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Title: Demonstration of the potential of Picochlorum soloecismus as a microalgal platform for the production of renewable fuels

Abstract

Photosynthetic microbes are considered promising biofactories for transforming inorganic carbon from the atmosphere into a renewable source of chemicals and precursors of industrial interest; however, there continues to be a need for strains that demonstrate high productivity, environmental robustness, and the potential to be genetically manipulated. Genome sequencing and biochemical characterization of promising culture collection microalgae strains, as well as the isolation of previously unidentified strains from the environment or mixed cultures, bring us closer to the goal of decreasing the cost-per-gallon of algal biofuels by identifying new and promising potential production strains. The halotolerant alga Picochlorum soloecismus was isolated from the culture collection strain, Nannochloropsis salina CCMP 1776. Here, we show that P. soloecismus accumulates moderate levels of fatty acids and high levels of total carbohydrates and that it can effectively grow in a range of salinities. In addition, we make use of its sequenced genome to compare it to other biofuel production platforms and to validate the capacity for engineering this strain's genome. Furthermore, our work shows that Picochlorum soloecismus is a candidate production strain for the generation of renewable bioproducts.

Authors:
ORCiD logo [1];  [1]; ORCiD logo [1];  [1]; ORCiD logo [1];  [2];  [3]; ORCiD logo [1]; ORCiD logo [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. Michigan State Univ., East Lansing, MI (United States)
  3. Michigan State Univ., East Lansing, MI (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Berkeley Synthetic Biology Institute, Berkeley, CA (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1561324
Alternate Identifier(s):
OSTI ID: 1569615
Report Number(s):
LA-UR-18-31775
Journal ID: ISSN 2211-9264
Grant/Contract Number:  
89233218CNA000001; NL0025841; EE0003046; FG02-91ER20021
Resource Type:
Published Article
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 43; Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Biological Science; Picochlorum; Biofuel feedstock; Halotolerant; Genetic engineering; Genome analysis

Citation Formats

Gonzalez-Esquer, Cesar Raul, Wright, Kimberly Theresa, Sudasinghe, Nilusha, Carr, Carol Kay, Sanders, Claire Kathleen, Turmo, Aiko, Kerfeld, Cheryl A., Twary, Scott Nicholas, and Dale, Taraka T. Demonstration of the potential of Picochlorum soloecismus as a microalgal platform for the production of renewable fuels. United States: N. p., 2019. Web. doi:10.1016/j.algal.2019.101658.
Gonzalez-Esquer, Cesar Raul, Wright, Kimberly Theresa, Sudasinghe, Nilusha, Carr, Carol Kay, Sanders, Claire Kathleen, Turmo, Aiko, Kerfeld, Cheryl A., Twary, Scott Nicholas, & Dale, Taraka T. Demonstration of the potential of Picochlorum soloecismus as a microalgal platform for the production of renewable fuels. United States. doi:10.1016/j.algal.2019.101658.
Gonzalez-Esquer, Cesar Raul, Wright, Kimberly Theresa, Sudasinghe, Nilusha, Carr, Carol Kay, Sanders, Claire Kathleen, Turmo, Aiko, Kerfeld, Cheryl A., Twary, Scott Nicholas, and Dale, Taraka T. Wed . "Demonstration of the potential of Picochlorum soloecismus as a microalgal platform for the production of renewable fuels". United States. doi:10.1016/j.algal.2019.101658.
@article{osti_1561324,
title = {Demonstration of the potential of Picochlorum soloecismus as a microalgal platform for the production of renewable fuels},
author = {Gonzalez-Esquer, Cesar Raul and Wright, Kimberly Theresa and Sudasinghe, Nilusha and Carr, Carol Kay and Sanders, Claire Kathleen and Turmo, Aiko and Kerfeld, Cheryl A. and Twary, Scott Nicholas and Dale, Taraka T.},
abstractNote = {Photosynthetic microbes are considered promising biofactories for transforming inorganic carbon from the atmosphere into a renewable source of chemicals and precursors of industrial interest; however, there continues to be a need for strains that demonstrate high productivity, environmental robustness, and the potential to be genetically manipulated. Genome sequencing and biochemical characterization of promising culture collection microalgae strains, as well as the isolation of previously unidentified strains from the environment or mixed cultures, bring us closer to the goal of decreasing the cost-per-gallon of algal biofuels by identifying new and promising potential production strains. The halotolerant alga Picochlorum soloecismus was isolated from the culture collection strain, Nannochloropsis salina CCMP 1776. Here, we show that P. soloecismus accumulates moderate levels of fatty acids and high levels of total carbohydrates and that it can effectively grow in a range of salinities. In addition, we make use of its sequenced genome to compare it to other biofuel production platforms and to validate the capacity for engineering this strain's genome. Furthermore, our work shows that Picochlorum soloecismus is a candidate production strain for the generation of renewable bioproducts.},
doi = {10.1016/j.algal.2019.101658},
journal = {Algal Research},
number = C,
volume = 43,
place = {United States},
year = {2019},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1016/j.algal.2019.101658

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