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Title: SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility

Abstract

The canonical Scribble polarity complex is implicated in regulation of epithelial junctions and apical polarity. Here, we show that SGEF, a RhoG-specific GEF, forms a ternary complex with Scribble and Dlg1, two members of the Scribble complex. SGEF targets to apical junctions in a Scribble-dependent fashion and functions in the regulation of actomyosin-based contractility and barrier function at tight junctions as well as E-cadherin–mediated formation of adherens junctions. Surprisingly, SGEF does not control the establishment of polarity. However, in 3D cysts, SGEF regulates the formation of a single open lumen. Interestingly, SGEF’s nucleotide exchange activity regulates the formation and maintenance of adherens junctions, and in cysts the number of lumens formed, whereas SGEF’s scaffolding activity is critical for regulation of actomyosin contractility and lumen opening. We propose that SGEF plays a key role in coordinating junctional assembly and actomyosin contractility by bringing together Scribble and Dlg1 and targeting RhoG activation to cell–cell junctions.

Authors:
ORCiD logo [1];  [2];  [2];  [1]; ORCiD logo [3]; ORCiD logo [3]; ORCiD logo [1];  [4];  [4]; ORCiD logo [3]; ORCiD logo [2]; ORCiD logo [1]
  1. Department of Biological Sciences, The University of Toledo, Toledo, OH
  2. Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI
  3. Department of Biochemistry, University of Iowa, Iowa City, IA
  4. International Center for Genetic Engineering and Biotechnology, Trieste, Italy
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source; Univ. of Toledo, OH (United States); Univ. of Michigan, Ann Arbor, MI (United States); Univ. of Iowa, Iowa City, IA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Inst. of Health (NIH) (United States); Associazione Italiana per la Ricerca sul Cancro (Italy); American Heart Association (United States)
OSTI Identifier:
1564283
Alternate Identifier(s):
OSTI ID: 1625176
Grant/Contract Number:  
AC02-05CH11231; 1R15CA199101; 1R21CA194776; 1R03CA197227; R01 GM112794; 18578; 15GRNT25740021
Resource Type:
Published Article
Journal Name:
Journal of Cell Biology
Additional Journal Information:
Journal Name: Journal of Cell Biology Journal Volume: 218 Journal Issue: 8; Journal ID: ISSN 0021-9525
Publisher:
Rockefeller University Press
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; adhesion; biochemistry; polarity

Citation Formats

Awadia, Sahezeel, Huq, Farah, Arnold, Torey R., Goicoechea, Silvia M., Sun, Young Joo, Hou, Titus, Kreider-Letterman, Gabriel, Massimi, Paola, Banks, Lawrence, Fuentes, Ernesto J., Miller, Ann L., and Garcia-Mata, Rafael. SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. United States: N. p., 2019. Web. doi:10.1083/jcb.201811114.
Awadia, Sahezeel, Huq, Farah, Arnold, Torey R., Goicoechea, Silvia M., Sun, Young Joo, Hou, Titus, Kreider-Letterman, Gabriel, Massimi, Paola, Banks, Lawrence, Fuentes, Ernesto J., Miller, Ann L., & Garcia-Mata, Rafael. SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. United States. doi:10.1083/jcb.201811114.
Awadia, Sahezeel, Huq, Farah, Arnold, Torey R., Goicoechea, Silvia M., Sun, Young Joo, Hou, Titus, Kreider-Letterman, Gabriel, Massimi, Paola, Banks, Lawrence, Fuentes, Ernesto J., Miller, Ann L., and Garcia-Mata, Rafael. Thu . "SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility". United States. doi:10.1083/jcb.201811114.
@article{osti_1564283,
title = {SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility},
author = {Awadia, Sahezeel and Huq, Farah and Arnold, Torey R. and Goicoechea, Silvia M. and Sun, Young Joo and Hou, Titus and Kreider-Letterman, Gabriel and Massimi, Paola and Banks, Lawrence and Fuentes, Ernesto J. and Miller, Ann L. and Garcia-Mata, Rafael},
abstractNote = {The canonical Scribble polarity complex is implicated in regulation of epithelial junctions and apical polarity. Here, we show that SGEF, a RhoG-specific GEF, forms a ternary complex with Scribble and Dlg1, two members of the Scribble complex. SGEF targets to apical junctions in a Scribble-dependent fashion and functions in the regulation of actomyosin-based contractility and barrier function at tight junctions as well as E-cadherin–mediated formation of adherens junctions. Surprisingly, SGEF does not control the establishment of polarity. However, in 3D cysts, SGEF regulates the formation of a single open lumen. Interestingly, SGEF’s nucleotide exchange activity regulates the formation and maintenance of adherens junctions, and in cysts the number of lumens formed, whereas SGEF’s scaffolding activity is critical for regulation of actomyosin contractility and lumen opening. We propose that SGEF plays a key role in coordinating junctional assembly and actomyosin contractility by bringing together Scribble and Dlg1 and targeting RhoG activation to cell–cell junctions.},
doi = {10.1083/jcb.201811114},
journal = {Journal of Cell Biology},
number = 8,
volume = 218,
place = {United States},
year = {2019},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.1083/jcb.201811114

