skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”

Abstract

Model for Zn association to the active site of yeast Fe–S cluster assembly scaffold protein Isu1.

Authors:
 [1];  [1];  [1];  [2];  [2];  [2]; ORCiD logo [1]
+ Show Author Affiliations
  1. Department of Pharmaceutical Science, Wayne State University, Detroit, USA
  2. Department of Chemistry, The Ohio State University, Columbus, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1562561
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Metallomics
Additional Journal Information:
Journal Name: Metallomics Journal Volume: 11 Journal Issue: 11; Journal ID: ISSN 1756-5901
Publisher:
Royal Society of Chemistry (RSC)
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Lewis, Brianne E., Mason, Zachary, Rodrigues, Andria V., Nuth, Manunya, Dizin, Eric, Cowan, J. A., and Stemmler, Timothy L. Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”. United Kingdom: N. p., 2019. Web. doi:10.1039/C9MT00172G.
Copy to clipboard
Lewis, Brianne E., Mason, Zachary, Rodrigues, Andria V., Nuth, Manunya, Dizin, Eric, Cowan, J. A., & Stemmler, Timothy L. Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”. United Kingdom. doi:10.1039/C9MT00172G.
Copy to clipboard
Lewis, Brianne E., Mason, Zachary, Rodrigues, Andria V., Nuth, Manunya, Dizin, Eric, Cowan, J. A., and Stemmler, Timothy L. Wed . "Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”". United Kingdom. doi:10.1039/C9MT00172G.
Copy to clipboard
@article{osti_1562561,
title = {Unique roles of iron and zinc binding to the yeast Fe–S cluster scaffold assembly protein “Isu1”},
author = {Lewis, Brianne E. and Mason, Zachary and Rodrigues, Andria V. and Nuth, Manunya and Dizin, Eric and Cowan, J. A. and Stemmler, Timothy L.},
abstractNote = {Model for Zn association to the active site of yeast Fe–S cluster assembly scaffold protein Isu1.},
doi = {10.1039/C9MT00172G},
journal = {Metallomics},
number = 11,
volume = 11,
place = {United Kingdom},
year = {2019},
month = {11}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Accepted Manuscript (Publisher)
Publisher's Version of Record
DOI: 10.1039/C9MT00172G
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Clustal Omega for making accurate alignments of many protein sequences: Clustal Omega for Many Protein Sequences
journal, October 2017

  • Sievers, Fabian; Higgins, Desmond G.
  • Protein Science, Vol. 27, Issue 1
  • DOI: 10.1002/pro.3290

Acidic residues of yeast frataxin have an essential role in Fe–S cluster assembly
journal, December 2006

In vitro characterization of a novel Isu homologue from Drosophila melanogaster for de novo FeS-cluster formation
journal, January 2017

  • Dzul, Stephen P.; Rocha, Agostinho G.; Rawat, Swati
  • Metallomics, Vol. 9, Issue 1
  • DOI: 10.1039/C6MT00163G

Structure and Ubiquitin Interactions of the Conserved Zinc Finger Domain of Npl4
journal, March 2003

  • Wang, Bin; Alam, Steven L.; Meyer, Hemmo H.
  • Journal of Biological Chemistry, Vol. 278, Issue 22
  • DOI: 10.1074/jbc.M300459200

Human Frataxin Is an Allosteric Switch That Activates the Fe−S Cluster Biosynthetic Complex
journal, November 2010

  • Tsai, Chi-Lin; Barondeau, David P.
  • Biochemistry, Vol. 49, Issue 43
  • DOI: 10.1021/bi1013062

Progress and challenges in the theory and interpretation of X-ray spectra
journal, March 2001

Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism
journal, May 2019

The Cambridge Structural Database
journal, April 2016

  • Groom, Colin R.; Bruno, Ian J.; Lightfoot, Matthew P.
  • Acta Crystallographica Section B Structural Science, Crystal Engineering and Materials, Vol. 72, Issue 2, p. 171-179
  • DOI: 10.1107/S2052520616003954

The Scaffold Protein IscU Retains a Structured Conformation in the FeS Cluster Assembly Complex
journal, July 2014

The Human Iron–Sulfur Assembly Complex Catalyzes the Synthesis of [2Fe-2S] Clusters on ISCU2 That Can Be Transferred to Acceptor Molecules
journal, June 2015

  • Fox, Nicholas G.; Chakrabarti, Mrinmoy; McCormick, Sean P.
  • Biochemistry, Vol. 54, Issue 25
  • DOI: 10.1021/bi5014485

