Interferon alpha inhibits replication of a live-attenuated porcine reproductive and respiratory syndrome virus vaccine preventing development of an adaptive immune response in swine

doi:10.1016/j.vetmic.2017.11.004

Title: Interferon alpha inhibits replication of a live-attenuated porcine reproductive and respiratory syndrome virus vaccine preventing development of an adaptive immune response in swine

Full Record
Other Related Research

Authors:: Brockmeier, Susan L.; Loving, Crystal L.; Eberle, Kirsten C.; Hau, Samantha J.; Buckley, Alexandra; Van Geelen, Albert; Montiel, Nestor A.; Nicholson, Tracy; Lager, Kelly M.

Publication Date:: 2017-12-01

Sponsoring Org.:: USDOE

OSTI Identifier:: 1549075

Grant/Contract Number:: AC05-06OR23100

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Veterinary Microbiology

Additional Journal Information:: Journal Name: Veterinary Microbiology Journal Volume: 212 Journal Issue: C; Journal ID: ISSN 0378-1135

Publisher:: Elsevier

Country of Publication:: Netherlands

Language:: English

Citation Formats


                    Brockmeier, Susan L., Loving, Crystal L., Eberle, Kirsten C., Hau, Samantha J., Buckley, Alexandra, Van Geelen, Albert, Montiel, Nestor A., Nicholson, Tracy, and Lager, Kelly M. Interferon alpha inhibits replication of a live-attenuated porcine reproductive and respiratory syndrome virus vaccine preventing development of an adaptive immune response in swine.  Netherlands: N. p., 2017. 
        Web.  doi:10.1016/j.vetmic.2017.11.004.

Copy to clipboard


                    Brockmeier, Susan L., Loving, Crystal L., Eberle, Kirsten C., Hau, Samantha J., Buckley, Alexandra, Van Geelen, Albert, Montiel, Nestor A., Nicholson, Tracy, & Lager, Kelly M. Interferon alpha inhibits replication of a live-attenuated porcine reproductive and respiratory syndrome virus vaccine preventing development of an adaptive immune response in swine.  Netherlands.  doi:10.1016/j.vetmic.2017.11.004.

Copy to clipboard


                    Brockmeier, Susan L., Loving, Crystal L., Eberle, Kirsten C., Hau, Samantha J., Buckley, Alexandra, Van Geelen, Albert, Montiel, Nestor A., Nicholson, Tracy, and Lager, Kelly M. Fri .  
        "Interferon alpha inhibits replication of a live-attenuated porcine reproductive and respiratory syndrome virus vaccine preventing development of an adaptive immune response in swine".  Netherlands.  doi:10.1016/j.vetmic.2017.11.004.

Copy to clipboard


                    
@article{osti_1549075,

  title        = {Interferon alpha inhibits replication of a live-attenuated porcine reproductive and respiratory syndrome virus vaccine preventing development of an adaptive immune response in swine},

  author       = {Brockmeier, Susan L. and Loving, Crystal L. and Eberle, Kirsten C. and Hau, Samantha J. and Buckley, Alexandra and Van Geelen, Albert and Montiel, Nestor A. and Nicholson, Tracy and Lager, Kelly M.},

  abstractNote = {},

  doi          = {10.1016/j.vetmic.2017.11.004},

  journal      = {Veterinary Microbiology},

  number       = C,

  volume       = 212,

  place        = {Netherlands},

  year         = {2017},

  month        = {12}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (Publisher)

Publisher's Version of Record
DOI: 10.1016/j.vetmic.2017.11.004

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 2 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE PAGES and OSTI.GOV collections:

Nonstructural protein 1{alpha} subunit-based inhibition of NF-{kappa}B activation and suppression of interferon-{beta} production by porcine reproductive and respiratory syndrome virus

Journal Article Cheng, Song ; Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61802 ; Krell, Peter ; ... - Virology