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Works referenced in this record:

Regulation of circular dorsal ruffles, macropinocytosis, and cell migration by RhoG and its exchange factor, Trio
journal, July 2017

  • Valdivia, Alejandra; Goicoechea, Silvia M.; Awadia, Sahezeel
  • Molecular Biology of the Cell, Vol. 28, Issue 13
  • DOI: 10.1091/mbc.e16-06-0412

Binding of GEF-H1 to the Tight Junction-Associated Adaptor Cingulin Results in Inhibition of Rho Signaling and G1/S Phase Transition
journal, May 2005


Interaction of p190RhoGAP with C-terminal Domain of p120-catenin Modulates Endothelial Cytoskeleton and Permeability
journal, May 2013

  • Zebda, Noureddine; Tian, Yufeng; Tian, Xinyong
  • Journal of Biological Chemistry, Vol. 288, Issue 25
  • DOI: 10.1074/jbc.M112.432757

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

  • Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
  • DOI: 10.1107/S0907444996012255

Distinct roles of cadherin-6 and E-cadherin in tubulogenesis and lumen formation
journal, June 2011

  • Jia, Liwei; Liu, Fengming; Hansen, Steen H.
  • Molecular Biology of the Cell, Vol. 22, Issue 12
  • DOI: 10.1091/mbc.e11-01-0038

ZO-1 interactions with F-actin and occludin direct epithelial polarization and single lumen specification in 3D culture
journal, October 2016

  • Odenwald, Matthew A.; Choi, Wangsun; Buckley, Aaron
  • Journal of Cell Science, Vol. 130, Issue 1
  • DOI: 10.1242/jcs.188185

Centralspindlin and α-catenin regulate Rho signalling at the epithelial zonula adherens
journal, July 2012

  • Ratheesh, Aparna; Gomez, Guillermo A.; Priya, Rashmi
  • Nature Cell Biology, Vol. 14, Issue 8
  • DOI: 10.1038/ncb2532

Nectin and afadin: novel organizers of intercellular junctions
journal, January 2003


RhoG Signals in Parallel with Rac1 and Cdc42
journal, October 2002

  • Wennerberg, Krister; Ellerbroek, Shawn M.; Liu, Rong-Yu
  • Journal of Biological Chemistry, Vol. 277, Issue 49
  • DOI: 10.1074/jbc.M203816200

The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin
journal, December 2005

  • Qin, Yi; Capaldo, Christopher; Gumbiner, Barry M.
  • The Journal of Cell Biology, Vol. 171, Issue 6
  • DOI: 10.1083/jcb.200506094

Establishment of epithelial polarity – GEF who's minding the GAP?
journal, July 2014

  • Ngok, Siu P.; Lin, Wan-Hsin; Anastasiadis, Panos Z.
  • Journal of Cell Science, Vol. 127, Issue 15
  • DOI: 10.1242/jcs.153197

ZO-1 Knockout by TALEN-Mediated Gene Targeting in MDCK Cells: Involvement of ZO-1 in the Regulation of Cytoskeleton and Cell Shape
journal, August 2014


