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Title: Soil bacterial and fungal response to wildfires in the Canadian boreal forest across a burn severity gradient

Abstract

Global fire regimes are changing, with increases in wildfire frequency and severity expected for many North American forests over the next 100 years. Fires can result in dramatic changes to carbon (C) stocks and can restructure plant and microbial communities, with long-lasting effects on ecosystem functions. We investigated wildfire effects on soil microbial communities (bacteria and fungi) in an extreme fire season in the northwestern Canadian boreal forest, using field surveys, remote sensing, and high-throughput amplicon sequencing in upland and wetland sites. We hypothesized that vegetation community and soil pH would be the most important determinants of microbial community composition, while the effect of fire might not be significant, and found that fire occurrence, along with vegetation community, moisture regime, pH, total carbon, and soil texture are all significant predictors of soil microbial community composition. Burned communities become increasingly dissimilar to unburned communities with increasingly severe burns, and the burn severity index (an index of the fractional area of consumed organic soils and exposed mineral soils) best predicted total bacterial community composition, while whether a site was burned or not was the best predictor for fungi. Globally abundant taxa were identified as significant positive fire responders in this system, includingmore » the bacteria Massilia sp. (64 × more abundant with fire) and Arthrobacter sp. (35×), and the fungi Penicillium sp. (22×) and Fusicladium sp. (12×). Bacterial and fungal co-occurrence network modules were characterized by fire responsiveness as well as pH and moisture regime. Building on the efforts of previous studies, our results consider a particularly wide range of soils, vegetation, and burn severities, and we identify specific fire-responsive microbial taxa and suggest that accounting for burn severity improves our understanding of microbial response to fires.« less

Authors:
ORCiD logo; ; ; ; ;
Publication Date:
Research Org.:
Univ. of California, Berkeley, CA (United States); Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Contributing Org.:
Government of the Northwest Territories; Parks Canada Agency
OSTI Identifier:
1561322
Alternate Identifier(s):
OSTI ID: 1572298
Grant/Contract Number:  
SC0016365
Resource Type:
Published Article
Journal Name:
Soil Biology and Biochemistry
Additional Journal Information:
Journal Name: Soil Biology and Biochemistry Journal Volume: 138 Journal Issue: C; Journal ID: ISSN 0038-0717
Publisher:
Elsevier
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 58 GEOSCIENCES; 54 ENVIRONMENTAL SCIENCES; Wildfire; Burn severity; Boreal; Fungi; Bacteria; Pyrophilic; Fire response

