skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Signals Getting Crossed in the Entanglement of Redox and Phosphorylation Pathways: Phosphorylation of Peroxiredoxin Proteins Sparks Cell Signaling

Abstract

Reactive oxygen and nitrogen species have cell signaling properties and are involved in a multitude of processes beyond redox homeostasis. The peroxiredoxin (Prdx) proteins are highly sensitive intracellular peroxidases that can coordinate cell signaling via direct reactive species scavenging or by acting as a redox sensor that enables control of binding partner activity. Oxidation of the peroxidatic cysteine residue of Prdx proteins are the classical post-translational modification that has been recognized to modulate downstream signaling cascades, but increasing evidence supports that dynamic changes to phosphorylation of Prdx proteins is also an important determinant in redox signaling. Phosphorylation of Prdx proteins affects three-dimensional structure and function to coordinate cell proliferation, wound healing, cell fate and lipid signaling. The advent of large proteomic datasets has shown that there are many opportunities to understand further how phosphorylation of Prdx proteins fit into intracellular signaling cascades in normal or malignant cells and that more research is necessary. This review summarizes the Prdx family of proteins and details how post-translational modification by kinases and phosphatases controls intracellular signaling.

Authors:
 [1];  [1];  [1]
  1. Univ. of Pittsburgh, Pittsburgh, PA (United States). Dept. of Pharmacology and Chemical Biology, School of Medicine; Univ. of Pittsburgh, Pittsburgh, PA (United States). Medical Center Hillman Cancer Center, Women’s Cancer Research Center; Magee-Women’s Hospital of UPMC, Pittsburgh, PA (United States). Magee-Women’s Research Inst.
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1560587
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Antioxidants
Additional Journal Information:
Journal Volume: 8; Journal Issue: 2; Journal ID: ISSN 2076-3921
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; peroxiredoxin; Prdx; Prx; redox signaling; cell signaling; phosphorylation

Citation Formats

Skoko, John, Attaran, Shireen, and Neumann, Carola. Signals Getting Crossed in the Entanglement of Redox and Phosphorylation Pathways: Phosphorylation of Peroxiredoxin Proteins Sparks Cell Signaling. United States: N. p., 2019. Web. doi:10.3390/antiox8020029.
Skoko, John, Attaran, Shireen, & Neumann, Carola. Signals Getting Crossed in the Entanglement of Redox and Phosphorylation Pathways: Phosphorylation of Peroxiredoxin Proteins Sparks Cell Signaling. United States. doi:10.3390/antiox8020029.
Skoko, John, Attaran, Shireen, and Neumann, Carola. Wed . "Signals Getting Crossed in the Entanglement of Redox and Phosphorylation Pathways: Phosphorylation of Peroxiredoxin Proteins Sparks Cell Signaling". United States. doi:10.3390/antiox8020029. https://www.osti.gov/servlets/purl/1560587.
@article{osti_1560587,
title = {Signals Getting Crossed in the Entanglement of Redox and Phosphorylation Pathways: Phosphorylation of Peroxiredoxin Proteins Sparks Cell Signaling},
author = {Skoko, John and Attaran, Shireen and Neumann, Carola},
abstractNote = {Reactive oxygen and nitrogen species have cell signaling properties and are involved in a multitude of processes beyond redox homeostasis. The peroxiredoxin (Prdx) proteins are highly sensitive intracellular peroxidases that can coordinate cell signaling via direct reactive species scavenging or by acting as a redox sensor that enables control of binding partner activity. Oxidation of the peroxidatic cysteine residue of Prdx proteins are the classical post-translational modification that has been recognized to modulate downstream signaling cascades, but increasing evidence supports that dynamic changes to phosphorylation of Prdx proteins is also an important determinant in redox signaling. Phosphorylation of Prdx proteins affects three-dimensional structure and function to coordinate cell proliferation, wound healing, cell fate and lipid signaling. The advent of large proteomic datasets has shown that there are many opportunities to understand further how phosphorylation of Prdx proteins fit into intracellular signaling cascades in normal or malignant cells and that more research is necessary. This review summarizes the Prdx family of proteins and details how post-translational modification by kinases and phosphatases controls intracellular signaling.},
doi = {10.3390/antiox8020029},
journal = {Antioxidants},
number = 2,
volume = 8,
place = {United States},
year = {2019},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 2 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Basic Principles and Emerging Concepts in the Redox Control of Transcription Factors
journal, October 2011