Depletion of E-Cadherin Disrupts Establishment but Not Maintenance of Cell Junctions in Madin-Darby Canine Kidney Epithelial Cells
journal, January 2007

  • Capaldo, Christopher T.; Macara, Ian G.
  • Molecular Biology of the Cell, Vol. 18, Issue 1
  • DOI: 10.1091/mbc.e06-05-0471

Origins of Allostery and Evolvability in Proteins: A Case Study
journal, July 2016


GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors
journal, February 2005

  • Rossman, Kent L.; Der, Channing J.; Sondek, John
  • Nature Reviews Molecular Cell Biology, Vol. 6, Issue 2
  • DOI: 10.1038/nrm1587

Direct Binding of the PDZ Domain of Dishevelled to a Conserved Internal Sequence in the C-Terminal Region of Frizzled
journal, November 2003


SGEF, a RhoG Guanine Nucleotide Exchange Factor that Stimulates Macropinocytosis
journal, July 2004

  • Ellerbroek, Shawn M.; Wennerberg, Krister; Arthur, William T.
  • Molecular Biology of the Cell, Vol. 15, Issue 7
  • DOI: 10.1091/mbc.e04-02-0146

Scribble is required for normal epithelial cell–cell contacts and lumen morphogenesis in the mammalian lung
journal, January 2013


Recognition of Unique Carboxyl-Terminal Motifs by Distinct PDZ Domains
journal, January 1997


Cell-cell adhesion interface: rise of the lateral membrane
journal, January 2017


Scribble: A master scaffold in polarity, adhesion, synaptogenesis, and proliferation
journal, December 2018

  • Bonello, Teresa T.; Peifer, Mark
  • The Journal of Cell Biology, Vol. 218, Issue 3
  • DOI: 10.1083/jcb.201810103

Recruitment of Scribble to the Synaptic Scaffolding Complex Requires GUK-holder, a Novel DLG Binding Protein
journal, April 2002


XDS
journal, January 2010

  • Kabsch, Wolfgang
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
  • DOI: 10.1107/S0907444909047337

Supracellular actomyosin assemblies during development
journal, March 2013


Endogenous RhoG Is Rapidly Activated after Epidermal Growth Factor Stimulation through Multiple Guanine-Nucleotide Exchange Factors
journal, May 2010

  • Samson, Thomas; Welch, Christopher; Monaghan-Benson, Elizabeth
  • Molecular Biology of the Cell, Vol. 21, Issue 9
  • DOI: 10.1091/mbc.e09-09-0809

Localization of apical epithelial determinants by the basolateral PDZ protein Scribble
journal, February 2000

  • Bilder, David; Perrimon, Norbert
  • Nature, Vol. 403, Issue 6770
  • DOI: 10.1038/35001108

Rac1 orientates epithelial apical polarity through effects on basolateral laminin assembly
journal, August 2001

  • O'Brien, Lucy Erin; Jou, Tzuu-Shuh; Pollack, Anne L.
  • Nature Cell Biology, Vol. 3, Issue 9
  • DOI: 10.1038/ncb0901-831

Organization and execution of the epithelial polarity programme
journal, March 2014

  • Rodriguez-Boulan, Enrique; Macara, Ian G.
  • Nature Reviews Molecular Cell Biology, Vol. 15, Issue 4
  • DOI: 10.1038/nrm3775

Junctional Complexes in Various Epithelia
journal, May 1963


Comprehensive analysis of formin localization in Xenopus epithelial cells
journal, January 2019

  • Higashi, Tomohito; Stephenson, Rachel E.; Miller, Ann L.
  • Molecular Biology of the Cell, Vol. 30, Issue 1
  • DOI: 10.1091/mbc.E18-02-0133

ARHGAP10 is necessary for α-catenin recruitment at adherens junctions and for Listeria invasion
journal, September 2005

  • Sousa, Sandra; Cabanes, Didier; Archambaud, Cristel
  • Nature Cell Biology, Vol. 7, Issue 10
  • DOI: 10.1038/ncb1308

hScrib interacts with ZO-2 at the cell-cell junctions of epithelial cells
journal, June 2005


