Structure of the eukaryotic protein O-mannosyltransferase Pmt1–Pmt2 complex

Bai, Lin; Kovach, Amanda; You, Qinglong; Kenny, Alanna; Li, Huilin

doi:10.1038/s41594-019-0262-6

Title: Structure of the eukaryotic protein O-mannosyltransferase Pmt1–Pmt2 complex

Abstract

In eukaryotes, a nascent peptide entering the endoplasmic reticulum (ER) is scanned by two Sec61 translocon-associated large membrane machines for protein N-glycosylation and protein O-mannosylation, respectively. While the structure of the eight-protein oligosaccharyltransferase complex has been determined recently, the structures of mannosyltransferases of the PMT family, which are an integral part of ER protein homeostasis, are still unknown. In this paper we report cryo-EM structures of the Saccharomyces cerevisiae Pmt1–Pmt2 complex bound to a donor and an acceptor peptide at 3.2-Å resolution, showing that each subunit contains 11 transmembrane helices and a lumenal β-trefoil fold termed the MIR domain. The structures reveal the substrate recognition model and confirm an inverting mannosyl-transferring reaction mechanism by the enzyme complex. Furthermore, we found that the transmembrane domains of Pmt1 and Pmt2 share a structural fold with the catalytic subunits of oligosaccharyltransferases, confirming a previously proposed evolutionary relationship between protein O-mannosylation and protein N-glycosylation.

Authors:

^[1]; Kovach, Amanda ^[1]; You, Qinglong ^[1]; Kenny, Alanna ^[1];

^[1]

Van Andel Research Inst., Grand Rapids, MI (United States)

Publication Date:: 2019-07-08

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Org.:: National Institutes of Health (NIH); Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor

OSTI Identifier:: 1557293

Grant/Contract Number:: R01 CA231466; 085P1000817

Resource Type:: Accepted Manuscript

Journal Name:: Nature Structural & Molecular Biology

Additional Journal Information:: Journal Volume: 26; Journal Issue: 8; Journal ID: ISSN 1545-9993

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: ENGLISH

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Cryoelectron microscopy; Glycobiology; Glycosylation; Transferases

Citation Formats


                    Bai, Lin, Kovach, Amanda, You, Qinglong, Kenny, Alanna, and Li, Huilin. Structure of the eukaryotic protein O-mannosyltransferase Pmt1–Pmt2 complex.  United States: N. p., 2019. 
Web.  https://doi.org/10.1038/s41594-019-0262-6.

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                    Bai, Lin, Kovach, Amanda, You, Qinglong, Kenny, Alanna, & Li, Huilin. Structure of the eukaryotic protein O-mannosyltransferase Pmt1–Pmt2 complex.  United States.  https://doi.org/10.1038/s41594-019-0262-6

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                    Bai, Lin, Kovach, Amanda, You, Qinglong, Kenny, Alanna, and Li, Huilin. Mon .  
"Structure of the eukaryotic protein O-mannosyltransferase Pmt1–Pmt2 complex".  United States.  https://doi.org/10.1038/s41594-019-0262-6.  https://www.osti.gov/servlets/purl/1557293.

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@article{osti_1557293,

  title        = {Structure of the eukaryotic protein O-mannosyltransferase Pmt1–Pmt2 complex},

  author       = {Bai, Lin and Kovach, Amanda and You, Qinglong and Kenny, Alanna and Li, Huilin},

  abstractNote = {In eukaryotes, a nascent peptide entering the endoplasmic reticulum (ER) is scanned by two Sec61 translocon-associated large membrane machines for protein N-glycosylation and protein O-mannosylation, respectively. While the structure of the eight-protein oligosaccharyltransferase complex has been determined recently, the structures of mannosyltransferases of the PMT family, which are an integral part of ER protein homeostasis, are still unknown. In this paper we report cryo-EM structures of the Saccharomyces cerevisiae Pmt1–Pmt2 complex bound to a donor and an acceptor peptide at 3.2-Å resolution, showing that each subunit contains 11 transmembrane helices and a lumenal β-trefoil fold termed the MIR domain. The structures reveal the substrate recognition model and confirm an inverting mannosyl-transferring reaction mechanism by the enzyme complex. Furthermore, we found that the transmembrane domains of Pmt1 and Pmt2 share a structural fold with the catalytic subunits of oligosaccharyltransferases, confirming a previously proposed evolutionary relationship between protein O-mannosylation and protein N-glycosylation.},