Citation Formats

Whitman, Thea, Whitman, Ellen, Woolet, Jamie, Flannigan, Mike D., Thompson, Dan K., and Parisien, Marc-André. Soil bacterial and fungal response to wildfires in the Canadian boreal forest across a burn severity gradient. United Kingdom: N. p., 2019. Web. doi:10.1016/j.soilbio.2019.107571.
Whitman, Thea, Whitman, Ellen, Woolet, Jamie, Flannigan, Mike D., Thompson, Dan K., & Parisien, Marc-André. Soil bacterial and fungal response to wildfires in the Canadian boreal forest across a burn severity gradient. United Kingdom. https://doi.org/10.1016/j.soilbio.2019.107571
Whitman, Thea, Whitman, Ellen, Woolet, Jamie, Flannigan, Mike D., Thompson, Dan K., and Parisien, Marc-André. Fri . "Soil bacterial and fungal response to wildfires in the Canadian boreal forest across a burn severity gradient". United Kingdom. https://doi.org/10.1016/j.soilbio.2019.107571.
@article{osti_1561322,
title = {Soil bacterial and fungal response to wildfires in the Canadian boreal forest across a burn severity gradient},
author = {Whitman, Thea and Whitman, Ellen and Woolet, Jamie and Flannigan, Mike D. and Thompson, Dan K. and Parisien, Marc-André},
abstractNote = {Global fire regimes are changing, with increases in wildfire frequency and severity expected for many North American forests over the next 100 years. Fires can result in dramatic changes to carbon (C) stocks and can restructure plant and microbial communities, with long-lasting effects on ecosystem functions. We investigated wildfire effects on soil microbial communities (bacteria and fungi) in an extreme fire season in the northwestern Canadian boreal forest, using field surveys, remote sensing, and high-throughput amplicon sequencing in upland and wetland sites. We hypothesized that vegetation community and soil pH would be the most important determinants of microbial community composition, while the effect of fire might not be significant, and found that fire occurrence, along with vegetation community, moisture regime, pH, total carbon, and soil texture are all significant predictors of soil microbial community composition. Burned communities become increasingly dissimilar to unburned communities with increasingly severe burns, and the burn severity index (an index of the fractional area of consumed organic soils and exposed mineral soils) best predicted total bacterial community composition, while whether a site was burned or not was the best predictor for fungi. Globally abundant taxa were identified as significant positive fire responders in this system, including the bacteria Massilia sp. (64 × more abundant with fire) and Arthrobacter sp. (35×), and the fungi Penicillium sp. (22×) and Fusicladium sp. (12×). Bacterial and fungal co-occurrence network modules were characterized by fire responsiveness as well as pH and moisture regime. Building on the efforts of previous studies, our results consider a particularly wide range of soils, vegetation, and burn severities, and we identify specific fire-responsive microbial taxa and suggest that accounting for burn severity improves our understanding of microbial response to fires.},
doi = {10.1016/j.soilbio.2019.107571},
journal = {Soil Biology and Biochemistry},
number = C,
volume = 138,
place = {United Kingdom},
year = {2019},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
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https://doi.org/10.1016/j.soilbio.2019.107571

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Cited by: 6 works
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Works referenced in this record:

Post-Fire Comparisons of Forest Floor and Soil Carbon, Nitrogen, and Mercury Pools with Fire Severity Indices
journal, January 2014


FUNGuild: An open annotation tool for parsing fungal community datasets by ecological guild
journal, April 2016


Microbial interactions: from networks to models
journal, July 2012

  • Faust, Karoline; Raes, Jeroen
  • Nature Reviews Microbiology, Vol. 10, Issue 8
  • DOI: 10.1038/nrmicro2832

rrnDB: improved tools for interpreting rRNA gene abundance in bacteria and archaea and a new foundation for future development
journal, November 2014

  • Stoddard, Steven F.; Smith, Byron J.; Hein, Robert
  • Nucleic Acids Research, Vol. 43, Issue D1
  • DOI: 10.1093/nar/gku1201

Aerobic and anaerobic fungal metabolism and Omics insights for increasing polycyclic aromatic hydrocarbons biodegradation
journal, March 2017

  • Aydin, Sevcan; Karaçay, Hatice Aygün; Shahi, Aiyoub
  • Fungal Biology Reviews, Vol. 31, Issue 2
  • DOI: 10.1016/j.fbr.2016.12.001

The effect of fire on habitat selection of mammalian herbivores: the role of body size and vegetation characteristics
journal, May 2014

  • Eby, Stephanie L.; Anderson, T. Michael; Mayemba, Emilian P.
  • Journal of Animal Ecology, Vol. 83, Issue 5
  • DOI: 10.1111/1365-2656.12221

Functional group diversity increases with modularity in complex food webs
journal, June 2015

  • Montoya, D.; Yallop, M. L.; Memmott, J.
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms8379

Large fires as agents of ecological diversity in the North American boreal forest
journal, January 2008

  • Burton, Philip J.; Parisien, Marc-André; Hicke, Jeffrey A.
  • International Journal of Wildland Fire, Vol. 17, Issue 6
  • DOI: 10.1071/WF07149

Accurate Estimation of Fungal Diversity and Abundance through Improved Lineage-Specific Primers Optimized for Illumina Amplicon Sequencing
journal, October 2016

  • Taylor, D. Lee; Walters, William A.; Lennon, Niall J.
  • Applied and Environmental Microbiology, Vol. 82, Issue 24
  • DOI: 10.1128/AEM.02576-16

