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Title: Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows

Abstract

Mitochondrial ATP synthases form dimers, which assemble into long ribbons at the rims of the inner membrane cristae. We reconstituted detergent-purified mitochondrial ATP synthase dimers from the green algae Polytomella sp. and the yeast Yarrowia lipolytica into liposomes and examined them by electron cryotomography. Tomographic volumes revealed that ATP synthase dimers from both species self-assemble into rows and bend the lipid bilayer locally. The dimer rows and the induced degree of membrane curvature closely resemble those in the inner membrane cristae. Monomers of mitochondrial ATP synthase reconstituted into liposomes do not bend membrane visibly and do not form rows. No specific lipids or proteins other than ATP synthase dimers are required for row formation and membrane remodelling. Long rows of ATP synthase dimers are a conserved feature of mitochondrial inner membranes. They are required for cristae formation and a main factor in mitochondrial morphogenesis.

Authors:
 [1];  [1]; ORCiD logo [1];  [1]; ORCiD logo [1]
  1. Max Planck Institute of Biophysics, Frankfurt (Germany)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1559185
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 116; Journal Issue: 10; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; mitochondria; ATP synthase; membrane curvature; electron cryotomography; subtomogram averaging

Citation Formats

Blum, Thorsten B., Hahn, Alexander, Meier, Thomas, Davies, Karen M., and Kühlbrandt, Werner. Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows. United States: N. p., 2019. Web. doi:10.1073/pnas.1816556116.
Blum, Thorsten B., Hahn, Alexander, Meier, Thomas, Davies, Karen M., & Kühlbrandt, Werner. Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows. United States. doi:10.1073/pnas.1816556116.
Blum, Thorsten B., Hahn, Alexander, Meier, Thomas, Davies, Karen M., and Kühlbrandt, Werner. Wed . "Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows". United States. doi:10.1073/pnas.1816556116. https://www.osti.gov/servlets/purl/1559185.
@article{osti_1559185,
title = {Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows},
author = {Blum, Thorsten B. and Hahn, Alexander and Meier, Thomas and Davies, Karen M. and Kühlbrandt, Werner},
abstractNote = {Mitochondrial ATP synthases form dimers, which assemble into long ribbons at the rims of the inner membrane cristae. We reconstituted detergent-purified mitochondrial ATP synthase dimers from the green algae Polytomella sp. and the yeast Yarrowia lipolytica into liposomes and examined them by electron cryotomography. Tomographic volumes revealed that ATP synthase dimers from both species self-assemble into rows and bend the lipid bilayer locally. The dimer rows and the induced degree of membrane curvature closely resemble those in the inner membrane cristae. Monomers of mitochondrial ATP synthase reconstituted into liposomes do not bend membrane visibly and do not form rows. No specific lipids or proteins other than ATP synthase dimers are required for row formation and membrane remodelling. Long rows of ATP synthase dimers are a conserved feature of mitochondrial inner membranes. They are required for cristae formation and a main factor in mitochondrial morphogenesis.},
doi = {10.1073/pnas.1816556116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 10,
volume = 116,
place = {United States},
year = {2019},
month = {2}
}

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Cited by: 13 works
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