Structural basis for the differential interaction of Scribble PDZ domains with the guanine nucleotide exchange factor β-PIX
journal, October 2017

  • Lim, Krystle Y. B.; Gödde, Nathan J.; Humbert, Patrick O.
  • Journal of Biological Chemistry, Vol. 292, Issue 50
  • DOI: 10.1074/jbc.M117.799452

DLC1 Interaction with  -Catenin Stabilizes Adherens Junctions and Enhances DLC1 Antioncogenic Activity
journal, April 2012

  • Tripathi, V.; Popescu, N. C.; Zimonjic, D. B.
  • Molecular and Cellular Biology, Vol. 32, Issue 11
  • DOI: 10.1128/MCB.06580-11

Interaction between the internal motif KTXXXI of Idax and mDvl PDZ domain
journal, September 2004

  • London, Timothy B. C.; Lee, Ho-Jin; Shao, Youming
  • Biochemical and Biophysical Research Communications, Vol. 322, Issue 1
  • DOI: 10.1016/j.bbrc.2004.07.113

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Synaptic signaling by nitric oxide
journal, June 1997


The Invasive Capacity of HPV Transformed Cells Requires the hDlg-Dependent Enhancement of SGEF/RhoG Activity
journal, February 2012


Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7
journal, June 2008

  • Langer, Gerrit; Cohen, Serge X.; Lamzin, Victor S.
  • Nature Protocols, Vol. 3, Issue 7
  • DOI: 10.1038/nprot.2008.91

Free R value: a novel statistical quantity for assessing the accuracy of crystal structures
journal, January 1992


Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network
journal, October 2005

  • Hildebrand, J. D.
  • Journal of Cell Science, Vol. 118, Issue 22
  • DOI: 10.1242/jcs.02626

Interdomain interface-mediated target recognition by the Scribble PDZ34 supramodule
journal, May 2015

  • Ren, Jinqi; Feng, Lei; Bai, Yujie
  • Biochemical Journal, Vol. 468, Issue 1
  • DOI: 10.1042/BJ20141473

Maintenance of the Epithelial Barrier and Remodeling of Cell-Cell Junctions during Cytokinesis
journal, July 2016

  • Higashi, Tomohito; Arnold, Torey R.; Stephenson, Rachel E.
  • Current Biology, Vol. 26, Issue 14
  • DOI: 10.1016/j.cub.2016.05.036

Basolateral targeting by leucine-rich repeat domains in epithelial cells
journal, October 2003


The PDB_REDO server for macromolecular structure model optimization
journal, May 2014


E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions
journal, March 2005


The polarity protein Scribble positions DLC3 at adherens junctions to regulate Rho signaling
journal, August 2016

  • Hendrick, Janina; Franz-Wachtel, Mirita; Moeller, Yvonne
  • Journal of Cell Science, Vol. 129, Issue 19
  • DOI: 10.1242/jcs.190074

Tumor Suppressor Scribble Regulates Assembly of Tight Junctions in the Intestinal Epithelium
journal, January 2010

  • Ivanov, Andrei I.; Young, Cheryl; Beste, Kyle Den
  • The American Journal of Pathology, Vol. 176, Issue 1
  • DOI: 10.2353/ajpath.2010.090220

Anillin Regulates Cell-Cell Junction Integrity by Organizing Junctional Accumulation of Rho-GTP and Actomyosin
journal, June 2014


Differential Regulation of Endogenous Cadherin Expression in Madin-Darby Canine Kidney Cells by Cell-Cell Adhesion and Activation of β-Catenin Signaling
journal, March 2000

  • Stewart, Daniel B.; Barth, Angela I. M.; Nelson, W. James
  • Journal of Biological Chemistry, Vol. 275, Issue 27
  • DOI: 10.1074/jbc.M000467200

Rho GEFs in endothelial junctions: Effector selectivity and signaling integration determine junctional response
journal, December 2013

