Pore-scale hydrodynamics influence the spatial evolution of bacterial biofilms in a microfluidic porous network

Aufrecht, Jayde A.; Fowlkes, Jason Davidson; Webb, Amber N.; Morrell-Falvey, Jennifer L.; Doktycz, Mitchel John; Retterer, Scott T.

doi:10.1371/journal.pone.0218316

Title: Pore-scale hydrodynamics influence the spatial evolution of bacterial biofilms in a microfluidic porous network

Journal Article · 26 June 2019 · PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0218316 · OSTI ID:1550761

Aufrecht, Jayde A. ^[1];

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Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)

Bacteria occupy heterogeneous environments, attaching and growing within pores in materials, living hosts, and matrices like soil. Systems that permit high-resolution visualization of dynamic bacterial processes within the physical confines of a realistic and tractable porous media environment are rare. In this paper, we use microfluidics to replicate the grain shape and packing density of natural sands in a 2D platform to study the flow-induced spatial evolution of bacterial biofilms underground. We discover that initial bacterial dispersal and grain attachment is influenced by bacterial transport across pore space velocity gradients, a phenomenon otherwise known as rheotaxis. We find that gravity-driven flow conditions activate different bacterial cell-clustering phenotypes depending on the strain’s ability to product extracellular polymeric substances (EPS). A wildtype, biofilm-producing bacteria formed compact, multicellular patches while an EPS-defective mutant displayed a linked-cell phenotype in the presence of flow. Finally, these phenotypes subsequently influenced the overall spatial distribution of cells across the porous media network as colonies grew and altered the fluid dynamics of their microenvironment.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1550761

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 6 Vol. 14; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

References (82)

