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Title: Oral microbiota of periodontal health and disease and their changes after nonsurgical periodontal therapy

Abstract

This study examined the microbial diversity and community assembly of oral microbiota in periodontal health and disease and after nonsurgical periodontal treatment. The V4 region of 16S rRNA gene from DNA of 238 saliva and subgingival samples of 21 healthy and 48 diseased subjects was amplified and sequenced. Among 1979 OTUs identified, 28 were overabundant in diseased plaque. Six of these taxa were also overabundant in diseased saliva. Twelve OTUs were overabundant in healthy plaque. There was a trend for disease-associated taxa to decrease and health-associated taxa to increase after treatment with notable variations among individual sites. Network analysis revealed modularity of the microbial communities and identified several health- and disease-specific modules. Ecological drift was a major factor that governed community turnovers in both plaque and saliva. Dispersal limitation and homogeneous selection affected the community assembly in plaque, with the additional contribution of homogenizing dispersal for plaque within individuals. Homogeneous selection and dispersal limitation played important roles, respectively, in healthy saliva and diseased pre-treatment saliva between individuals. Our results revealed distinctions in both taxa and assembly processes of oral microbiota between periodontal health and disease. Furthermore, the community assembly analysis has identified potentially effective approaches for managing periodontitis.

Authors:
 [1];  [2];  [1];  [3];  [3];  [3];  [3];  [1];  [3];  [3];  [4]
  1. Univ. of Southern California, Los Angeles, CA (United States)
  2. Univ. of Southern California, Los Angeles, CA (United States); Univ. of Oklahoma, Norman, OK (United States); Univ. of Rhode Island, Kingston, RI (United States)
  3. Univ. of Oklahoma, Norman, OK (United States)
  4. Univ. of Oklahoma, Norman, OK (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Tsinghua Univ., Beijing (China)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1559791
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
The ISME Journal
Additional Journal Information:
Journal Volume: 12; Journal Issue: 5; Journal ID: ISSN 1751-7362
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chen, Casey, Hemme, Chris, Beleno, Joan, Shi, Zhou Jason, Ning, Daliang, Qin, Yujia, Tu, Qichao, Jorgensen, Michael, He, Zhili, Wu, Liyou, and Zhou, Jizhong. Oral microbiota of periodontal health and disease and their changes after nonsurgical periodontal therapy. United States: N. p., 2018. Web. doi:10.1038/s41396-017-0037-1.
Chen, Casey, Hemme, Chris, Beleno, Joan, Shi, Zhou Jason, Ning, Daliang, Qin, Yujia, Tu, Qichao, Jorgensen, Michael, He, Zhili, Wu, Liyou, & Zhou, Jizhong. Oral microbiota of periodontal health and disease and their changes after nonsurgical periodontal therapy. United States. doi:10.1038/s41396-017-0037-1.
Chen, Casey, Hemme, Chris, Beleno, Joan, Shi, Zhou Jason, Ning, Daliang, Qin, Yujia, Tu, Qichao, Jorgensen, Michael, He, Zhili, Wu, Liyou, and Zhou, Jizhong. Tue . "Oral microbiota of periodontal health and disease and their changes after nonsurgical periodontal therapy". United States. doi:10.1038/s41396-017-0037-1. https://www.osti.gov/servlets/purl/1559791.
@article{osti_1559791,
title = {Oral microbiota of periodontal health and disease and their changes after nonsurgical periodontal therapy},
author = {Chen, Casey and Hemme, Chris and Beleno, Joan and Shi, Zhou Jason and Ning, Daliang and Qin, Yujia and Tu, Qichao and Jorgensen, Michael and He, Zhili and Wu, Liyou and Zhou, Jizhong},
abstractNote = {This study examined the microbial diversity and community assembly of oral microbiota in periodontal health and disease and after nonsurgical periodontal treatment. The V4 region of 16S rRNA gene from DNA of 238 saliva and subgingival samples of 21 healthy and 48 diseased subjects was amplified and sequenced. Among 1979 OTUs identified, 28 were overabundant in diseased plaque. Six of these taxa were also overabundant in diseased saliva. Twelve OTUs were overabundant in healthy plaque. There was a trend for disease-associated taxa to decrease and health-associated taxa to increase after treatment with notable variations among individual sites. Network analysis revealed modularity of the microbial communities and identified several health- and disease-specific modules. Ecological drift was a major factor that governed community turnovers in both plaque and saliva. Dispersal limitation and homogeneous selection affected the community assembly in plaque, with the additional contribution of homogenizing dispersal for plaque within individuals. Homogeneous selection and dispersal limitation played important roles, respectively, in healthy saliva and diseased pre-treatment saliva between individuals. Our results revealed distinctions in both taxa and assembly processes of oral microbiota between periodontal health and disease. Furthermore, the community assembly analysis has identified potentially effective approaches for managing periodontitis.},
doi = {10.1038/s41396-017-0037-1},
journal = {The ISME Journal},
number = 5,
volume = 12,
place = {United States},
year = {2018},
month = {1}
}

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Works referenced in this record:

Microbial complexes in subgingival plaque
journal, February 1998