  • Ngok, Siu P.; Anastasiadis, Panos Z.
  • Tissue Barriers, Vol. 1, Issue 5
  • DOI: 10.4161/tisb.27132

α-Catenin as a tension transducer that induces adherens junction development
journal, May 2010

  • Yonemura, Shigenobu; Wada, Yuko; Watanabe, Toshiyuki
  • Nature Cell Biology, Vol. 12, Issue 6
  • DOI: 10.1038/ncb2055

RhoG GTPase Controls a Pathway That Independently Activates Rac1 and Cdc42Hs
journal, June 1998

  • Gauthier-Rouvière, Cécile; Vignal, Emmanuel; Mériane, Mayya
  • Molecular Biology of the Cell, Vol. 9, Issue 6
  • DOI: 10.1091/mbc.9.6.1379

ADP-Ribosylation Factor/COPI-dependent Events at the Endoplasmic Reticulum-Golgi Interface Are Regulated by the Guanine Nucleotide Exchange Factor GBF1
journal, June 2003

  • García-Mata, Rafael; Szul, Tomasz; Alvarez, Cecilia
  • Molecular Biology of the Cell, Vol. 14, Issue 6
  • DOI: 10.1091/mbc.e02-11-0730

The minimal cadherin-catenin complex binds to actin filaments under force
journal, October 2014


RhoG activates Rac1 by direct interaction with the Dock180-binding protein Elmo
journal, July 2003


Internal recognition through PDZ domain plasticity in the Par-6–Pals1 complex
journal, October 2004

  • Penkert, Rhiannon R.; DiVittorio, Heather M.; Prehoda, Kenneth E.
  • Nature Structural & Molecular Biology, Vol. 11, Issue 11
  • DOI: 10.1038/nsmb839

Morpholinos: Antisense and Sensibility
journal, October 2015


An ELMO2-RhoG-ILK network modulates microtubule dynamics
journal, July 2015

  • Jackson, Bradley C.; Ivanova, Iordanka A.; Dagnino, Lina
  • Molecular Biology of the Cell, Vol. 26, Issue 14
  • DOI: 10.1091/mbc.E14-10-1444

The Structure of the Tiam1 PDZ Domain/ Phospho-Syndecan1 Complex Reveals a Ligand Conformation that Modulates Protein Dynamics
journal, March 2013


Tight Junction Pore and Leak Pathways: A Dynamic Duo
journal, March 2011


SHIP2 regulates epithelial cell polarity through its lipid product, which binds to Dlg1, a pathway subverted by hepatitis C virus core protein
journal, July 2013

  • Awad, Aline; Sar, Sokhavuth; Barré, Ronan
  • Molecular Biology of the Cell, Vol. 24, Issue 14
  • DOI: 10.1091/mbc.e12-08-0626

Planar polarity pathway and Nance-Horan syndrome-like 1b have essential cell-autonomous functions in neuronal migration
journal, June 2011

  • Walsh, G. S.; Grant, P. K.; Morgan, J. A.
  • Development, Vol. 138, Issue 14
  • DOI: 10.1242/dev.063842

The Scribble–Dlg–Lgl polarity module in development and cancer: from flies to man
journal, August 2012

  • Elsum, Imogen; Yates, Laura; Humbert, Patrick O.
  • Essays in Biochemistry, Vol. 53
  • DOI: 10.1042/bse0530141

Cell Boundary Elongation by Non-autonomous Contractility in Cell Oscillation
journal, September 2016


MgcRacGAP restricts active RhoA at the cytokinetic furrow and both RhoA and Rac1 at cell–cell junctions in epithelial cells
journal, July 2015

  • Breznau, Elaina B.; Semack, Ansley C.; Higashi, Tomohito
  • Molecular Biology of the Cell, Vol. 26, Issue 13
  • DOI: 10.1091/mbc.E14-11-1553

Planar Cell Polarity Links Axes of Spatial Dynamics in Neural-Tube Closure
journal, May 2012


The Scribble Polarity Protein Stabilizes E-Cadherin/p120-Catenin Binding and Blocks Retrieval of E-Cadherin to the Golgi
journal, November 2012


