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Title: Epigenetic silencing of a multifunctional plant stress regulator

Journal Article · · eLife
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [2]; ORCiD logo [1];  [3];  [1]; ORCiD logo [4];  [1];  [1];  [1]; ORCiD logo [1];  [1]; ORCiD logo [5];  [6]; ORCiD logo [7]; ORCiD logo [8];  [1]; ORCiD logo [1]
  1. Salk Institute for Biological Studies, San Diego, CA (United States)
  2. Salk Institute for Biological Studies, San Diego, CA (United States); Illumina, San Diego, CA (United States)
  3. Salk Institute for Biological Studies, San Diego, CA (United States); NAVICAN, San Diego, CA (United States)
  4. Univ. of Pennsylvania, Philadelphia, PA (United States); Drexel Univ., Philadelphia, PA (United States)
  5. Salk Institute for Biological Studies, San Diego, CA (United States); Bayer Crop Science, Chesterfield, MO (United Stated)
  6. Salk Institute for Biological Studies, San Diego, CA (United States); North Carolina State Univ., Raleigh, NC (United States)
  7. Salk Institute for Biological Studies, San Diego, CA (United States); Univ. of Georgia, Athens, GA (United States)
  8. Salk Institute for Biological Studies, San Diego, CA (United States); La Trobe Univ., Melbourne, VIC (Australia)

The central regulator of the ethylene (ET) signaling pathway, which controls a plethora of developmental programs and responses to environmental cues in plants, is ETHYLENE-INSENSITIVE2 (EIN2). Here we identify a chromatin-dependent regulatory mechanism at EIN2 requiring two genes: ETHYLENE-INSENSITIVE6 (EIN6), which is a H3K27me3 demethylase also known as RELATIVE OF EARLY FLOWERING6 (REF6), and EIN6 ENHANCER (EEN), the Arabidopsis homolog of the yeast INO80 chromatin remodeling complex subunit IES6 (INO EIGHTY SUBUNIT). Strikingly, EIN6 (REF6) and the INO80 complex redundantly control the level and the localization of the repressive histone modification H3K27me3 and the histone variant H2A.Z at the 5’ untranslated region (5’UTR) intron of EIN2. Concomitant loss of EIN6 (REF6) and the INO80 complex shifts the chromatin landscape at EIN2 to a repressive state causing a dramatic reduction of EIN2 expression. These results uncover a unique type of chromatin regulation which safeguards the expression of an essential multifunctional plant stress regulator.

Research Organization:
Salk Institute for Biological Studies, La Jolla, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC); National Institutes of Health (NIH)
Grant/Contract Number:
FG02-04ER15517
OSTI ID:
1560888
Alternate ID(s):
OSTI ID: 1560889; OSTI ID: 1609505
Journal Information:
eLife, Vol. 8; ISSN 2050-084X
Publisher:
eLife Sciences Publications, Ltd.Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 24 works
Citation information provided by
Web of Science

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