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Title: Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32

Abstract

The bioreduction of Fe(III) oxides by dissimilatory iron reducing bacteria (DIRB) may result in the production of a suite of Fe(II)-bearing secondary minerals, including magnetite, siderite, vivianite, green rusts, and chukanovite; the formation of specific phases controlled by the interaction of various physiological and geochemical factors. In an effort to better understand the effects of individual electron donors on the formation of specific Fe(II)-bearing secondary minerals, we examined the effects of a series of potential electron donors on the bioreduction of lepidocrocite ($γ$-FeOOH) by Shewanella putrefaciens CN32. Biomineralization products were identified by X-ray diffraction, Mössbauer spectroscopy, and scanning electron microscopy. Acetate, citrate, ethanol, glucose, glutamate, glycerol, malate, and succinate were not effectively utilized for the bioreduction of lepidocrocite by S. putrefaciens CN32; however, substantial Fe(II) production was observed when formate, lactate, H 2, pyruvate, serine, or N acetylglucosamine (NAG) was provided as an electron donor. Carbonate or sulfate green rust was the dominant Fe(II)-bearing secondary mineral when formate, H 2, lactate, or NAG was provided, however, siderite formed with pyruvate or serine. Geochemical modeling indicated that pH and carbonate concentration are the key factors determining the prevalence of carbonate green rust verses siderite.

Authors:
 [1]; ORCiD logo [2]; ORCiD logo [3];  [4]
  1. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Div.
  2. Univ. of Iowa, Iowa City, IA (United States). Dept. of Civil and Environmental Engineering; Pennsylvania State Univ., University Park, PA (United States). Dept. of Civil and Environmental Engineering
  3. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Div.; California Dept. of Water Resources, Sacramento, CA (United States)
  4. Univ. of Iowa, Iowa City, IA (United States). Dept. of Civil and Environmental Engineering
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science - Office of Biological and Environmental Research - Subsurface Biogeochemical Research
OSTI Identifier:
1560537
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Minerals
Additional Journal Information:
Journal Volume: 9; Journal Issue: 7; Journal ID: ISSN 2075-163X
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
58 GEOSCIENCES; dissimilatory iron reduction; green rust; iron oxide; siderite

Citation Formats

O’Loughlin, Edward J., Gorski, Christopher A., Flynn, Theodore M., and Scherer, Michelle M. Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32. United States: N. p., 2019. Web. doi:10.3390/min9070434.
O’Loughlin, Edward J., Gorski, Christopher A., Flynn, Theodore M., & Scherer, Michelle M. Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32. United States. doi:10.3390/min9070434.
O’Loughlin, Edward J., Gorski, Christopher A., Flynn, Theodore M., and Scherer, Michelle M. Sun . "Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32". United States. doi:10.3390/min9070434. https://www.osti.gov/servlets/purl/1560537.
@article{osti_1560537,
title = {Electron Donor Utilization and Secondary Mineral Formation during the Bioreduction of Lepidocrocite by Shewanella putrefaciens CN32},
author = {O’Loughlin, Edward J. and Gorski, Christopher A. and Flynn, Theodore M. and Scherer, Michelle M.},
abstractNote = {The bioreduction of Fe(III) oxides by dissimilatory iron reducing bacteria (DIRB) may result in the production of a suite of Fe(II)-bearing secondary minerals, including magnetite, siderite, vivianite, green rusts, and chukanovite; the formation of specific phases controlled by the interaction of various physiological and geochemical factors. In an effort to better understand the effects of individual electron donors on the formation of specific Fe(II)-bearing secondary minerals, we examined the effects of a series of potential electron donors on the bioreduction of lepidocrocite ($γ$-FeOOH) by Shewanella putrefaciens CN32. Biomineralization products were identified by X-ray diffraction, Mössbauer spectroscopy, and scanning electron microscopy. Acetate, citrate, ethanol, glucose, glutamate, glycerol, malate, and succinate were not effectively utilized for the bioreduction of lepidocrocite by S. putrefaciens CN32; however, substantial Fe(II) production was observed when formate, lactate, H2, pyruvate, serine, or N acetylglucosamine (NAG) was provided as an electron donor. Carbonate or sulfate green rust was the dominant Fe(II)-bearing secondary mineral when formate, H2, lactate, or NAG was provided, however, siderite formed with pyruvate or serine. Geochemical modeling indicated that pH and carbonate concentration are the key factors determining the prevalence of carbonate green rust verses siderite.},
doi = {10.3390/min9070434},
journal = {Minerals},
number = 7,
volume = 9,
place = {United States},
year = {2019},
month = {7}
}

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Works referenced in this record:

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