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Title: A universal discoidal nanoplatform for the intracellular delivery of PNAs

Abstract

Peptide nucleic acids (PNAs) have gained considerable attention due to their remarkable potential in gene editing and targeting-based strategies. However, cellular delivery of PNAs remains a challenge in developing their broader therapeutic applications. Here, we investigated a novel complex made of lipid bicelles and PNA-based carriers for the efficient delivery of PNAs. For proof of concept, PNAs targeting microRNA (miR) 210 and 155 were tested. Comprehensive evaluation of positive as well as negative charge-containing bicelles with PNA : lipid ratios of 1 : 100, 1 : 1000, and 1 : 2500 was performed. The negatively charged bicelles with a PNA : lipid molar ratio of 1 : 2500 yielded a discoidal shape with a uniform diameter of ~30 nm and a bilayer thickness of 5 nm, while the positively charged bicellar system contained irregular vesicles after the incorporation of PNA. Small-angle X-ray scattering (SAXS) analysis was performed to provide insight into how the hydrophobic PNAs interact with bicelles. Further, flow cytometry followed by confocal microscopy analyses substantiate the superior transfection efficiency of bicelles containing dyeconjugated antimiR PNAs. Functional analysis also confirmed miR inhibition by PNA oligomers delivered by bicelles. The nanodiscoidal complex opens a new pathway to deliver PNAs, which,more » on their own, are a great challenge to be endocytosed into cells.« less

Authors:
 [1];  [1];  [2];  [3]; ORCiD logo [1]; ORCiD logo [1]
  1. Univ. of Connecticut, Storrs, CT (United States)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)
  3. Univ. of California, San Diego, CA (United States)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1557110
Alternate Identifier(s):
OSTI ID: 1525915
Report Number(s):
BNL-211968-2019-JAAM
Journal ID: ISSN 2040-3364; NANOHL
Grant/Contract Number:  
SC0012704; KP1605010
Resource Type:
Accepted Manuscript
Journal Name:
Nanoscale
Additional Journal Information:
Journal Volume: 11; Journal Issue: 26; Journal ID: ISSN 2040-3364
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Tahmasbi Rad, Armin, Malik, Shipra, Yang, Lin, Oberoi-Khanuja, Tripat Kaur, Nieh, Mu-Ping, and Bahal, Raman. A universal discoidal nanoplatform for the intracellular delivery of PNAs. United States: N. p., 2019. Web. doi:10.1039/C9NR03667A.
Tahmasbi Rad, Armin, Malik, Shipra, Yang, Lin, Oberoi-Khanuja, Tripat Kaur, Nieh, Mu-Ping, & Bahal, Raman. A universal discoidal nanoplatform for the intracellular delivery of PNAs. United States. doi:10.1039/C9NR03667A.
Tahmasbi Rad, Armin, Malik, Shipra, Yang, Lin, Oberoi-Khanuja, Tripat Kaur, Nieh, Mu-Ping, and Bahal, Raman. Tue . "A universal discoidal nanoplatform for the intracellular delivery of PNAs". United States. doi:10.1039/C9NR03667A.
@article{osti_1557110,
title = {A universal discoidal nanoplatform for the intracellular delivery of PNAs},
author = {Tahmasbi Rad, Armin and Malik, Shipra and Yang, Lin and Oberoi-Khanuja, Tripat Kaur and Nieh, Mu-Ping and Bahal, Raman},
abstractNote = {Peptide nucleic acids (PNAs) have gained considerable attention due to their remarkable potential in gene editing and targeting-based strategies. However, cellular delivery of PNAs remains a challenge in developing their broader therapeutic applications. Here, we investigated a novel complex made of lipid bicelles and PNA-based carriers for the efficient delivery of PNAs. For proof of concept, PNAs targeting microRNA (miR) 210 and 155 were tested. Comprehensive evaluation of positive as well as negative charge-containing bicelles with PNA : lipid ratios of 1 : 100, 1 : 1000, and 1 : 2500 was performed. The negatively charged bicelles with a PNA : lipid molar ratio of 1 : 2500 yielded a discoidal shape with a uniform diameter of ~30 nm and a bilayer thickness of 5 nm, while the positively charged bicellar system contained irregular vesicles after the incorporation of PNA. Small-angle X-ray scattering (SAXS) analysis was performed to provide insight into how the hydrophobic PNAs interact with bicelles. Further, flow cytometry followed by confocal microscopy analyses substantiate the superior transfection efficiency of bicelles containing dyeconjugated antimiR PNAs. Functional analysis also confirmed miR inhibition by PNA oligomers delivered by bicelles. The nanodiscoidal complex opens a new pathway to deliver PNAs, which, on their own, are a great challenge to be endocytosed into cells.},
doi = {10.1039/C9NR03667A},
journal = {Nanoscale},
number = 26,
volume = 11,
place = {United States},
year = {2019},
month = {5}
}