A web of streamers: biofilm formation in a porous microfluidic device Valiei, Amin; Kumar, Aloke; Mukherjee, Partha P. Lab on a Chip, Vol. 12, Issue 24 https://doi.org/10.1039/c2lc40815e	journal	January 2012
Bacterial rheotaxis Proceedings of the National Academy of Sciences, Vol. 109, Issue 13 https://doi.org/10.1073/pnas.1120955109	journal	March 2012
Microscale confinement features can affect biofilm formation Kumar, Aloke; Karig, David; Acharya, Rajesh Microfluidics and Nanofluidics, Vol. 14, Issue 5 https://doi.org/10.1007/s10404-012-1120-6	journal	December 2012
Biofilm streamers cause catastrophic disruption of flow with consequences for environmental and medical systems. Drescher, Knut; Shen, Yi; Bassler, Bonnie L. Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.34324	text	January 2013
Pathogen transport in groundwater systems: contrasts with traditional solute transport Hunt, Randall J.; Johnson, William P. Hydrogeology Journal, Vol. 25, Issue 4 https://doi.org/10.1007/s10040-016-1502-z	journal	December 2016
Prokaryotes: The unseen majority Whitman, W. B.; Coleman, D. C.; Wiebe, W. J. Proceedings of the National Academy of Sciences, Vol. 95, Issue 12 https://doi.org/10.1073/pnas.95.12.6578	journal	June 1998
The curved shape of Caulobacter crescentus enhances surface colonization in flow Persat, Alexandre; Stone, Howard A.; Gitai, Zemer Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4824	journal	May 2014
Twenty-One Genome Sequences from Pseudomonas Species and 19 Genome Sequences from Diverse Bacteria Isolated from the Rhizosphere and Endosphere of Populus deltoides Brown, S. D.; Utturkar, S. M.; Klingeman, D. M. Journal of Bacteriology, Vol. 194, Issue 21 https://doi.org/10.1128/JB.01243-12	journal	October 2012
Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins P., Zimmerman, Seth; Gurkan, Guntas,; Tishan, Williams, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/34fj-dp19	text	January 2015
Transport and straining of E. coli O157:H7 in saturated porous media : TRANSPORT AND STRAINING OF Bradford, Scott A.; Simunek, Jirka; Walker, Sharon L. Water Resources Research, Vol. 42, Issue 12 https://doi.org/10.1029/2005WR004805	journal	November 2006
Biosynthesis of the repeating units of the exopolysaccharides amylovoran from Erwinia amylovora and stewartan from Pantoea stewartii Langlotz, Christine; Schollmeyer, Martin; Coplin, David L. Physiological and Molecular Plant Pathology, Vol. 75, Issue 4 https://doi.org/10.1016/j.pmpp.2011.04.001	journal	April 2011
Enhanced Transverse Migration of Bacteria by Chemotaxis in a Porous T-Sensor Long, Tao; Ford, Roseanne M. Environmental Science & Technology, Vol. 43, Issue 5 https://doi.org/10.1021/es802558j	journal	March 2009
Spatial distribution of bacterial communities and their relationships with the micro-architecture of soil Nunan, Naoise; Wu, Kejian; Young, Iain M. FEMS Microbiology Ecology, Vol. 44, Issue 2 https://doi.org/10.1016/s0168-6496(03)00027-8	journal	May 2003
Processes in Microbial Transport in the Natural Subsurface Ginn, Timothy R.; Wood, Brian D.; Nelson, Kirk E. ChemInform, Vol. 34, Issue 23 https://doi.org/10.1002/chin.200323283	journal	June 2003
Active Starvation Responses Mediate Antibiotic Tolerance in Biofilms and Nutrient-Limited Bacteria Nguyen, D.; Joshi-Datar, A.; Lepine, F. Science, Vol. 334, Issue 6058, p. 982-986 https://doi.org/10.1126/science.1211037	journal	November 2011
Single- and two-phase flow in microfluidic porous media analogs based on Voronoi tessellation Wu, Mengjie; Xiao, Feng; Johnson-Paben, Rebecca M. Lab Chip, Vol. 12, Issue 2 https://doi.org/10.1039/c1lc20838a	journal	January 2012
Transverse Chemotactic Migration of Bacteria from High to Low Permeability Regions in a Dual Permeability Microfluidic Device Singh, Rajveer; Olson, Mira Stone Environmental Science & Technology, Vol. 46, Issue 6 https://doi.org/10.1021/es203614y	journal	March 2012
