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Title: Microbial and metabolic succession on common building materials under high humidity conditions

Abstract

Despite considerable efforts to characterize the microbial ecology of the built environment, the metabolic mechanisms underpinning microbial colonization and successional dynamics remain unclear, particularly at high moisture conditions. Here, we applied bacterial/viral particle counting, qPCR, amplicon sequencing of the genes encoding 16S and ITS rRNA, and metabolomics to longitudinally characterize the ecological dynamics of four common building materials maintained at high humidity. We varied the natural inoculum provided to each material and wet half of the samples to simulate a potable water leak. Wetted materials had higher growth rates and lower alpha diversity compared to non-wetted materials, and wetting described the majority of the variance in bacterial, fungal, and metabolite structure. Inoculation location was weakly associated with bacterial and fungal beta diversity. Material type influenced bacterial and viral particle abundance and bacterial and metabolic (but not fungal) diversity. Metabolites indicative of microbial activity were identified, and they too differed by material.

Authors:
 [1]; ORCiD logo [2];  [3]; ORCiD logo [4]; ORCiD logo [5];  [4];  [6];  [4];  [7]; ORCiD logo [4];  [5];  [3]; ORCiD logo [6]
  1. Univ. of Chicago, IL (United States); Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
  2. Univ. of Chicago, IL (United States)
  3. Illinois Inst. of Technology, Chicago, IL (United States)
  4. Northwestern Univ., Evanston, IL (United States)
  5. San Diego State Univ., CA (United States)
  6. Univ. of California, San Diego, CA (United States)
  7. Argonne National Lab. (ANL), Lemont, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
Alfred P. Sloan Foundation; USDOE
OSTI Identifier:
1559538
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lax, Simon, Cardona, Cesar, Zhao, Dan, Winton, Valerie J., Goodney, Gabriel, Gao, Peng, Gottel, Neil, Hartmann, Erica M., Henry, Chris, Thomas, Paul M., Kelley, Scott T., Stephens, Brent, and Gilbert, Jack A. Microbial and metabolic succession on common building materials under high humidity conditions. United States: N. p., 2019. Web. doi:10.1038/s41467-019-09764-z.
Lax, Simon, Cardona, Cesar, Zhao, Dan, Winton, Valerie J., Goodney, Gabriel, Gao, Peng, Gottel, Neil, Hartmann, Erica M., Henry, Chris, Thomas, Paul M., Kelley, Scott T., Stephens, Brent, & Gilbert, Jack A. Microbial and metabolic succession on common building materials under high humidity conditions. United States. doi:10.1038/s41467-019-09764-z.
Lax, Simon, Cardona, Cesar, Zhao, Dan, Winton, Valerie J., Goodney, Gabriel, Gao, Peng, Gottel, Neil, Hartmann, Erica M., Henry, Chris, Thomas, Paul M., Kelley, Scott T., Stephens, Brent, and Gilbert, Jack A. Tue . "Microbial and metabolic succession on common building materials under high humidity conditions". United States. doi:10.1038/s41467-019-09764-z. https://www.osti.gov/servlets/purl/1559538.
@article{osti_1559538,
title = {Microbial and metabolic succession on common building materials under high humidity conditions},
author = {Lax, Simon and Cardona, Cesar and Zhao, Dan and Winton, Valerie J. and Goodney, Gabriel and Gao, Peng and Gottel, Neil and Hartmann, Erica M. and Henry, Chris and Thomas, Paul M. and Kelley, Scott T. and Stephens, Brent and Gilbert, Jack A.},
abstractNote = {Despite considerable efforts to characterize the microbial ecology of the built environment, the metabolic mechanisms underpinning microbial colonization and successional dynamics remain unclear, particularly at high moisture conditions. Here, we applied bacterial/viral particle counting, qPCR, amplicon sequencing of the genes encoding 16S and ITS rRNA, and metabolomics to longitudinally characterize the ecological dynamics of four common building materials maintained at high humidity. We varied the natural inoculum provided to each material and wet half of the samples to simulate a potable water leak. Wetted materials had higher growth rates and lower alpha diversity compared to non-wetted materials, and wetting described the majority of the variance in bacterial, fungal, and metabolite structure. Inoculation location was weakly associated with bacterial and fungal beta diversity. Material type influenced bacterial and viral particle abundance and bacterial and metabolic (but not fungal) diversity. Metabolites indicative of microbial activity were identified, and they too differed by material.},
doi = {10.1038/s41467-019-09764-z},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {2019},
month = {4}
}

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