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Title: Convenient non-invasive electrochemical techniques to monitor microbial processes: current state and perspectives

Abstract

Real-time electrochemical monitoring in bioprocesses is an improvement over existing systems because it is versatile and provides more information to the user than periodic measurements of cell density or metabolic activity. Real-time electrochemical monitoring provides the ability to monitor the physiological status of actively growing cells related to electron transfer activity and potential changes in the proton gradient of the cells. Voltammetric and amperometric techniques offer opportunities to monitor electron transfer reactions when electrogenic microbes are used in microbial fuel cells or bioelectrochemical synthesis. Impedance techniques provide the ability to monitor the physiological status of a wide range of microorganisms in conventional bioprocesses. Impedance techniques involve scanning a range of frequencies to define physiological activity in terms of equivalent electrical circuits, thereby enabling the use of computer modeling to evaluate specific growth parameters. Electrochemical monitoring of microbial activity has applications throughout the biotechnology industry for generating real-time data and offers the potential for automated process controls for specific bioprocesses.

Authors:
ORCiD logo [1];  [2];  [2];  [2];  [3]
  1. Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)
  2. Univ. of South Carolina, Columbia, SC (United States)
  3. Savannah River Consulting, Aiken, SC (United States)
Publication Date:
Research Org.:
Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Laboratory (SRNL)
Sponsoring Org.:
USDOE Office of Environmental Management (EM)
OSTI Identifier:
1561215
Report Number(s):
SRNL-STI-2019-00550
Journal ID: ISSN 0175-7598
Grant/Contract Number:  
AC09-08SR22470
Resource Type:
Accepted Manuscript
Journal Name:
Applied Microbiology and Biotechnology
Additional Journal Information:
Journal Name: Applied Microbiology and Biotechnology; Journal ID: ISSN 0175-7598
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bioprocess; in situ monitoring; cyclic voltammetry; electrochemical impedance spectroscopy; microbial fuel cell; bioelectrosynthesis

Citation Formats

Turick, Charles E., Shimpalee, Sirivatch, Satjaritanun, Pongsarun, Weidner, John, and Greenway, Scott. Convenient non-invasive electrochemical techniques to monitor microbial processes: current state and perspectives. United States: N. p., 2019. Web. doi:10.1007/s00253-019-10091-y.
Turick, Charles E., Shimpalee, Sirivatch, Satjaritanun, Pongsarun, Weidner, John, & Greenway, Scott. Convenient non-invasive electrochemical techniques to monitor microbial processes: current state and perspectives. United States. doi:10.1007/s00253-019-10091-y.
Turick, Charles E., Shimpalee, Sirivatch, Satjaritanun, Pongsarun, Weidner, John, and Greenway, Scott. Wed . "Convenient non-invasive electrochemical techniques to monitor microbial processes: current state and perspectives". United States. doi:10.1007/s00253-019-10091-y. https://www.osti.gov/servlets/purl/1561215.
@article{osti_1561215,
title = {Convenient non-invasive electrochemical techniques to monitor microbial processes: current state and perspectives},
author = {Turick, Charles E. and Shimpalee, Sirivatch and Satjaritanun, Pongsarun and Weidner, John and Greenway, Scott},
abstractNote = {Real-time electrochemical monitoring in bioprocesses is an improvement over existing systems because it is versatile and provides more information to the user than periodic measurements of cell density or metabolic activity. Real-time electrochemical monitoring provides the ability to monitor the physiological status of actively growing cells related to electron transfer activity and potential changes in the proton gradient of the cells. Voltammetric and amperometric techniques offer opportunities to monitor electron transfer reactions when electrogenic microbes are used in microbial fuel cells or bioelectrochemical synthesis. Impedance techniques provide the ability to monitor the physiological status of a wide range of microorganisms in conventional bioprocesses. Impedance techniques involve scanning a range of frequencies to define physiological activity in terms of equivalent electrical circuits, thereby enabling the use of computer modeling to evaluate specific growth parameters. Electrochemical monitoring of microbial activity has applications throughout the biotechnology industry for generating real-time data and offers the potential for automated process controls for specific bioprocesses.},
doi = {10.1007/s00253-019-10091-y},
journal = {Applied Microbiology and Biotechnology},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {8}
}

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