Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”

Lewis, Brianne E.; Mason, Zachary; Rodrigues, Andria V.; Nuth, Manunya; Dizin, Eric; Cowan, J. A.; Stemmler, Timothy L.

doi:10.1039/C9MT00172G

Title: Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”

Abstract

Model for Zn association to the active site of yeast Fe–S cluster assembly scaffold protein Isu1.

Authors:

Lewis, Brianne E. ^[1]; Mason, Zachary ^[1]; Rodrigues, Andria V. ^[1]; Nuth, Manunya ^[2]; Dizin, Eric ^[2]; Cowan, J. A. ^[2];

^[1]

Department of Pharmaceutical Science, Wayne State University, Detroit, USA
Department of Chemistry, The Ohio State University, Columbus, USA

Publication Date:: 2019-11-13

Sponsoring Org.:: USDOE

OSTI Identifier:: 1562561

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Metallomics

Additional Journal Information:: Journal Name: Metallomics Journal Volume: 11 Journal Issue: 11; Journal ID: ISSN 1756-5901

Publisher:: Oxford University Press

Country of Publication:: United Kingdom

Language:: English

Citation Formats


                    Lewis, Brianne E., Mason, Zachary, Rodrigues, Andria V., Nuth, Manunya, Dizin, Eric, Cowan, J. A., and Stemmler, Timothy L. Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”.  United Kingdom: N. p., 2019. 
Web.  doi:10.1039/C9MT00172G.

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                    Lewis, Brianne E., Mason, Zachary, Rodrigues, Andria V., Nuth, Manunya, Dizin, Eric, Cowan, J. A., & Stemmler, Timothy L. Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”.  United Kingdom.  https://doi.org/10.1039/C9MT00172G

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                    Lewis, Brianne E., Mason, Zachary, Rodrigues, Andria V., Nuth, Manunya, Dizin, Eric, Cowan, J. A., and Stemmler, Timothy L. Wed .  
"Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”".  United Kingdom.  https://doi.org/10.1039/C9MT00172G.

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@article{osti_1562561,

  title        = {Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”},

  author       = {Lewis, Brianne E. and Mason, Zachary and Rodrigues, Andria V. and Nuth, Manunya and Dizin, Eric and Cowan, J. A. and Stemmler, Timothy L.},

  abstractNote = {Model for Zn association to the active site of yeast Fe–S cluster assembly scaffold protein Isu1.},

  doi          = {10.1039/C9MT00172G},

  journal      = {Metallomics},

  number       = 11,

  volume       = 11,

  place        = {United Kingdom},

  year         = {2019},

  month        = {11}

}

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Works referenced in this record:

Clustal Omega for making accurate alignments of many protein sequences: Clustal Omega for Many Protein Sequences
journal, October 2017

Sievers, Fabian; Higgins, Desmond G.
Protein Science, Vol. 27, Issue 1
DOI: 10.1002/pro.3290

Acidic residues of yeast frataxin have an essential role in Fe–S cluster assembly
journal, December 2006

Foury, Françoise; Pastore, Annalisa; Trincal, Mathieu
EMBO reports, Vol. 8, Issue 2
DOI: 10.1038/sj.embor.7400881

In vitro characterization of a novel Isu homologue from Drosophila melanogaster for de novo FeS-cluster formation
journal, January 2017

Dzul, Stephen P.; Rocha, Agostinho G.; Rawat, Swati
Metallomics, Vol. 9, Issue 1
DOI: 10.1039/C6MT00163G

Structure and Ubiquitin Interactions of the Conserved Zinc Finger Domain of Npl4
journal, March 2003

Wang, Bin; Alam, Steven L.; Meyer, Hemmo H.
Journal of Biological Chemistry, Vol. 278, Issue 22
DOI: 10.1074/jbc.M300459200

Human Frataxin Is an Allosteric Switch That Activates the Fe−S Cluster Biosynthetic Complex
journal, November 2010

Tsai, Chi-Lin; Barondeau, David P.
Biochemistry, Vol. 49, Issue 43
DOI: 10.1021/bi1013062

Regulation of Mitochondrial Iron Accumulation by Yfh1p, a Putative Homolog of Frataxin
journal, June 1997

Babcock, M.
Science, Vol. 276, Issue 5319
DOI: 10.1126/science.276.5319.1709

Progress and challenges in the theory and interpretation of X-ray spectra
journal, March 2001

Rehr, J. J.; Ankudinov, A. L.
Journal of Synchrotron Radiation, Vol. 8, Issue 2
DOI: 10.1107/S0909049500016423

Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism
journal, May 2019

Fox, Nicholas G.; Yu, Xiaodi; Feng, Xidong
Nature Communications, Vol. 10, Issue 1
DOI: 10.1038/s41467-019-09989-y

The Cambridge Structural Database
journal, April 2016

Groom, Colin R.; Bruno, Ian J.; Lightfoot, Matthew P.
Acta Crystallographica Section B Structural Science, Crystal Engineering and Materials, Vol. 72, Issue 2, p. 171-179
DOI: 10.1107/S2052520616003954

Protein Structure and Function Prediction Using I‐TASSER
journal, December 2015

Yang, Jianyi; Zhang, Yang
Current Protocols in Bioinformatics, Vol. 52, Issue 1
DOI: 10.1002/0471250953.bi0508s52

The Scaffold Protein IscU Retains a Structured Conformation in the FeS Cluster Assembly Complex
journal, July 2014

Yan, Robert; Kelly, Geoff; Pastore, Annalisa
ChemBioChem, Vol. 15, Issue 11
DOI: 10.1002/cbic.201402211

The Human Iron–Sulfur Assembly Complex Catalyzes the Synthesis of [2Fe-2S] Clusters on ISCU2 That Can Be Transferred to Acceptor Molecules
journal, June 2015

Fox, Nicholas G.; Chakrabarti, Mrinmoy; McCormick, Sean P.
Biochemistry, Vol. 54, Issue 25
DOI: 10.1021/bi5014485

Yeast Frataxin Sequentially Chaperones and Stores Iron by Coupling Protein Assembly with Iron Oxidation
journal, May 2003

Park, Sungjo; Gakh, Oleksandr; O'Neill, Heather A.
Journal of Biological Chemistry, Vol. 278, Issue 33
DOI: 10.1074/jbc.M303158200

I-TASSER: a unified platform for automated protein structure and function prediction
journal, March 2010

Roy, Ambrish; Kucukural, Alper; Zhang, Yang
Nature Protocols, Vol. 5, Issue 4
DOI: 10.1038/nprot.2010.5

Zinc(II) binding on human wild-type ISCU and Met140 variants modulates NFS1 desulfurase activity
journal, September 2018

Fox, Nicholas G.; Martelli, Alain; Nabhan, Joseph F.
Biochimie, Vol. 152
DOI: 10.1016/j.biochi.2018.07.012

Physiologically relevant reconstitution of iron-sulfur cluster biosynthesis uncovers persulfide-processing functions of ferredoxin-2 and frataxin
journal, August 2019

Gervason, Sylvain; Larkem, Djabir; Mansour, Amir Ben
Nature Communications, Vol. 10, Issue 1
DOI: 10.1038/s41467-019-11470-9

Comparison of the Response of Bacterial IscU and SufU to Zn ²⁺ and Select Transition-Metal Ions
journal, December 2017

Roy, Poorna; Bauman, Mariia A.; Almutairi, Hayfa Habes
ACS Chemical Biology, Vol. 13, Issue 3
DOI: 10.1021/acschembio.7b00442

Iron–sulfur cluster biosynthesis and trafficking – impact on human disease conditions
journal, January 2018

Wachnowsky, C.; Fidai, I.; Cowan, J. A.
Metallomics, Vol. 10, Issue 1
DOI: 10.1039/C7MT00180K

Three-Dimensional Structure and Determinants of Stability of the Iron–Sulfur Cluster Scaffold Protein IscU from Escherichia coli
journal, July 2012

Kim, Jin Hae; Tonelli, Marco; Kim, Taewook
Biochemistry, Vol. 51, Issue 28
DOI: 10.1021/bi300579p

A Nonheme High-Spin Ferrous Pool in Mitochondria Isolated from Fermenting Saccharomyces cerevisiae
journal, May 2010

Holmes-Hampton, Gregory P.; Miao, Ren; Garber Morales, Jessica
Biochemistry, Vol. 49, Issue 19
DOI: 10.1021/bi1001823

A new metal binding domain involved in cadmium, cobalt and zinc transport
journal, July 2015

Smith, Aaron T.; Barupala, Dulmini; Stemmler, Timothy L.
Nature Chemical Biology, Vol. 11, Issue 9
DOI: 10.1038/nchembio.1863

The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis
journal, August 2016

Van Vranken, Jonathan G.; Jeong, Mi-Young; Wei, Peng
eLife, Vol. 5
DOI: 10.7554/eLife.17828

