hofmann, Annette; Vulpe, Christopher; Simonato, Jean-Pierre; Celle, Caroline; Charlet, Laurent; Gilbert, Benjamin

doi:10.1073/pnas.1820041116

Title: Crumpling of silver nanowires by endolysosomes strongly reduces toxicity

Journal Article · 08 July 2019 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.1820041116 · OSTI ID:1564566

Lehmann, Sylvia G. ^[1]; Toybou, Djadidi ^[1]; Pradas del Real, Ana-Elena ^[2]; Arndt, Devrah ^[3]; Tagmount, Abderrahmane ^[3]; Viau, Muriel ^[1]; Safi, Malak ^[1];

^[2];

^[4]; Salomé, Murielle ^[2]; Castillo-Michel, Hiram ^[2];

^[5]; Hofmann, Annette ^[5]; Vulpe, Christopher ^[3]; Simonato, Jean-Pierre ^[1]; Celle, Caroline ^[1]; Charlet, Laurent ^[1]; Gilbert, Benjamin ^[6]

Univ. of Grenoble (France)
European Synchrotron Radiation Facility (ESRF), Grenoble (France)
Univ. of Florida, Gainesville, FL (United States)
European Synchrotron Radiation Facility (ESRF), Grenoble (France); National Institute of Health and Medical Research (INSERM), Grenoble (France)
Univ. of Lille (France); Univ. of the Littoral Opal Coast, Wimereux (France)
Univ. of Grenoble (France); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Fibrous particles interact with cells and organisms in complex ways that can lead to cellular dysfunction, cell death, inflammation, and disease. The development of conductive transparent networks (CTNs) composed of metallic silver nanowires (AgNWs) for flexible touchscreen displays raises new possibilities for the intimate contact between novel fibers and human skin. Here, we report that a material property, nanowire-bending stiffness that is a function of diameter, controls the cytotoxicity of AgNWs to nonimmune cells from humans, mice, and fish without deterioration of critical CTN performance parameters: electrical conductivity and optical transparency. Both 30- and 90-nm-diameter AgNWs are readily internalized by cells, but thinner NWs are mechanically crumpled by the forces imposed during or after endocytosis, while thicker nanowires puncture the enclosing membrane and release silver ions and lysosomal contents to the cytoplasm, thereby initiating oxidative stress. This finding extends the fiber pathology paradigm and will enable the manufacture of safer products incorporating AgNWs.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1564566

Alternate ID(s):: OSTI ID: 1559232

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, Issue 30; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 25 works

Citation information provided by
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