Reversal of carbapenem-resistance in Shewanella algae by CRISPR/Cas9 genome editing

Journal Article · 30 January 2019 · Journal of Advanced Research

DOI: https://doi.org/10.1016/j.jare.2019.01.011 · OSTI ID:1560601

Wu, Zong-Yen ^[1]; Huang, Yao-Ting ^[2]; Chao, Wen-Cheng ^[3]; Ho, Shu-Peng ^[4]; Cheng, Jan-Fang ^[5]; Liu, Po-Yu  ^[6]

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1. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); National Chung Hsing Univ., Taichung (Taiwan)
2. National Chung Cheng Univ., Chia-Yi (Taiwan)
3. Taichung Veterans General Hospital, Taichung (Taiwan)
4. National Chung Hsing Univ., Taichung (Taiwan)
5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
6. Taichung Veterans General Hospital, Taichung (Taiwan); National Chung Hsing Univ., Taichung (Taiwan); Shu-Zen Junior College of Medicine and Management, Kaohsiung City (Taiwan); National Chung Hsing Univ., Taichung (Taiwan)

Antibiotic resistance in pathogens is a growing threat to human health. Of particular concern is resistance to carbapenem, which is an antimicrobial agent listed as critically important by the World Health Organization. With the global spread of carbapenem-resistant organisms, there is an urgent need for new treatment options. Shewanella algae is an emerging pathogen found in marine environments throughout the world that has increasing resistance to carbapenem. The organism is also a possible antibiotic resistance reservoir in humans and in its natural habitat. The development of CRISPR/Cas9-based methods has enabled precise genetic manipulation. A number of attempts have been made to knock out resistance genes in various organisms. The study used a single plasmid containing CRISPR/Cas9 and recE/recT recombinase to reverse an antibiotic-resistant phenotype in S. algae and showed bla _OXA-55-like gene is essential for the carbapenem resistance. This result demonstrates a potential validation strategy for functional genome annotation in S. algae.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• Grant/Contract Number: AC02-05CH11231
• OSTI ID: 1560601
• Journal Information: Journal of Advanced Research, Journal Name: Journal of Advanced Research Journal Issue: C Vol. 18; ISSN 2090-1232
• Publisher: ElsevierCopyright Statement
• Country of Publication: United States
• Language: English