New Perspectives on Iron Uptake in Eukaryotes
journal, November 2018

Sherman, Harry G.; Jovanovic, Carolyn; Stolnik, Snow
Frontiers in Molecular Biosciences, Vol. 5
DOI: 10.3389/fmolb.2018.00097

Special issue on iron–sulfur proteins: Structure, function, biogenesis and diseases
journal, June 2015

Lill, Roland; Broderick, Joan B.; Dean, Dennis R.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1853, Issue 6
DOI: 10.1016/j.bbamcr.2015.03.001

Mitochondrial functional interactions between frataxin and Isu1p, the iron-sulfur cluster scaffold protein, in Saccharomyces cerevisiae
journal, December 2003

Ramazzotti, Anna; Vanmansart, Vincent; Foury, Françoise
FEBS Letters, Vol. 557, Issue 1-3
DOI: 10.1016/S0014-5793(03)01498-4

Structural characterization of an iron-sulfur cluster assembly protein IscU in a zinc-bound form
journal, April 2005

Liu, Jinyu; Oganesyan, Natalia; Shin, Dong-Hae
Proteins: Structure, Function, and Bioinformatics, Vol. 59, Issue 4
DOI: 10.1002/prot.20421

Solution NMR Structure of the Iron–Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active Site
journal, November 2004

Ramelot, Theresa A.; Cort, John R.; Goldsmith-Fischman, Sharon
Journal of Molecular Biology, Vol. 344, Issue 2
DOI: 10.1016/j.jmb.2004.08.038

Iron–sulfur cluster biosynthesis
journal, November 2008

Bandyopadhyay, Sibali; Chandramouli, Kala; Johnson, Michael K.
Biochemical Society Transactions, Vol. 36, Issue 6
DOI: 10.1042/BST0361112

Zinc Distribution and Expression Pattern of ZnT3 in Mouse Brain
journal, June 2007

Shen, Hui; Wang, Fudi; Zhang, Yuanxun
Biological Trace Element Research, Vol. 119, Issue 2
DOI: 10.1007/s12011-007-0056-2

Binding of Yeast Frataxin to the Scaffold for Fe-S Cluster Biogenesis, Isu
journal, March 2008

Wang, Tao; Craig, Elizabeth A.
Journal of Biological Chemistry, Vol. 283, Issue 18
DOI: 10.1074/jbc.M800399200

Order of Stability of Metal Complexes
journal, November 1948

Irving, H.; Williams, R. J. P.
Nature, Vol. 162, Issue 4123
DOI: 10.1038/162746a0

The Nfs1 interacting protein Isd11 has an essential role in Fe/S cluster biogenesis in mitochondria
journal, December 2005

Adam, Alexander C.; Bornhövd, Carsten; Prokisch, Holger
The EMBO Journal, Vol. 25, Issue 1
DOI: 10.1038/sj.emboj.7600905

Essential role of Isd11 in mitochondrial iron–sulfur cluster synthesis on Isu scaffold proteins
journal, December 2005

Wiedemann, Nils; Urzica, Eugen; Guiard, Bernard
The EMBO Journal, Vol. 25, Issue 1
DOI: 10.1038/sj.emboj.7600906

Metamorphic protein IscU alternates conformations in the course of its role as the scaffold protein for iron-sulfur cluster biosynthesis and delivery
journal, January 2013

Markley, John L.; Kim, Jin Hae; Dai, Ziqi
FEBS Letters, Vol. 587, Issue 8
DOI: 10.1016/j.febslet.2013.01.003

Cleavage and polyadenylation specificity factor 30: An RNA-binding zinc-finger protein with an unexpected 2Fe–2S cluster
journal, April 2016

Shimberg, Geoffrey D.; Michalek, Jamie L.; Oluyadi, Abdulafeez A.
Proceedings of the National Academy of Sciences, Vol. 113, Issue 17
DOI: 10.1073/pnas.1517620113

Iron loading site on the Fe–S cluster assembly scaffold protein is distinct from the active site
journal, March 2015

Rodrigues, Andria V.; Kandegedara, Ashoka; Rotondo, John A.
BioMetals, Vol. 28, Issue 3
DOI: 10.1007/s10534-015-9846-8

Recovery of mrs3Δmrs4Δ Saccharomyces cerevisiae Cells under Iron-Sufficient Conditions and the Role of Fe ₅₈₀
journal, January 2018

Moore, Michael J.; Wofford, Joshua D.; Dancis, Andrew
Biochemistry, Vol. 57, Issue 5
DOI: 10.1021/acs.biochem.7b01034