  • Brigelius-Flohé, Regina; Flohé, Leopold
  • Antioxidants & Redox Signaling, Vol. 15, Issue 8
  • DOI: 10.1089/ars.2010.3534

Peroxiredoxin 6: A Bifunctional Enzyme with Glutathione Peroxidase and Phospholipase A 2 Activities
journal, August 2011


Peroxiredoxin Involvement in the Initiation and Progression of Human Cancer
journal, March 2018

  • Hampton, Mark B.; Vick, Kate A.; Skoko, John J.
  • Antioxidants & Redox Signaling, Vol. 28, Issue 7
  • DOI: 10.1089/ars.2017.7422

Peroxiredoxin Evolution and the Regulation of Hydrogen Peroxide Signaling
journal, April 2003


Regulation of Peroxiredoxin I Activity by Cdc2-mediated Phosphorylation
journal, May 2002

  • Chang, Tong-Shin; Jeong, Woojin; Choi, Soon Young
  • Journal of Biological Chemistry, Vol. 277, Issue 28
  • DOI: 10.1074/jbc.M110432200

The c-Myc target gene PRDX3 is required for mitochondrial homeostasis and neoplastic transformation
journal, May 2002

  • Wonsey, D. R.; Zeller, K. I.; Dang, C. V.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 10
  • DOI: 10.1073/pnas.102523299

Phosphosignature Predicts Dasatinib Response in Non-small Cell Lung Cancer
journal, May 2012

  • Klammer, Martin; Kaminski, Marc; Zedler, Alexandra
  • Molecular & Cellular Proteomics, Vol. 11, Issue 9
  • DOI: 10.1074/mcp.M111.016410

Glutathionylation of the Active Site Cysteines of Peroxiredoxin 2 and Recycling by Glutaredoxin
journal, November 2015

  • Peskin, Alexander V.; Pace, Paul E.; Behring, Jessica B.
  • Journal of Biological Chemistry, Vol. 291, Issue 6
  • DOI: 10.1074/jbc.M115.692798

How mitochondria produce reactive oxygen species
journal, December 2008


Irreversible Oxidation of the Active-site Cysteine of Peroxiredoxin to Cysteine Sulfonic Acid for Enhanced Molecular Chaperone Activity
journal, August 2008

  • Lim, Jung Chae; Choi, Hoon-In; Park, Yu Sun
  • Journal of Biological Chemistry, Vol. 283, Issue 43
  • DOI: 10.1074/jbc.M804087200

The cellular production of hydrogen peroxide
journal, July 1972

  • Boveris, Alberto; Oshino, Nozomu; Chance, Britton
  • Biochemical Journal, Vol. 128, Issue 3
  • DOI: 10.1042/bj1280617

Crystal structure of human peroxiredoxin 5, a novel type of mammalian peroxiredoxin at 1.5 Å resolution
journal, August 2001

  • Declercq, Jean-Paul; Evrard, Christine; Clippe, André
  • Journal of Molecular Biology, Vol. 311, Issue 4
  • DOI: 10.1006/jmbi.2001.4853

Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry
journal, February 2007

  • Molina, H.; Horn, D. M.; Tang, N.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 7
  • DOI: 10.1073/pnas.0611217104

Control of the pericentrosomal H 2 O 2 level by peroxiredoxin I is critical for mitotic progression
journal, July 2015

  • Lim, Jung Mi; Lee, Kyung S.; Woo, Hyun Ae
  • The Journal of Cell Biology, Vol. 210, Issue 1
  • DOI: 10.1083/jcb.201412068

Survey of Tyrosine Kinase Signaling Reveals ROS Kinase Fusions in Human Cholangiocarcinoma
journal, January 2011


Multiomic Analysis of the UV-Induced DNA Damage Response
journal, May 2016


Quantitative phosphoproteomic analysis of IL-33-mediated signaling
journal, January 2015

  • Pinto, Sneha M.; Nirujogi, Raja Sekhar; Rojas, Pamela Leal
  • PROTEOMICS, Vol. 15, Issue 2-3
  • DOI: 10.1002/pmic.201400303

Reactive Oxygen Species: A Key Constituent in Cancer Survival
journal, January 2018


Quantitative phosphoproteomics of vasopressin-sensitive renal cells: Regulation of aquaporin-2 phosphorylation at two sites
journal, April 2006

  • Hoffert, J. D.; Pisitkun, T.; Wang, G.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 18
  • DOI: 10.1073/pnas.0600895103

Mammalian catalase: a venerable enzyme with new mysteries
journal, January 2007


Ischemia in Tumors Induces Early and Sustained Phosphorylation Changes in Stress Kinase Pathways but Does Not Affect Global Protein Levels
journal, April 2014

