Absence of estrogen receptor beta leads to abnormal adipogenesis during early tendon healing by an up‐regulation of PPARγ signalling
[1];
[1];
- Department of Orthopedic Surgery Southwest Hospital, Army Medical University Chongqing China
- Department of Developmental Neuropsychology, School of Psychology Third Military Medical University, Army Medical University Chongqing China
- Center for Nuclear Receptors and Cell Signaling University of Houston Houston TX, Department of Biosciences and Nutrition, Center for Innovative Medicine Karolinska Institute Novum Sweden
Abstract Achilles tendon injury is one of the challenges of sports medicine, the aetiology of which remains unknown. For a long time, estrogen receptor β (ERβ) has been known as a regulating factor of the metabolism in many connective tissues, such as bone, muscle and cartilage, but little is known about its role in tendon. Recent studies have implicated ERβ as involved in the process of tendon healing. Tendon‐derived stem cells (TDSCs) are getting more and more attention in tendon physiological and pathological process. In this study, we investigated how ERβ played a role in Achilles tendon healing. Achilles tendon injury model was established to analyse how ERβ affected on healing process in vivo. Cell proliferation assay, Western blots, qRT‐PCR and immunocytochemistry were performed to investigate the effect of ERβ on TDSCs. Here, we showed that ERβ deletion in mice resulted in inferior gross appearance, histological scores and, most importantly, increased accumulation of adipocytes during the early tendon healing which involved activation of peroxisome proliferator‐activated receptor γ (PPARγ) signalling. Furthermore, in vitro results of ours confirmed that the abnormity might be the result of abnormal TDSC adipogenic differentiation which could be partially reversed by the treatment of ERβ agonist LY3201. These data revealed a role of ERβ in Achilles tendon healing for the first time, thereby providing a new target for clinical treatment of Achilles tendon injury.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1560237
- Alternate ID(s):
- OSTI ID: 1560238
- Journal Information:
- Journal of Cellular and Molecular Medicine, Journal Name: Journal of Cellular and Molecular Medicine Journal Issue: 11 Vol. 23; ISSN 1582-1838
- Publisher:
- Wiley-BlackwellCopyright Statement
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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