Journal Article:
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Works referenced in this record:

PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules
journal, October 1993

  • Egholm, Michael; Buchardt, Ole; Christensen, Leif
  • Nature, Vol. 365, Issue 6446
  • DOI: 10.1038/365566a0

Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide
journal, December 1991


Stability of peptide nucleic acids in human serum and cellular extracts
journal, September 1994

  • Demidov, Vadim V.; Potaman, Vladimir N.; Frank-Kamenetskil, M. D.
  • Biochemical Pharmacology, Vol. 48, Issue 6
  • DOI: 10.1016/0006-2952(94)90171-6

Nanotechnology for delivery of peptide nucleic acids (PNAs)
journal, October 2016


Nanoparticle for delivery of antisense γPNA oligomers targeting CCR5
journal, April 2013

  • Bahal, Raman; McNeer, Nicole Ali; Ly, Danith H.
  • Artificial DNA: PNA & XNA, Vol. 4, Issue 2
  • DOI: 10.4161/adna.25628

Applications of PNA-laden nanoparticles for hematological disorders
journal, November 2018

  • Malik, Shipra; Oyaghire, Stanley; Bahal, Raman
  • Cellular and Molecular Life Sciences, Vol. 76, Issue 6
  • DOI: 10.1007/s00018-018-2979-5

Advances in Nanoparticle-based Delivery of Next Generation Peptide Nucleic Acids
journal, March 2019



journal, October 2013


Synthesis of Conformationally Preorganized and Cell-Permeable Guanidine-Based γ-Peptide Nucleic Acids (γGPNAs)
journal, February 2009

  • Sahu, Bichismita; Chenna, Venugopal; Lathrop, Kira L.
  • The Journal of Organic Chemistry, Vol. 74, Issue 4
  • DOI: 10.1021/jo802211n

Preparation of avidin-labelled gelatin nanoparticles as carriers for biotinylated peptide nucleic acid (PNA)
journal, March 2000


Intracellular Delivery of Peptide Nucleic Acid and Organic Molecules Using Zeolite-L Nanocrystals
journal, May 2014

  • Bertucci, Alessandro; Lülf, Henning; Septiadi, Dedy
  • Advanced Healthcare Materials, Vol. 3, Issue 11
  • DOI: 10.1002/adhm.201400116

Combined Delivery of Temozolomide and Anti-miR221 PNA Using Mesoporous Silica Nanoparticles Induces Apoptosis in Resistant Glioma Cells
journal, September 2015

  • Bertucci, Alessandro; Prasetyanto, Eko Adi; Septiadi, Dedy
  • Small, Vol. 11, Issue 42
  • DOI: 10.1002/smll.201500540

In vivo correction of anaemia in β-thalassemic mice by γPNA-mediated gene editing with nanoparticle delivery
journal, October 2016