  • Mertins, Philipp; Yang, Feng; Liu, Tao
  • Molecular & Cellular Proteomics, Vol. 13, Issue 7
  • DOI: 10.1074/mcp.M113.036392

Integrated proteomic analysis of post-translational modifications by serial enrichment
journal, June 2013

  • Mertins, Philipp; Qiao, Jana W.; Patel, Jinal
  • Nature Methods, Vol. 10, Issue 7
  • DOI: 10.1038/nmeth.2518

Regulation of extinction of cocaine-induced place preference by midkine is related to a differential phosphorylation of peroxiredoxin 6 in dorsal striatum
journal, September 2013

  • Gramage, Esther; Pérez-García, Carmen; Vicente-Rodríguez, Marta
  • Behavioural Brain Research, Vol. 253
  • DOI: 10.1016/j.bbr.2013.07.026

Investigation of Receptor interacting protein (RIP3)-dependent Protein Phosphorylation by Quantitative Phosphoproteomics
journal, August 2012


The High Reactivity of Peroxiredoxin 2 with H 2 O 2 Is Not Reflected in Its Reaction with Other Oxidants and Thiol Reagents
journal, February 2007

  • Peskin, Alexander V.; Low, Felicia M.; Paton, Louise N.
  • Journal of Biological Chemistry, Vol. 282, Issue 16
  • DOI: 10.1074/jbc.M700339200

Caspase cleavage of MST1 promotes nuclear translocation and chromatin condensation
journal, August 2001

  • Ura, S.; Masuyama, N.; Graves, J. D.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 18
  • DOI: 10.1073/pnas.181161698

Role of Cdk5-Mediated Phosphorylation of Prx2 in MPTP Toxicity and Parkinson's Disease
journal, July 2007


Glutathione peroxidases
journal, May 2013

  • Brigelius-Flohé, Regina; Maiorino, Matilde
  • Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1830, Issue 5
  • DOI: 10.1016/j.bbagen.2012.11.020

Production of H 2 O 2 in the Endoplasmic Reticulum Promotes In Vivo Disulfide Bond Formation
journal, May 2012

  • Margittai, Éva; Löw, Péter; Stiller, Ibolya
  • Antioxidants & Redox Signaling, Vol. 16, Issue 10
  • DOI: 10.1089/ars.2011.4221

Spatial Phosphoprotein Profiling Reveals a Compartmentalized Extracellular Signal-regulated Kinase Switch Governing Neurite Growth and Retraction
journal, March 2011

  • Wang, Yingchun; Yang, Feng; Fu, Yi
  • Journal of Biological Chemistry, Vol. 286, Issue 20
  • DOI: 10.1074/jbc.M111.236133

Mislocalized Activation of Oncogenic RTKs Switches Downstream Signaling Outcomes
journal, October 2009


A Quantitative Map of the Liver Mitochondrial Phosphoproteome Reveals Posttranslational Control of Ketogenesis
journal, November 2012


Proteomic profiling of differentially expressed proteins from Bax inhibitor-1 knockout and wild type mice
journal, June 2012


Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology
journal, January 2017


A Tissue-Specific Atlas of Mouse Protein Phosphorylation and Expression
journal, December 2010


The Tumor Suppressor Mst1 Promotes Changes in the Cellular Redox State by Phosphorylation and Inactivation of Peroxiredoxin-1 Protein
journal, February 2013

  • Rawat, Sonali Jalan; Creasy, Caretha L.; Peterson, Jeffrey R.
  • Journal of Biological Chemistry, Vol. 288, Issue 12
  • DOI: 10.1074/jbc.M112.414524

Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors
journal, October 2013


Mitochondrial oxidative stress in mice lacking the glutathione peroxidase-1 gene
journal, March 2000

  • Esposito, Luke A.; Kokoszka, Jason E.; Waymire, Katrina G.
  • Free Radical Biology and Medicine, Vol. 28, Issue 5
  • DOI: 10.1016/S0891-5849(00)00161-1

Peroxiredoxin-controlled G-CSF signalling at the endoplasmic reticulum-early endosome interface
journal, November 2011

  • Palande, K.; Roovers, O.; Gits, J.
  • Journal of Cell Science, Vol. 124, Issue 21
  • DOI: 10.1242/jcs.089656

How pH Modulates the Dimer-Decamer Interconversion of 2-Cys Peroxiredoxins from the Prx1 Subfamily
journal, February 2015