ISCU(M108I) and ISCU(D39V) Differ from Wild-Type ISCU in Their Failure To Form Cysteine Desulfurase Complexes Containing Both Frataxin and Ferredoxin
journal, February 2018

Cai, Kai; Frederick, Ronnie O.; Tonelli, Marco
Biochemistry, Vol. 57, Issue 9
DOI: 10.1021/acs.biochem.7b01234

Mammalian Frataxin: An Essential Function for Cellular Viability through an Interaction with a Preformed ISCU/NFS1/ISD11 Iron-Sulfur Assembly Complex
journal, January 2011

Schmucker, Stéphane; Martelli, Alain; Colin, Florent
PLoS ONE, Vol. 6, Issue 1
DOI: 10.1371/journal.pone.0016199

Analysis of Sulfur Biochemistry of Sulfur Bacteria Using X-ray Absorption Spectroscopy ^†
journal, July 2001

Pickering, Ingrid J.; George, Graham N.; Yu, Eileen Y.
Biochemistry, Vol. 40, Issue 27
DOI: 10.1021/bi0105532

Humans possess two mitochondrial ferredoxins, Fdx1 and Fdx2, with distinct roles in steroidogenesis, heme, and Fe/S cluster biosynthesis
journal, June 2010

Sheftel, A. D.; Stehling, O.; Pierik, A. J.
Proceedings of the National Academy of Sciences, Vol. 107, Issue 26
DOI: 10.1073/pnas.1004250107

Human Frataxin Activates Fe–S Cluster Biosynthesis by Facilitating Sulfur Transfer Chemistry
journal, July 2014

Bridwell-Rabb, Jennifer; Fox, Nicholas G.; Tsai, Chi-Lin
Biochemistry, Vol. 53, Issue 30
DOI: 10.1021/bi500532e

Frataxin Directly Stimulates Mitochondrial Cysteine Desulfurase by Exposing Substrate-binding Sites, and a Mutant Fe-S Cluster Scaffold Protein with Frataxin-bypassing Ability Acts Similarly
journal, November 2013

Pandey, Alok; Gordon, Donna M.; Pain, Jayashree
Journal of Biological Chemistry, Vol. 288, Issue 52
DOI: 10.1074/jbc.M113.525857

Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS
journal, March 2009

Adinolfi, Salvatore; Iannuzzi, Clara; Prischi, Filippo
Nature Structural & Molecular Biology, Vol. 16, Issue 4
DOI: 10.1038/nsmb.1579

Zinc Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli
journal, March 2019

Li, Jianghui; Ren, Xiaojun; Fan, Bingqian
Applied and Environmental Microbiology, Vol. 85, Issue 9
DOI: 10.1128/AEM.01967-18

Structure and Dynamics of the Iron−Sulfur Cluster Assembly Scaffold Protein IscU and Its Interaction with the Cochaperone HscB
journal, July 2009

Kim, Jin Hae; Füzéry, Anna K.; Tonelli, Marco
Biochemistry, Vol. 48, Issue 26
DOI: 10.1021/bi9002277

Structure of human Fe–S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP–ISD11 interactions
journal, June 2017

Cory, Seth A.; Van Vranken, Jonathan G.; Brignole, Edward J.
Proceedings of the National Academy of Sciences, Vol. 114, Issue 27
DOI: 10.1073/pnas.1702849114

Iron−Sulfur Cluster Biosynthesis: Characterization of Iron Nucleation Sites for Assembly of the [2Fe−2S] ²⁺ Cluster Core in IscU Proteins
journal, July 2002

Nuth, Manunya; Yoon, Taejin; Cowan, J. A.
Journal of the American Chemical Society, Vol. 124, Issue 30
DOI: 10.1021/ja0264596

Molecular Details of the Yeast Frataxin−Isu1 Interaction during Mitochondrial Fe−S Cluster Assembly
journal, October 2010

Cook, Jeremy D.; Kondapalli, Kalyan C.; Rawat, Swati
Biochemistry, Vol. 49, Issue 40
DOI: 10.1021/bi1008613

The role of zinc in the stability of the marginally stable IscU scaffold protein: The Stability Determinants of IscU
journal, July 2014

Iannuzzi, Clara; Adrover, Miquel; Puglisi, Rita
Protein Science, Vol. 23, Issue 9
DOI: 10.1002/pro.2501

X-ray Absorption Pre-Edge Studies of High-spin Iron(II) Complexes
journal, March 1995