  doi          = {10.1038/s41594-019-0262-6},

  journal      = {Nature Structural & Molecular Biology},

  number       = 8,

  volume       = 26,

  place        = {United States},

  year         = {2019},

  month        = {7}

}

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In Saccharomyces cerevisiae, some of the nascent chains can be post-translationally translocated into the endoplasmic reticulum through the heptameric post-translational translocon complex (post-translocon). This membrane-protein complex is composed of the protein-conducting channel and the tetrameric Sec62/63 complex. The Sec62/63 complex plays crucial roles in targeting of the signal recognition particle-independent protein substrate to the protein-conducting channel and in assembly of the post-translocon. Although the molecular mechanism of the post-translational translocation process has been well established, the structure of the post-translocon and how the channel and the Sec62/63 complex form the heptameric complex are largely uncharacterized. Here, we report a 20-{angstrom}more »« less
https://doi.org/10.1074/jbc.M110.159517
The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of the unfolded protein response

Journal Article Zhou, Jiahai ; Liu, Chuan Yin ; Back, Sung Hoon ; ... - Proc. Natl. Acad. Sci. USA

The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I transmembrane ER-localized protein kinase receptors that signal the UPR through a process that involves homodimerization and autophosphorylation. To elucidate the molecular basis of the ER transmembrane signaling event, we determined the x-ray crystal structure of the luminal domain of human IRE1{alpha}. The monomer of the luminal domain comprises a unique fold of a triangular assembly of {beta}-sheet clusters. Structural analysismore »« less
https://doi.org/10.1073/pnas.0606480103
Two alternative binding mechanisms connect the protein translocation Sec71-Sec72 complex with heat shock proteins

Journal Article Tripathi, Arati ; Mandon, Elisabet C. ; Gilmore, Reid ; ... - Journal of Biological Chemistry

The biosynthesis of many eukaryotic proteins requires accurate targeting to and translocation across the endoplasmic reticulum membrane. Post-translational protein translocation in yeast requires both the Sec61 translocation channel, and a complex of four additional proteins: Sec63, Sec62, Sec71, and Sec72. The structure and function of these proteins are largely unknown. This pathway also requires the cytosolic Hsp70 protein Ssa1, but whether Ssa1 associates with the translocation machinery to target protein substrates to the membrane is unclear. Here, we use a combined structural and biochemical approach to explore the role of Sec71-Sec72 subcomplex in post-translational protein translocation. To this end, wemore »« less
https://doi.org/10.1074/jbc.M116.761122
The structure of the PERK kinase domain suggests the mechanism for its activation

Journal Article Cui, Wenjun ; Li, Jingzhi ; Ron, David ; ... - Acta Crystallographica. Section D: Biological Crystallography

The endoplasmic reticulum-localized transmembrane kinase PERK is one of three major ER stress transducers. The crystal structure of PERK’s kinase domain has been determined to 2.8 Å resolution. The endoplasmic reticulum (ER) unfolded protein response (UPR) is comprised of several intracellular signaling pathways that alleviate ER stress. The ER-localized transmembrane kinase PERK is one of three major ER stress transducers. Oligomerization of PERK’s N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr980 on the kinase activation loop. Activated PERK phosphorylates Ser51 of the α-subunit of translation initiation factormore »« less
https://doi.org/10.1107/S0907444911006445

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Title: Structure of the eukaryotic protein O-mannosyltransferase Pmt1–Pmt2 complex