Activation of Rac1 by RhoG regulates cell migration
journal, January 2006


Tools for live imaging of active Rho GTPases in Xenopus : STEPHENSON and MILLER
journal, January 2017

  • Stephenson, Rachel E.; Miller, Ann L.
  • genesis, Vol. 55, Issue 1-2
  • DOI: 10.1002/dvg.22998

Integration, scaling, space-group assignment and post-refinement
journal, January 2010

  • Kabsch, Wolfgang
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 133-144
  • DOI: 10.1107/S0907444909047374

Crosstalk between small GTPases and polarity proteins in cell polarization
journal, November 2008

  • Iden, Sandra; Collard, John G.
  • Nature Reviews Molecular Cell Biology, Vol. 9, Issue 11
  • DOI: 10.1038/nrm2521

Differential activation and function of Rho GTPases during Salmonella– host cell interactions
journal, October 2006

  • Patel, Jayesh C.; Galán, Jorge E.
  • The Journal of Cell Biology, Vol. 175, Issue 3
  • DOI: 10.1083/jcb.200605144

The interdependence of the Rho GTPases and apicobasal cell polarity
journal, April 2014


Integrated activity of PDZ protein complexes regulates epithelial polarity
journal, December 2002

  • Bilder, David; Schober, Markus; Perrimon, Norbert
  • Nature Cell Biology, Vol. 5, Issue 1
  • DOI: 10.1038/ncb897

Molecular mechanisms of de novo lumen formation
journal, September 2014

  • Sigurbjörnsdóttir, Sara; Mathew, Renjith; Leptin, Maria
  • Nature Reviews Molecular Cell Biology, Vol. 15, Issue 10
  • DOI: 10.1038/nrm3871

Tuba, a Cdc42 GEF, is required for polarized spindle orientation during epithelial cyst formation
journal, May 2010

  • Qin, Yi; Meisen, Walter H.; Hao, Yi
  • The Journal of Cell Biology, Vol. 189, Issue 4
  • DOI: 10.1083/jcb.201002097

Zonula occludens-1 and -2 regulate apical cell structure and the zonula adherens cytoskeleton in polarized epithelia
journal, February 2012

  • Fanning, Alan S.; Van Itallie, Christina M.; Anderson, James M.
  • Molecular Biology of the Cell, Vol. 23, Issue 4
  • DOI: 10.1091/mbc.e11-09-0791

Current knowledge of the large RhoGAP family of proteins
journal, February 2007

  • Tcherkezian, Joseph; Lamarche-Vane, Nathalie
  • Biology of the Cell, Vol. 99, Issue 2
  • DOI: 10.1042/BC20060086

Catching a GEF by its tail
journal, January 2007


The basics of epithelial-mesenchymal transition
journal, June 2009

  • Kalluri, Raghu; Weinberg, Robert A.
  • Journal of Clinical Investigation, Vol. 119, Issue 6
  • DOI: 10.1172/JCI39104

ZO-1 and ZO-2 Independently Determine Where Claudins Are Polymerized in Tight-Junction Strand Formation
journal, August 2006


Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster.
journal, June 1985


Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis
journal, January 2011

  • Terry, Stephen J.; Zihni, Ceniz; Elbediwy, Ahmed
  • Nature Cell Biology, Vol. 13, Issue 2
  • DOI: 10.1038/ncb2156

Drosophila melanogaster Guk-holder interacts with the Scribbled PDZ1 domain and regulates epithelial development with Scribbled and Discs Large
journal, January 2018

  • Caria, Sofia; Magtoto, Charlene M.; Samiei, Tinaz
  • Journal of Biological Chemistry, Vol. 293, Issue 12
  • DOI: 10.1074/jbc.M117.817528

Conditional targeting of E-cadherin in skin: Insights into hyperproliferative and degenerative responses
journal, January 2004

  • Tinkle, C. L.; Lechler, T.; Pasolli, H. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 2
  • DOI: 10.1073/pnas.0307437100

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

Polarized monolayers formed by epithelial cells on a permeable and translucent support
journal, June 1978


