Structure of the eukaryotic protein O-mannosyltransferase Pmt1–Pmt2 complex

Bai, Lin; Kovach, Amanda; You, Qinglong; Kenny, Alanna; Li, Huilin

doi:10.1038/s41594-019-0262-6

Title: Structure of the eukaryotic protein O-mannosyltransferase Pmt1–Pmt2 complex

Journal Article · 07 July 2019 · Nature Structural & Molecular Biology

DOI: https://doi.org/10.1038/s41594-019-0262-6 · OSTI ID:1557293

^[1]; Kovach, Amanda ^[1]; You, Qinglong ^[1]; Kenny, Alanna ^[1];

^[1]

Van Andel Research Inst., Grand Rapids, MI (United States)

In eukaryotes, a nascent peptide entering the endoplasmic reticulum (ER) is scanned by two Sec61 translocon-associated large membrane machines for protein N-glycosylation and protein O-mannosylation, respectively. While the structure of the eight-protein oligosaccharyltransferase complex has been determined recently, the structures of mannosyltransferases of the PMT family, which are an integral part of ER protein homeostasis, are still unknown. In this paper we report cryo-EM structures of the Saccharomyces cerevisiae Pmt1–Pmt2 complex bound to a donor and an acceptor peptide at 3.2-Å resolution, showing that each subunit contains 11 transmembrane helices and a lumenal β-trefoil fold termed the MIR domain. The structures reveal the substrate recognition model and confirm an inverting mannosyl-transferring reaction mechanism by the enzyme complex. Furthermore, we found that the transmembrane domains of Pmt1 and Pmt2 share a structural fold with the catalytic subunits of oligosaccharyltransferases, confirming a previously proposed evolutionary relationship between protein O-mannosylation and protein N-glycosylation.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor

OSTI ID:: 1557293

Journal Information:: Nature Structural & Molecular Biology, Journal Name: Nature Structural & Molecular Biology Journal Issue: 8 Vol. 26; ISSN 1545-9993

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Mutations inPOMT1 are found in a minority of patients with Walker-Warburg syndrome Currier, Sophie C.; Lee, Christine K.; Chang, Bernard S. American Journal of Medical Genetics Part A, Vol. 133A, Issue 1 https://doi.org/10.1002/ajmg.a.30487	journal	January 2005
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