Abstract

Citation Formats

The multiple evolutionary origins of the eukaryotic N-glycosylation pathway journal, August 2016

Molecular basis of lipid-linked oligosaccharide recognition and processing by bacterial oligosaccharyltransferase journal, October 2017

A Conserved Acidic Motif Is Crucial for Enzymatic Activity of Protein O -Mannosyltransferases journal, September 2011

Cardiomyopathy in patients with POMT1-related congenital and limb-girdle muscular dystrophy journal, May 2012

Cryo‐ EM is uncovering the mechanism of eukaryotic protein N‐glycosylation journal, December 2018

Refinement of Macromolecular Structures by the Maximum-Likelihood Method journal, May 1997

Structural basis for dolichylphosphate mannose biosynthesis journal, July 2017

Protein O-mannosylation: Conserved from bacteria to humans journal, May 2009

The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital. journal, November 1996

Dali server update journal, April 2016

O-Mannosylation and human disease journal, November 2012

Membrane protein structural biology in the era of single particle cryo-EM journal, October 2018

Accurate determination of local defocus and specimen tilt in electron microscopy journal, June 2003

Uniparental disomy unveils a novel recessive mutation in POMT2 journal, July 2018

Mechanisms and principles of N-linked protein glycosylation journal, October 2011

A double homozygous mutation in the POMT1 gene involving exon skipping gives rise to Walker-Warburg syndrome in two Spanish Gypsy families journal, July 2009

O-Linked glycosylation in Drosophila melanogaster journal, June 2019

POMT2 gene mutation in limb-girdle muscular dystrophy with inflammatory changes journal, November 2007

An autosomal recessive limb girdle muscular dystrophy (LGMD2) with mild mental retardation is allelic to Walker–Warburg syndrome (WWS) caused by a mutation in the POMT1 gene journal, April 2005

PMT3 andPMT4, two new members of the protein-O-mannosyltransferase gene family ofSaccharomyces cerevisiae journal, November 1995

Structural basis for coupling protein transport and N-glycosylation at the mammalian endoplasmic reticulum journal, March 2018

New tools for automated high-resolution cryo-EM structure determination in RELION-3 journal, November 2018

Recent structures, evolution and mechanisms of glycosyltransferases journal, October 2012

Novel cardiovascular findings in association with a POMT2 mutation: three siblings with α-dystroglycanopathy journal, September 2013

Protein O ‐Mannosylation in Metazoan Organisms journal, February 2014

Mutations in the O-Mannosyltransferase Gene POMT1 Give Rise to the Severe Neuronal Migration Disorder Walker-Warburg Syndrome journal, November 2002

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy journal, February 2017

X-ray structure of a bacterial oligosaccharyltransferase journal, June 2011

UCSF Chimera?A visualization system for exploratory research and analysis journal, January 2004

Genetic alterations of protein-o-mannosyltransferase-1 in glioneuronal and glial brain tumors with subarachnoid spread journal, April 2009

N-linked protein glycosylation in the ER journal, November 2013

The atomic structure of a eukaryotic oligosaccharyltransferase complex journal, January 2018

O -Mannosylation precedes and potentially controls the N -glycosylation of a yeast cell wall glycoprotein journal, June 2003

Detection of variants in dystroglycanopathy-associated genes through the application of targeted whole-exome sequencing analysis to a large cohort of patients with unexplained limb-girdle muscle weakness journal, July 2018

Skeletal muscle MRI of the lower limbs in congenital muscular dystrophy patients with novel POMT1 and POMT2 mutations journal, April 2014

Congenital muscular dystrophies with defective glycosylation of dystroglycan: A population study journal, March 2009

Molecular genetics of the POMT1-related muscular dystrophy-dystroglycanopathies journal, October 2018

Identification of the Lipid Intermediate in Yeast Mannan Biosynthesis journal, August 1973

Noncompaction cardiomyopathy in an infant with Walker-Warburg syndrome journal, October 2017

