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Title: A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos

Abstract

In view of our rapidly growing demands on vegetable oil, enhancing fatty acid synthesis in maize (Zea mays) represents tremendous nutritional and economic benefits. In corn kernels, the endosperm and the embryo are the main site for synthesis and accumulation of starch and oil, respectively. So far, breeding efforts to achieve elevated oil content in maize resulted in smaller endosperm and therefore lower yield. It is hypothesized that the key to increasing oil content in maize kernels without affecting the yield lies on directly changing their carbon metabolism. To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48 and 34% of oil, respectively. Comparative metabolomics highlighted the metabolites and pathways that were active in maize embryos and important for oil production. The contribution of each pathway to fatty acid synthesis in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-Metabolic Flux Analysis) in developing Alex embryos to build a map of carbon flow through central metabolism. This approach combined mathematical modeling with biochemical quantification to identify metabolic bottlenecks in fatty acid synthesismore » in maize embryos. This study hence describes the combination of innovative tools that will pave the way for controlling seed composition in important food crops.« less

Authors:
ORCiD logo [1]; ORCiD logo [2];  [3];  [4]; ORCiD logo [1]
  1. BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, Texas 76203
  2. Laboratoire Transformations Intégrées de la Matière Renouvelable (Université de Technologie de Compiègne/École Supérieure de Chimie Organique et Minérale, Équipe d'Accueil 4297 Transformations Integrées de la Matière Renouvelable), Centre de Recherche de Royallieu, Université de Technologie de Compiègne, course spéciale 60319, F-60203 Compiègne cedex, France
  3. Center for Applied Plant Sciences, The Ohio State University, Columbus, Ohio 43210
  4. Ohio University Heritage College of Osteopathic Medicine, Dublin, Ohio 43016
Publication Date:
Research Org.:
Univ. of North Texas, Denton, TX (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1562539
Alternate Identifier(s):
OSTI ID: 1658577
Grant/Contract Number:  
SC0019233
Resource Type:
Published Article
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Name: Plant Physiology (Bethesda) Journal Volume: 181 Journal Issue: 3; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Fatty acid synthesis; Isotopic labeling; Metabolic Flux Analysis; Plastidic malic enzyme; Zea mays.

Citation Formats

Cocuron, Jean-Christophe, Koubaa, Mohamed, Kimmelfield, Rebecca, Ross, Zacchary, and Alonso, Ana Paula. A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos. United States: N. p., 2019. Web. doi:10.1104/pp.19.00920.
Cocuron, Jean-Christophe, Koubaa, Mohamed, Kimmelfield, Rebecca, Ross, Zacchary, & Alonso, Ana Paula. A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos. United States. doi:https://doi.org/10.1104/pp.19.00920
Cocuron, Jean-Christophe, Koubaa, Mohamed, Kimmelfield, Rebecca, Ross, Zacchary, and Alonso, Ana Paula. Tue . "A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos". United States. doi:https://doi.org/10.1104/pp.19.00920.
@article{osti_1562539,
title = {A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos},
author = {Cocuron, Jean-Christophe and Koubaa, Mohamed and Kimmelfield, Rebecca and Ross, Zacchary and Alonso, Ana Paula},
abstractNote = {In view of our rapidly growing demands on vegetable oil, enhancing fatty acid synthesis in maize (Zea mays) represents tremendous nutritional and economic benefits. In corn kernels, the endosperm and the embryo are the main site for synthesis and accumulation of starch and oil, respectively. So far, breeding efforts to achieve elevated oil content in maize resulted in smaller endosperm and therefore lower yield. It is hypothesized that the key to increasing oil content in maize kernels without affecting the yield lies on directly changing their carbon metabolism. To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48 and 34% of oil, respectively. Comparative metabolomics highlighted the metabolites and pathways that were active in maize embryos and important for oil production. The contribution of each pathway to fatty acid synthesis in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-Metabolic Flux Analysis) in developing Alex embryos to build a map of carbon flow through central metabolism. This approach combined mathematical modeling with biochemical quantification to identify metabolic bottlenecks in fatty acid synthesis in maize embryos. This study hence describes the combination of innovative tools that will pave the way for controlling seed composition in important food crops.},
doi = {10.1104/pp.19.00920},
journal = {Plant Physiology (Bethesda)},
number = 3,
volume = 181,
place = {United States},
year = {2019},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: https://doi.org/10.1104/pp.19.00920

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Cited by: 5 works
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Works referencing / citing this record:

13C-Metabolic Flux Analysis in Developing Flax (Linum usitatissinum L.) Embryos to Understand Storage Lipid Biosynthesis
journal, December 2019

  • Acket, Sébastien; Degournay, Anthony; Rossez, Yannick
  • Metabolites, Vol. 10, Issue 1
  • DOI: 10.3390/metabo10010014

Liquid Chromatography Tandem Mass Spectrometry Quantification of 13C-Labeling in Sugars
journal, January 2020

  • Cocuron, Jean-Christophe; Ross, Zacchary; Alonso, Ana P.
  • Metabolites, Vol. 10, Issue 1
  • DOI: 10.3390/metabo10010030