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Title: Lessons from bacteriophages part 1: Deriving utility from protein structure, function, and evolution

Abstract

Historically, some of the most fundamental discoveries of modern molecular biology were revealed by examination of phage-infected cells. Some examples include the use of bacteriophage T2 to show that DNA, not protein, was the genetic material by Hershey and Chase in 1952. Crick and colleagues showed that codons are degenerate and encode for single amino acids by making use of T4 bacteriophage. Brenner and colleagues discovered that mRNA acts as the messenger in relaying the information from DNA to ribosomes with the aid of bacteriophage T2. Szybalski and colleagues demonstrated the semiconservative mechanism of DNA replication using λ phage. Restriction modification, a bacterial innate immune system that evolved as protection against invading mobile genetic elements such as plasmids and phages, was discovered in bacteriophage-infected cells. Restriction mapping was crucial for the completion of the human genome project. The mechanism of action of heat shock genes—which encode for molecular chaperones such as dnaK, dnaJ, and grpE, as well as the groE gene locus, which encodes for the molecular chaperones GroES and GroEL—were discovered in λ phage–infected Escherichia coli. The first capsid assembly inhibitor, the fluorescent dye 4,4’–bis(1-anilinonaphthalene-8-sulfonic acid) (bisANS), was discovered in experiments investigating assembly of phage P22. These are justmore » some of the examples of contributions made by bacteriophage research in the past few decades. Here, we discuss additional discoveries derived from studies of phages.« less

Authors:
 [1]; ORCiD logo [1];  [1]
  1. Univ. of Connecticut, Storrs, CT (United States). Dept. of Molecular and Cell Biology
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1560552
Grant/Contract Number:  
AC02-05CH11231; R01 GM076661
Resource Type:
Accepted Manuscript
Journal Name:
PLoS Pathogens
Additional Journal Information:
Journal Volume: 14; Journal Issue: 5; Journal ID: ISSN 1553-7374
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Asija, Kunica, Teschke, Carolyn M., and Dutch, Rebecca Ellis. Lessons from bacteriophages part 1: Deriving utility from protein structure, function, and evolution. United States: N. p., 2018. Web. doi:10.1371/journal.ppat.1006971.
Asija, Kunica, Teschke, Carolyn M., & Dutch, Rebecca Ellis. Lessons from bacteriophages part 1: Deriving utility from protein structure, function, and evolution. United States. doi:10.1371/journal.ppat.1006971.
Asija, Kunica, Teschke, Carolyn M., and Dutch, Rebecca Ellis. Thu . "Lessons from bacteriophages part 1: Deriving utility from protein structure, function, and evolution". United States. doi:10.1371/journal.ppat.1006971. https://www.osti.gov/servlets/purl/1560552.
@article{osti_1560552,
title = {Lessons from bacteriophages part 1: Deriving utility from protein structure, function, and evolution},
author = {Asija, Kunica and Teschke, Carolyn M. and Dutch, Rebecca Ellis},
abstractNote = {Historically, some of the most fundamental discoveries of modern molecular biology were revealed by examination of phage-infected cells. Some examples include the use of bacteriophage T2 to show that DNA, not protein, was the genetic material by Hershey and Chase in 1952. Crick and colleagues showed that codons are degenerate and encode for single amino acids by making use of T4 bacteriophage. Brenner and colleagues discovered that mRNA acts as the messenger in relaying the information from DNA to ribosomes with the aid of bacteriophage T2. Szybalski and colleagues demonstrated the semiconservative mechanism of DNA replication using λ phage. Restriction modification, a bacterial innate immune system that evolved as protection against invading mobile genetic elements such as plasmids and phages, was discovered in bacteriophage-infected cells. Restriction mapping was crucial for the completion of the human genome project. The mechanism of action of heat shock genes—which encode for molecular chaperones such as dnaK, dnaJ, and grpE, as well as the groE gene locus, which encodes for the molecular chaperones GroES and GroEL—were discovered in λ phage–infected Escherichia coli. The first capsid assembly inhibitor, the fluorescent dye 4,4’–bis(1-anilinonaphthalene-8-sulfonic acid) (bisANS), was discovered in experiments investigating assembly of phage P22. These are just some of the examples of contributions made by bacteriophage research in the past few decades. Here, we discuss additional discoveries derived from studies of phages.},
doi = {10.1371/journal.ppat.1006971},
journal = {PLoS Pathogens},
number = 5,
volume = 14,
place = {United States},
year = {2018},
month = {5}
}

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Figures / Tables:

Fig 1 Fig 1: (A) Capsid structure of bacteriophage P22. The left side of panel A shows the mature P22 capsid. The pentons are highlighted in blue, and hexons are shown in gray. The asymmetric unit (seven subunits) of the capsid is represented in ribbon format in the center of the capsid.more » The asymmetric unit is magnified and shown on the right of the panel. (B) Ribbon diagrams of coat monomers of P22 (left) and HK97 (right). The Adomains, P-domains, E-loops, and N-arms have been highlighted in cyan, green, yellow, and red, respectively. The I-domain of P22 coat protein has been highlighted in coral. The arrows indicate the D-loop (purple) of the monomer of P22 (left) and the G-loop (purple) on the monomer of HK97 (right).« less

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