CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes
Abstract
In recent years CRISPR-Cas technologies have revolutionized microbial engineering approaches. Genome editing and non-editing applications of various CRISPR-Cas systems have expanded the throughput and scale of engineering efforts, as well as opened up new avenues for manipulating genomes of non-model organisms. As we expand the range of organisms used for biotechnological applications, we need to develop better, more versatile tools for manipulation of these systems. Here the authors summarize the current advances in microbial gene editing using CRISPR-Cas based tools and highlight state-of-the-art methods for high-throughput, efficient genome-scale engineering in model organisms Escherichia coli and Saccharomyces cerevisiae. The authors also review non-editing CRISPR-Cas applications available for gene expression manipulation, epigenetic remodeling, RNA editing, labeling, and synthetic gene circuit design. Finally, the authors point out the areas of research that need further development in order to expand the range of applications and increase the utility of these new methods.
- Authors:
-
- Univ. of Colorado, Boulder, CO (United States)
- Univ. of Colorado, Boulder, CO (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1458905
- Alternate Identifier(s):
- OSTI ID: 1557854
- Report Number(s):
- NREL/JA-2700-71862
Journal ID: ISSN 1860-6768
- Grant/Contract Number:
- AC36-08GO28308; SC0018368
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology Journal
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 9; Journal ID: ISSN 1860-6768
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; CRISPR editing; CRISPR gene regulation; CRISPR tools for microbes; CRISPRa; CRISPRi
Citation Formats
Tarasava, Katia, Oh, Eun Joong, Eckert, Carrie A., and Gill, Ryan T. CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes. United States: N. p., 2018.
Web. doi:10.1002/biot.201700586.
Tarasava, Katia, Oh, Eun Joong, Eckert, Carrie A., & Gill, Ryan T. CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes. United States. https://doi.org/10.1002/biot.201700586
Tarasava, Katia, Oh, Eun Joong, Eckert, Carrie A., and Gill, Ryan T. Tue .
"CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes". United States. https://doi.org/10.1002/biot.201700586. https://www.osti.gov/servlets/purl/1458905.
@article{osti_1458905,
title = {CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes},
author = {Tarasava, Katia and Oh, Eun Joong and Eckert, Carrie A. and Gill, Ryan T.},
abstractNote = {In recent years CRISPR-Cas technologies have revolutionized microbial engineering approaches. Genome editing and non-editing applications of various CRISPR-Cas systems have expanded the throughput and scale of engineering efforts, as well as opened up new avenues for manipulating genomes of non-model organisms. As we expand the range of organisms used for biotechnological applications, we need to develop better, more versatile tools for manipulation of these systems. Here the authors summarize the current advances in microbial gene editing using CRISPR-Cas based tools and highlight state-of-the-art methods for high-throughput, efficient genome-scale engineering in model organisms Escherichia coli and Saccharomyces cerevisiae. The authors also review non-editing CRISPR-Cas applications available for gene expression manipulation, epigenetic remodeling, RNA editing, labeling, and synthetic gene circuit design. Finally, the authors point out the areas of research that need further development in order to expand the range of applications and increase the utility of these new methods.},
doi = {10.1002/biot.201700586},
journal = {Biotechnology Journal},
number = 9,
volume = 13,
place = {United States},
year = {Tue Jun 19 00:00:00 EDT 2018},
month = {Tue Jun 19 00:00:00 EDT 2018}
}
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Cited by: 17 works
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Figures / Tables:
Table 1.: CRISPR-enabled genome editing in non-model microbes.
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Works referencing / citing this record:
Developing a CRISPR/Cas9 System for Genome Editing in the Basidiomycetous Yeast Rhodosporidium toruloides
journal, May 2019
- Jiao, Xiang; Zhang, Yue; Liu, Xiangjian
- Biotechnology Journal, Vol. 14, Issue 7
A CRISPR/Cas9 method to generate heterozygous alleles in Saccharomyces cerevisiae
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- EauClaire, Steven F.; Webb, Christopher J.
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CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals
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CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice
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CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice
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Challenges and tackles in metabolic engineering for microbial production of carotenoids
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Figures / Tables found in this record:
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