Yeast Frataxin Sequentially Chaperones and Stores Iron by Coupling Protein Assembly with Iron Oxidation
journal, May 2003

  • Park, Sungjo; Gakh, Oleksandr; O'Neill, Heather A.
  • Journal of Biological Chemistry, Vol. 278, Issue 33
  • DOI: 10.1074/jbc.M303158200

I-TASSER: a unified platform for automated protein structure and function prediction
journal, March 2010

  • Roy, Ambrish; Kucukural, Alper; Zhang, Yang
  • Nature Protocols, Vol. 5, Issue 4
  • DOI: 10.1038/nprot.2010.5

Zinc(II) binding on human wild-type ISCU and Met140 variants modulates NFS1 desulfurase activity
journal, September 2018

Physiologically relevant reconstitution of iron-sulfur cluster biosynthesis uncovers persulfide-processing functions of ferredoxin-2 and frataxin
journal, August 2019

Comparison of the Response of Bacterial IscU and SufU to Zn 2+ and Select Transition-Metal Ions
journal, December 2017

Iron–sulfur cluster biosynthesis and trafficking – impact on human disease conditions
journal, January 2018

  • Wachnowsky, C.; Fidai, I.; Cowan, J. A.
  • Metallomics, Vol. 10, Issue 1
  • DOI: 10.1039/C7MT00180K

Three-Dimensional Structure and Determinants of Stability of the Iron–Sulfur Cluster Scaffold Protein IscU from Escherichia coli
journal, July 2012

  • Kim, Jin Hae; Tonelli, Marco; Kim, Taewook
  • Biochemistry, Vol. 51, Issue 28
  • DOI: 10.1021/bi300579p

A Nonheme High-Spin Ferrous Pool in Mitochondria Isolated from Fermenting Saccharomyces cerevisiae
journal, May 2010

  • Holmes-Hampton, Gregory P.; Miao, Ren; Garber Morales, Jessica
  • Biochemistry, Vol. 49, Issue 19
  • DOI: 10.1021/bi1001823

A new metal binding domain involved in cadmium, cobalt and zinc transport
journal, July 2015

  • Smith, Aaron T.; Barupala, Dulmini; Stemmler, Timothy L.
  • Nature Chemical Biology, Vol. 11, Issue 9
  • DOI: 10.1038/nchembio.1863

New Perspectives on Iron Uptake in Eukaryotes
journal, November 2018

  • Sherman, Harry G.; Jovanovic, Carolyn; Stolnik, Snow
  • Frontiers in Molecular Biosciences, Vol. 5
  • DOI: 10.3389/fmolb.2018.00097

Special issue on iron–sulfur proteins: Structure, function, biogenesis and diseases
journal, June 2015

  • Lill, Roland; Broderick, Joan B.; Dean, Dennis R.
  • Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1853, Issue 6
  • DOI: 10.1016/j.bbamcr.2015.03.001

Mitochondrial functional interactions between frataxin and Isu1p, the iron-sulfur cluster scaffold protein, in Saccharomyces cerevisiae
journal, December 2003

Structural characterization of an iron-sulfur cluster assembly protein IscU in a zinc-bound form
journal, April 2005

  • Liu, Jinyu; Oganesyan, Natalia; Shin, Dong-Hae
  • Proteins: Structure, Function, and Bioinformatics, Vol. 59, Issue 4
  • DOI: 10.1002/prot.20421

Solution NMR Structure of the Iron–Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active Site
journal, November 2004

  • Ramelot, Theresa A.; Cort, John R.; Goldsmith-Fischman, Sharon
  • Journal of Molecular Biology, Vol. 344, Issue 2
  • DOI: 10.1016/j.jmb.2004.08.038

Iron–sulfur cluster biosynthesis
journal, November 2008

  • Bandyopadhyay, Sibali; Chandramouli, Kala; Johnson, Michael K.
  • Biochemical Society Transactions, Vol. 36, Issue 6
  • DOI: 10.1042/BST0361112

Zinc Distribution and Expression Pattern of ZnT3 in Mouse Brain
journal, June 2007

Binding of Yeast Frataxin to the Scaffold for Fe-S Cluster Biogenesis, Isu
journal, March 2008

  • Wang, Tao; Craig, Elizabeth A.
  • Journal of Biological Chemistry, Vol. 283, Issue 18
  • DOI: 10.1074/jbc.M800399200