Wood bison population recovery and forage availability in northwestern Canada
journal, January 2009


Brefeldin A production by Phoma medicaginis in dead pre-colonized plant tissue: a strategy for habitat conquest?
journal, June 2004


Bacterial composition of soils in ponderosa pine and mixed conifer forests exposed to different wildfire burn severity
journal, February 2014


Fire Reduces Fungal Species Richness and In Situ Mycorrhizal Colonization: A Meta-Analysis
journal, August 2017


Post-fire vegetative dynamics as drivers of microbial community structure and function in forest soils
journal, December 2005

  • Hart, Stephen C.; DeLuca, Thomas H.; Newman, Gregory S.
  • Forest Ecology and Management, Vol. 220, Issue 1-3
  • DOI: 10.1016/j.foreco.2005.08.012

Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform
journal, June 2013

  • Kozich, James J.; Westcott, Sarah L.; Baxter, Nielson T.
  • Applied and Environmental Microbiology, Vol. 79, Issue 17
  • DOI: 10.1128/AEM.01043-13

Potential climate change impacts on fire intensity and key wildfire suppression thresholds in Canada
journal, September 2016

  • Wotton, B. M.; Flannigan, M. D.; Marshall, G. A.
  • Environmental Research Letters, Vol. 12, Issue 9
  • DOI: 10.1088/1748-9326/aa7e6e

Evaluation of the composite burn index for assessing fire severity in Alaskan black spruce forests
journal, January 2008

  • Kasischke, Eric S.; Turetsky, Merritt R.; Ottmar, Roger D.
  • International Journal of Wildland Fire, Vol. 17, Issue 4
  • DOI: 10.1071/WF08002

Testing the ability of visual indicators of soil burn severity to reflect changes in soil chemical and microbial properties in pine forests and shrubland
journal, December 2012


The Sycamore Maple Bacterial Culture Collection From a TNT Polluted Site Shows Novel Plant-Growth Promoting and Explosives Degrading Bacteria
journal, August 2018


Ecologically meaningful transformations for ordination of species data
journal, October 2001


Fire severity effects on soil carbon and nutrients and microbial processes in a Siberian larch forest
journal, October 2018

  • Ludwig, Sarah M.; Alexander, Heather D.; Kielland, Knut
  • Global Change Biology, Vol. 24, Issue 12
  • DOI: 10.1111/gcb.14455

The SILVA and “All-species Living Tree Project (LTP)” taxonomic frameworks
journal, November 2013

  • Yilmaz, Pelin; Parfrey, Laura Wegener; Yarza, Pablo
  • Nucleic Acids Research, Vol. 42, Issue D1
  • DOI: 10.1093/nar/gkt1209

Impacts of climate change on fire activity and fire management in the circumboreal forest
journal, March 2009


The postfire discomycete Geopyxis carbonaria (Ascomycota) is a biotrophic root associate with Norway spruce ( Picea abies ) in nature
journal, May 1998


Wildfire effects on the properties and microbial community structure of organic horizon soils in the New Jersey Pinelands
journal, July 2015


The Systemic Imprint of Growth and Its Uses in Ecological (Meta)Genomics
journal, January 2010


Ecology and evolution of pine life histories
journal, May 2012


Secrets of Soil Survival Revealed by the Genome Sequence of Arthrobacter aurescens TC1
journal, January 2006


A meta-analysis of soil microbial biomass responses to forest disturbances
journal, January 2013


A New Metric for Quantifying Burn Severity: The Relativized Burn Ratio
journal, February 2014

  • Parks, Sean; Dillon, Gregory; Miller, Carol
  • Remote Sensing, Vol. 6, Issue 3
  • DOI: 10.3390/rs6031827

Ectomycorrhizal fungal spore bank recovery after a severe forest fire: some like it hot
journal, October 2015

  • Glassman, Sydney I.; Levine, Carrie R.; DiRocco, Angela M.
  • The ISME Journal, Vol. 10, Issue 5
  • DOI: 10.1038/ismej.2015.182

Degradation of pyrene by immobilized microorganisms in saline-alkaline soil
journal, September 2012


