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Title: Resistance and Resilience of Sulfidogenic Communities in the Face of the Specific Inhibitor Perchlorate

Abstract

Hydrogen sulfide is a toxic and corrosive gas, produced by the activity of sulfate-reducing microorganisms (SRM). Owing to the environmental, economic and human-health consequences of sulfide, there is interest in developing specific inhibitors of SRM. Recent studies have identified perchlorate as a promising emerging inhibitor. The aim of this work is to quantitatively dissect the inhibitory dynamics of perchlorate. Sulfidogenic mixed continuous-flow systems were treated with perchlorate. SRM number, sulfide production and community structure were monitored pre-, during and post-treatment. The data generated was compared to a simple mathematical model, where SRM growth slows as a result of inhibition. The experimental data supports the interpretation that perchlorate largely acts to suppress SRM growth rates, rendering planktonic SRM increasingly susceptible to wash-out. Surface-attachment was identified as an important parameter preventing SRM wash-out and thus governing inhibitory dynamics. Our study confirmed the lesser depletion of surface-attached SRM as compared to planktonic SRM during perchlorate treatment. Indirect effects of perchlorate (bio-competitive exclusion of SRM by dissimilatory perchlorate-reducing bacteria, DPRB) were also assayed by amending reactors with DPRB. Indeed, low concentrations of perchlorate coupled with DRPB amendment can drive sulfide concentrations to zero. Further, inhibition in a complex community was compared to that inmore » a pure culture, highlighting similarities and differences between the two scenarios. Finally, we quantified susceptibility to perchlorate across SRM in various culture conditions, showing that prediction of complex behavior in continuous systems from batch results is possible. This study thus provides an overview of the sensitivity of sulfidogenic communities to perchlorate, as well as mechanisms underlying these patterns.« less

Authors:
 [1];  [2];  [2];  [3];  [1];  [4];  [4]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; Energy Biosciences Inst., Berkeley, CA (United States)
  2. Univ. of California, Berkeley, CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  4. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; Energy Biosciences Inst., Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1560581
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 10; Journal Issue: APR; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
perchlorate; specific inhibition; sulfate reduction; chemostat; microbial communities; surface attachment; growth rate

Citation Formats

Stoeva, Magdalena K., Nalula, Gilbert, Garcia, Nicholas, Cheng, Yiwei, Engelbrektson, Anna L., Carlson, Hans K., and Coates, John D. Resistance and Resilience of Sulfidogenic Communities in the Face of the Specific Inhibitor Perchlorate. United States: N. p., 2019. Web. doi:10.3389/fmicb.2019.00654.
Stoeva, Magdalena K., Nalula, Gilbert, Garcia, Nicholas, Cheng, Yiwei, Engelbrektson, Anna L., Carlson, Hans K., & Coates, John D. Resistance and Resilience of Sulfidogenic Communities in the Face of the Specific Inhibitor Perchlorate. United States. doi:10.3389/fmicb.2019.00654.
Stoeva, Magdalena K., Nalula, Gilbert, Garcia, Nicholas, Cheng, Yiwei, Engelbrektson, Anna L., Carlson, Hans K., and Coates, John D. Tue . "Resistance and Resilience of Sulfidogenic Communities in the Face of the Specific Inhibitor Perchlorate". United States. doi:10.3389/fmicb.2019.00654. https://www.osti.gov/servlets/purl/1560581.
@article{osti_1560581,
title = {Resistance and Resilience of Sulfidogenic Communities in the Face of the Specific Inhibitor Perchlorate},
author = {Stoeva, Magdalena K. and Nalula, Gilbert and Garcia, Nicholas and Cheng, Yiwei and Engelbrektson, Anna L. and Carlson, Hans K. and Coates, John D.},
abstractNote = {Hydrogen sulfide is a toxic and corrosive gas, produced by the activity of sulfate-reducing microorganisms (SRM). Owing to the environmental, economic and human-health consequences of sulfide, there is interest in developing specific inhibitors of SRM. Recent studies have identified perchlorate as a promising emerging inhibitor. The aim of this work is to quantitatively dissect the inhibitory dynamics of perchlorate. Sulfidogenic mixed continuous-flow systems were treated with perchlorate. SRM number, sulfide production and community structure were monitored pre-, during and post-treatment. The data generated was compared to a simple mathematical model, where SRM growth slows as a result of inhibition. The experimental data supports the interpretation that perchlorate largely acts to suppress SRM growth rates, rendering planktonic SRM increasingly susceptible to wash-out. Surface-attachment was identified as an important parameter preventing SRM wash-out and thus governing inhibitory dynamics. Our study confirmed the lesser depletion of surface-attached SRM as compared to planktonic SRM during perchlorate treatment. Indirect effects of perchlorate (bio-competitive exclusion of SRM by dissimilatory perchlorate-reducing bacteria, DPRB) were also assayed by amending reactors with DPRB. Indeed, low concentrations of perchlorate coupled with DRPB amendment can drive sulfide concentrations to zero. Further, inhibition in a complex community was compared to that in a pure culture, highlighting similarities and differences between the two scenarios. Finally, we quantified susceptibility to perchlorate across SRM in various culture conditions, showing that prediction of complex behavior in continuous systems from batch results is possible. This study thus provides an overview of the sensitivity of sulfidogenic communities to perchlorate, as well as mechanisms underlying these patterns.},
doi = {10.3389/fmicb.2019.00654},
journal = {Frontiers in Microbiology},
number = APR,
volume = 10,
place = {United States},
year = {2019},
month = {4}
}

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Works referenced in this record:

Reduction of (per)chlorate by a novel organism isolated from paper mill waste
journal, August 1999


Spectrophotometric Determination Of Hydrogen Sulfide In Natural Waters
journal, May 1969


Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
journal, October 2009

  • Schloss, P. D.; Westcott, S. L.; Ryabin, T.
  • Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541
  • DOI: 10.1128/AEM.01541-09