O-Mannosyl Phosphorylation of Alpha-Dystroglycan Is Required for Laminin Binding journal, December 2009

Analysis of phenotype, enzyme activity and genotype of Chinese patients with POMT1 mutation journal, May 2016

Cystic kidneys in fetal Walker-Warburg syndrome with POMT2 mutation: Intrafamilial phenotypic variability in four siblings and review of literature journal, August 2017

Protein O-mannosylation: What we have learned from baker's yeast journal, November 2013

Dystroglycanopathy with two novel POMT1 mutations in a Chinese boy with developmental delay and muscular dystrophy journal, July 2014

The dolichol pathway of N-linked glycosylation journal, January 1999

Protein O-glycosylation in Saccharomyces cerevisiae Purification and characterization of the dolichyl-phosphate-D-mannose-protein O-D-mannosyltransferase journal, February 1991

Structure-Function Analysis of the Dolichyl Phosphate-Mannose: Protein O -Mannosyltransferase ScPmt1p journal, April 2000

MolProbity : all-atom structure validation for macromolecular crystallography journal, December 2009

Protein O-mannosylation in the early secretory pathway journal, August 2016

Features and development of Coot journal, March 2010

New POMT2 mutations causing congenital muscular dystrophy: Identification of a founder mutation journal, July 2007

POMT1 and POMT2 mutations in CMD patients: A multicentric Italian study journal, July 2008

Catalytic mechanism of enzymic glycosyl transfer journal, November 1990

POMT2 intragenic deletions and splicing abnormalities causing congenital muscular dystrophy with mental retardation journal, July 2009

PHENIX: a comprehensive Python-based system for macromolecular structure solution journal, January 2010

Role of N-glycans in maintaining the activity of protein O-mannosyltransferases POMT1 and POMT2 journal, October 2009

Protein O-mannosyltransferase activities in lymphoblasts from patients with α-dystroglycanopathies journal, January 2008

Topography of Glycosylation in The Rough Endoplasmic Reticulum and Golgi Apparatus journal, June 1987

Advances in understanding glycosyltransferases from a structural perspective journal, October 2014

Glycosyltransferases: Structures, Functions, and Mechanisms journal, June 2008

Futile Protein Folding Cycles in the ER Are Terminated by the Unfolded Protein O-Mannosylation Pathway journal, May 2013

Glycosyl Transfer from Dolichyl Phosphate Sugars to Endogenous and Exogenous Glycoprotein Acceptors in Yeast journal, February 1978

The expanding phenotype of POMT1 mutations: from Walker-Warburg syndrome to congenital muscular dystrophy, microcephaly, and mental retardation journal, May 2006

Dolichol-phosphate mannose synthase: Structure, function and regulation journal, June 2008

A novel POMT2 mutation causes mild congenital muscular dystrophy with normal brain MRI journal, June 2009

Glycosyltransferase structure and mechanism journal, October 2000

Crystal Structure of the Marasmius Oreades Mushroom Lectin in Complex with a Xenotransplantation Epitope journal, June 2007

Protein O -Mannosyltransferases Associate with the Translocon to Modify Translocating Polypeptide Chains journal, February 2014

Mutations inPOMT1 are found in a minority of patients with Walker-Warburg syndrome journal, January 2005

[20] Processing of X-ray diffraction data collected in oscillation mode book, January 1997

Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation journal, October 2013

Structure of the yeast oligosaccharyltransferase complex gives insight into eukaryotic N-glycosylation journal, January 2018

Exploring the gas access routes in a [NiFeSe] hydrogenase using crystals pressurized with krypton and oxygen journal, August 2020

PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome journal, December 2019

Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation journal, February 2021

Detection of variants in dystroglycanopathy-associated genes through the application of targeted whole-exome sequencing analysis to a large cohort of patients with unexplained limb-girdle muscle weakness text, January 2018

Structure of the yeast oligosaccharyltransferase complex gives insight into eukaryotic N-glycosylation text, January 2018