Order of Stability of Metal Complexes
journal, November 1948

  • Irving, H.; Williams, R. J. P.
  • Nature, Vol. 162, Issue 4123
  • DOI: 10.1038/162746a0

The Nfs1 interacting protein Isd11 has an essential role in Fe/S cluster biogenesis in mitochondria
journal, December 2005

  • Adam, Alexander C.; Bornhövd, Carsten; Prokisch, Holger
  • The EMBO Journal, Vol. 25, Issue 1
  • DOI: 10.1038/sj.emboj.7600905

Essential role of Isd11 in mitochondrial iron–sulfur cluster synthesis on Isu scaffold proteins
journal, December 2005

Cleavage and polyadenylation specificity factor 30: An RNA-binding zinc-finger protein with an unexpected 2Fe–2S cluster
journal, April 2016

  • Shimberg, Geoffrey D.; Michalek, Jamie L.; Oluyadi, Abdulafeez A.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 17
  • DOI: 10.1073/pnas.1517620113

Iron loading site on the Fe–S cluster assembly scaffold protein is distinct from the active site
journal, March 2015

Recovery of mrs3Δmrs4Δ Saccharomyces cerevisiae Cells under Iron-Sufficient Conditions and the Role of Fe 580
journal, January 2018

Analysis of Sulfur Biochemistry of Sulfur Bacteria Using X-ray Absorption Spectroscopy †
journal, July 2001

  • Pickering, Ingrid J.; George, Graham N.; Yu, Eileen Y.
  • Biochemistry, Vol. 40, Issue 27
  • DOI: 10.1021/bi0105532

Humans possess two mitochondrial ferredoxins, Fdx1 and Fdx2, with distinct roles in steroidogenesis, heme, and Fe/S cluster biosynthesis
journal, June 2010

  • Sheftel, A. D.; Stehling, O.; Pierik, A. J.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 26
  • DOI: 10.1073/pnas.1004250107

Human Frataxin Activates Fe–S Cluster Biosynthesis by Facilitating Sulfur Transfer Chemistry
journal, July 2014

  • Bridwell-Rabb, Jennifer; Fox, Nicholas G.; Tsai, Chi-Lin
  • Biochemistry, Vol. 53, Issue 30
  • DOI: 10.1021/bi500532e

Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS
journal, March 2009

  • Adinolfi, Salvatore; Iannuzzi, Clara; Prischi, Filippo
  • Nature Structural & Molecular Biology, Vol. 16, Issue 4
  • DOI: 10.1038/nsmb.1579

Zinc Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli
journal, March 2019

  • Li, Jianghui; Ren, Xiaojun; Fan, Bingqian
  • Applied and Environmental Microbiology, Vol. 85, Issue 9
  • DOI: 10.1128/AEM.01967-18

Structure and Dynamics of the Iron−Sulfur Cluster Assembly Scaffold Protein IscU and Its Interaction with the Cochaperone HscB
journal, July 2009

  • Kim, Jin Hae; Füzéry, Anna K.; Tonelli, Marco
  • Biochemistry, Vol. 48, Issue 26
  • DOI: 10.1021/bi9002277

Structure of human Fe–S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP–ISD11 interactions
journal, June 2017

  • Cory, Seth A.; Van Vranken, Jonathan G.; Brignole, Edward J.
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 27
  • DOI: 10.1073/pnas.1702849114

Iron−Sulfur Cluster Biosynthesis:  Characterization of Iron Nucleation Sites for Assembly of the [2Fe−2S] 2+ Cluster Core in IscU Proteins
journal, July 2002

  • Nuth, Manunya; Yoon, Taejin; Cowan, J. A.
  • Journal of the American Chemical Society, Vol. 124, Issue 30
  • DOI: 10.1021/ja0264596

Molecular Details of the Yeast Frataxin−Isu1 Interaction during Mitochondrial Fe−S Cluster Assembly
journal, October 2010

  • Cook, Jeremy D.; Kondapalli, Kalyan C.; Rawat, Swati
  • Biochemistry, Vol. 49, Issue 40
  • DOI: 10.1021/bi1008613

The role of zinc in the stability of the marginally stable IscU scaffold protein: The Stability Determinants of IscU
journal, July 2014

  • Iannuzzi, Clara; Adrover, Miquel; Puglisi, Rita
  • Protein Science, Vol. 23, Issue 9
  • DOI: 10.1002/pro.2501

X-ray Absorption Pre-Edge Studies of High-spin Iron(II) Complexes
journal, March 1995

