Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2

Klontz, Erik H.; Trastoy, Beatriz; Deredge, Daniel; Fields, James K.; Li, Chao; Orwenyo, Jared; Marina, Alberto; Beadenkopf, Robert; Günther, Sebastian; Flores, Jair; Wintrode, Patrick L.; Wang, Lai-Xi; Guerin, Marcelo E.; Sundberg, Eric J.

doi:10.1021/acscentsci.8b00917

Title: Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2

Journal Article · 06 February 2019 · ACS Central Science

DOI: https://doi.org/10.1021/acscentsci.8b00917 · OSTI ID:1557279

^[1]; Trastoy, Beatriz;

; Fields, James K. ^[1];

; Orwenyo, Jared; Marina, Alberto; Beadenkopf, Robert ^[1]; Günther, Sebastian ^[2]; Flores, Jair ^[1]; Wintrode, Patrick L. ^[3];

^[4];

^[5];

^[1]

Univ. of Maryland School of Medicine, Baltimore, MD (United States)
Univ. of Maryland School of Medicine, Baltimore, MD (United States); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
Univ. of Maryland School of Pharmacy, Baltimore, MD (United States)
Univ. of Maryland, College Park, MD (United States)
CIC bioGUNE, Derio (Spain); Basque Foundation for Science, Bilbao (Spain)

Immunoglobulin G (IgG) glycosylation critically modulates antibody effector functions. Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2, which deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system. EndoS2 and specific point mutants have been used to chemoenzymatically synthesize antibodies with customizable glycosylation for gain of functions. EndoS2 is useful in these schemes because it accommodates a broad range of N-glycans, including high-mannose, complex, and hybrid types; however, its mechanism of substrate recognition is poorly understood. We present crystal structures of EndoS2 alone and bound to complex and high-mannose glycans; the broad N-glycan specificity is governed by critical loops that shape the binding site of EndoS2. Furthermore, hydrolytic experiments, domain-swap chimeras, and hydrogen–deuterium exchange mass spectrometry reveal the importance of the carbohydrate-binding module in the mechanism of IgG recognition by EndoS2, providing insights into engineering enzymes to catalyze customizable glycosylation reactions.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS); SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS); Ministry of Economy and Competitiveness (MINECO); Federación Española de Enfermedades Raras (FEDER)

Grant/Contract Number:: AC02-06CH11357; AC02-76SF00515

OSTI ID:: 1557279

Journal Information:: ACS Central Science, Journal Name: ACS Central Science Journal Issue: 3 Vol. 5; ISSN 2374-7943

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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On enzymatic remodeling of IgG glycosylation; unique tools with broad applications Sjögren, Jonathan; Lood, Rolf; Nägeli, Andreas Glycobiology, Vol. 30, Issue 4 https://doi.org/10.1093/glycob/cwz085	journal	October 2019
Structural basis for the specific cleavage of core-fucosylated N -glycans by endo-β- N -acetylglucosaminidase from the fungus Cordyceps militaris Seki, Haruka; Huang, Yibo; Arakawa, Takatoshi Journal of Biological Chemistry, Vol. 294, Issue 45 https://doi.org/10.1074/jbc.ra119.010842	journal	September 2019
Structural insights into the mechanisms and specificities of IgG-active endoglycosidases Du, Jonathan J.; Klontz, Erik H.; Guerin, Marcelo E. Glycobiology https://doi.org/10.1093/glycob/cwz042	journal	June 2019

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