Genetically encoded sensors for monitoring the transport and concentration of nitrogen-containing and phosphorus-containing molecules in plants

Okumoto, Sakiko; Versaw, Wayne

doi:10.1016/j.pbi.2017.07.004

Title: Genetically encoded sensors for monitoring the transport and concentration of nitrogen-containing and phosphorus-containing molecules in plants

Full Record
Other Related Research

Authors:: Okumoto, Sakiko; Versaw, Wayne

Publication Date:: 2017-10-01

Sponsoring Org.:: USDOE

OSTI Identifier:: 1549704

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Current Opinion in Plant Biology

Additional Journal Information:: Journal Name: Current Opinion in Plant Biology Journal Volume: 39 Journal Issue: C; Journal ID: ISSN 1369-5266

Publisher:: Elsevier

Country of Publication:: United Kingdom

Language:: English

Citation Formats


                    Okumoto, Sakiko, and Versaw, Wayne. Genetically encoded sensors for monitoring the transport and concentration of nitrogen-containing and phosphorus-containing molecules in plants.  United Kingdom: N. p., 2017. 
Web.  doi:10.1016/j.pbi.2017.07.004.

Copy to clipboard


                    Okumoto, Sakiko, & Versaw, Wayne. Genetically encoded sensors for monitoring the transport and concentration of nitrogen-containing and phosphorus-containing molecules in plants.  United Kingdom.  https://doi.org/10.1016/j.pbi.2017.07.004

Copy to clipboard


                    Okumoto, Sakiko, and Versaw, Wayne. Sun .  
"Genetically encoded sensors for monitoring the transport and concentration of nitrogen-containing and phosphorus-containing molecules in plants".  United Kingdom.  https://doi.org/10.1016/j.pbi.2017.07.004.

Copy to clipboard


                    
@article{osti_1549704,

  title        = {Genetically encoded sensors for monitoring the transport and concentration of nitrogen-containing and phosphorus-containing molecules in plants},

  author       = {Okumoto, Sakiko and Versaw, Wayne},

  abstractNote = {},

  doi          = {10.1016/j.pbi.2017.07.004},

  journal      = {Current Opinion in Plant Biology},

  number       = C,

  volume       = 39,

  place        = {United Kingdom},

  year         = {2017},

  month        = {10}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (Publisher)

Publisher's Version of Record
https://doi.org/10.1016/j.pbi.2017.07.004

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 2 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE PAGES and OSTI.GOV collections:

Genetically encoded sensors for monitoring the transport and concentration of nitrogen-containing and phosphorus-containing molecules in plants

Journal Article Okumoto, Sakiko ; Versaw, Wayne - Current Opinion in Plant Biology

Not provided.
https://doi.org/10.1016/j.pbi.2017.07.004
Fluorescent protein Dendra2 as a ratiometric genetically encoded pH-sensor

Journal Article Pakhomov, Alexey A., E-mail: alpah@mail.ru ; Martynov, Vladimir I. ; Orsa, Alexander N. ; ... - Biochemical and Biophysical Research Communications

Fluorescent protein Dendra2 is a monomeric GFP-like protein that belongs to the group of Kaede-like photoconvertible fluorescent proteins with irreversible photoconversion from a green- to red-emitting state when exposed to violet-blue light. In an acidic environment, photoconverted Dendra2 turns green due to protonation of the phenolic group of the chromophore with pKa of about 7.5. Thus, photoconverted form of Dendra2 can be potentially used as a ratiometric pH-sensor in the physiological pH range. However, incomplete photoconversion makes ratiometric measurements irreproducible when using standard filter sets. Here, we describe the method to detect fluorescence of only photoconverted Dendra2 form, but notmore »« less
https://doi.org/10.1016/J.BBRC.2017.09.170
A genetically encoded, high-signal-to-noise maltose sensor

Journal Article Marvin, Jonathan S ; Schreiter, Eric R ; Echevarría, Ileabett M ; ... - Proteins

We describe the generation of a family of high-signal-to-noise single-wavelength genetically encoded indicators for maltose. This was achieved by insertion of circularly permuted fluorescent proteins into a bacterial periplasmic binding protein (PBP), Escherichia coli maltodextrin-binding protein, resulting in a four-color family of maltose indicators. The sensors were iteratively optimized to have sufficient brightness and maltose-dependent fluorescence increases for imaging, under both one- and two-photon illumination. We demonstrate that maltose affinity of the sensors can be tuned in a fashion largely independent of the fluorescent readout mechanism. Using literature mutations, the binding specificity could be altered to moderate sucrose preference, butmore »« less
https://doi.org/10.1002/prot.23118
Affinity Series of Genetically Encoded Förster Resonance Energy-Transfer Sensors for Sucrose

Journal Article Sadoine, Mayuri ; Reger, Mira ; Wong, Ka Man ; ... - ACS Sensors

Genetically encoded fluorescent sugar sensors are valuable tools for the discovery of transporters and for quantitative monitoring of sugar steady-state levels in intact tissues. Genetically encoded Förster resonance energy-transfer sensors for glucose have been designed and optimized extensively, and a full series of affinity mutants is available for in vivo studies. However, to date, only a single improved sucrose sensor FLIPsuc-90μΔ1 with K_m for sucrose of ~90 μM was available. This sucrose sensor was engineered on the basis of an Agrobacterium tumefaciens sugar-binding protein. Here, we took a two-step approach to first improve the dynamic range of the FLIPsuc sensormore »« less
https://doi.org/10.1021/acssensors.0c02495
Genetically encoded sensors for imaging proteins and their complexes

Patent Marriott, Gerard ; Hoepker, Alexander Chris ; Yan, Yuling

Isolated truncated and mutated sensor proteins derived from flavoproteins that are 12-20 KDa or less, genetically encoded for detection and imaging of protein complexes having long fluorescent lifetimes that can be 4.0 ns or greater.
Full Text Available

Similar Records