  • Morais, Mariana A. B.; Giuseppe, Priscila O.; Souza, Tatiana A. C. B.
  • Journal of Biological Chemistry, Vol. 290, Issue 13
  • DOI: 10.1074/jbc.M114.619205

System-Wide Temporal Characterization of the Proteome and Phosphoproteome of Human Embryonic Stem Cell Differentiation
journal, March 2011


Quantitative analysis of a phosphoproteome readily altered by the protein kinase CK2 inhibitor quinalizarin in HEK-293T cells
journal, June 2015

  • Franchin, Cinzia; Cesaro, Luca; Salvi, Mauro
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1854, Issue 6
  • DOI: 10.1016/j.bbapap.2014.09.017

Enhanced Expression of Peroxiredoxin I and VI Correlates with Development, Recurrence and Progression of Human Bladder Cancer
journal, April 2006


Dimerization of thiol-specific antioxidant and the essential role of cysteine 47.
journal, July 1994

  • Chae, H. Z.; Uhm, T. B.; Rhee, S. G.
  • Proceedings of the National Academy of Sciences, Vol. 91, Issue 15
  • DOI: 10.1073/pnas.91.15.7022

Thiol peroxidases ameliorate LRRK2 mutant-induced mitochondrial and dopaminergic neuronal degeneration in Drosophila
journal, January 2014

  • Angeles, D. C.; Ho, P.; Chua, L. L.
  • Human Molecular Genetics, Vol. 23, Issue 12
  • DOI: 10.1093/hmg/ddu026

Reactive oxygen species in cancer
journal, January 2010


Free radicals, metals and antioxidants in oxidative stress-induced cancer
journal, March 2006


Mitogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity
journal, April 2009

  • Wu, Yongzheng; Feinstein, Sheldon I.; Manevich, Yefim
  • Biochemical Journal, Vol. 419, Issue 3
  • DOI: 10.1042/BJ20082061

Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation
journal, June 2013


Superoxide radicals as precursors of mitochondrial hydrogen peroxide
journal, May 1974


Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues
journal, January 2012

  • Lundby, Alicia; Secher, Anna; Lage, Kasper
  • Nature Communications, Vol. 3, Issue 1
  • DOI: 10.1038/ncomms1871

Two Enzymes in One
journal, May 2004


Stimulation of Peroxidase Activity by Decamerization Related to Ionic Strength: AhpC Protein from Amphibacillus xylanus
journal, August 1999


Toward a Comprehensive Characterization of a Human Cancer Cell Phosphoproteome
journal, December 2012

  • Zhou, Houjiang; Di Palma, Serena; Preisinger, Christian
  • Journal of Proteome Research, Vol. 12, Issue 1
  • DOI: 10.1021/pr300630k

Requirement for Generation of H(2)O(2) for Platelet-Derived Growth Factor Signal Tran sduction
journal, October 1995


Structure-based Insights into the Catalytic Power and Conformational Dexterity of Peroxiredoxins
journal, August 2011

  • Hall, Andrea; Nelson, Kimberly; Poole, Leslie B.
  • Antioxidants & Redox Signaling, Vol. 15, Issue 3
  • DOI: 10.1089/ars.2010.3624

Inactivation of Peroxiredoxin I by Phosphorylation Allows Localized H2O2 Accumulation for Cell Signaling
journal, February 2010


A role for 2-Cys peroxiredoxins in facilitating cytosolic protein thiol oxidation
journal, December 2017

  • Stöcker, Sarah; Maurer, Michael; Ruppert, Thomas
  • Nature Chemical Biology, Vol. 14, Issue 2
  • DOI: 10.1038/nchembio.2536

Immunoaffinity profiling of tyrosine phosphorylation in cancer cells
journal, December 2004

  • Rush, John; Moritz, Albrecht; Lee, Kimberly A.
  • Nature Biotechnology, Vol. 23, Issue 1
  • DOI: 10.1038/nbt1046

Phosphoproteomic differences in major depressive disorder postmortem brains indicate effects on synaptic function
journal, February 2012

  • Martins-de-Souza, Daniel; Guest, Paul C.; Vanattou-Saifoudine, Natacha
  • European Archives of Psychiatry and Clinical Neuroscience, Vol. 262, Issue 8
  • DOI: 10.1007/s00406-012-0301-3

Peroxiredoxin 5: Structure, Mechanism, and Function of the Mammalian Atypical 2-Cys Peroxiredoxin
journal, August 2011