Randall, Clayton R.; Shu, Lijin; Chiou, Yu-Min
Inorganic Chemistry, Vol. 34, Issue 5
DOI: 10.1021/ic00109a007

Turning Saccharomyces cerevisiae into a Frataxin-Independent Organism
journal, May 2015

Yoon, Heeyong; Knight, Simon A. B.; Pandey, Alok
PLOS Genetics, Vol. 11, Issue 5
DOI: 10.1371/journal.pgen.1005135

An interaction between frataxin and Isu1/Nfs1 that is crucial for Fe/S cluster synthesis on Isu1
journal, August 2003

Gerber, Jana; Mühlenhoff, Ulrich; Lill, Roland
EMBO reports, Vol. 4, Issue 9
DOI: 10.1038/sj.embor.embor918

Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex
journal, November 2017

Boniecki, Michal T.; Freibert, Sven A.; Mühlenhoff, Ulrich
Nature Communications, Vol. 8, Issue 1
DOI: 10.1038/s41467-017-01497-1

Monomeric Yeast Frataxin Is an Iron-Binding Protein ^†
journal, June 2006

Cook, Jeremy D.; Bencze, Krisztina Z.; Jankovic, Ana D.
Biochemistry, Vol. 45, Issue 25
DOI: 10.1021/bi060424r

Mapping iron binding sites on human frataxin: implications for cluster assembly on the ISU Fe–S cluster scaffold protein
journal, April 2008

Huang, Jia; Dizin, Eric; Cowan, J. A.
JBIC Journal of Biological Inorganic Chemistry, Vol. 13, Issue 5
DOI: 10.1007/s00775-008-0369-4

Iron−Sulfur Cluster Biosynthesis: Toward an Understanding of Cellular Machinery and Molecular Mechanism ^†
journal, September 2004

Mansy, Sheref S.; Cowan, J. A.
Accounts of Chemical Research, Vol. 37, Issue 9
DOI: 10.1021/ar0301781

Detection of Labile Low-Molecular-Mass Transition Metal Complexes in Mitochondria
journal, May 2015

McCormick, Sean P.; Moore, Michael J.; Lindahl, Paul A.
Biochemistry, Vol. 54, Issue 22
DOI: 10.1021/bi5015437

Iron Binding and Oxidation Kinetics in Frataxin CyaY of Escherichia coli
journal, August 2004

Bou-Abdallah, Fadi; Adinolfi, Salvatore; Pastore, Annalisa
Journal of Molecular Biology, Vol. 341, Issue 2
DOI: 10.1016/j.jmb.2004.05.072

Program DYNAFIT for the Analysis of Enzyme Kinetic Data: Application to HIV Proteinase
journal, June 1996

Kuzmič, Petr
Analytical Biochemistry, Vol. 237, Issue 2
DOI: 10.1006/abio.1996.0238

Iron–sulfur cluster biogenesis and trafficking in mitochondria
journal, June 2017

Braymer, Joseph J.; Lill, Roland
Journal of Biological Chemistry, Vol. 292, Issue 31
DOI: 10.1074/jbc.R117.787101

Yeast Frataxin Solution Structure, Iron Binding, and Ferrochelatase Interaction ^† ^, ^‡
journal, December 2004

He, Yanan; Alam, Steven L.; Proteasa, Simona V.
Biochemistry, Vol. 43, Issue 51
DOI: 10.1021/bi0488193

Characterization and Structure of a Zn ²⁺ and [2Fe-2S]-containing Copper Chaperone from Archaeoglobus fulgidus
journal, July 2007

Sazinsky, Matthew H.; LeMoine, Benjamin; Orofino, Maria
Journal of Biological Chemistry, Vol. 282, Issue 35
DOI: 10.1074/jbc.M703311200

The Asymmetric Trimeric Architecture of [2Fe–2S] IscU: Implications for Its Scaffolding during Iron–Sulfur Cluster Biosynthesis
journal, October 2008

Shimomura, Yoshimitsu; Wada, Kei; Fukuyama, Keiichi
Journal of Molecular Biology, Vol. 383, Issue 1
DOI: 10.1016/j.jmb.2008.08.015

The Yeast Scaffold Proteins Isu1p and Isu2p Are Required inside Mitochondria for Maturation of Cytosolic Fe/S Proteins
journal, May 2004

Gerber, J.; Neumann, K.; Prohl, C.
Molecular and Cellular Biology, Vol. 24, Issue 11
DOI: 10.1128/MCB.24.11.4848-4857.2004

Synthesis, delivery and regulation of eukaryotic heme and Fe–S cluster cofactors
journal, February 2016

