skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Temperature dependence of nanoscale dynamic processes measured in living millipedes by high resolution inelastic and elastic neutron scattering

Abstract

We have used high energy-resolution neutron scattering to probe nanoscale dynamic processes in living millipedes (Narceus americanus). We have measured the temperature dependence of the intensity of scattered neutrons that do not exchange energy with the living samples on the 1.5 ns time scale, thereby excluding the signal from the highly mobile intra- and extra-cellular bulk-like aqueous constituents in the sample. This measured “elastic” scattering intensity reflects a non-monotonic temperature dependence, with a noticeable systematic decrease detected between 295 and 303 K on warming up from 283 to 310 K. This decrease demonstrates an excellent inverse correlation with the non-monotonic, as a function of temperature, increase in the slow diffusivity previously observed in planarian flatworms and housefly larvae. This correlation indicates the existence of a biological mechanism, possibly common between different classes (Insects and Myriapods) and even phyla (Arthropods and Platyhelminthes), that dampens the slow nanoscopic dynamics in ectothermic organisms in response to the temperature of the environment exceeding the physiologically optimal range.

Authors:
 [1];  [1];  [2]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States); Univ. of Maryland, Gaithersburg, MD (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Scientific User Facilities Division
OSTI Identifier:
1557506
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Mamontov, Eugene, Osti, Naresh C., and Tyagi, Madhusudan. Temperature dependence of nanoscale dynamic processes measured in living millipedes by high resolution inelastic and elastic neutron scattering. United States: N. p., 2019. Web. doi:10.1038/s41598-019-48270-6.
Mamontov, Eugene, Osti, Naresh C., & Tyagi, Madhusudan. Temperature dependence of nanoscale dynamic processes measured in living millipedes by high resolution inelastic and elastic neutron scattering. United States. doi:10.1038/s41598-019-48270-6.
Mamontov, Eugene, Osti, Naresh C., and Tyagi, Madhusudan. Mon . "Temperature dependence of nanoscale dynamic processes measured in living millipedes by high resolution inelastic and elastic neutron scattering". United States. doi:10.1038/s41598-019-48270-6. https://www.osti.gov/servlets/purl/1557506.
@article{osti_1557506,
title = {Temperature dependence of nanoscale dynamic processes measured in living millipedes by high resolution inelastic and elastic neutron scattering},
author = {Mamontov, Eugene and Osti, Naresh C. and Tyagi, Madhusudan},
abstractNote = {We have used high energy-resolution neutron scattering to probe nanoscale dynamic processes in living millipedes (Narceus americanus). We have measured the temperature dependence of the intensity of scattered neutrons that do not exchange energy with the living samples on the 1.5 ns time scale, thereby excluding the signal from the highly mobile intra- and extra-cellular bulk-like aqueous constituents in the sample. This measured “elastic” scattering intensity reflects a non-monotonic temperature dependence, with a noticeable systematic decrease detected between 295 and 303 K on warming up from 283 to 310 K. This decrease demonstrates an excellent inverse correlation with the non-monotonic, as a function of temperature, increase in the slow diffusivity previously observed in planarian flatworms and housefly larvae. This correlation indicates the existence of a biological mechanism, possibly common between different classes (Insects and Myriapods) and even phyla (Arthropods and Platyhelminthes), that dampens the slow nanoscopic dynamics in ectothermic organisms in response to the temperature of the environment exceeding the physiologically optimal range.},
doi = {10.1038/s41598-019-48270-6},
journal = {Scientific Reports},
number = 1,
volume = 9,
place = {United States},
year = {2019},
month = {8}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share:

Works referenced in this record:

Diffusive properties of water in Artemia cysts as determined from quasi-elastic neutron scattering spectra
journal, May 1984


Inelastic Incoherent Neutron Scattering Measurements of Intact Cells and Tissues and Detection of Interfacial Water
journal, April 2004

  • Ford, Robert C.; Ruffle, Stuart V.; Ramirez-Cuesta, Anibal J.
  • Journal of the American Chemical Society, Vol. 126, Issue 14
  • DOI: 10.1021/ja0393269