  • Bahal, Raman; Ali McNeer, Nicole; Quijano, Elias
  • Nature Communications, Vol. 7, Issue 1
  • DOI: 10.1038/ncomms13304

In utero nanoparticle delivery for site-specific genome editing
journal, June 2018


Small unilamellar vesicles: a platform technology for molecular imaging of brain tumors
journal, March 2011


Lipid–protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes
journal, March 2012

  • Lyukmanova, E. N.; Shenkarev, Z. O.; Khabibullina, N. F.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1818, Issue 3
  • DOI: 10.1016/j.bbamem.2011.10.020

The effects of temperature, salinity, concentration and PEGylated lipid on the spontaneous nanostructures of bicellar mixtures
journal, July 2014

  • Liu, Ying; Li, Ming; Yang, Yongkun
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1838, Issue 7
  • DOI: 10.1016/j.bbamem.2014.02.004

The Morphology of Self-Assembled Lipid-Based Nanoparticles Affects Their Uptake by Cancer Cells
journal, October 2016

  • Aresh, Wafa; Liu, Ying; Sine, Jessica
  • Journal of Biomedical Nanotechnology, Vol. 12, Issue 10
  • DOI: 10.1166/jbn.2016.2292

Control of Endothelial Targeting and Intracellular Delivery of Therapeutic Enzymes by Modulating the Size and Shape of ICAM-1-targeted Carriers
journal, August 2008

  • Muro, Silvia; Garnacho, Carmen; Champion, Julie A.
  • Molecular Therapy, Vol. 16, Issue 8
  • DOI: 10.1038/mt.2008.127

Principles of nanoparticle design for overcoming biological barriers to drug delivery
journal, September 2015

  • Blanco, Elvin; Shen, Haifa; Ferrari, Mauro
  • Nature Biotechnology, Vol. 33, Issue 9
  • DOI: 10.1038/nbt.3330

Mammalian cells preferentially internalize hydrogel nanodiscs over nanorods and use shape-specific uptake mechanisms
journal, October 2013

  • Agarwal, R.; Singh, V.; Jurney, P.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 43
  • DOI: 10.1073/pnas.1305000110

The influence of size, shape and vessel geometry on nanoparticle distribution
journal, July 2012


Strategies for the intracellular delivery of nanoparticles
journal, January 2011

  • Chou, Leo Y. T.; Ming, Kevin; Chan, Warren C. W.
  • Chem. Soc. Rev., Vol. 40, Issue 1
  • DOI: 10.1039/C0CS00003E

Effect of Shape, Size, and Aspect Ratio on Nanoparticle Penetration and Distribution inside Solid Tissues Using 3D Spheroid Models
journal, September 2015

  • Agarwal, Rachit; Jurney, Patrick; Raythatha, Mansi
  • Advanced Healthcare Materials, Vol. 4, Issue 15
  • DOI: 10.1002/adhm.201500441

Using an in-vacuum CCD detector for simultaneous small- and wide-angle scattering at beamline X9
journal, January 2013


Differential surface properties of commercial crystalline telmisartan samples
journal, May 2013

  • Laad, Preksha; Shete, Ganesh; Modi, Sameer R.
  • European Journal of Pharmaceutical Sciences, Vol. 49, Issue 2
  • DOI: 10.1016/j.ejps.2013.02.017

Solid-Phase synthesis of peptide nucleic acids
journal, May 1995

  • Christensen, Leif; Fitzpatrick, Richard; Gildea, Brian
  • Journal of Peptide Science, Vol. 1, Issue 3
  • DOI: 10.1002/psc.310010304

Anti-tumor Activity of miniPEG-γ-Modified PNAs to Inhibit MicroRNA-210 for Cancer Therapy
journal, December 2017


Nanoparticle-based therapy in an in vivo microRNA-155 (miR-155)-dependent mouse model of lymphoma
journal, June 2012

  • Babar, I. A.; Cheng, C. J.; Booth, C. J.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 26
  • DOI: 10.1073/pnas.1201516109