Barupala, Dulmini P.; Dzul, Stephen P.; Riggs-Gelasco, Pamela Jo
Archives of Biochemistry and Biophysics, Vol. 592
DOI: 10.1016/j.abb.2016.01.010

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Title: Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”

Abstract

Citation Formats

Clustal Omega for making accurate alignments of many protein sequences: Clustal Omega for Many Protein Sequences journal, October 2017

Acidic residues of yeast frataxin have an essential role in Fe–S cluster assembly journal, December 2006

In vitro characterization of a novel Isu homologue from Drosophila melanogaster for de novo FeS-cluster formation journal, January 2017

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The Cambridge Structural Database journal, April 2016

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Zinc Distribution and Expression Pattern of ZnT3 in Mouse Brain journal, June 2007

Binding of Yeast Frataxin to the Scaffold for Fe-S Cluster Biogenesis, Isu journal, March 2008

Order of Stability of Metal Complexes journal, November 1948

The Nfs1 interacting protein Isd11 has an essential role in Fe/S cluster biogenesis in mitochondria journal, December 2005

Essential role of Isd11 in mitochondrial iron–sulfur cluster synthesis on Isu scaffold proteins journal, December 2005

Metamorphic protein IscU alternates conformations in the course of its role as the scaffold protein for iron-sulfur cluster biosynthesis and delivery journal, January 2013

Cleavage and polyadenylation specificity factor 30: An RNA-binding zinc-finger protein with an unexpected 2Fe–2S cluster journal, April 2016

Iron loading site on the Fe–S cluster assembly scaffold protein is distinct from the active site journal, March 2015

Recovery of mrs3Δmrs4Δ Saccharomyces cerevisiae Cells under Iron-Sufficient Conditions and the Role of Fe 580 journal, January 2018

ISCU(M108I) and ISCU(D39V) Differ from Wild-Type ISCU in Their Failure To Form Cysteine Desulfurase Complexes Containing Both Frataxin and Ferredoxin journal, February 2018

Mammalian Frataxin: An Essential Function for Cellular Viability through an Interaction with a Preformed ISCU/NFS1/ISD11 Iron-Sulfur Assembly Complex journal, January 2011

Analysis of Sulfur Biochemistry of Sulfur Bacteria Using X-ray Absorption Spectroscopy † journal, July 2001

Humans possess two mitochondrial ferredoxins, Fdx1 and Fdx2, with distinct roles in steroidogenesis, heme, and Fe/S cluster biosynthesis journal, June 2010

Human Frataxin Activates Fe–S Cluster Biosynthesis by Facilitating Sulfur Transfer Chemistry journal, July 2014

Frataxin Directly Stimulates Mitochondrial Cysteine Desulfurase by Exposing Substrate-binding Sites, and a Mutant Fe-S Cluster Scaffold Protein with Frataxin-bypassing Ability Acts Similarly journal, November 2013

Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS journal, March 2009

Zinc Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli journal, March 2019

Structure and Dynamics of the Iron−Sulfur Cluster Assembly Scaffold Protein IscU and Its Interaction with the Cochaperone HscB journal, July 2009

Structure of human Fe–S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP–ISD11 interactions journal, June 2017

Iron−Sulfur Cluster Biosynthesis: Characterization of Iron Nucleation Sites for Assembly of the [2Fe−2S] 2+ Cluster Core in IscU Proteins journal, July 2002

Molecular Details of the Yeast Frataxin−Isu1 Interaction during Mitochondrial Fe−S Cluster Assembly journal, October 2010

The role of zinc in the stability of the marginally stable IscU scaffold protein: The Stability Determinants of IscU journal, July 2014

X-ray Absorption Pre-Edge Studies of High-spin Iron(II) Complexes journal, March 1995

Turning Saccharomyces cerevisiae into a Frataxin-Independent Organism journal, May 2015

An interaction between frataxin and Isu1/Nfs1 that is crucial for Fe/S cluster synthesis on Isu1 journal, August 2003

Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex journal, November 2017

Monomeric Yeast Frataxin Is an Iron-Binding Protein † journal, June 2006

Mapping iron binding sites on human frataxin: implications for cluster assembly on the ISU Fe–S cluster scaffold protein journal, April 2008

Iron−Sulfur Cluster Biosynthesis: Toward an Understanding of Cellular Machinery and Molecular Mechanism † journal, September 2004

Detection of Labile Low-Molecular-Mass Transition Metal Complexes in Mitochondria journal, May 2015