  • Knoops, Bernard; Goemaere, Julie; Van der Eecken, Valérie
  • Antioxidants & Redox Signaling, Vol. 15, Issue 3
  • DOI: 10.1089/ars.2010.3584

Hydrogen peroxide is the major oxidant product of xanthine oxidase
journal, February 2010


Oligomeric peroxiredoxin-I is an essential intermediate for p53 to activate MST1 kinase and apoptosis
journal, April 2011


Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion
journal, November 2016

  • Sacco, Francesca; Humphrey, Sean J.; Cox, Jürgen
  • Nature Communications, Vol. 7, Issue 1
  • DOI: 10.1038/ncomms13250

Dimers to Doughnuts:  Redox-Sensitive Oligomerization of 2-Cysteine Peroxiredoxins ,
journal, April 2002

  • Wood, Zachary A.; Poole, Leslie B.; Hantgan, Roy R.
  • Biochemistry, Vol. 41, Issue 17
  • DOI: 10.1021/bi012173m

Loss of the Oxidative Stress Sensor NPGPx Compromises GRP78 Chaperone Activity and Induces Systemic Disease
journal, December 2012


Nrf2: friend or foe for chemoprevention?
journal, September 2009


Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells
journal, February 2012


Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
journal, January 2010

  • Song, H.; Mak, K. K.; Topol, L.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 4
  • DOI: 10.1073/pnas.0911409107

p10, the N-terminal domain of p35, protects against CDK5/p25-induced neurotoxicity
journal, November 2012

  • Zhang, L.; Liu, W.; Szumlinski, K. K.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 49
  • DOI: 10.1073/pnas.1212914109

Sensitive and nonenzymatic measurement of hydrogen peroxide in biological systems
journal, September 2000


Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1)
journal, June 2011


Epidermal Growth Factor (EGF)-induced Generation of Hydrogen Peroxide: ROLE IN EGF RECEPTOR-MEDIATED TYROSINE PHOSPHORYLATION
journal, January 1997

  • Bae, Yun Soo; Kang, Sang Won; Seo, Min Seok
  • Journal of Biological Chemistry, Vol. 272, Issue 1
  • DOI: 10.1074/jbc.272.1.217

The Emerging Role of Cdk5 in Cancer
journal, October 2016


Hydroperoxide metabolism in mammalian organs.
journal, July 1979


Mst1 and Mst2 protein kinases restrain intestinal stem cell proliferation and colonic tumorigenesis by inhibition of Yes-associated protein (Yap) overabundance
journal, October 2011

  • Zhou, D.; Zhang, Y.; Wu, H.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 49
  • DOI: 10.1073/pnas.1110428108

Quantitative Phospho-Proteomic Profiling of Hepatocyte Growth Factor (HGF)-MET Signaling in Colorectal Cancer
journal, July 2011

  • Organ, Shawna L.; Tong, Jiefei; Taylor, Paul
  • Journal of Proteome Research, Vol. 10, Issue 7
  • DOI: 10.1021/pr200238t

Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling
journal, November 2014

  • Sobotta, Mirko C.; Liou, Willy; Stöcker, Sarah
  • Nature Chemical Biology, Vol. 11, Issue 1
  • DOI: 10.1038/nchembio.1695

Phosphoproteomics Identifies Driver Tyrosine Kinases in Sarcoma Cell Lines and Tumors
journal, March 2012


Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression
journal, July 2003

  • Neumann, Carola A.; Krause, Daniela S.; Carman, Christopher V.
  • Nature, Vol. 424, Issue 6948
  • DOI: 10.1038/nature01819

Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor–dependent signaling pathways in renal collecting duct cells
journal, February 2010

  • Rinschen, Markus M.; Yu, Ming-Jiun; Wang, Guanghui
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 8
  • DOI: 10.1073/pnas.0910646107

Hippo signaling is a potent in vivo growth and tumor suppressor pathway in the mammalian liver
journal, January 2010

  • Lu, L.; Li, Y.; Kim, S. M.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 4
  • DOI: 10.1073/pnas.0911427107

Overexpression of peroxiredoxins I, II, III, V, and VI in malignant mesothelioma
journal, January 2002

  • Kinnula, V. L.; Lehtonen, S.; Sormunen, R.
  • The Journal of Pathology, Vol. 196, Issue 3
  • DOI: 10.1002/path.1042

A Phosphoproteomic Comparison of B-RAF V600E and MKK1/2 Inhibitors in Melanoma Cells
journal, April 2015