Physiology and Function of the Tight Junction
journal, August 2009


The RhoGAP protein Deleted in Liver Cancer 3 (DLC3) is essential for adherens junctions integrity
journal, June 2012


Junctional recruitment of mammalian Scribble relies on E-cadherin engagement
journal, April 2005

  • Navarro, Christel; Nola, Sébastien; Audebert, Stéphane
  • Oncogene, Vol. 24, Issue 27
  • DOI: 10.1038/sj.onc.1208632

Human Homolog of Disc-large Is Required for Adherens Junction Assembly and Differentiation of Human Intestinal Epithelial Cells
journal, December 2003

  • Laprise, Patrick; Viel, Alain; Rivard, Nathalie
  • Journal of Biological Chemistry, Vol. 279, Issue 11
  • DOI: 10.1074/jbc.M309843200

Nance–Horan syndrome protein, NHS, associates with epithelial cell junctions
journal, May 2006

  • Sharma, Shiwani; Ang, Sharyn L.; Shaw, Marie
  • Human Molecular Genetics, Vol. 15, Issue 12
  • DOI: 10.1093/hmg/ddl120

Unconventional Rac-GEF activity is mediated through the Dock180–ELMO complex
journal, July 2002

  • Brugnera, Enrico; Haney, Lisa; Grimsley, Cynthia
  • Nature Cell Biology, Vol. 4, Issue 8
  • DOI: 10.1038/ncb824

ZO-1 Stabilizes the Tight Junction Solute Barrier through Coupling to the Perijunctional Cytoskeleton
journal, September 2009

  • Van Itallie, Christina M.; Fanning, Alan S.; Bridges, Arlene
  • Molecular Biology of the Cell, Vol. 20, Issue 17
  • DOI: 10.1091/mbc.e09-04-0320

Remodeling the zonula adherens in response to tension and the role of afadin in this response
journal, April 2016

  • Choi, Wangsun; Acharya, Bipul R.; Peyret, Grégoire
  • The Journal of Cell Biology, Vol. 213, Issue 2
  • DOI: 10.1083/jcb.201506115

Small GTPase RhoG Is a Key Regulator for Neurite Outgrowth in PC12 Cells
journal, October 2000


RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration
journal, September 2007

  • van Buul, Jaap D.; Allingham, Michael J.; Samson, Thomas
  • The Journal of Cell Biology, Vol. 178, Issue 7
  • DOI: 10.1083/jcb.200612053

Actomyosin networks and tissue morphogenesis
journal, April 2014


Mammalian Scribble Forms a Tight Complex with the βPIX Exchange Factor
journal, June 2004


Developmental capacities of benign and malignant neoplasms ofDrosophila
journal, January 1974

  • Gateff, E.; Schneiderman, Howard A.
  • Wilhelm Roux Archiv f�r Entwicklungsmechanik der Organismen, Vol. 176, Issue 1
  • DOI: 10.1007/BF00577830

Building epithelial architecture: insights from three-dimensional culture models
journal, July 2002

  • O'Brien, Lucy Erin; Zegers, Mirjam M. P.; Mostov, Keith E.
  • Nature Reviews Molecular Cell Biology, Vol. 3, Issue 7
  • DOI: 10.1038/nrm859

The Nuclear RhoA Exchange Factor Net1 Interacts with Proteins of the Dlg Family, Affects Their Localization, and Influences Their Tumor Suppressor Activity
journal, October 2007

  • Garcia-Mata, R.; Dubash, A. D.; Sharek, L.
  • Molecular and Cellular Biology, Vol. 27, Issue 24
  • DOI: 10.1128/MCB.00157-07

hScrib is a functional homologue of the Drosophila tumour suppressor Scribble
journal, December 2003


Phaser crystallographic software
journal, July 2007

  • McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Journal of Applied Crystallography, Vol. 40, Issue 4
  • DOI: 10.1107/S0021889807021206

An Elmo–Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion
journal, December 2014

  • Toret, Christopher P.; Collins, Caitlin; Nelson, W. James
  • The Journal of Cell Biology, Vol. 207, Issue 5
  • DOI: 10.1083/jcb.201406135