Iron Binding and Oxidation Kinetics in Frataxin CyaY of Escherichia coli journal, August 2004

Program DYNAFIT for the Analysis of Enzyme Kinetic Data: Application to HIV Proteinase journal, June 1996

Iron–sulfur cluster biogenesis and trafficking in mitochondria journal, June 2017

Yeast Frataxin Solution Structure, Iron Binding, and Ferrochelatase Interaction † , ‡ journal, December 2004

Characterization and Structure of a Zn 2+ and [2Fe-2S]-containing Copper Chaperone from Archaeoglobus fulgidus journal, July 2007

The Asymmetric Trimeric Architecture of [2Fe–2S] IscU: Implications for Its Scaffolding during Iron–Sulfur Cluster Biosynthesis journal, October 2008

The Yeast Scaffold Proteins Isu1p and Isu2p Are Required inside Mitochondria for Maturation of Cytosolic Fe/S Proteins journal, May 2004

Synthesis, delivery and regulation of eukaryotic heme and Fe–S cluster cofactors journal, February 2016

Clustal Omega for making accurate alignments of many protein sequences: Clustal Omega for Many Protein Sequences
journal, October 2017

Acidic residues of yeast frataxin have an essential role in Fe–S cluster assembly
journal, December 2006

In vitro characterization of a novel Isu homologue from Drosophila melanogaster for de novo FeS-cluster formation
journal, January 2017

Structure and Ubiquitin Interactions of the Conserved Zinc Finger Domain of Npl4
journal, March 2003

Human Frataxin Is an Allosteric Switch That Activates the Fe−S Cluster Biosynthetic Complex
journal, November 2010

Regulation of Mitochondrial Iron Accumulation by Yfh1p, a Putative Homolog of Frataxin
journal, June 1997

Progress and challenges in the theory and interpretation of X-ray spectra
journal, March 2001

Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism
journal, May 2019

The Cambridge Structural Database
journal, April 2016

Protein Structure and Function Prediction Using I‐TASSER
journal, December 2015

The Scaffold Protein IscU Retains a Structured Conformation in the FeS Cluster Assembly Complex
journal, July 2014

The Human Iron–Sulfur Assembly Complex Catalyzes the Synthesis of [2Fe-2S] Clusters on ISCU2 That Can Be Transferred to Acceptor Molecules
journal, June 2015

Yeast Frataxin Sequentially Chaperones and Stores Iron by Coupling Protein Assembly with Iron Oxidation
journal, May 2003

I-TASSER: a unified platform for automated protein structure and function prediction
journal, March 2010

Zinc(II) binding on human wild-type ISCU and Met140 variants modulates NFS1 desulfurase activity
journal, September 2018

Physiologically relevant reconstitution of iron-sulfur cluster biosynthesis uncovers persulfide-processing functions of ferredoxin-2 and frataxin
journal, August 2019

Comparison of the Response of Bacterial IscU and SufU to Zn ²⁺ and Select Transition-Metal Ions
journal, December 2017

Iron–sulfur cluster biosynthesis and trafficking – impact on human disease conditions
journal, January 2018

Three-Dimensional Structure and Determinants of Stability of the Iron–Sulfur Cluster Scaffold Protein IscU from Escherichia coli
journal, July 2012

A Nonheme High-Spin Ferrous Pool in Mitochondria Isolated from Fermenting Saccharomyces cerevisiae
journal, May 2010

A new metal binding domain involved in cadmium, cobalt and zinc transport
journal, July 2015

The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis
journal, August 2016

New Perspectives on Iron Uptake in Eukaryotes
journal, November 2018

Special issue on iron–sulfur proteins: Structure, function, biogenesis and diseases
journal, June 2015

Mitochondrial functional interactions between frataxin and Isu1p, the iron-sulfur cluster scaffold protein, in Saccharomyces cerevisiae
journal, December 2003

Structural characterization of an iron-sulfur cluster assembly protein IscU in a zinc-bound form
journal, April 2005

Solution NMR Structure of the Iron–Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active Site
journal, November 2004

Iron–sulfur cluster biosynthesis
journal, November 2008

Zinc Distribution and Expression Pattern of ZnT3 in Mouse Brain
journal, June 2007

Binding of Yeast Frataxin to the Scaffold for Fe-S Cluster Biogenesis, Isu
journal, March 2008

Order of Stability of Metal Complexes
journal, November 1948

The Nfs1 interacting protein Isd11 has an essential role in Fe/S cluster biogenesis in mitochondria
journal, December 2005