MicroRNA silencing for cancer therapy targeted to the tumour microenvironment
journal, November 2014

  • Cheng, Christopher J.; Bahal, Raman; Babar, Imran A.
  • Nature, Vol. 518, Issue 7537
  • DOI: 10.1038/nature13905

Guiding the location of nanoparticles into vesicular structures: a morphological study
journal, January 2007

  • Binder, Wolfgang H.; Sachsenhofer, Robert; Farnik, Dominique
  • Physical Chemistry Chemical Physics, Vol. 9, Issue 48
  • DOI: 10.1039/b711470m

Hydrophobic Gold Nanoparticle Self-Assembly with Phosphatidylcholine Lipid: Membrane-Loaded and Janus Vesicles
journal, September 2010

  • Rasch, Michael R.; Rossinyol, Emma; Hueso, Jose L.
  • Nano Letters, Vol. 10, Issue 9
  • DOI: 10.1021/nl102387n

Structural and Thermal Analysis of Lipid Vesicles Encapsulating Hydrophobic Gold Nanoparticles
journal, May 2012

  • Von White, Gregory; Chen, Yanjing; Roder-Hanna, Julia
  • ACS Nano, Vol. 6, Issue 6
  • DOI: 10.1021/nn2042016

Smart Photothermal-Triggered Bilayer Phase Transition in AuNPs–Liposomes to Release Drug
journal, January 2013

  • An, Xueqin; Zhan, Fan; Zhu, Yinyan
  • Langmuir, Vol. 29, Issue 4
  • DOI: 10.1021/la304692h

Lipid tail protrusions mediate the insertion of nanoparticles into model cell membranes
journal, July 2014

  • Van Lehn, Reid C.; Ricci, Maria; Silva, Paulo H. J.
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms5482

Fusion of Ligand-Coated Nanoparticles with Lipid Bilayers: Effect of Ligand Flexibility
journal, May 2014

  • Van Lehn, Reid C.; Alexander-Katz, Alfredo
  • The Journal of Physical Chemistry A, Vol. 118, Issue 31
  • DOI: 10.1021/jp411662c

Pathway for insertion of amphiphilic nanoparticles into defect-free lipid bilayers from atomistic molecular dynamics simulations
journal, January 2015

  • Van Lehn, Reid C.; Alexander-Katz, Alfredo
  • Soft Matter, Vol. 11, Issue 16
  • DOI: 10.1039/C5SM00287G

Effects of Membrane Defects and Polymer Hydrophobicity on Networking Kinetics of Vesicles
journal, May 2017


The Effect of Nanoparticle Size, Shape, and Surface Chemistry on Biological Systems
journal, August 2012


A Truncated HIV-1 Tat Protein Basic Domain Rapidly Translocates through the Plasma Membrane and Accumulates in the Cell Nucleus
journal, June 1997

  • Vivès, Eric; Brodin, Priscille; Lebleu, Bernard
  • Journal of Biological Chemistry, Vol. 272, Issue 25
  • DOI: 10.1074/jbc.272.25.16010

Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
journal, September 2005


Endocytosis of nanomedicines
journal, August 2010


Combining Anti-Mir-155 with Chemotherapy for the Treatment of Lung Cancers
journal, November 2016


Fluorescent Nanorods and Nanospheres for Real-Time In Vivo Probing of Nanoparticle Shape-Dependent Tumor Penetration
journal, October 2011

  • Chauhan, Vikash P.; Popović, Zoran; Chen, Ou
  • Angewandte Chemie International Edition, Vol. 50, Issue 48
  • DOI: 10.1002/anie.201104449

Self-Assembled Porphyrin Nanodiscs with Structure-Dependent Activation for Phototherapy and Photodiagnostic Applications
journal, March 2013

  • Ng, Kenneth K.; Lovell, Jonathan F.; Vedadi, Ali
  • ACS Nano, Vol. 7, Issue 4
  • DOI: 10.1021/nn400418y