Induction of type I interferon (IFN-{alpha}/{beta}) is an early antiviral response of the host, and porcine reproductive and respiratory syndrome virus (PRRSV) has been reported to downregulate the IFN response during infection in cells and pigs. We report that the PRRSV nonstructural protein 1{alpha} (Nsp1{alpha}) subunit of Nsp1 is a nuclear-cytoplasmic protein distributed to the nucleus and contains a strong suppressive activity for IFN-{beta} production that is mediated through the retinoic acid-inducible gene I (RIG-I) signaling pathway. Nsp1{alpha} suppressed the activation of nuclear factor (NF)-{kappa}B when stimulated with dsRNA or tumor necrosis factor (TNF)-{alpha}, and NF-{kappa}B suppression was RIG-I-dependent. Themore »« less
DOI: 10.1016/j.virol.2010.08.025
Progress toward an enhanced vaccine: Eight marked attenuated viruses to porcine reproductive and respiratory disease virus

Journal Article Spear, Allyn ; Wang, Feng-Xue ; Kappes, Matthew A. ; ... - Virology
DOI: 10.1016/j.virol.2017.12.029
Mutations within the nuclear localization signal of the porcine reproductive and respiratory syndrome virus nucleocapsid protein attenuate virus replication

Journal Article Lee, Changhee ; Hodgins, Douglas ; Calvert, Jay G ; ... - Virology

Porcine reproductive and respiratory syndrome virus (PRRSV) is an RNA virus replicating in the cytoplasm, but the nucleocapsid (N) protein is specifically localized to the nucleus and nucleolus in virus-infected cells. A 'pat7' motif of 41-PGKK(N/S)KK has previously been identified in the N protein as the functional nuclear localization signal (NLS); however, the biological consequences of N protein nuclear localization are unknown. In the present study, the role of N protein nuclear localization during infection was investigated in pigs using an NLS-null mutant virus. When two lysines at 43 and 44 at the NLS locus were substituted to glycines, themore »« less
DOI: 10.1016/J.VIROL.2005.1
Glycoprotein 5 of porcine reproductive and respiratory syndrome virus strain SD16 inhibits viral replication and causes G2/M cell cycle arrest, but does not induce cellular apoptosis in Marc-145 cells

Journal Article Mu, Yang ; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People's Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100 ; Li, Liangliang ; ... - Virology

Cell apoptosis is common after infection with porcine reproductive and respiratory syndrome virus (PRRSV). PRRSV GP5 has been reported to induce cell apoptosis. To further understand the role of GP5 in PRRSV induced cell apoptosis, we established Marc-145 cell lines stably expressing full-length GP5, GP5{sup Δ84-96} (aa 84-96 deletion), and GP5{sup Δ97-119} (aa 97-119 deletion). Cell proliferation, cell cycle progression, cell apoptosis and virus replication in these cell lines were evaluated. Neither truncated nor full-length GP5 induced cell apoptosis in Marc-145 cells. However, GP5{sup Δ97-119}, but not full-length or GP5{sup Δ84-96}, induced a cell cycle arrest at the G2/M phasemore »« less
DOI: 10.1016/J.VIROL.2015.05.019
Nonstructural proteins nsp2TF and nsp2N of porcine reproductive and respiratory syndrome virus (PRRSV) play important roles in suppressing host innate immune responses

Journal Article Li, Y. ; Shang, P. ; Shyu, D. ; ... - Virology

Recently, we identified a unique -2/-1 ribosomal frameshift mechanism in PRRSV, which yields two truncated forms of nonstructural protein (nsp) 2 variants, nsp2TF and nsp2N. Here, in vitro expression of individual PRRSV nsp2TF and nsp2N demonstrated their ability to suppress cellular innate immune responses in transfected cells. Two recombinant viruses were further analyzed, in which either nsp2TF was C-terminally truncated (vKO1) or expression of both nsp2TF and nsp2N was knocked out (vKO2). Host cellular mRNA profiling showed that a panel of cellular immune genes, in particular those involved in innate immunity, was upregulated in cells infected with vKO1 and vKO2.more »« less
Cited by 7
DOI: 10.1016/j.virol.2017.12.017

Full Text Available

Similar Records