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Title: Cytolytic Perforin as an Adjuvant to Enhance the Immunogenicity of DNA Vaccines

Abstract

DNA vaccines present one of the most cost-effective platforms to develop global vaccines, which have been tested for nearly three decades in preclinical and clinical settings with some success in the clinic. However, one of the major challenges for the development of DNA vaccines is their poor immunogenicity in humans, which has led to refinements in DNA delivery, dosage in prime/boost regimens and the inclusion of adjuvants to enhance their immunogenicity. In this review, we focus on adjuvants that can enhance the immunogenicity of DNA encoded antigens and highlight the development of a novel cytolytic DNA platform encoding a truncated mouse perforin. The application of this innovative DNA technology has considerable potential in the development of effective vaccines.

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of Adelaide, SA (Australia). Virology Lab., Discipline of Surgery, Basil Hetzel Inst. for Translational Health Research
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Health and Medical Research Council; Australian Centre for HIV and Hepatitis Virology Research; the National Foundation for Medical Research and Innovation; The Hospital Research Foundation (THRF)
OSTI Identifier:
1560594
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Vaccines
Additional Journal Information:
Journal Volume: 7; Journal Issue: 2; Journal ID: ISSN 2076-393X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; DNA vaccine; adjuvants; vaccine delivery; plasmid; cytolytic; perforin; bicistronic; HCV; HIV

Citation Formats

Shrestha, Ashish C., Wijesundara, Danushka K., Masavuli, Makutiro G., Mekonnen, Zelalem A., Gowans, Eric J., and Grubor-Bauk, Branka. Cytolytic Perforin as an Adjuvant to Enhance the Immunogenicity of DNA Vaccines. United States: N. p., 2019. Web. doi:10.3390/vaccines7020038.
Shrestha, Ashish C., Wijesundara, Danushka K., Masavuli, Makutiro G., Mekonnen, Zelalem A., Gowans, Eric J., & Grubor-Bauk, Branka. Cytolytic Perforin as an Adjuvant to Enhance the Immunogenicity of DNA Vaccines. United States. doi:10.3390/vaccines7020038.
Shrestha, Ashish C., Wijesundara, Danushka K., Masavuli, Makutiro G., Mekonnen, Zelalem A., Gowans, Eric J., and Grubor-Bauk, Branka. Tue . "Cytolytic Perforin as an Adjuvant to Enhance the Immunogenicity of DNA Vaccines". United States. doi:10.3390/vaccines7020038. https://www.osti.gov/servlets/purl/1560594.
@article{osti_1560594,
title = {Cytolytic Perforin as an Adjuvant to Enhance the Immunogenicity of DNA Vaccines},
author = {Shrestha, Ashish C. and Wijesundara, Danushka K. and Masavuli, Makutiro G. and Mekonnen, Zelalem A. and Gowans, Eric J. and Grubor-Bauk, Branka},
abstractNote = {DNA vaccines present one of the most cost-effective platforms to develop global vaccines, which have been tested for nearly three decades in preclinical and clinical settings with some success in the clinic. However, one of the major challenges for the development of DNA vaccines is their poor immunogenicity in humans, which has led to refinements in DNA delivery, dosage in prime/boost regimens and the inclusion of adjuvants to enhance their immunogenicity. In this review, we focus on adjuvants that can enhance the immunogenicity of DNA encoded antigens and highlight the development of a novel cytolytic DNA platform encoding a truncated mouse perforin. The application of this innovative DNA technology has considerable potential in the development of effective vaccines.},
doi = {10.3390/vaccines7020038},
journal = {Vaccines},
number = 2,
volume = 7,
place = {United States},
year = {2019},
month = {4}
}

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