The Effects of Particle Size and Molecular Targeting on the Intratumoral and Subcellular Distribution of Polymeric Nanoparticles
journal, May 2010

  • Lee, Helen; Fonge, Humphrey; Hoang, Bryan
  • Molecular Pharmaceutics, Vol. 7, Issue 4
  • DOI: 10.1021/mp100038h

The effect of shape on the margination dynamics of non-neutrally buoyant particles in two-dimensional shear flows
journal, July 2008


Effect of Surface Properties on Nanoparticle–Cell Interactions
journal, January 2010


Lipid Nanodisc-Templated Self-Assembly of Gold Nanoparticles into Strings and Rings
journal, March 2017


    Works referencing / citing this record:

    Intracellular Delivery of Peptide Nucleic Acid and Organic Molecules Using Zeolite-L Nanocrystals
    journal, May 2014

    • Bertucci, Alessandro; Lülf, Henning; Septiadi, Dedy
    • Advanced Healthcare Materials, Vol. 3, Issue 11
    • DOI: 10.1002/adhm.201400116

    Effect of Shape, Size, and Aspect Ratio on Nanoparticle Penetration and Distribution inside Solid Tissues Using 3D Spheroid Models
    journal, September 2015

    • Agarwal, Rachit; Jurney, Patrick; Raythatha, Mansi
    • Advanced Healthcare Materials, Vol. 4, Issue 15
    • DOI: 10.1002/adhm.201500441

    Fluorescent Nanorods and Nanospheres for Real-Time In Vivo Probing of Nanoparticle Shape-Dependent Tumor Penetration
    journal, October 2011

    • Chauhan, Vikash P.; Popović, Zoran; Chen, Ou
    • Angewandte Chemie International Edition, Vol. 50, Issue 48
    • DOI: 10.1002/anie.201104449

    Solid-Phase synthesis of peptide nucleic acids
    journal, May 1995

    • Christensen, Leif; Fitzpatrick, Richard; Gildea, Brian
    • Journal of Peptide Science, Vol. 1, Issue 3
    • DOI: 10.1002/psc.310010304

    Effect of Surface Properties on Nanoparticle–Cell Interactions
    journal, January 2010


    Combined Delivery of Temozolomide and Anti-miR221 PNA Using Mesoporous Silica Nanoparticles Induces Apoptosis in Resistant Glioma Cells
    journal, September 2015

    • Bertucci, Alessandro; Prasetyanto, Eko Adi; Septiadi, Dedy
    • Small, Vol. 11, Issue 42
    • DOI: 10.1002/smll.201500540

    Applications of PNA-laden nanoparticles for hematological disorders
    journal, November 2018

    • Malik, Shipra; Oyaghire, Stanley; Bahal, Raman
    • Cellular and Molecular Life Sciences, Vol. 76, Issue 6
    • DOI: 10.1007/s00018-018-2979-5

    The influence of size, shape and vessel geometry on nanoparticle distribution
    journal, July 2012


    Stability of peptide nucleic acids in human serum and cellular extracts
    journal, September 1994

    • Demidov, Vadim V.; Potaman, Vladimir N.; Frank-Kamenetskil, M. D.
    • Biochemical Pharmacology, Vol. 48, Issue 6
    • DOI: 10.1016/0006-2952(94)90171-6

    Lipid–protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes
    journal, March 2012

    • Lyukmanova, E. N.; Shenkarev, Z. O.; Khabibullina, N. F.
    • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1818, Issue 3
    • DOI: 10.1016/j.bbamem.2011.10.020

    The effects of temperature, salinity, concentration and PEGylated lipid on the spontaneous nanostructures of bicellar mixtures
    journal, July 2014

    • Liu, Ying; Li, Ming; Yang, Yongkun
    • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1838, Issue 7
    • DOI: 10.1016/j.bbamem.2014.02.004