  • Stuart, Scott A.; Houel, Stephane; Lee, Thomas
  • Molecular & Cellular Proteomics, Vol. 14, Issue 6
  • DOI: 10.1074/mcp.M114.047233

Bioactive lipoxygenase metabolites stimulation of NADPH oxidases and reactive oxygen species
journal, March 2011


Regulation of PRDX1 peroxidase activity by Pin1
journal, March 2013

  • Chu, Kai Ling; Lew, Qiao Jing; Rajasegaran, Vikneswari
  • Cell Cycle, Vol. 12, Issue 6
  • DOI: 10.4161/cc.23916

Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry
journal, June 2015


Mammalian Peroxiredoxin Isoforms Can Reduce Hydrogen Peroxide Generated in Response to Growth Factors and Tumor Necrosis Factor-α
journal, March 1998

  • Kang, Sang Won; Chae, Ho Zoon; Seo, Min Seok
  • Journal of Biological Chemistry, Vol. 273, Issue 11
  • DOI: 10.1074/jbc.273.11.6297

Topology of Superoxide Production from Different Sites in the Mitochondrial Electron Transport Chain
journal, September 2002

  • St-Pierre, Julie; Buckingham, Julie A.; Roebuck, Stephen J.
  • Journal of Biological Chemistry, Vol. 277, Issue 47
  • DOI: 10.1074/jbc.M207217200

Reduction of Cysteine Sulfinic Acid in Eukaryotic, Typical 2-Cys Peroxiredoxins by Sulfiredoxin
journal, July 2011

  • Lowther, W. Todd; Haynes, Alexina C.
  • Antioxidants & Redox Signaling, Vol. 15, Issue 1
  • DOI: 10.1089/ars.2010.3564

Quantitative Phosphoproteomics Reveals Widespread Full Phosphorylation Site Occupancy During Mitosis
journal, January 2010


Deregulated Cdk5 promotes oxidative stress and mitochondrial dysfunction
journal, October 2008


Seleno-independent glutathione peroxidases: More than simple antioxidant scavengers
journal, April 2007


LRRK2 G2019S mutations are associated with an increased cancer risk in Parkinson disease : Cancer in
journal, September 2010

  • Saunders-Pullman, Rachel; Barrett, Matthew J.; Stanley, Kaili M.
  • Movement Disorders, Vol. 25, Issue 15
  • DOI: 10.1002/mds.23314

Structure and Mechanism of the Alkyl Hydroperoxidase AhpC, a Key Element of the Mycobacterium tuberculosis Defense System against Oxidative Stress
journal, May 2005

  • Guimarães, Beatriz G.; Souchon, Hélène; Honoré, Nadine
  • Journal of Biological Chemistry, Vol. 280, Issue 27
  • DOI: 10.1074/jbc.M503076200

Peroxiredoxin, a Novel Family of Peroxidases
journal, July 2001

  • Rhee, Sue Goo; Kang, Sang Won; Chang, Tong-Shin
  • IUBMB Life (International Union of Biochemistry and Molecular Biology: Life), Vol. 52, Issue 1
  • DOI: 10.1080/15216540252774748

Mice Deficient in Cellular Glutathione Peroxidase Develop Normally and Show No Increased Sensitivity to Hyperoxia
journal, June 1997

  • Ho, Ye-Shih; Magnenat, Jean-Luc; Bronson, Roderick T.
  • Journal of Biological Chemistry, Vol. 272, Issue 26
  • DOI: 10.1074/jbc.272.26.16644

mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes
journal, September 2016


Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death
journal, September 2011

  • Angeles, Dario C.; Gan, Bong-Hwa; Onstead, Luisa
  • Human Mutation, Vol. 32, Issue 12
  • DOI: 10.1002/humu.21582

A quantitative atlas of mitotic phosphorylation
journal, July 2008

  • Dephoure, N.; Zhou, C.; Villen, J.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 31
  • DOI: 10.1073/pnas.0805139105

The Refined Structure of the Selenoenzyme Glutathione Peroxidase at 0.2-nm Resolution
journal, June 1983


A Secreted Tyrosine Kinase Acts in the Extracellular Environment
journal, August 2014


Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics
journal, March 2015

  • Tsai, Chia-Feng; Wang, Yi-Ting; Yen, Hsin-Yung
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms7622

Recycling of peroxiredoxin IV provides a novel pathway for disulphide formation in the endoplasmic reticulum
journal, November 2010

  • Tavender, Timothy J.; Springate, Jennifer J.; Bulleid, Neil J.
  • The EMBO Journal, Vol. 29, Issue 24
  • DOI: 10.1038/emboj.2010.273