Defined spatial structure stabilizes a synthetic multispecies bacterial community Kim, H. J.; Boedicker, J. Q.; Choi, J. W. Proceedings of the National Academy of Sciences, Vol. 105, Issue 47 https://doi.org/10.1073/pnas.0807935105	journal	November 2008
Microfluidics Expanding the Frontiers of Microbial Ecology Rusconi, Roberto; Garren, Melissa; Stocker, Roman Annual Review of Biophysics, Vol. 43, Issue 1 https://doi.org/10.1146/annurev-biophys-051013-022916	journal	May 2014
Influence of biofilm accumulation on porous media hydrodynamics Cunningham, Alfred B.; Characklis, William G.; Abedeen, Feisal Environmental Science & Technology, Vol. 25, Issue 7 https://doi.org/10.1021/es00019a013	journal	July 1991
Effects of Pore-Scale Heterogeneity and Transverse Mixing on Bacterial Growth in Porous Media Zhang, Changyong; Kang, Qinjun; Wang, Xing Environmental Science & Technology, Vol. 44, Issue 8 https://doi.org/10.1021/es903396h	journal	April 2010
Microfluidic Confinement of Single Cells of Bacteria in Small Volumes Initiates High-Density Behavior of Quorum Sensing and Growth and Reveals Its Variability Boedicker, James Q.; Vincent, Meghan E.; Ismagilov, Rustem F. Angewandte Chemie International Edition, Vol. 48, Issue 32 https://doi.org/10.1002/anie.200901550	journal	July 2009
Reservoir-on-a-Chip (ROC): A new paradigm in reservoir engineering Kumar Gunda, Naga Siva; Bera, Bijoyendra; Karadimitriou, Nikolaos K. Lab on a Chip, Vol. 11, Issue 22 https://doi.org/10.1039/c1lc20556k	journal	January 2011
Microfluidics Expanding the Frontiers of Microbial Ecology Rusconi, Roberto; Garren, Melissa; Stocker, Roman Annual Review of Biophysics, Vol. 43, Issue 1 https://doi.org/10.1146/annurev-biophys-051013-022916	journal	May 2014
Role of chemotaxis in the transport of bacteria through saturated porous media Ford, Roseanne M.; Harvey, Ronald W. Advances in Water Resources, Vol. 30, Issue 6-7 https://doi.org/10.1016/j.advwatres.2006.05.019	journal	June 2007
Bacterial floc mediated rapid streamer formation in creeping flows Hassanpourfard, Mahtab; Nikakhtari, Zahra; Ghosh, Ranajay Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep13070	journal	August 2015
Bacterial transport suppressed by fluid shear Rusconi, Roberto; Guasto, Jeffrey S.; Stocker, Roman Nature Physics, Vol. 10, Issue 3 https://doi.org/10.1038/nphys2883	journal	February 2014
Multiscale responses of microbial life to spatial distance and environmental heterogeneity in a patchy ecosystem Tiedje, J. M.; Ramette, Alban Nicolas National Academy of Sciences NAS https://doi.org/10.7892/boris.91416	text	January 2007
Biofilm streamers cause catastrophic disruption of flow with consequences for environmental and medical systems Drescher, Knut; Shen, Yi; Bassler, Bonnie L. National Academy of Sciences https://doi.org/10.5451/unibas-ep83559	text	January 2013
Negated bio-events: analysis and identification Nawaz, Raheel; Thompson, Paul; Ananiadou, Sophia BMC Bioinformatics, Vol. 14, Issue 1 https://doi.org/10.1186/1471-2105-14-14	journal	January 2013
Spatial distribution of bacterial communities and their relationships with the micro-architecture of soil Nunan, Naoise; Wu, Kejian; Young, Iain M. FEMS Microbiology Ecology, Vol. 44, Issue 2 https://doi.org/10.1016/S0168-6496(03)00027-8	journal	May 2003
Laminar flow around corners triggers the formation of biofilm streamers Rusconi, Roberto; Lecuyer, Sigolene; Guglielmini, Laura Journal of The Royal Society Interface, Vol. 7, Issue 50 https://doi.org/10.1098/rsif.2010.0096	journal	February 2010
Analysis of Bacterial Random Motility in a Porous Medium Using Magnetic Resonance Imaging and Immunomagnetic Labeling Sherwood, Juli L.; Sung, James C.; Ford, Roseanne M. Environmental Science & Technology, Vol. 37, Issue 4 https://doi.org/10.1021/es011210u	journal	February 2003