    Differential surface properties of commercial crystalline telmisartan samples
    journal, May 2013

    • Laad, Preksha; Shete, Ganesh; Modi, Sameer R.
    • European Journal of Pharmaceutical Sciences, Vol. 49, Issue 2
    • DOI: 10.1016/j.ejps.2013.02.017

    The effect of shape on the margination dynamics of non-neutrally buoyant particles in two-dimensional shear flows
    journal, July 2008


    Endocytosis of nanomedicines
    journal, August 2010


    Nanotechnology for delivery of peptide nucleic acids (PNAs)
    journal, October 2016


    Anti-tumor Activity of miniPEG-γ-Modified PNAs to Inhibit MicroRNA-210 for Cancer Therapy
    journal, December 2017


    Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes
    journal, September 2005


    Effects of Membrane Defects and Polymer Hydrophobicity on Networking Kinetics of Vesicles
    journal, May 2017


    Lipid Nanodisc-Templated Self-Assembly of Gold Nanoparticles into Strings and Rings
    journal, March 2017


    Synthesis of Conformationally Preorganized and Cell-Permeable Guanidine-Based γ-Peptide Nucleic Acids (γGPNAs)
    journal, February 2009

    • Sahu, Bichismita; Chenna, Venugopal; Lathrop, Kira L.
    • The Journal of Organic Chemistry, Vol. 74, Issue 4
    • DOI: 10.1021/jo802211n

    Fusion of Ligand-Coated Nanoparticles with Lipid Bilayers: Effect of Ligand Flexibility
    journal, May 2014

    • Van Lehn, Reid C.; Alexander-Katz, Alfredo
    • The Journal of Physical Chemistry A, Vol. 118, Issue 31
    • DOI: 10.1021/jp411662c

    Smart Photothermal-Triggered Bilayer Phase Transition in AuNPs–Liposomes to Release Drug
    journal, January 2013

    • An, Xueqin; Zhan, Fan; Zhu, Yinyan
    • Langmuir, Vol. 29, Issue 4
    • DOI: 10.1021/la304692h

    The Effects of Particle Size and Molecular Targeting on the Intratumoral and Subcellular Distribution of Polymeric Nanoparticles
    journal, May 2010

    • Lee, Helen; Fonge, Humphrey; Hoang, Bryan
    • Molecular Pharmaceutics, Vol. 7, Issue 4
    • DOI: 10.1021/mp100038h

    Hydrophobic Gold Nanoparticle Self-Assembly with Phosphatidylcholine Lipid: Membrane-Loaded and Janus Vesicles
    journal, September 2010

    • Rasch, Michael R.; Rossinyol, Emma; Hueso, Jose L.
    • Nano Letters, Vol. 10, Issue 9
    • DOI: 10.1021/nl102387n

    Structural and Thermal Analysis of Lipid Vesicles Encapsulating Hydrophobic Gold Nanoparticles
    journal, May 2012

    • Von White, Gregory; Chen, Yanjing; Roder-Hanna, Julia
    • ACS Nano, Vol. 6, Issue 6
    • DOI: 10.1021/nn2042016

    Self-Assembled Porphyrin Nanodiscs with Structure-Dependent Activation for Phototherapy and Photodiagnostic Applications
    journal, March 2013

    • Ng, Kenneth K.; Lovell, Jonathan F.; Vedadi, Ali
    • ACS Nano, Vol. 7, Issue 4
    • DOI: 10.1021/nn400418y

    PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules
    journal, October 1993

    • Egholm, Michael; Buchardt, Ole; Christensen, Leif
    • Nature, Vol. 365, Issue 6446
    • DOI: 10.1038/365566a0

    Control of Endothelial Targeting and Intracellular Delivery of Therapeutic Enzymes by Modulating the Size and Shape of ICAM-1-targeted Carriers
    journal, August 2008