The multiple evolutionary origins of the eukaryotic N-glycosylation pathway
journal, August 2016

Molecular basis of lipid-linked oligosaccharide recognition and processing by bacterial oligosaccharyltransferase
journal, October 2017

A Conserved Acidic Motif Is Crucial for Enzymatic Activity of Protein O -Mannosyltransferases
journal, September 2011

Cardiomyopathy in patients with POMT1-related congenital and limb-girdle muscular dystrophy
journal, May 2012

Cryo‐ EM is uncovering the mechanism of eukaryotic protein N‐glycosylation
journal, December 2018

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

Structural basis for dolichylphosphate mannose biosynthesis
journal, July 2017

Protein O-mannosylation: Conserved from bacteria to humans
journal, May 2009

The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital.
journal, November 1996

Dali server update
journal, April 2016

O-Mannosylation and human disease
journal, November 2012

Membrane protein structural biology in the era of single particle cryo-EM
journal, October 2018

Accurate determination of local defocus and specimen tilt in electron microscopy
journal, June 2003

Uniparental disomy unveils a novel recessive mutation in POMT2
journal, July 2018

Mechanisms and principles of N-linked protein glycosylation
journal, October 2011

A double homozygous mutation in the POMT1 gene involving exon skipping gives rise to Walker-Warburg syndrome in two Spanish Gypsy families
journal, July 2009

O-Linked glycosylation in Drosophila melanogaster
journal, June 2019

POMT2 gene mutation in limb-girdle muscular dystrophy with inflammatory changes
journal, November 2007

An autosomal recessive limb girdle muscular dystrophy (LGMD2) with mild mental retardation is allelic to Walker–Warburg syndrome (WWS) caused by a mutation in the POMT1 gene
journal, April 2005

PMT3 andPMT4, two new members of the protein-O-mannosyltransferase gene family ofSaccharomyces cerevisiae
journal, November 1995

Structural basis for coupling protein transport and N-glycosylation at the mammalian endoplasmic reticulum
journal, March 2018

New tools for automated high-resolution cryo-EM structure determination in RELION-3
journal, November 2018

Recent structures, evolution and mechanisms of glycosyltransferases
journal, October 2012

Novel cardiovascular findings in association with a POMT2 mutation: three siblings with α-dystroglycanopathy
journal, September 2013

Protein O ‐Mannosylation in Metazoan Organisms
journal, February 2014

Mutations in the O-Mannosyltransferase Gene POMT1 Give Rise to the Severe Neuronal Migration Disorder Walker-Warburg Syndrome
journal, November 2002

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy
journal, February 2017

X-ray structure of a bacterial oligosaccharyltransferase
journal, June 2011

UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004

Genetic alterations of protein-o-mannosyltransferase-1 in glioneuronal and glial brain tumors with subarachnoid spread
journal, April 2009

N-linked protein glycosylation in the ER
journal, November 2013

The atomic structure of a eukaryotic oligosaccharyltransferase complex
journal, January 2018

O -Mannosylation precedes and potentially controls the N -glycosylation of a yeast cell wall glycoprotein
journal, June 2003

Detection of variants in dystroglycanopathy-associated genes through the application of targeted whole-exome sequencing analysis to a large cohort of patients with unexplained limb-girdle muscle weakness
journal, July 2018

Skeletal muscle MRI of the lower limbs in congenital muscular dystrophy patients with novel POMT1 and POMT2 mutations
journal, April 2014

Congenital muscular dystrophies with defective glycosylation of dystroglycan: A population study
journal, March 2009

Molecular genetics of the POMT1-related muscular dystrophy-dystroglycanopathies
journal, October 2018

Identification of the Lipid Intermediate in Yeast Mannan Biosynthesis
journal, August 1973

Noncompaction cardiomyopathy in an infant with Walker-Warburg syndrome
journal, October 2017

O-Mannosyl Phosphorylation of Alpha-Dystroglycan Is Required for Laminin Binding
journal, December 2009