Diversity of properties among catalases
journal, May 2002


Cell-Specific Information Processing in Segregating Populations of Eph Receptor Ephrin-Expressing Cells
journal, December 2009


Hyperoxidation of Peroxiredoxins 2 and 3: RATE CONSTANTS FOR THE REACTIONS OF THE SULFENIC ACID OF THE PEROXIDATIC CYSTEINE
journal, March 2013

  • Peskin, Alexander V.; Dickerhof, Nina; Poynton, Rebecca A.
  • Journal of Biological Chemistry, Vol. 288, Issue 20
  • DOI: 10.1074/jbc.M113.460881

Quantitative Phosphoproteomics Identifies Substrates and Functional Modules of Aurora and Polo-Like Kinase Activities in Mitotic Cells
journal, June 2011


Peroxisomes Are Oxidative Organelles
journal, August 2010

  • Antonenkov, Vasily D.; Grunau, Silke; Ohlmeier, Steffen
  • Antioxidants & Redox Signaling, Vol. 13, Issue 4
  • DOI: 10.1089/ars.2009.2996

Essential Role of Cytoplasmic cdk5 and Prx2 in Multiple Ischemic Injury Models, In Vivo
journal, October 2009


Peroxiredoxins and Redox Signaling in Plants
journal, March 2018

  • Liebthal, Michael; Maynard, Daniel; Dietz, Karl-Josef
  • Antioxidants & Redox Signaling, Vol. 28, Issue 7
  • DOI: 10.1089/ars.2017.7164

Lysosomal ROS formation
journal, August 2005


Exploring cancer in LRRK2 mutation carriers and idiopathic Parkinson's disease
journal, December 2017

  • Warø, Bjørg Johanne; Aasly, Jan O.
  • Brain and Behavior, Vol. 8, Issue 1
  • DOI: 10.1002/brb3.858

Proteogenomics connects somatic mutations to signalling in breast cancer
journal, May 2016

  • Mertins, Philipp; Mani, D. R.; Ruggles, Kelly V.
  • Nature, Vol. 534, Issue 7605
  • DOI: 10.1038/nature18003

In-depth Analyses of Kinase-dependent Tyrosine Phosphoproteomes Based on Metal Ion-functionalized Soluble Nanopolymers
journal, June 2010

  • Iliuk, Anton B.; Martin, Victoria A.; Alicie, Bethany M.
  • Molecular & Cellular Proteomics, Vol. 9, Issue 10
  • DOI: 10.1074/mcp.M110.000091

Production of superoxide radicals and hydrogen peroxide by NADH-ubiquinone reductase and ubiquinol-cytochrome c reductase from beef-heart mitochondria
journal, April 1977

  • Cadenas, Enrique; Boveris, Alberto; Ragan, C. Ian
  • Archives of Biochemistry and Biophysics, Vol. 180, Issue 2
  • DOI: 10.1016/0003-9861(77)90035-2

Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice
journal, February 2003


An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome
journal, January 2014


Peroxiredoxins, a novel protein family in lung cancer: Peroxiredoxins in Lung Cancer
journal, May 2004

  • Lehtonen, Siri T.; Svensk, Anne-Mari; Soini, Ylermi
  • International Journal of Cancer, Vol. 111, Issue 4
  • DOI: 10.1002/ijc.20294

Polarographic assay and intracellular distribution of superoxide dismutase in rat liver.
journal, June 1975


New roles for old pathways? A circuitous relationship between reactive oxygen species and cyclo-oxygenase in hypertension
journal, September 2013

  • Hernanz, Raquel; Briones, Ana M.; Salaices, Mercedes
  • Clinical Science, Vol. 126, Issue 2
  • DOI: 10.1042/CS20120651

AKT1 and AKT2 Induce Distinct Phosphorylation Patterns in HL-1 Cardiac Myocytes
journal, September 2014

  • Reinartz, Michael; Raupach, Annika; Kaisers, Wolfgang
  • Journal of Proteome Research, Vol. 13, Issue 10
  • DOI: 10.1021/pr500131g

Characterization of Superoxide-producing Sites in Isolated Brain Mitochondria
journal, November 2003

  • Kudin, Alexei P.; Bimpong-Buta, Nana Yaw-B.; Vielhaber, Stefan
  • Journal of Biological Chemistry, Vol. 279, Issue 6
  • DOI: 10.1074/jbc.M310341200

Ultradeep Human Phosphoproteome Reveals a Distinct Regulatory Nature of Tyr and Ser/Thr-Based Signaling
journal, September 2014


