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Title: Cytosolic lipid droplets as engineered organelles for production and accumulation of terpenoid biomaterials in leaves

Abstract

Cytosolic lipid droplets are endoplasmic reticulum-derived organelles typically found in seeds as reservoirs for physiological energy and carbon to fuel germination. In this work, we report synthetic biology approaches to co-produce high-value sesqui- or diterpenoids together with lipid droplets in plant leaves. The formation of cytosolic lipid droplets is enhanced in the transient Nicotiana benthamiana system through ectopic production of WRINKLED1, a key regulator of plastid fatty acid biosynthesis, and a microalgal lipid droplet surface protein. Engineering of the pathways providing the universal C5-building blocks for terpenoids and installation of terpenoid biosynthetic pathways through direction of the enzymes to native and non-native compartments boost the production of target terpenoids. We demonstrate that anchoring of distinct biosynthetic steps onto the surface of lipid droplets leads to efficient production of terpenoid scaffolds and functionalized terpenoids. The co-produced lipid droplets “trap” the terpenoids in the cells.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [2];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]
  1. Michigan State Univ., East Lansing, MI (United States); USDOE Great Lakes Bioenergy Research Center, East Lansing, MI (United States)
  2. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
OSTI Identifier:
1556068
Grant/Contract Number:  
SC0018409; FC02-07ER64494; FG02-91ER20021
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Sadre, Radin, Kuo, Peiyen, Chen, Jiaxing, Yang, Yang, Banerjee, Aparajita, Benning, Christoph, and Hamberger, Bjoern. Cytosolic lipid droplets as engineered organelles for production and accumulation of terpenoid biomaterials in leaves. United States: N. p., 2019. Web. doi:10.1038/s41467-019-08515-4.
Sadre, Radin, Kuo, Peiyen, Chen, Jiaxing, Yang, Yang, Banerjee, Aparajita, Benning, Christoph, & Hamberger, Bjoern. Cytosolic lipid droplets as engineered organelles for production and accumulation of terpenoid biomaterials in leaves. United States. doi:10.1038/s41467-019-08515-4.
Sadre, Radin, Kuo, Peiyen, Chen, Jiaxing, Yang, Yang, Banerjee, Aparajita, Benning, Christoph, and Hamberger, Bjoern. Wed . "Cytosolic lipid droplets as engineered organelles for production and accumulation of terpenoid biomaterials in leaves". United States. doi:10.1038/s41467-019-08515-4. https://www.osti.gov/servlets/purl/1556068.
@article{osti_1556068,
title = {Cytosolic lipid droplets as engineered organelles for production and accumulation of terpenoid biomaterials in leaves},
author = {Sadre, Radin and Kuo, Peiyen and Chen, Jiaxing and Yang, Yang and Banerjee, Aparajita and Benning, Christoph and Hamberger, Bjoern},
abstractNote = {Cytosolic lipid droplets are endoplasmic reticulum-derived organelles typically found in seeds as reservoirs for physiological energy and carbon to fuel germination. In this work, we report synthetic biology approaches to co-produce high-value sesqui- or diterpenoids together with lipid droplets in plant leaves. The formation of cytosolic lipid droplets is enhanced in the transient Nicotiana benthamiana system through ectopic production of WRINKLED1, a key regulator of plastid fatty acid biosynthesis, and a microalgal lipid droplet surface protein. Engineering of the pathways providing the universal C5-building blocks for terpenoids and installation of terpenoid biosynthetic pathways through direction of the enzymes to native and non-native compartments boost the production of target terpenoids. We demonstrate that anchoring of distinct biosynthetic steps onto the surface of lipid droplets leads to efficient production of terpenoid scaffolds and functionalized terpenoids. The co-produced lipid droplets “trap” the terpenoids in the cells.},
doi = {10.1038/s41467-019-08515-4},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {2019},
month = {2}
}

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