Essential role of Isd11 in mitochondrial iron–sulfur cluster synthesis on Isu scaffold proteins
journal, December 2005

Metamorphic protein IscU alternates conformations in the course of its role as the scaffold protein for iron-sulfur cluster biosynthesis and delivery
journal, January 2013

Cleavage and polyadenylation specificity factor 30: An RNA-binding zinc-finger protein with an unexpected 2Fe–2S cluster
journal, April 2016

Iron loading site on the Fe–S cluster assembly scaffold protein is distinct from the active site
journal, March 2015

Recovery of mrs3Δmrs4Δ Saccharomyces cerevisiae Cells under Iron-Sufficient Conditions and the Role of Fe ₅₈₀
journal, January 2018

ISCU(M108I) and ISCU(D39V) Differ from Wild-Type ISCU in Their Failure To Form Cysteine Desulfurase Complexes Containing Both Frataxin and Ferredoxin
journal, February 2018

Mammalian Frataxin: An Essential Function for Cellular Viability through an Interaction with a Preformed ISCU/NFS1/ISD11 Iron-Sulfur Assembly Complex
journal, January 2011

Analysis of Sulfur Biochemistry of Sulfur Bacteria Using X-ray Absorption Spectroscopy ^†
journal, July 2001

Humans possess two mitochondrial ferredoxins, Fdx1 and Fdx2, with distinct roles in steroidogenesis, heme, and Fe/S cluster biosynthesis
journal, June 2010

Human Frataxin Activates Fe–S Cluster Biosynthesis by Facilitating Sulfur Transfer Chemistry
journal, July 2014

Frataxin Directly Stimulates Mitochondrial Cysteine Desulfurase by Exposing Substrate-binding Sites, and a Mutant Fe-S Cluster Scaffold Protein with Frataxin-bypassing Ability Acts Similarly
journal, November 2013

Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS
journal, March 2009

Zinc Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli
journal, March 2019

Structure and Dynamics of the Iron−Sulfur Cluster Assembly Scaffold Protein IscU and Its Interaction with the Cochaperone HscB
journal, July 2009

Structure of human Fe–S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP–ISD11 interactions
journal, June 2017

Iron−Sulfur Cluster Biosynthesis: Characterization of Iron Nucleation Sites for Assembly of the [2Fe−2S] ²⁺ Cluster Core in IscU Proteins
journal, July 2002

Molecular Details of the Yeast Frataxin−Isu1 Interaction during Mitochondrial Fe−S Cluster Assembly
journal, October 2010

The role of zinc in the stability of the marginally stable IscU scaffold protein: The Stability Determinants of IscU
journal, July 2014

X-ray Absorption Pre-Edge Studies of High-spin Iron(II) Complexes
journal, March 1995

Turning Saccharomyces cerevisiae into a Frataxin-Independent Organism
journal, May 2015

An interaction between frataxin and Isu1/Nfs1 that is crucial for Fe/S cluster synthesis on Isu1
journal, August 2003

Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex
journal, November 2017

Monomeric Yeast Frataxin Is an Iron-Binding Protein ^†
journal, June 2006

Mapping iron binding sites on human frataxin: implications for cluster assembly on the ISU Fe–S cluster scaffold protein
journal, April 2008

Iron−Sulfur Cluster Biosynthesis: Toward an Understanding of Cellular Machinery and Molecular Mechanism ^†
journal, September 2004

Detection of Labile Low-Molecular-Mass Transition Metal Complexes in Mitochondria
journal, May 2015

Iron Binding and Oxidation Kinetics in Frataxin CyaY of Escherichia coli
journal, August 2004

Program DYNAFIT for the Analysis of Enzyme Kinetic Data: Application to HIV Proteinase
journal, June 1996

Iron–sulfur cluster biogenesis and trafficking in mitochondria
journal, June 2017

Yeast Frataxin Solution Structure, Iron Binding, and Ferrochelatase Interaction ^† ^, ^‡
journal, December 2004

Characterization and Structure of a Zn ²⁺ and [2Fe-2S]-containing Copper Chaperone from Archaeoglobus fulgidus
journal, July 2007

The Asymmetric Trimeric Architecture of [2Fe–2S] IscU: Implications for Its Scaffolding during Iron–Sulfur Cluster Biosynthesis
journal, October 2008

The Yeast Scaffold Proteins Isu1p and Isu2p Are Required inside Mitochondria for Maturation of Cytosolic Fe/S Proteins
journal, May 2004

Synthesis, delivery and regulation of eukaryotic heme and Fe–S cluster cofactors
journal, February 2016