Vampirovibrio chlorellavorus draft genome sequence, annotation, and preliminary characterization of pathogenicity determinants
Abstract
Vampirovibrio chlorellavorus is recognized as a pathogen of commercially-relevant Chlorella species. Algal infection and total loss of productivity (biomass) often occurs when susceptible algal hosts are cultivated in outdoor open pond systems. The pathogenic life cycle of this bacterium has been inferred from laboratory and field observations, and corroborated in part by the genomic analyses for two Arizona isolates recovered from an open algal reactor. V. chlorellavorus predation has been reported to occur in geographically-and environmentally-diverse conditions. Genomic analyses of these and additional field isolates is expected to reveal new information about the extent of ecological diversity and genes involved in host-pathogen interactions. The draft genome sequences for two isolates of the predatory V. chlorellavorus (Cyanobacteria; Ca. Melainabacteria) from an outdoor cultivation system located in the Arizona Sonoran Desert were assembled and annotated. The genomes were sequenced and analyzed to identify genes (proteins) with predicted involvement in predation, infection, and cell death of Chlorella host species prioritized for biofuel production at sites identified as highly suitable for algal production in the southwestern USA. Genomic analyses identified several predicted genes encoding secreted proteins that are potentially involved in pathogenicity, and at least three apparently complete sets of virulence (Vir) genes, characteristicmore »
- Authors:
-
- Bioscience Division, Los Alamos National Laboratory Los Alamos New Mexico USA
- School of Plant SciencesThe University of Arizona Tucson Arizona USA
- Publication Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Univ. of Arizona, Tucson, AZ (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1592174
- Alternate Identifier(s):
- OSTI ID: 1557759; OSTI ID: 1592175; OSTI ID: 1592757
- Report Number(s):
- LA-UR-19-20027
Journal ID: ISSN 1322-0829
- Grant/Contract Number:
- 89233218CNA000001; EE0006269; NL0029949
- Resource Type:
- Published Article
- Journal Name:
- Phycological Research
- Additional Journal Information:
- Journal Name: Phycological Research Journal Volume: 68 Journal Issue: 1; Journal ID: ISSN 1322-0829
- Publisher:
- Wiley
- Country of Publication:
- Australia
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Biological Science; Algae pathogens; algal pathogen; chlorella; melainabacteria; VirB
Citation Formats
Hovde, Blake T., Steichen, Seth A., Starkenburg, Shawn R., and Brown, Judith K. Vampirovibrio chlorellavorus draft genome sequence, annotation, and preliminary characterization of pathogenicity determinants. Australia: N. p., 2019.
Web. doi:10.1111/pre.12392.
Hovde, Blake T., Steichen, Seth A., Starkenburg, Shawn R., & Brown, Judith K. Vampirovibrio chlorellavorus draft genome sequence, annotation, and preliminary characterization of pathogenicity determinants. Australia. https://doi.org/10.1111/pre.12392
Hovde, Blake T., Steichen, Seth A., Starkenburg, Shawn R., and Brown, Judith K. Wed .
"Vampirovibrio chlorellavorus draft genome sequence, annotation, and preliminary characterization of pathogenicity determinants". Australia. https://doi.org/10.1111/pre.12392.
@article{osti_1592174,
title = {Vampirovibrio chlorellavorus draft genome sequence, annotation, and preliminary characterization of pathogenicity determinants},
author = {Hovde, Blake T. and Steichen, Seth A. and Starkenburg, Shawn R. and Brown, Judith K.},
abstractNote = {Vampirovibrio chlorellavorus is recognized as a pathogen of commercially-relevant Chlorella species. Algal infection and total loss of productivity (biomass) often occurs when susceptible algal hosts are cultivated in outdoor open pond systems. The pathogenic life cycle of this bacterium has been inferred from laboratory and field observations, and corroborated in part by the genomic analyses for two Arizona isolates recovered from an open algal reactor. V. chlorellavorus predation has been reported to occur in geographically-and environmentally-diverse conditions. Genomic analyses of these and additional field isolates is expected to reveal new information about the extent of ecological diversity and genes involved in host-pathogen interactions. The draft genome sequences for two isolates of the predatory V. chlorellavorus (Cyanobacteria; Ca. Melainabacteria) from an outdoor cultivation system located in the Arizona Sonoran Desert were assembled and annotated. The genomes were sequenced and analyzed to identify genes (proteins) with predicted involvement in predation, infection, and cell death of Chlorella host species prioritized for biofuel production at sites identified as highly suitable for algal production in the southwestern USA. Genomic analyses identified several predicted genes encoding secreted proteins that are potentially involved in pathogenicity, and at least three apparently complete sets of virulence (Vir) genes, characteristic of the VirB-VirD type system encoding the canonical VirB1-11 and VirD4 proteins, respectively. Additional protein functions were predicted suggesting their involvement in quorum sensing and motility. The genomes of two previously uncharacterized V. chlorellavorus isolates reveal nucleotide and protein level divergence between each other, and a previously sequenced V. chlorellavorus genome. This new knowledge will enhance the fundamental understanding of trans-kingdom interactions between a unique cosmopolitan cyanobacterial pathogen and its green microalgal host, of broad interest as a source of harvestable biomass for biofuels or bioproducts.},
doi = {10.1111/pre.12392},
journal = {Phycological Research},
number = 1,
volume = 68,
place = {Australia},
year = {Wed Jul 17 00:00:00 EDT 2019},
month = {Wed Jul 17 00:00:00 EDT 2019}
}
https://doi.org/10.1111/pre.12392
Web of Science
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