Visualization, modelling and prediction in soil microbiology O'Donnell, Anthony G.; Young, Iain M.; Rushton, Steven P. Nature Reviews Microbiology, Vol. 5, Issue 9 https://doi.org/10.1038/nrmicro1714	journal	September 2007
Live from under the lens: exploring microbial motility with dynamic imaging and microfluidics Son, Kwangmin; Brumley, Douglas R.; Stocker, Roman Nature Reviews Microbiology, Vol. 13, Issue 12 https://doi.org/10.1038/nrmicro3567	journal	November 2015
Live from under the lens: exploring microbial motility with dynamic imaging and microfluidics Son, Kwangmin; Brumley, Douglas R.; Stocker, Roman Nature Reviews Microbiology, Vol. 13, Issue 12 https://doi.org/10.1038/nrmicro3567	journal	November 2015
Microfluidic Confinement of Single Cells of Bacteria in Small Volumes Initiates High-Density Behavior of Quorum Sensing and Growth and Reveals Its Variability Boedicker, James Q.; Vincent, Meghan E.; Ismagilov, Rustem F. Angewandte Chemie International Edition, Vol. 48, Issue 32 https://doi.org/10.1002/anie.200901550	journal	July 2009
Cultivation of unculturable soil bacteria Pham, Van H. T.; Kim, Jaisoo Trends in Biotechnology, Vol. 30, Issue 9 https://doi.org/10.1016/j.tibtech.2012.05.007	journal	September 2012
Revisiting the STEC Testing Approach: Using espK and espV to Make Enterohemorrhagic Escherichia coli (EHEC) Detection More Reliable in Beef Delannoy, Sabine; Chaves, Byron D.; Ison, Sarah A. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.00001	journal	January 2016
The rhizosphere: complex by design Jones, D. L.; Hinsinger, P. Plant and Soil, Vol. 312, Issue 1-2 https://doi.org/10.1007/s11104-008-9774-2	journal	September 2008
Interplay of physical mechanisms and biofilm processes: review of microfluidic methods Karimi, A.; Karig, D.; Kumar, A. Lab on a Chip, Vol. 15, Issue 1 https://doi.org/10.1039/c4lc01095g	journal	January 2015
Multiscale responses of microbial life to spatial distance and environmental heterogeneity in a patchy ecosystem Ramette, A.; Tiedje, J. M. Proceedings of the National Academy of Sciences, Vol. 104, Issue 8 https://doi.org/10.1073/pnas.0610671104	journal	February 2007
Cultivation of unculturable soil bacteria Pham, Van H. T.; Kim, Jaisoo Trends in Biotechnology, Vol. 30, Issue 9 https://doi.org/10.1016/j.tibtech.2012.05.007	journal	September 2012
Processes in microbial transport in the natural subsurface Ginn, Timothy R.; Wood, Brian D.; Nelson, Kirk E. Advances in Water Resources, Vol. 25, Issue 8-12 https://doi.org/10.1016/S0309-1708(02)00046-5	journal	August 2002
Enhanced Transverse Migration of Bacteria by Chemotaxis in a Porous T-Sensor Long, Tao; Ford, Roseanne M. Environmental Science & Technology, Vol. 43, Issue 5 https://doi.org/10.1021/es802558j	journal	March 2009
Laminar flow around corners triggers the formation of biofilm streamers Rusconi, Roberto; Lecuyer, Sigolene; Guglielmini, Laura Journal of The Royal Society Interface, Vol. 7, Issue 50 https://doi.org/10.1098/rsif.2010.0096	journal	February 2010
Transverse Chemotactic Migration of Bacteria from High to Low Permeability Regions in a Dual Permeability Microfluidic Device Singh, Rajveer; Olson, Mira Stone Environmental Science & Technology, Vol. 46, Issue 6 https://doi.org/10.1021/es203614y	journal	March 2012
A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization Bible, Amber N.; Fletcher, Sarah J.; Pelletier, Dale A. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.00491	journal	April 2016
Microbes in flow Rusconi, Roberto; Stocker, Roman Current Opinion in Microbiology, Vol. 25 https://doi.org/10.1016/j.mib.2015.03.003	journal	June 2015
Use of Ichip for High-Throughput In Situ Cultivation of "Uncultivable" Microbial Species Nichols, D.; Cahoon, N.; Trakhtenberg, E. M. Applied and Environmental Microbiology, Vol. 76, Issue 8 https://doi.org/10.1128/aem.01754-09	journal	February 2010
