Anomalous X-ray diffraction studies of ion transport in K+ channels

Langan, Patricia S.; Vandavasi, Venu Gopal; Weiss, Kevin L.; Afonine, Pavel V.; el Omari, Kamel; Duman, Ramona; Wagner, Armin; Coates, Leighton

doi:10.1038/s41467-018-06957-w

Title: Anomalous X-ray diffraction studies of ion transport in K⁺ channels

Journal Article · 30 October 2018 · Nature Communications

DOI: https://doi.org/10.1038/s41467-018-06957-w · OSTI ID:1559162

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^[1]; Afonine, Pavel V. ^[2];

^[3]; Duman, Ramona ^[3];

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Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Shanghai Univ. (China)
Diamond Light Source, Harwell Science and Innovation Campus, Didcot (United Kingdom)

Potassium ion channels utilize a highly selective filter to rapidly transport K⁺ ions across cellular membranes. This selectivity filter is composed of four binding sites which display almost equal electron density in crystal structures with high potassium ion concentrations. This electron density can be interpreted to reflect a superposition of alternating potassium ion and water occupied states or as adjacent potassium ions. Here, we use single wavelength anomalous dispersion (SAD) X-ray diffraction data collected near the potassium absorption edge to show experimentally that all ion binding sites within the selectivity filter are fully occupied by K⁺ ions. These data support the hypothesis that potassium ion transport occurs by direct Coulomb knock-on, and provide an example of solving the phase problem by K-SAD.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1559162

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 9; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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ANODE : anomalous and heavy-atom density calculation Thorn, Andrea; Sheldrick, George M. Journal of Applied Crystallography, Vol. 44, Issue 6 https://doi.org/10.1107/S0021889811041768	journal	November 2011
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Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
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A shared vision for macromolecular crystallography over the next five years Förster, Andreas; Schulze-Briese, Clemens Structural Dynamics, Vol. 6, Issue 6 https://doi.org/10.1063/1.5131017	journal	November 2019
Conduction through a narrow inward-rectifier K ⁺ channel pore Bernsteiner, Harald; Zangerl-Plessl, Eva-Maria; Chen, Xingyu The Journal of General Physiology, Vol. 151, Issue 10 https://doi.org/10.1085/jgp.201912359	journal	September 2019
Atomistic basis of opening and conduction in mammalian inward rectifier potassium (Kir2.2) channels Zangerl-Plessl, Eva-Maria; Lee, Sun-Joo; Maksaev, Grigory The Journal of General Physiology https://doi.org/10.1085/jgp.201912422	journal	November 2019
Crystallization of a potassium ion channel and X-ray and neutron data collection Langan, Patricia S.; Vandavasi, Venu Gopal; Sullivan, Brendan Acta Crystallographica Section F Structural Biology Communications, Vol. 75, Issue 6 https://doi.org/10.1107/s2053230x19006630	journal	May 2019
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