Picosecond dynamics in haemoglobin from different species: A quasielastic neutron scattering study
journal, October 2014

  • Stadler, Andreas M.; Garvey, Christopher J.; Embs, Jan Peter
  • Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1840, Issue 10
  • DOI: 10.1016/j.bbagen.2014.06.007

Water Dynamics in Neural Tissue
journal, January 2013

  • Natali, Francesca; Dolce, Caterina; Peters, Judith
  • Journal of the Physical Society of Japan, Vol. 82, Issue Suppl.A
  • DOI: 10.7566/JPSJS.82SA.SA017

Mantid—Data analysis and visualization package for neutron scattering and μ SR experiments
journal, November 2014

  • Arnold, O.; Bilheux, J. C.; Borreguero, J. M.
  • Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 764
  • DOI: 10.1016/j.nima.2014.07.029

Down to atomic-scale intracellular water dynamics
journal, May 2008

  • Jasnin, Marion; Moulin, Martina; Haertlein, Michael
  • The EMBO Journal, Vol. 9, Issue 6
  • DOI: 10.1038/embor.2008.50

Infrared spectroscopy as a new tool for studying single living cells: Is there a niche?
journal, December 2017

  • Sabbatini, Simona; Conti, Carla; Orilisi, Giulia
  • Biomedical Spectroscopy and Imaging, Vol. 6, Issue 3-4
  • DOI: 10.3233/BSI-170171

In Vivo Measurement of Internal and Global Macromolecular Motions in Escherichia coli
journal, July 2008


Microscopic diffusion in hydrated encysted eggs of brine shrimp
journal, September 2017


Thermal fluctuations of haemoglobin from different species: adaptation to temperature via conformational dynamics
journal, June 2012

  • Stadler, A. M.; Garvey, C. J.; Bocahut, A.
  • Journal of The Royal Society Interface, Vol. 9, Issue 76
  • DOI: 10.1098/rsif.2012.0364

Organization and Flexibility of Cyanobacterial Thylakoid Membranes Examined by Neutron Scattering
journal, December 2012

  • Liberton, Michelle; Page, Lawrence E.; O'Dell, William B.
  • Journal of Biological Chemistry, Vol. 288, Issue 5
  • DOI: 10.1074/jbc.M112.416933

The high-flux backscattering spectrometer at the NIST Center for Neutron Research
journal, May 2003

  • Meyer, A.; Dimeo, R. M.; Gehring, P. M.
  • Review of Scientific Instruments, Vol. 74, Issue 5
  • DOI: 10.1063/1.1568557

From shell to cell: neutron scattering studies of biological water dynamics and coupling to activity
journal, January 2009

  • Frölich, A.; Gabel, F.; Jasnin, M.
  • Faraday Discuss., Vol. 141
  • DOI: 10.1039/b805506h

Quasi-elastic neutron scattering in the multi-GPa range and its application to liquid water
journal, November 2013

  • Klotz, S.; Strässle, Th.; Bove, L. E.
  • Applied Physics Letters, Vol. 103, Issue 19
  • DOI: 10.1063/1.4828661

Revealing the Dynamics of Thylakoid Membranes in Living Cyanobacterial Cells
journal, January 2016

  • Stingaciu, Laura-Roxana; O’Neill, Hugh; Liberton, Michelle
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep19627

Recent Backscattering Instrument Developments at the ILL and SNS
journal, February 2010

  • Frick, Bernhard; Mamontov, Eugene; Eijck, Lambert van
  • Zeitschrift für Physikalische Chemie, Vol. 224, Issue 1-2
  • DOI: 10.1524/zpch.2010.6091

Anticancer drug impact on DNA – a study by neutron spectroscopy coupled with synchrotron-based FTIR and EXAFS
journal, January 2019

  • Batista de Carvalho, Ana L. M.; Mamede, Adriana P.; Dopplapudi, Asha
  • Physical Chemistry Chemical Physics, Vol. 21, Issue 8
  • DOI: 10.1039/C8CP05881D