Rhizosphere Soil Aggregation and Plant Growth Promotion of Sunflowers by an Exopolysaccharide-Producing Rhizobium sp. Strain Isolated from Sunflower Roots Alami, Y.; Achouak, W.; Marol, C. Applied and Environmental Microbiology, Vol. 66, Issue 8 https://doi.org/10.1128/AEM.66.8.3393-3398.2000	journal	August 2000
Biosynthesis of the repeating units of the exopolysaccharides amylovoran from Erwinia amylovora and stewartan from Pantoea stewartii Langlotz, Christine; Schollmeyer, Martin; Coplin, David L. Physiological and Molecular Plant Pathology, Vol. 75, Issue 4 https://doi.org/10.1016/j.pmpp.2011.04.001	journal	April 2011
Microfluidic Confinement of Single Cells of Bacteria in Small Volumes Initiates High-Density Behavior of Quorum Sensing and Growth and Reveals Its Variability Boedicker, James Q.; Vincent, Meghan E.; Ismagilov, Rustem F. Angewandte Chemie, Vol. 121, Issue 32 https://doi.org/10.1002/ange.200901550	journal	June 2009
Fourier–Voronoi-based generation of realistic samples for discrete modelling of granular materials Mollon, Guilhem; Zhao, Jidong Granular Matter, Vol. 14, Issue 5 https://doi.org/10.1007/s10035-012-0356-x	journal	June 2012
Challenges in modeling unstable two-phase flow experiments in porous micromodels Ferrari, Andrea; Jimenez-Martinez, Joaquin; Borgne, Tanguy Le Water Resources Research, Vol. 51, Issue 3 https://doi.org/10.1002/2014WR016384	journal	March 2015
Microscale confinement features can affect biofilm formation Kumar, Aloke; Karig, David; Acharya, Rajesh Microfluidics and Nanofluidics, Vol. 14, Issue 5 https://doi.org/10.1007/s10404-012-1120-6	journal	December 2012
Biofilm streamers cause catastrophic disruption of flow with consequences for environmental and medical systems Drescher, Knut; Shen, Yi; Bassler, Bonnie L. Proceedings of the National Academy of Sciences, Vol. 110, Issue 11 https://doi.org/10.1073/pnas.1300321110	journal	February 2013
Fourier–Voronoi-based generation of realistic samples for discrete modelling of granular materials Mollon, Guilhem; Zhao, Jidong Granular Matter, Vol. 14, Issue 5 https://doi.org/10.1007/s10035-012-0356-x	journal	June 2012
Defined spatial structure stabilizes a synthetic multispecies bacterial community Kim, H. J.; Boedicker, J. Q.; Choi, J. W. Proceedings of the National Academy of Sciences, Vol. 105, Issue 47 https://doi.org/10.1073/pnas.0807935105	journal	November 2008
Flexible Methods for Microfluidics Whitesides, George M.; Stroock, Abraham D. Physics Today, Vol. 54, Issue 6 https://doi.org/10.1063/1.1387591	journal	June 2001
First principles of terrestrial life: exemplars for potential extra-terrestrial biology von Hegner, Ian Theory in Biosciences, Vol. 141, Issue 3 https://doi.org/10.1007/s12064-022-00373-x	journal	July 2022
Pore geometry effects on intrapore viscous to inertial flows and on effective hydraulic parameters: Effect of Pore Geometry in Modifying the Flow Field Chaudhary, Kuldeep; Cardenas, M. Bayani; Deng, Wen Water Resources Research, Vol. 49, Issue 2 https://doi.org/10.1002/wrcr.20099	journal	February 2013
Synergistic effects of soil microstructure and bacterial EPS on drying rate in emulated soil micromodels Deng, Jinzi; Orner, Erika P.; Chau, Jessica Furrer Soil Biology and Biochemistry, Vol. 83 https://doi.org/10.1016/j.soilbio.2014.12.006	journal	April 2015
Pore geometry effects on intrapore viscous to inertial flows and on effective hydraulic parameters: Effect of Pore Geometry in Modifying the Flow Field Chaudhary, Kuldeep; Cardenas, M. Bayani; Deng, Wen Water Resources Research, Vol. 49, Issue 2 https://doi.org/10.1002/wrcr.20099	journal	February 2013
Influence of biofilm accumulation on porous media hydrodynamics Cunningham, Alfred B.; Characklis, William G.; Abedeen, Feisal Environmental Science & Technology, Vol. 25, Issue 7 https://doi.org/10.1021/es00019a013	journal	July 1991
