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Title: Dynamic super-resolution structured illumination imaging in the living brain

Abstract

Cells in the brain act as components of extended networks. Therefore, to understand neurobiological processes in a physiological context, it is essential to study them in vivo. Super-resolution microscopy has spatial resolution beyond the diffraction limit, thus promising to provide structural and functional insights that are not accessible with conventional microscopy. However, to apply it to in vivo brain imaging, we must address the challenges of 3D imaging in an optically heterogeneous tissue that is constantly in motion. We optimized image acquisition and reconstruction to combat sample motion and applied adaptive optics to correcting sample-induced optical aberrations in super-resolution structured illumination microscopy (SIM) in vivo. Lastly, we imaged the brains of live zebrafish larvae and mice and observed the dynamics of dendrites and dendritic spines at nanoscale resolution.

Authors:
ORCiD logo [1];  [2];  [2];  [3];  [3]; ORCiD logo [2]; ORCiD logo [4];  [4]
  1. Howard Hughes Medical Inst., Ashburn, VA (United States). Janelia Research Campus; Univ. of California, Berkeley, CA (United States). Dept. of Physics
  2. Howard Hughes Medical Inst., Ashburn, VA (United States). Janelia Research Campus
  3. Univ. of California, Berkeley, CA (United States). Dept. of Physics
  4. Howard Hughes Medical Inst., Ashburn, VA (United States). Janelia Research Campus; Univ. of California, Berkeley, CA (United States). Dept. of Physics, Dept. of Molecular & Cell Biology, Helen Wills Neuroscience Inst.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1561913
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 116; Journal Issue: 19; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; super-resolution; adaptive optics; brain imaging; in vivo; synapses

Citation Formats

Turcotte, Raphaël, Liang, Yajie, Tanimoto, Masashi, Zhang, Qinrong, Li, Ziwei, Koyama, Minoru, Betzig, Eric, and Ji, Na. Dynamic super-resolution structured illumination imaging in the living brain. United States: N. p., 2019. Web. doi:10.1073/pnas.1819965116.
Turcotte, Raphaël, Liang, Yajie, Tanimoto, Masashi, Zhang, Qinrong, Li, Ziwei, Koyama, Minoru, Betzig, Eric, & Ji, Na. Dynamic super-resolution structured illumination imaging in the living brain. United States. doi:10.1073/pnas.1819965116.
Turcotte, Raphaël, Liang, Yajie, Tanimoto, Masashi, Zhang, Qinrong, Li, Ziwei, Koyama, Minoru, Betzig, Eric, and Ji, Na. Fri . "Dynamic super-resolution structured illumination imaging in the living brain". United States. doi:10.1073/pnas.1819965116. https://www.osti.gov/servlets/purl/1561913.
@article{osti_1561913,
title = {Dynamic super-resolution structured illumination imaging in the living brain},
author = {Turcotte, Raphaël and Liang, Yajie and Tanimoto, Masashi and Zhang, Qinrong and Li, Ziwei and Koyama, Minoru and Betzig, Eric and Ji, Na},
abstractNote = {Cells in the brain act as components of extended networks. Therefore, to understand neurobiological processes in a physiological context, it is essential to study them in vivo. Super-resolution microscopy has spatial resolution beyond the diffraction limit, thus promising to provide structural and functional insights that are not accessible with conventional microscopy. However, to apply it to in vivo brain imaging, we must address the challenges of 3D imaging in an optically heterogeneous tissue that is constantly in motion. We optimized image acquisition and reconstruction to combat sample motion and applied adaptive optics to correcting sample-induced optical aberrations in super-resolution structured illumination microscopy (SIM) in vivo. Lastly, we imaged the brains of live zebrafish larvae and mice and observed the dynamics of dendrites and dendritic spines at nanoscale resolution.},
doi = {10.1073/pnas.1819965116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 19,
volume = 116,
place = {United States},
year = {2019},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 1 work
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Figures / Tables:

Fig. 1 Fig. 1: AO is essential for SIM imaging in brain tissue. (A–D) Images of dendrites at a depth of 25 μm in a cortical slice of a Thy1-GFP line M mouse (A and B) without and (C and D) with AO. (Scale bars: 5 μm; Inset widths: A and C,more » 3 μm; B and D, 2 μm.) (E and F) Line profiles of (E) a spine head and (F) a spine neck with and without AO as identified by the lines in B and D. Images were normalized to the AO condition.« less

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