The effect of fire on microbial biomass: a meta-analysis of field studies
journal, August 2011


phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data
journal, April 2013


Using null models to infer microbial co-occurrence networks
journal, May 2017


Variability and drivers of burn severity in the northwestern Canadian boreal forest
journal, February 2018

  • Whitman, Ellen; Parisien, Marc-André; Thompson, Dan K.
  • Ecosphere, Vol. 9, Issue 2
  • DOI: 10.1002/ecs2.2128

Finding community structure in very large networks
journal, December 2004


The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils
journal, July 2017

  • Fernández-González, Antonio J.; Martínez-Hidalgo, Pilar; Cobo-Díaz, José F.
  • Scientific Reports, Vol. 7, Issue 1
  • DOI: 10.1038/s41598-017-06112-3

Arthrobacter chlorophenolicus sp. nov., a new species capable of degrading high concentrations of 4-chlorophenol
journal, November 2000

  • Westerberg, K.; Elvang, A. M.; Stackebrandt, E.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 50, Issue 6
  • DOI: 10.1099/00207713-50-6-2083

Nitrogen alters carbon dynamics during early succession in boreal forest
journal, July 2010


Molecular ecological network analyses
journal, January 2012


Mapping fire extent and burn severity in Alaskan tussock tundra: An analysis of the spectral response of tundra vegetation to wildland fire
journal, July 2013


The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012

  • Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
  • Nucleic Acids Research, Vol. 41, Issue D1
  • DOI: 10.1093/nar/gks1219

Experimental warming and burn severity alter soil CO2 flux and soil functional groups in a recently burned boreal forest
journal, December 2004


Climate change impacts on future boreal fire regimes
journal, April 2013


Vegetation and wildland fire: Implications of global climate change
journal, January 1991


Metagenomic Assessment of the Potential Microbial Nitrogen Pathways in the Rhizosphere of a Mediterranean Forest After a Wildfire
journal, March 2015

  • Cobo-Díaz, José F.; Fernández-González, Antonio J.; Villadas, Pablo J.
  • Microbial Ecology, Vol. 69, Issue 4
  • DOI: 10.1007/s00248-015-0586-7

De Quelques Espèces Nouvelles De Champignons
journal, January 1877


Revision of “Septonema ochraceum” revealed three new species of Venturiaceae and Herpotrichiellaceae
journal, December 2009


Global diversity and geography of soil fungi
journal, November 2014


Inferring changes in soil organic matter in post-wildfire soil burn severity levels in a temperate climate
journal, June 2018


Macroinvertebrate and soil prokaryote communities in the forest–tundra ecotone of the Subarctic Yukon
journal, May 2018


Neurospora in temperate forests of western North America
journal, January 2004


Relic DNA is abundant in soil and obscures estimates of soil microbial diversity
journal, December 2016


Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
journal, October 2009

  • Schloss, P. D.; Westcott, S. L.; Ryabin, T.
  • Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541
  • DOI: 10.1128/AEM.01541-09

Arthrobacter siccitolerans sp. nov., a highly desiccation-tolerant, xeroprotectant-producing strain isolated from dry soil
journal, June 2013

  • SantaCruz-Calvo, L.; Gonzalez-Lopez, J.; Manzanera, M.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 63, Issue Pt 11
  • DOI: 10.1099/ijs.0.052902-0

QIIME allows analysis of high-throughput community sequencing data
journal, April 2010

  • Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
  • Nature Methods, Vol. 7, Issue 5
  • DOI: 10.1038/nmeth.f.303

Changes in assembly processes in soil bacterial communities following a wildfire disturbance
journal, February 2013

  • Ferrenberg, Scott; O'Neill, Sean P.; Knelman, Joseph E.
  • The ISME Journal, Vol. 7, Issue 6
  • DOI: 10.1038/ismej.2013.11

Trichocomaceae in bark survive high temperatures and fire: Trichocomaceae in bark survive high temperatures and fire
journal, June 2006


DADA2: High-resolution sample inference from Illumina amplicon data
journal, May 2016