    • Muro, Silvia; Garnacho, Carmen; Champion, Julie A.
    • Molecular Therapy, Vol. 16, Issue 8
    • DOI: 10.1038/mt.2008.127

    MicroRNA silencing for cancer therapy targeted to the tumour microenvironment
    journal, November 2014

    • Cheng, Christopher J.; Bahal, Raman; Babar, Imran A.
    • Nature, Vol. 518, Issue 7537
    • DOI: 10.1038/nature13905

    Principles of nanoparticle design for overcoming biological barriers to drug delivery
    journal, September 2015

    • Blanco, Elvin; Shen, Haifa; Ferrari, Mauro
    • Nature Biotechnology, Vol. 33, Issue 9
    • DOI: 10.1038/nbt.3330

    In vivo correction of anaemia in β-thalassemic mice by γPNA-mediated gene editing with nanoparticle delivery
    journal, October 2016

    • Bahal, Raman; Ali McNeer, Nicole; Quijano, Elias
    • Nature Communications, Vol. 7, Issue 1
    • DOI: 10.1038/ncomms13304

    Lipid tail protrusions mediate the insertion of nanoparticles into model cell membranes
    journal, July 2014

    • Van Lehn, Reid C.; Ricci, Maria; Silva, Paulo H. J.
    • Nature Communications, Vol. 5, Issue 1
    • DOI: 10.1038/ncomms5482

    In utero nanoparticle delivery for site-specific genome editing
    journal, June 2018


    Guiding the location of nanoparticles into vesicular structures: a morphological study
    journal, January 2007

    • Binder, Wolfgang H.; Sachsenhofer, Robert; Farnik, Dominique
    • Physical Chemistry Chemical Physics, Vol. 9, Issue 48
    • DOI: 10.1039/b711470m

    Nanoparticle-based therapy in an in vivo microRNA-155 (miR-155)-dependent mouse model of lymphoma
    journal, June 2012

    • Babar, I. A.; Cheng, C. J.; Booth, C. J.
    • Proceedings of the National Academy of Sciences, Vol. 109, Issue 26
    • DOI: 10.1073/pnas.1201516109

    Mammalian cells preferentially internalize hydrogel nanodiscs over nanorods and use shape-specific uptake mechanisms
    journal, October 2013

    • Agarwal, R.; Singh, V.; Jurney, P.
    • Proceedings of the National Academy of Sciences, Vol. 110, Issue 43
    • DOI: 10.1073/pnas.1305000110

    A Truncated HIV-1 Tat Protein Basic Domain Rapidly Translocates through the Plasma Membrane and Accumulates in the Cell Nucleus
    journal, June 1997

    • Vivès, Eric; Brodin, Priscille; Lebleu, Bernard
    • Journal of Biological Chemistry, Vol. 272, Issue 25
    • DOI: 10.1074/jbc.272.25.16010

    Small unilamellar vesicles: a platform technology for molecular imaging of brain tumors
    journal, March 2011


    Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide
    journal, December 1991


    The Effect of Nanoparticle Size, Shape, and Surface Chemistry on Biological Systems
    journal, August 2012


    The Morphology of Self-Assembled Lipid-Based Nanoparticles Affects Their Uptake by Cancer Cells
    journal, October 2016

    • Aresh, Wafa; Liu, Ying; Sine, Jessica
    • Journal of Biomedical Nanotechnology, Vol. 12, Issue 10
    • DOI: 10.1166/jbn.2016.2292

    Advances in Nanoparticle-based Delivery of Next Generation Peptide Nucleic Acids
    journal, March 2019



    journal, October 2013


    Nanoparticle for delivery of antisense γPNA oligomers targeting CCR5
    journal, April 2013

    • Bahal, Raman; McNeer, Nicole Ali; Ly, Danith H.
    • Artificial DNA: PNA & XNA, Vol. 4, Issue 2
    • DOI: 10.4161/adna.25628