Periodic Arrangement of Lipopolysaccharides Nanostructures Accelerates and Enhances the Maturation Processes of Dendritic Cells

doi:10.1021/acsanm.7b00254

Title: Periodic Arrangement of Lipopolysaccharides Nanostructures Accelerates and Enhances the Maturation Processes of Dendritic Cells

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Abstract

This work reports an important application of nanomaterials consisting of biological ligands, such as lipopolysaccharides (LPS) nanostructures. Upon immobilization onto surfaces as periodic arrays of nanostructures, these surface-bound LPS nanostructures significantly impacted the maturation process of bone marrow derived dendritic cells (BMDCs): accelerating the maturation process in comparison to other means of ligand presentations such as LPS solution and monolayers on surfaces. In some cases, LPS nanostructures led to a new maturation phenotype in vitro characterized by hyper-dendritized morphology. This level of maturation enhancement was observed for the first time in vitro. Possible mechanisms leading to the acceleration and enhancement of BMDC maturation were also discussed. This work demonstrates an important concept: surface-bound nanostructures of ligands provide a new and powerful cue for regulating cellular signaling processes. The fact that primary cells’ signaling processes, such as BMDC maturation, can be altered suggests a new means for programming dendritic cells for immune therapy.

Authors:

Liu, Yang ^[1]; Wang, Kang-Hsin ^[2]; Chen, Huan-Yuan ^[3]; Li, Jie-Ren ^[1]; Laurence, Ted A. ^[4]; Ly, Sonny ^[4]; Liu, Fu-Tong ^[5];

^[1]

Univ. of California, Davis, CA (United States). Dept. of Chemistry
Univ. of California, Davis, CA (United States). Dept. of Chemistry and Dept. of Dermatology, School of Medicine
Univ. of California, Davis, CA (United States). Dept. of Dermatology, School of Medicine; Academia Sinica, Taipei, (Taiwan). Inst. of Biomedical Sciences
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Univ. of California, Sacramento, CA (United States). Dept. of Dermatology, School of Medicine; Academia Sinica, Taipei, (Taiwan). Inst. of Biomedical Sciences

Publication Date:: 2018-01-29

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE National Nuclear Security Administration (NNSA)

OSTI Identifier:: 1560109

Report Number(s):: LLNL-JRNL-779339
Journal ID: ISSN 2574-0970; 970006

Grant/Contract Number:: AC52-07NA27344

Resource Type:: Accepted Manuscript

Journal Name:: ACS Applied Nano Materials

Additional Journal Information:: Journal Volume: 1; Journal Issue: 2; Journal ID: ISSN 2574-0970

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Liu, Yang, Wang, Kang-Hsin, Chen, Huan-Yuan, Li, Jie-Ren, Laurence, Ted A., Ly, Sonny, Liu, Fu-Tong, and Liu, Gang-Yu. Periodic Arrangement of Lipopolysaccharides Nanostructures Accelerates and Enhances the Maturation Processes of Dendritic Cells.  United States: N. p., 2018. 
        Web.  doi:10.1021/acsanm.7b00254.

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                    Liu, Yang, Wang, Kang-Hsin, Chen, Huan-Yuan, Li, Jie-Ren, Laurence, Ted A., Ly, Sonny, Liu, Fu-Tong, & Liu, Gang-Yu. Periodic Arrangement of Lipopolysaccharides Nanostructures Accelerates and Enhances the Maturation Processes of Dendritic Cells.  United States.  doi:10.1021/acsanm.7b00254.

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                    Liu, Yang, Wang, Kang-Hsin, Chen, Huan-Yuan, Li, Jie-Ren, Laurence, Ted A., Ly, Sonny, Liu, Fu-Tong, and Liu, Gang-Yu. Mon .  
        "Periodic Arrangement of Lipopolysaccharides Nanostructures Accelerates and Enhances the Maturation Processes of Dendritic Cells".  United States.  doi:10.1021/acsanm.7b00254.  https://www.osti.gov/servlets/purl/1560109.

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@article{osti_1560109,

  title        = {Periodic Arrangement of Lipopolysaccharides Nanostructures Accelerates and Enhances the Maturation Processes of Dendritic Cells},

  author       = {Liu, Yang and Wang, Kang-Hsin and Chen, Huan-Yuan and Li, Jie-Ren and Laurence, Ted A. and Ly, Sonny and Liu, Fu-Tong and Liu, Gang-Yu},

  abstractNote = {This work reports an important application of nanomaterials consisting of biological ligands, such as lipopolysaccharides (LPS) nanostructures. Upon immobilization onto surfaces as periodic arrays of nanostructures, these surface-bound LPS nanostructures significantly impacted the maturation process of bone marrow derived dendritic cells (BMDCs): accelerating the maturation process in comparison to other means of ligand presentations such as LPS solution and monolayers on surfaces. In some cases, LPS nanostructures led to a new maturation phenotype in vitro characterized by hyper-dendritized morphology. This level of maturation enhancement was observed for the first time in vitro. Possible mechanisms leading to the acceleration and enhancement of BMDC maturation were also discussed. This work demonstrates an important concept: surface-bound nanostructures of ligands provide a new and powerful cue for regulating cellular signaling processes. The fact that primary cells’ signaling processes, such as BMDC maturation, can be altered suggests a new means for programming dendritic cells for immune therapy.},

  doi          = {10.1021/acsanm.7b00254},

  journal      = {ACS Applied Nano Materials},

  number       = 2,

  volume       = 1,

  place        = {United States},

  year         = {2018},

  month        = {1}

}

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DOI: 10.1021/acsanm.7b00254

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