SupraCells: Living Mammalian Cells Protected within Functional Modular Nanoparticle‐Based Exoskeletons
[3];
[4];
[4];
[5];
[2];
[2]
- School of Biology and Biological Engineering South China University of Technology 382 East Outer Loop Road, University Park Guangzhou 510006 P. R. China, Center for Micro‐Engineered Materials Department of Chemical and Biological Engineering The University of New Mexico Albuquerque NM 87131 USA
- Center for Micro‐Engineered Materials Department of Chemical and Biological Engineering The University of New Mexico Albuquerque NM 87131 USA
- Max Planck Institute of Colloids and Interfaces Am Mühlenberg 1 14476 Potsdam Germany
- ARC Centre of Excellence in Convergent Bio‐Nano Science and Technology Department of Chemical and Biomolecular Engineering The University of Melbourne Parkville Victoria 3010 Australia
- Department of Chemistry and Center for NanoScience (CeNS) University of Munich (LMU) Butenandtstraße 11 81377 Munich Germany
- School of Energy and Environment City University of Hong Kong Tat Chee Avenue Kowloon Hong Kong SAR P. R. China
Abstract Creating a synthetic exoskeleton from abiotic materials to protect delicate mammalian cells and impart them with new functionalities could revolutionize fields like cell‐based sensing and create diverse new cellular phenotypes. Herein, the concept of “SupraCells,” which are living mammalian cells encapsulated and protected within functional modular nanoparticle‐based exoskeletons, is introduced. Exoskeletons are generated within seconds through immediate interparticle and cell/particle complexation that abolishes the macropinocytotic and endocytotic nanoparticle internalization pathways that occur without complexation. SupraCell formation is shown to be generalizable to wide classes of nanoparticles and various types of cells. It induces a spore‐like state, wherein cells do not replicate or spread on surfaces but are endowed with extremophile properties, for example, resistance to osmotic stress, reactive oxygen species, pH, and UV exposure, along with abiotic properties like magnetism, conductivity, and multifluorescence. Upon decomplexation cells return to their normal replicative states. SupraCells represent a new class of living hybrid materials with a broad range of functionalities.
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- NA0003525
- OSTI ID:
- 1560213
- Journal Information:
- Advanced Materials, Journal Name: Advanced Materials Journal Issue: 25 Vol. 31; ISSN 0935-9648
- Publisher:
- Wiley Blackwell (John Wiley & Sons)Copyright Statement
- Country of Publication:
- Germany
- Language:
- English
Similar Records
Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus
FRET sensor-based quantification of intracellular trehalose in mammalian cells