Overexpression of Prdx6 and resistance to peroxide-induced death in Hepa1-6 cells: Prdx suppression increases apoptosis
journal, December 2009


Peroxiredoxin 1 and its role in cell signaling
journal, December 2009

  • Neumann, Carola A.; Cao, Juxiang; Manevich, Yefim
  • Cell Cycle, Vol. 8, Issue 24
  • DOI: 10.4161/cc.8.24.10242

Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells
journal, April 2014

  • Yi, T.; Zhai, B.; Yu, Y.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 21
  • DOI: 10.1073/pnas.1404943111

Reactive oxygen species and cancer paradox: To promote or to suppress?
journal, March 2017


Identification of Targets of c-Src Tyrosine Kinase by Chemical Complementation and Phosphoproteomics
journal, April 2012

  • Ferrando, Isabel Martinez; Chaerkady, Raghothama; Zhong, Jun
  • Molecular & Cellular Proteomics, Vol. 11, Issue 8
  • DOI: 10.1074/mcp.M111.015750

A probability-based approach for high-throughput protein phosphorylation analysis and site localization
journal, September 2006

  • Beausoleil, Sean A.; Villén, Judit; Gerber, Scott A.
  • Nature Biotechnology, Vol. 24, Issue 10
  • DOI: 10.1038/nbt1240

Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2
journal, June 2013


Reversible Inactivation of Protein-tyrosine Phosphatase 1B in A431 Cells Stimulated with Epidermal Growth Factor
journal, June 1998

  • Lee, Seung-Rock; Kwon, Ki-Sun; Kim, Seung-Ryul
  • Journal of Biological Chemistry, Vol. 273, Issue 25
  • DOI: 10.1074/jbc.273.25.15366

Brain Phosphoproteome Obtained by a FASP-Based Method Reveals Plasma Membrane Protein Topology
journal, June 2010

  • Wiśniewski, Jacek R.; Nagaraj, Nagarjuna; Zougman, Alexandre
  • Journal of Proteome Research, Vol. 9, Issue 6
  • DOI: 10.1021/pr1002214

NADPH Oxidase Biology and the Regulation of Tyrosine Kinase Receptor Signaling and Cancer Drug Cytotoxicity
journal, February 2013

  • Paletta-Silva, Rafael; Rocco-Machado, Nathália; Meyer-Fernandes, José
  • International Journal of Molecular Sciences, Vol. 14, Issue 2
  • DOI: 10.3390/ijms14023683

Peroxiredoxin 1 functions as a signal peroxidase to receive, transduce, and transmit peroxide signals in mammalian cells
journal, October 2012


Hydrogen Peroxide: A Signaling Messenger
journal, March 2006


The mechanism of superoxide production by NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria
journal, May 2006

  • Kussmaul, L.; Hirst, J.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 20
  • DOI: 10.1073/pnas.0510977103

Mice Lacking Catalase Develop Normally but Show Differential Sensitivity to Oxidant Tissue Injury
journal, June 2004

  • Ho, Ye-Shih; Xiong, Ye; Ma, Wanchao
  • Journal of Biological Chemistry, Vol. 279, Issue 31
  • DOI: 10.1074/jbc.M404800200

Peroxiredoxin-null Yeast Cells Are Hypersensitive to Oxidative Stress and Are Genomically Unstable
journal, March 2004

  • Wong, Chi-Ming; Siu, Kam-Leung; Jin, Dong-Yan
  • Journal of Biological Chemistry, Vol. 279, Issue 22
  • DOI: 10.1074/jbc.M402095200

Phosphoproteomic Analysis of Human Embryonic Stem Cells
journal, August 2009


Cooperation of Yeast Peroxiredoxins Tsa1p and Tsa2p in the Cellular Defense against Oxidative and Nitrosative Stress
journal, December 2001

  • Wong, Chi-Ming; Zhou, Yuan; Ng, Raymond W. M.
  • Journal of Biological Chemistry, Vol. 277, Issue 7
  • DOI: 10.1074/jbc.M106846200

Phospholipase A 2 Activity of Peroxiredoxin 6 Promotes Invasion and Metastasis of Lung Cancer Cells
journal, March 2010


The Plk1-dependent Phosphoproteome of the Early Mitotic Spindle
journal, September 2010

  • Santamaria, Anna; Wang, Bin; Elowe, Sabine
  • Molecular & Cellular Proteomics, Vol. 10, Issue 1
  • DOI: 10.1074/mcp.M110.004457