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Title: SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility

Journal Article · · Journal of Cell Biology

The canonical Scribble polarity complex is implicated in regulation of epithelial junctions and apical polarity. Here, we show that SGEF, a RhoG-specific GEF, forms a ternary complex with Scribble and Dlg1, two members of the Scribble complex. SGEF targets to apical junctions in a Scribble-dependent fashion and functions in the regulation of actomyosin-based contractility and barrier function at tight junctions as well as E-cadherin–mediated formation of adherens junctions. Surprisingly, SGEF does not control the establishment of polarity. However, in 3D cysts, SGEF regulates the formation of a single open lumen. Interestingly, SGEF’s nucleotide exchange activity regulates the formation and maintenance of adherens junctions, and in cysts the number of lumens formed, whereas SGEF’s scaffolding activity is critical for regulation of actomyosin contractility and lumen opening. We propose that SGEF plays a key role in coordinating junctional assembly and actomyosin contractility by bringing together Scribble and Dlg1 and targeting RhoG activation to cell–cell junctions.

Sponsoring Organization:
USDOE
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1564283
Alternate ID(s):
OSTI ID: 1625176
Journal Information:
Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 8 Vol. 218; ISSN 0021-9525
Publisher:
Rockefeller University PressCopyright Statement
Country of Publication:
United States
Language:
English

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  • Penkert, Rhiannon R.; DiVittorio, Heather M.; Prehoda, Kenneth E.
  • Nature Structural & Molecular Biology, Vol. 11, Issue 11 https://doi.org/10.1038/nsmb839
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