Analysis of phenotype, enzyme activity and genotype of Chinese patients with POMT1 mutation
journal, May 2016

Cystic kidneys in fetal Walker-Warburg syndrome with POMT2 mutation: Intrafamilial phenotypic variability in four siblings and review of literature
journal, August 2017

Protein O-mannosylation: What we have learned from baker's yeast
journal, November 2013

Dystroglycanopathy with two novel POMT1 mutations in a Chinese boy with developmental delay and muscular dystrophy
journal, July 2014

The dolichol pathway of N-linked glycosylation
journal, January 1999

Protein O-glycosylation in Saccharomyces cerevisiae Purification and characterization of the dolichyl-phosphate-D-mannose-protein O-D-mannosyltransferase
journal, February 1991

Structure-Function Analysis of the Dolichyl Phosphate-Mannose: Protein O -Mannosyltransferase ScPmt1p
journal, April 2000

MolProbity : all-atom structure validation for macromolecular crystallography
journal, December 2009

Protein O-mannosylation in the early secretory pathway
journal, August 2016

Features and development of Coot
journal, March 2010

New POMT2 mutations causing congenital muscular dystrophy: Identification of a founder mutation
journal, July 2007

POMT1 and POMT2 mutations in CMD patients: A multicentric Italian study
journal, July 2008

Catalytic mechanism of enzymic glycosyl transfer
journal, November 1990

POMT2 intragenic deletions and splicing abnormalities causing congenital muscular dystrophy with mental retardation
journal, July 2009

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

Role of N-glycans in maintaining the activity of protein O-mannosyltransferases POMT1 and POMT2
journal, October 2009

Protein O-mannosyltransferase activities in lymphoblasts from patients with α-dystroglycanopathies
journal, January 2008

Topography of Glycosylation in The Rough Endoplasmic Reticulum and Golgi Apparatus
journal, June 1987

Advances in understanding glycosyltransferases from a structural perspective
journal, October 2014

Glycosyltransferases: Structures, Functions, and Mechanisms
journal, June 2008

Futile Protein Folding Cycles in the ER Are Terminated by the Unfolded Protein O-Mannosylation Pathway
journal, May 2013

Glycosyl Transfer from Dolichyl Phosphate Sugars to Endogenous and Exogenous Glycoprotein Acceptors in Yeast
journal, February 1978

The expanding phenotype of POMT1 mutations: from Walker-Warburg syndrome to congenital muscular dystrophy, microcephaly, and mental retardation
journal, May 2006

Dolichol-phosphate mannose synthase: Structure, function and regulation
journal, June 2008

A novel POMT2 mutation causes mild congenital muscular dystrophy with normal brain MRI
journal, June 2009

Glycosyltransferase structure and mechanism
journal, October 2000

Crystal Structure of the Marasmius Oreades Mushroom Lectin in Complex with a Xenotransplantation Epitope
journal, June 2007

Protein O -Mannosyltransferases Associate with the Translocon to Modify Translocating Polypeptide Chains
journal, February 2014

Mutations inPOMT1 are found in a minority of patients with Walker-Warburg syndrome
journal, January 2005

[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997

Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation
journal, October 2013

Structure of the yeast oligosaccharyltransferase complex gives insight into eukaryotic N-glycosylation
journal, January 2018

Exploring the gas access routes in a [NiFeSe] hydrogenase using crystals pressurized with krypton and oxygen
journal, August 2020

PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome
journal, December 2019

Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation
journal, February 2021

Detection of variants in dystroglycanopathy-associated genes through the application of targeted whole-exome sequencing analysis to a large cohort of patients with unexplained limb-girdle muscle weakness
text, January 2018

Structure of the yeast oligosaccharyltransferase complex gives insight into eukaryotic N-glycosylation
text, January 2018

Cryo–electron microscopy structures of human oligosaccharyltransferase complexes OST-A and OST-B
journal, December 2019

Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily
journal, September 2019

Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria
journal, May 2020