  • Callahan, Benjamin J.; McMurdie, Paul J.; Rosen, Michael J.
  • Nature Methods, Vol. 13, Issue 7
  • DOI: 10.1038/nmeth.3869

Differential abundance analysis for microbial marker-gene surveys
journal, September 2013

  • Paulson, Joseph N.; Stine, O. Colin; Bravo, Héctor Corrada
  • Nature Methods, Vol. 10, Issue 12
  • DOI: 10.1038/nmeth.2658

PEAR: a fast and accurate Illumina Paired-End reAd mergeR
journal, October 2013


Global estimates of boreal forest carbon stocks and flux
journal, May 2015


Fire severity influences the response of soil microbes to a boreal forest fire
journal, February 2016

  • Holden, Sandra R.; Rogers, Brendan M.; Treseder, Kathleen K.
  • Environmental Research Letters, Vol. 11, Issue 3
  • DOI: 10.1088/1748-9326/11/3/035004

rRNA Operon Copy Number Reflects Ecological Strategies of Bacteria
journal, April 2000


Modularity and community structure in networks
journal, May 2006

  • Newman, M. E. J.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 23
  • DOI: 10.1073/pnas.0601602103

The diversity and biogeography of soil bacterial communities
journal, January 2006

  • Fierer, N.; Jackson, R. B.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 3
  • DOI: 10.1073/pnas.0507535103

The modularity of pollination networks
journal, December 2007

  • Olesen, J. M.; Bascompte, J.; Dupont, Y. L.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 50
  • DOI: 10.1073/pnas.0706375104

A global atlas of the dominant bacteria found in soil
journal, January 2018

  • Delgado-Baquerizo, Manuel; Oliverio, Angela M.; Brewer, Tess E.
  • Science, Vol. 359, Issue 6373
  • DOI: 10.1126/science.aap9516

Fire as the dominant driver of central Canadian boreal forest carbon balance
journal, November 2007

  • Bond-Lamberty, Ben; Peckham, Scott D.; Ahl, Douglas E.
  • Nature, Vol. 450, Issue 7166
  • DOI: 10.1038/nature06272

Pyroaerobiology: the aerosolization and transport of viable microbial life by wildland fire
journal, November 2018

  • Kobziar, Leda N.; Pingree, Melissa R. A.; Larson, Heather
  • Ecosphere, Vol. 9, Issue 11
  • DOI: 10.1002/ecs2.2507

The interconnected rhizosphere: High network complexity dominates rhizosphere assemblages
journal, June 2016

  • Shi, Shengjing; Nuccio, Erin E.; Shi, Zhou J.
  • Ecology Letters, Vol. 19, Issue 8
  • DOI: 10.1111/ele.12630

An alternative fire regime zonation for Canada
journal, January 2012

  • Boulanger, Yan; Gauthier, Sylvie; Burton, Philip. J.
  • International Journal of Wildland Fire, Vol. 21, Issue 8
  • DOI: 10.1071/WF11073

Large forest fires in Canada, 1959–1997
journal, December 2002

  • Stocks, B. J.; Mason, J. A.; Todd, J. B.
  • Journal of Geophysical Research, Vol. 108, Issue D1
  • DOI: 10.1029/2001JD000484

An introduction to Canada’s boreal zone: ecosystem processes, health, sustainability, and environmental issues
journal, December 2013

  • Brandt, J. P.; Flannigan, M. D.; Maynard, D. G.
  • Environmental Reviews, Vol. 21, Issue 4
  • DOI: 10.1139/er-2013-0040

The genus Fimetariella
journal, December 1995

  • Krug, John C.
  • Canadian Journal of Botany, Vol. 73, Issue 12
  • DOI: 10.1139/b95-203

Functional cartography of complex metabolic networks
journal, February 2005

  • Guimerà, Roger; Nunes Amaral, Luís A.
  • Nature, Vol. 433, Issue 7028
  • DOI: 10.1038/nature03288

Functional Molecular Ecological Networks
journal, October 2010