Single- and two-phase flow in microfluidic porous media analogs based on Voronoi tessellation Wu, Mengjie; Xiao, Feng; Johnson-Paben, Rebecca M. Lab Chip, Vol. 12, Issue 2 https://doi.org/10.1039/C1LC20838A	journal	January 2012
Statistical method on nonrandom clustering with application to somatic mutations in cancer Ye, Jingjing; Pavlicek, Adam; Lunney, Elizabeth A. BMC Bioinformatics, Vol. 11, Issue 1 https://doi.org/10.1186/1471-2105-11-11	journal	January 2010
Bacterial rheotaxis Proceedings of the National Academy of Sciences, Vol. 109, Issue 13 https://doi.org/10.1073/pnas.1120955109	journal	March 2012
Fiji: an open-source platform for biological-image analysis Schindelin, Johannes; Arganda-Carreras, Ignacio; Frise, Erwin Nature Methods, Vol. 9, Issue 7 https://doi.org/10.1038/nmeth.2019	journal	June 2012
Microbes in flow Rusconi, Roberto; Stocker, Roman Current Opinion in Microbiology, Vol. 25 https://doi.org/10.1016/j.mib.2015.03.003	journal	June 2015
Role of chemotaxis in the transport of bacteria through saturated porous media Ford, Roseanne M.; Harvey, Ronald W. Advances in Water Resources, Vol. 30, Issue 6-7 https://doi.org/10.1016/j.advwatres.2006.05.019	journal	June 2007
Twenty-One Genome Sequences from Pseudomonas Species and 19 Genome Sequences from Diverse Bacteria Isolated from the Rhizosphere and Endosphere of Populus deltoides Brown, S. D.; Utturkar, S. M.; Klingeman, D. M. Journal of Bacteriology, Vol. 194, Issue 21 https://doi.org/10.1128/jb.01243-12	journal	October 2012
Bacterial mechanotransduction Persat, Alexandre Current Opinion in Microbiology, Vol. 36 https://doi.org/10.1016/j.mib.2016.12.002	journal	April 2017
Reservoir-on-a-Chip (ROC): A new paradigm in reservoir engineering Kumar Gunda, Naga Siva; Bera, Bijoyendra; Karadimitriou, Nikolaos K. Lab on a Chip, Vol. 11, Issue 22 https://doi.org/10.1039/c1lc20556k	journal	January 2011
Use of Ichip for High-Throughput In Situ Cultivation of "Uncultivable" Microbial Species Nichols, D.; Cahoon, N.; Trakhtenberg, E. M. Applied and Environmental Microbiology, Vol. 76, Issue 8 https://doi.org/10.1128/AEM.01754-09	journal	February 2010
Bacterial mechanotransduction Persat, Alexandre Current Opinion in Microbiology, Vol. 36 https://doi.org/10.1016/j.mib.2016.12.002	journal	April 2017
A web of streamers: biofilm formation in a porous microfluidic device Valiei, Amin; Kumar, Aloke; Mukherjee, Partha P. Lab on a Chip, Vol. 12, Issue 24 https://doi.org/10.1039/c2lc40815e	journal	January 2012
Multiscale responses of microbial life to spatial distance and environmental heterogeneity in a patchy ecosystem Ramette, A.; Tiedje, J. M. Proceedings of the National Academy of Sciences, Vol. 104, Issue 8 https://doi.org/10.1073/pnas.0610671104	journal	February 2007
Quantifying the Spatiotemporal Dynamics of Plant Root Colonization by Beneficial Bacteria in a Microfluidic Habitat Aufrecht, Jayde A.; Timm, Collin M.; Bible, Amber Advanced Biosystems, Vol. 2, Issue 6 https://doi.org/10.1002/adbi.201800048	journal	April 2018
Effects of Pore-Scale Heterogeneity and Transverse Mixing on Bacterial Growth in Porous Media Zhang, Changyong; Kang, Qinjun; Wang, Xing Environmental Science & Technology, Vol. 44, Issue 8 https://doi.org/10.1021/es903396h	journal	April 2010
Active Starvation Responses Mediate Antibiotic Tolerance in Biofilms and Nutrient-Limited Bacteria Nguyen, D.; Joshi-Datar, A.; Lepine, F. Science, Vol. 334, Issue 6058, p. 982-986 https://doi.org/10.1126/science.1211037	journal	November 2011
Physical constraints affecting bacterial habitats and activity in unsaturated porous media – a review Or, D.; Smets, B. F.; Wraith, J. M. Advances in Water Resources, Vol. 30, Issue 6-7 https://doi.org/10.1016/j.advwatres.2006.05.025	journal	June 2007

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