  • Randall, Clayton R.; Shu, Lijin; Chiou, Yu-Min
  • Inorganic Chemistry, Vol. 34, Issue 5
  • DOI: 10.1021/ic00109a007

Turning Saccharomyces cerevisiae into a Frataxin-Independent Organism
journal, May 2015

An interaction between frataxin and Isu1/Nfs1 that is crucial for Fe/S cluster synthesis on Isu1
journal, August 2003

Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex
journal, November 2017

  • Boniecki, Michal T.; Freibert, Sven A.; Mühlenhoff, Ulrich
  • Nature Communications, Vol. 8, Issue 1
  • DOI: 10.1038/s41467-017-01497-1

Monomeric Yeast Frataxin Is an Iron-Binding Protein †
journal, June 2006

  • Cook, Jeremy D.; Bencze, Krisztina Z.; Jankovic, Ana D.
  • Biochemistry, Vol. 45, Issue 25
  • DOI: 10.1021/bi060424r

Mapping iron binding sites on human frataxin: implications for cluster assembly on the ISU Fe–S cluster scaffold protein
journal, April 2008

  • Huang, Jia; Dizin, Eric; Cowan, J. A.
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 13, Issue 5
  • DOI: 10.1007/s00775-008-0369-4

Iron−Sulfur Cluster Biosynthesis:  Toward an Understanding of Cellular Machinery and Molecular Mechanism †
journal, September 2004

  • Mansy, Sheref S.; Cowan, J. A.
  • Accounts of Chemical Research, Vol. 37, Issue 9
  • DOI: 10.1021/ar0301781

Detection of Labile Low-Molecular-Mass Transition Metal Complexes in Mitochondria
journal, May 2015

  • McCormick, Sean P.; Moore, Michael J.; Lindahl, Paul A.
  • Biochemistry, Vol. 54, Issue 22
  • DOI: 10.1021/bi5015437

Iron Binding and Oxidation Kinetics in Frataxin CyaY of Escherichia coli
journal, August 2004

  • Bou-Abdallah, Fadi; Adinolfi, Salvatore; Pastore, Annalisa
  • Journal of Molecular Biology, Vol. 341, Issue 2
  • DOI: 10.1016/j.jmb.2004.05.072

Program DYNAFIT for the Analysis of Enzyme Kinetic Data: Application to HIV Proteinase
journal, June 1996

Iron–sulfur cluster biogenesis and trafficking in mitochondria
journal, June 2017

  • Braymer, Joseph J.; Lill, Roland
  • Journal of Biological Chemistry, Vol. 292, Issue 31
  • DOI: 10.1074/jbc.R117.787101

Yeast Frataxin Solution Structure, Iron Binding, and Ferrochelatase Interaction † , ‡
journal, December 2004

  • He, Yanan; Alam, Steven L.; Proteasa, Simona V.
  • Biochemistry, Vol. 43, Issue 51
  • DOI: 10.1021/bi0488193

Characterization and Structure of a Zn 2+ and [2Fe-2S]-containing Copper Chaperone from Archaeoglobus fulgidus
journal, July 2007

  • Sazinsky, Matthew H.; LeMoine, Benjamin; Orofino, Maria
  • Journal of Biological Chemistry, Vol. 282, Issue 35
  • DOI: 10.1074/jbc.M703311200

The Asymmetric Trimeric Architecture of [2Fe–2S] IscU: Implications for Its Scaffolding during Iron–Sulfur Cluster Biosynthesis
journal, October 2008

  • Shimomura, Yoshimitsu; Wada, Kei; Fukuyama, Keiichi
  • Journal of Molecular Biology, Vol. 383, Issue 1
  • DOI: 10.1016/j.jmb.2008.08.015

The Yeast Scaffold Proteins Isu1p and Isu2p Are Required inside Mitochondria for Maturation of Cytosolic Fe/S Proteins
journal, May 2004

Synthesis, delivery and regulation of eukaryotic heme and Fe–S cluster cofactors
journal, February 2016

  • Barupala, Dulmini P.; Dzul, Stephen P.; Riggs-Gelasco, Pamela Jo
  • Archives of Biochemistry and Biophysics, Vol. 592
  • DOI: 10.1016/j.abb.2016.01.010

Protein Structure and Function Prediction Using I‐TASSER
journal, December 2015

    [ × clear filter / sort ]

    Similar Records in DOE PAGES and OSTI.GOV collections: