Title: Rhizobacteria Mediate the Phytotoxicity of a Range of Biorefinery‐Relevant Compounds

Journal Article · · Environmental Toxicology and Chemistry
DOI: https://doi.org/10.1002/etc.4501 · OSTI ID:1560185
 [1];  [1];  [2];  [3];  [3];  [3];  [1];  [3];  [4];  [3];  [5];  [6];  [6]
  1. Biological Systems and Engineering Division Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley California USA, Joint BioEnergy Institute, Emeryville California USA
  2. Biological Systems and Engineering Division Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley California USA, College of Science and Engineering San Francisco State University, San Francisco California USA
  3. Biological Systems and Engineering Division Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley California USA
  4. College of Science and Engineering San Francisco State University, San Francisco California USA
  5. Biological Systems and Engineering Division Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley California USA, Environmental Genomics and System Biology Division Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley California USA
  6. Biological Systems and Engineering Division Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley California USA, Joint BioEnergy Institute, Emeryville California USA, Environmental Genomics and System Biology Division Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley California USA

Abstract Advances in engineering biology have expanded the list of renewable compounds that can be produced at scale via biological routes from plant biomass. In most cases, these chemical products have not been evaluated for effects on biological systems, defined in the present study as bioactivity, that may be relevant to their manufacture. For sustainable chemical and fuel production, the industry needs to transition from fossil to renewable carbon sources, resulting in unprecedented expansion in the production and environmental distribution of chemicals used in biomanufacturing. Further, although some chemicals have been assessed for mammalian toxicity, environmental and agricultural hazards are largely unknown. We assessed 6 compounds that are representative of the emerging biofuel and bioproduct manufacturing process for their effect on model plants ( Arabidopsis thaliana , Sorghum bicolor ) and show that several alter plant seedling physiology at submillimolar concentrations. However, these responses change in the presence of individual bacterial species from the A. thaliana root microbiome. We identified 2 individual microbes that change the effect of chemical treatment on root architecture and a pooled microbial community with different effects relative to its constituents individually. The present study indicates that screening industrial chemicals for bioactivity on model organisms in the presence of their microbiomes is important for biologically and ecologically relevant risk analyses. Environ Toxicol Chem 2019;38:1911–1922. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1560185
Journal Information:
Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 9 Vol. 38; ISSN 0730-7268
Publisher:
Wiley Blackwell (John Wiley & Sons)Copyright Statement
Country of Publication:
United States
Language:
English

References (54)

Improving methyl ketone production in Escherichia coli by heterologous expression of NADH-dependent FabG journal March 2018
How omics technologies can enhance chemical safety regulation: perspectives from academia, government, and industry journal April 2018
From climate chambers to biodiversity chambers journal April 2018
Trends in pesticide use on soybean, corn and cotton since the introduction of major genetically modified crops in the United States: Pesticide use on US soybean, corn and cotton since the introduction of GM crops journal August 2015
Endophytic microbes Bacillus sp. LZR216-regulated root development is dependent on polar auxin transport in Arabidopsis seedlings journal February 2015
Auxins and Tropisms journal September 2001
Role of cytokinin in the regulation of root gravitropism journal September 2004
Mitsuaria sp. and Burkholderia sp. from Arabidopsis rhizosphere enhance drought tolerance in Arabidopsis thaliana and maize (Zea mays L.) journal August 2017
Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production journal February 2017
Characterization of white poplar and eucalyptus after ionic liquid pretreatment as a function of biomass loading using X-ray diffraction and small angle neutron scattering journal May 2017
d-Limonene modulates T lymphocyte activity and viability journal September 2012
Review of toxicological assessment of d-limonene, a food and cosmetics additive journal October 2018
Enhancing the antimicrobial activity of d-limonene nanoemulsion with the inclusion of ε-polylysine journal April 2017
Hydrogenated monoterpenes as diesel fuel additives journal November 2009
The therapeutic efficacy of α-pinene in an experimental mouse model of allergic rhinitis journal November 2014
Understanding and exploiting plant beneficial microbes journal August 2017
Plant growth-promoting bacteria confer resistance in tomato plants to salt stress journal June 2004
Ionic liquids as herbicides and plant growth regulators journal June 2013
Lateral root development in Arabidopsis: fifty shades of auxin journal August 2013
Gut microbiome interactions with drug metabolism, efficacy, and toxicity journal January 2017
Life-Cycle Greenhouse Gas and Water Intensity of Cellulosic Biofuel Production Using Cholinium Lysinate Ionic Liquid Pretreatment journal September 2017
High-Density Renewable Fuels Based on the Selective Dimerization of Pinenes journal January 2010
Engineering Terpene Biosynthesis in Streptomyces for Production of the Advanced Biofuel Precursor Bisabolene journal July 2014
Defining the core Arabidopsis thaliana root microbiome journal August 2012
Multi-level engineering facilitates the production of phenylpropanoid compounds in tomato journal October 2015
NIH Image to ImageJ: 25 years of image analysis journal June 2012
Genomic features of bacterial adaptation to plants journal December 2017
Toxicity of Pine Monoterpenes to Mountain Pine Beetle journal August 2017
Understanding pretreatment efficacy of four cholinium and imidazolium ionic liquids by chemistry and computation journal January 2014
Transformation of Arabidopsis thaliana with the codA gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress journal July 1997
Transcriptome analysis of Arabidopsis colonized by a plant-growth promoting rhizobacterium reveals a general effect on disease resistance journal October 2003
Lignin valorization through integrated biological funneling and chemical catalysis journal August 2014
Interactive Tree Of Life v2: online annotation and display of phylogenetic trees made easy journal April 2011
Exploiting the Substrate Promiscuity of Hydroxycinnamoyl-CoA:Shikimate Hydroxycinnamoyl Transferase to Reduce Lignin journal February 2016
Mitogen-Activated Protein Kinase 6 and Ethylene and Auxin Signaling Pathways Are Involved in Arabidopsis Root-System Architecture Alterations by Trichoderma atroviride journal June 2015
Osmoregulation in Azospirillum brasilense: glycine betaine transport enhances growth and nitrogen fixation under salt stress journal August 1990
A Novel Image-Analysis Toolbox Enabling Quantitative Analysis of Root System Architecture journal July 2011
Limonene hydroperoxide analogues differ in allergenic activity journal December 2008
Expression of a bacterial 3-dehydroshikimate dehydratase reduces lignin content and improves biomass saccharification efficiency journal January 2015
Acute Limonene Toxicity in Escherichia coli Is Caused by Limonene Hydroperoxide and Alleviated by a Point Mutation in Alkyl Hydroperoxidase AhpC journal May 2015
Plant synthetic biology could drive a revolution in biofuels and medicine journal September 2018
Trends in glyphosate herbicide use in the United States and globally journal February 2016
Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification journal September 2018
Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates journal February 2019
Design of synthetic bacterial communities for predictable plant phenotypes journal February 2018
Deciphering microbial interactions in synthetic human gut microbiome communities journal June 2018
Limonene, a Citrus Extract, for Control of Mealybugs and Scale Insects journal June 2005
2016 Billion-Ton Report: Advancing Domestic Resources for a Thriving Bioeconomy report July 2016
Expression of S-adenosylmethionine Hydrolase in Tissues Synthesizing Secondary Cell Walls Alters Specific Methylated Cell Wall Fractions and Improves Biomass Digestibility journal July 2016
Klebsiella pneumoniae SnebYK Mediates Resistance Against Heterodera glycines and Promotes Soybean Growth journal June 2018
Burkholderia phytofirmans PsJN induces long-term metabolic and transcriptional changes involved in Arabidopsis thaliana salt tolerance journal June 2015
Biological Activities of a-Pinene and β-Pinene Enantiomers journal May 2012
Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions journal January 2018
Understanding the impact of ionic liquid pretreatment on eucalyptus journal January 2010