Effects of Size and Temperature on Metabolic Rate
journal, September 2001


Feed Conversion, Survival and Development, and Composition of Four Insect Species on Diets Composed of Food By-Products
journal, December 2015


The evolution of thermal physiology in ectotherms
journal, August 2002

  • Angilletta, Michael J.; Niewiarowski, Peter H.; Navas, Carlos A.
  • Journal of Thermal Biology, Vol. 27, Issue 4
  • DOI: 10.1016/S0306-4565(01)00094-8

Non-monotonic temperature dependence of nanoscopic dynamics measured in living housefly larvae
journal, August 2019


Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature
journal, December 2008


Intracellular water – an overlooked drug target? Cisplatin impact in cancer cells probed by neutrons
journal, January 2017

  • Marques, M. P. M.; Batista de Carvalho, A. L. M.; Sakai, V. Garcia
  • Physical Chemistry Chemical Physics, Vol. 19, Issue 4
  • DOI: 10.1039/c6cp05198g

Experimental determination of the nature of diffusive motions of water molecules at low temperatures
journal, March 1985


Deep Sea Microbes Probed by Incoherent Neutron Scattering Under High Hydrostatic Pressure
journal, January 2014

  • Peters, Judith; Martinez, Nicolas; Michoud, Grégoire
  • Zeitschrift für Physikalische Chemie, Vol. 228, Issue 10-12
  • DOI: 10.1515/zpch-2014-0547

In Vivo Protein Dynamics on the Nanometer Length Scale and Nanosecond Time Scale
journal, April 2017

  • Anunciado, Divina B.; Nyugen, Vyncent P.; Hurst, Gregory B.
  • The Journal of Physical Chemistry Letters, Vol. 8, Issue 8
  • DOI: 10.1021/acs.jpclett.7b00399

Specific cellular water dynamics observed in vivo by neutron scattering and NMR
journal, January 2010

  • Jasnin, Marion; Stadler, Andreas; Tehei, Moeava
  • Physical Chemistry Chemical Physics, Vol. 12, Issue 35
  • DOI: 10.1039/c0cp01048k

Macromolecular dynamics in red blood cells investigated using neutron spectroscopy
journal, August 2010

  • Stadler, Andreas Maximilian; van Eijck, Lambert; Demmel, Franz
  • Journal of The Royal Society Interface, Vol. 8, Issue 57
  • DOI: 10.1098/rsif.2010.0306

From Powder to Solution: Hydration Dependence of Human Hemoglobin Dynamics Correlated to Body Temperature
journal, June 2009


Self-diffusion in normal and heavy water in the range 1-45.deg.
journal, March 1973


Intracellular Water Exchange for Measuring the Dry Mass, Water Mass and Changes in Chemical Composition of Living Cells
journal, July 2013


Microscopic diffusion processes measured in living planarians
journal, March 2018


Neutron scattering: a tool to detect in vivo thermal stress effects at the molecular dynamics level in micro-organisms
journal, May 2013

  • Marty, Vincent; Jasnin, Marion; Fabiani, Elisa
  • Journal of The Royal Society Interface, Vol. 10, Issue 82
  • DOI: 10.1098/rsif.2013.0003

Molecular adaptation and salt stress response of Halobacterium salinarum cells revealed by neutron spectroscopy
journal, September 2015


High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes
journal, September 2016

  • Martinez, N.; Michoud, G.; Cario, A.
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep32816

Cytoplasmic Water and Hydration Layer Dynamics in Human Red Blood Cells
journal, December 2008

  • Stadler, Andreas M.; Embs, Jan P.; Digel, Ilya
  • Journal of the American Chemical Society, Vol. 130, Issue 50
  • DOI: 10.1021/ja807691j

A time-of-flight backscattering spectrometer at the Spallation Neutron Source, BASIS
journal, August 2011

  • Mamontov, E.; Herwig, K. W.
  • Review of Scientific Instruments, Vol. 82, Issue 8
  • DOI: 10.1063/1.3626214