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Title: Experimental warming alters the community composition, diversity, and N 2 fixation activity of peat moss ( Sphagnum fallax ) microbiomes

Abstract

SphagnumSphagnum are known to harbor diverse microbial communities that mediate C and nitrogen (N) cycling in peatlands, the effects of climate change on Sphagnum microbiome composition and functioning are largely unknown. We investigated the impacts of experimental whole-ecosystem warming on the Sphagnum moss microbiome, focusing on N 2 fixing microorganisms (diazotrophs). To characterize the microbiome response to warming, we performed next-generation sequencing of small subunit (SSU) rRNA and nitrogenase ( niƒH) gene amplicons and quantified rates of N 2 fixation activity in Sphagnum fallax2 fixation rates, decreased with warming ( p < 0.05). Following warming, diazotrophs shifted from a mixed community of Nostocales (Cyanobacteria) and Rhizobiales (Alphaproteobacteria) to predominance of Nostocales. Microbiome community composition differed between years, with some diazotroph populations persisting while others declined in relative abundance in warmed plots in the second year. Our results demonstrate that warming substantially alters the community composition, diversity, and N 2 fixation activity of peat moss microbiomes, which may ultimately impact host fitness, ecosystem productivity, and C storage potential in peatlands.

Authors:
ORCiD logo [1];  [2]; ORCiD logo [3];  [4];  [3];  [5];  [6];  [6]; ORCiD logo [7]
  1. Bredesen Center for Interdisciplinary Research and Graduate Education University of Tennessee Knoxville Tennessee, Biosciences Division Oak Ridge National Laboratory Oak Ridge Tennessee
  2. School of Biology Georgia Institute of Technology Atlanta Georgia
  3. School of Earth and Atmospheric Sciences Georgia Institute of Technology Atlanta Georgia
  4. Biosciences Division Oak Ridge National Laboratory Oak Ridge Tennessee
  5. School of Biology Georgia Institute of Technology Atlanta Georgia, School of Earth and Atmospheric Sciences Georgia Institute of Technology Atlanta Georgia
  6. Environmental Sciences Division Oak Ridge National Laboratory Oak Ridge Tennessee, Climate Change Science Institute, Oak Ridge National Laboratory Oak Ridge Tennessee
  7. Biosciences Division Oak Ridge National Laboratory Oak Ridge Tennessee, Climate Change Science Institute, Oak Ridge National Laboratory Oak Ridge Tennessee
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1560689
Alternate Identifier(s):
OSTI ID: 1559747; OSTI ID: 1560690
Grant/Contract Number:  
AC05-00OR22725; SC0007144; SC0012088
Resource Type:
Published Article
Journal Name:
Global Change Biology
Additional Journal Information:
Journal Name: Global Change Biology Journal Volume: 25 Journal Issue: 9; Journal ID: ISSN 1354-1013
Publisher:
Wiley
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Carrell, Alyssa A., Kolton, Max, Glass, Jennifer B., Pelletier, Dale A., Warren, Melissa J., Kostka, Joel E., Iversen, Colleen M., Hanson, Paul J., and Weston, David J. Experimental warming alters the community composition, diversity, and N 2 fixation activity of peat moss ( Sphagnum fallax ) microbiomes. United Kingdom: N. p., 2019. Web. doi:10.1111/gcb.14715.
Carrell, Alyssa A., Kolton, Max, Glass, Jennifer B., Pelletier, Dale A., Warren, Melissa J., Kostka, Joel E., Iversen, Colleen M., Hanson, Paul J., & Weston, David J. Experimental warming alters the community composition, diversity, and N 2 fixation activity of peat moss ( Sphagnum fallax ) microbiomes. United Kingdom. doi:10.1111/gcb.14715.
Carrell, Alyssa A., Kolton, Max, Glass, Jennifer B., Pelletier, Dale A., Warren, Melissa J., Kostka, Joel E., Iversen, Colleen M., Hanson, Paul J., and Weston, David J. Mon . "Experimental warming alters the community composition, diversity, and N 2 fixation activity of peat moss ( Sphagnum fallax ) microbiomes". United Kingdom. doi:10.1111/gcb.14715.
@article{osti_1560689,
title = {Experimental warming alters the community composition, diversity, and N 2 fixation activity of peat moss ( Sphagnum fallax ) microbiomes},
author = {Carrell, Alyssa A. and Kolton, Max and Glass, Jennifer B. and Pelletier, Dale A. and Warren, Melissa J. and Kostka, Joel E. and Iversen, Colleen M. and Hanson, Paul J. and Weston, David J.},
abstractNote = {SphagnumSphagnum are known to harbor diverse microbial communities that mediate C and nitrogen (N) cycling in peatlands, the effects of climate change on Sphagnum microbiome composition and functioning are largely unknown. We investigated the impacts of experimental whole-ecosystem warming on the Sphagnum moss microbiome, focusing on N2 fixing microorganisms (diazotrophs). To characterize the microbiome response to warming, we performed next-generation sequencing of small subunit (SSU) rRNA and nitrogenase (niƒH) gene amplicons and quantified rates of N2 fixation activity in Sphagnum fallax2 fixation rates, decreased with warming (p < 0.05). Following warming, diazotrophs shifted from a mixed community of Nostocales (Cyanobacteria) and Rhizobiales (Alphaproteobacteria) to predominance of Nostocales. Microbiome community composition differed between years, with some diazotroph populations persisting while others declined in relative abundance in warmed plots in the second year. Our results demonstrate that warming substantially alters the community composition, diversity, and N2 fixation activity of peat moss microbiomes, which may ultimately impact host fitness, ecosystem productivity, and C storage potential in peatlands.},
doi = {10.1111/gcb.14715},
journal = {Global Change Biology},
number = 9,
volume = 25,
place = {United Kingdom},
year = {2019},
month = {5}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/gcb.14715

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