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Title: Steps towards ‘drop-in’ biofuels: focusing on metabolic pathways

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Grant/Contract Number:  
MCB1453147; D13AP00038; DESC0018324
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Current Opinion in Biotechnology
Additional Journal Information:
Journal Name: Current Opinion in Biotechnology Journal Volume: 53 Journal Issue: C; Journal ID: ISSN 0958-1669
Country of Publication:
United Kingdom

Citation Formats

Jiang, Wen, Gu, Pengfei, and Zhang, Fuzhong. Steps towards ‘drop-in’ biofuels: focusing on metabolic pathways. United Kingdom: N. p., 2018. Web. doi:10.1016/j.copbio.2017.10.010.
Jiang, Wen, Gu, Pengfei, & Zhang, Fuzhong. Steps towards ‘drop-in’ biofuels: focusing on metabolic pathways. United Kingdom. doi:
Jiang, Wen, Gu, Pengfei, and Zhang, Fuzhong. Mon . "Steps towards ‘drop-in’ biofuels: focusing on metabolic pathways". United Kingdom. doi:
title = {Steps towards ‘drop-in’ biofuels: focusing on metabolic pathways},
author = {Jiang, Wen and Gu, Pengfei and Zhang, Fuzhong},
abstractNote = {},
doi = {10.1016/j.copbio.2017.10.010},
journal = {Current Opinion in Biotechnology},
number = C,
volume = 53,
place = {United Kingdom},
year = {2018},
month = {10}

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Works referenced in this record:

Production of medium chain length fatty alcohols from glucose in Escherichia coli
journal, November 2013

Improving fatty acids production by engineering dynamic pathway regulation and metabolic control
journal, July 2014

  • Xu, P.; Li, L.; Zhang, F.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 31
  • DOI: 10.1073/pnas.1406401111

Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals
journal, August 2011

  • Dellomonaco, Clementina; Clomburg, James M.; Miller, Elliot N.
  • Nature, Vol. 476, Issue 7360, p. 355-359
  • DOI: 10.1038/nature10333

Engineering fungal de novo fatty acid synthesis for short chain fatty acid production
journal, March 2017

  • Gajewski, Jan; Pavlovic, Renata; Fischer, Manuel
  • Nature Communications, Vol. 8, Issue 1
  • DOI: 10.1038/ncomms14650

Engineering Microbial Metabolite Dynamics and Heterogeneity
journal, September 2017

  • Schmitz, Alexander C.; Hartline, Christopher J.; Zhang, Fuzhong
  • Biotechnology Journal, Vol. 12, Issue 10
  • DOI: 10.1002/biot.201700422

Precise precursor rebalancing for isoprenoids production by fine control of gapA expression in Escherichia coli
journal, November 2016

Production of anteiso-branched fatty acids in Escherichia coli; next generation biofuels with improved cold-flow properties
journal, November 2014

Metabolic variability in bioprocessing: implications of microbial phenotypic heterogeneity
journal, December 2014

Functional screening of aldehyde decarbonylases for long-chain alkane production by Saccharomyces cerevisiae
journal, May 2017

  • Kang, Min-Kyoung; Zhou, Yongjin J.; Buijs, Nicolaas A.
  • Microbial Cell Factories, Vol. 16, Issue 1
  • DOI: 10.1186/s12934-017-0683-z

Leveraging microbial biosynthetic pathways for the generation of ‘drop-in’ biofuels
journal, June 2017

Manipulation of GES and ERG20 for geraniol overproduction in Saccharomyces cerevisiae
journal, May 2017

Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
journal, April 2016

Microbial engineering for the production of advanced biofuels
journal, August 2012

  • Peralta-Yahya, Pamela P.; Zhang, Fuzhong; del Cardayre, Stephen B.
  • Nature, Vol. 488, Issue 7411
  • DOI: 10.1038/nature11478

Development of an orthogonal fatty acid biosynthesis system in E. coli for oleochemical production
journal, July 2015

Terpene synthases are widely distributed in bacteria
journal, December 2014

  • Yamada, Yuuki; Kuzuyama, Tomohisa; Komatsu, Mamoru
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 3
  • DOI: 10.1073/pnas.1422108112

Enhancing fatty acid production by the expression of the regulatory transcription factor FadR
journal, November 2012

Organic municipal solid waste (MSW) as feedstock for biodiesel production: A financial feasibility analysis
journal, February 2016

Metabolomics-driven approach to solving a CoA imbalance for improved 1-butanol production in Escherichia coli
journal, May 2017

Saturated mutagenesis of ketoisovalerate decarboxylase V461 enabled specific synthesis of 1-pentanol via the ketoacid elongation cycle
journal, September 2017

Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli
journal, January 2017

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
journal, January 2008

  • Atsumi, Shota; Hanai, Taizo; Liao, James C.
  • Nature, Vol. 451, Issue 7174, p. 86-89
  • DOI: 10.1038/nature06450

Engineering Escherichia coli to produce branched-chain fatty acids in high percentages
journal, November 2016

Quantifying cellular capacity identifies gene expression designs with reduced burden
journal, April 2015

  • Ceroni, Francesca; Algar, Rhys; Stan, Guy-Bart
  • Nature Methods, Vol. 12, Issue 5
  • DOI: 10.1038/nmeth.3339

A simple method to control glycolytic flux for the design of an optimal cell factory
journal, June 2017

Engineering modular polyketide synthases for production of biofuels and industrial chemicals
journal, April 2018

Negative Feedback Regulation of Fatty Acid Production Based on a Malonyl-CoA Sensor–Actuator
journal, January 2014

  • Liu, Di; Xiao, Yi; Evans, Bradley S.
  • ACS Synthetic Biology, Vol. 4, Issue 2
  • DOI: 10.1021/sb400158w

3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation
journal, January 2010

  • Connor, Michael R.; Cann, Anthony F.; Liao, James C.
  • Applied Microbiology and Biotechnology, Vol. 86, Issue 4
  • DOI: 10.1007/s00253-009-2401-1

Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. coli
journal, August 2014

Identification of a highly efficient stationary phase promoter in Bacillus subtilis
journal, December 2015

  • Yu, Xiaoxia; Xu, Jiangtao; Liu, Xiaoqing
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep18405

Microbial production of short-chain alkanes
journal, September 2013

Synthesis of medium chain length fatty acid ethyl esters in engineered Escherichia coli using endogenously produced medium chain fatty acids
journal, July 2013

Expanding ester biosynthesis in Escherichia coli
journal, March 2014

  • Rodriguez, Gabriel M.; Tashiro, Yohei; Atsumi, Shota
  • Nature Chemical Biology, Vol. 10, Issue 4
  • DOI: 10.1038/nchembio.1476

Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media
journal, June 2014

  • Willrodt, Christian; David, Christian; Cornelissen, Sjef
  • Biotechnology Journal, Vol. 9, Issue 8
  • DOI: 10.1002/biot.201400023

Exploiting nongenetic cell-to-cell variation for enhanced biosynthesis
journal, March 2016

  • Xiao, Yi; Bowen, Christopher H.; Liu, Di
  • Nature Chemical Biology, Vol. 12, Issue 5
  • DOI: 10.1038/nchembio.2046

Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit
journal, February 2017

  • Gupta, Apoorv; Reizman, Irene M. Brockman; Reisch, Christopher R.
  • Nature Biotechnology, Vol. 35, Issue 3
  • DOI: 10.1038/nbt.3796

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator
journal, June 2017

  • Ajjawi, Imad; Verruto, John; Aqui, Moena
  • Nature Biotechnology, Vol. 35, Issue 7
  • DOI: 10.1038/nbt.3865

Tailored fatty acid synthesis via dynamic control of fatty acid elongation
journal, June 2013

  • Torella, J. P.; Ford, T. J.; Kim, S. N.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 28
  • DOI: 10.1073/pnas.1307129110

Metabolic engineering for the high-yield production of isoprenoid-based C5 alcohols in E. coli
journal, June 2015

  • George, Kevin W.; Thompson, Mitchell G.; Kang, Aram
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep11128

Clostridium kluyverii, an Organism Concerned in the Formation of Caproic Acid from Ethyl Alcohol
journal, January 1942

A Synthetic Recursive “+1” Pathway for Carbon Chain Elongation
journal, February 2012

  • Marcheschi, Ryan J.; Li, Han; Zhang, Kechun
  • ACS Chemical Biology, Vol. 7, Issue 4
  • DOI: 10.1021/cb200313e

Energy- and carbon-efficient synthesis of functionalized small molecules in bacteria using non-decarboxylative Claisen condensation reactions
journal, April 2016

  • Cheong, Seokjung; Clomburg, James M.; Gonzalez, Ramon
  • Nature Biotechnology, Vol. 34, Issue 5
  • DOI: 10.1038/nbt.3505

Toward aldehyde and alkane production by removing aldehyde reductase activity in Escherichia coli
journal, September 2014

Development of an E. coli strain for one-pot biofuel production from ionic liquid pretreated cellulose and switchgrass
journal, January 2016

  • Frederix, Marijke; Mingardon, Florence; Hu, Matthew
  • Green Chemistry, Vol. 18, Issue 15
  • DOI: 10.1039/C6GC00642F

Enhanced production of branched-chain fatty acids by replacing β-ketoacyl-(acyl-carrier-protein) synthase III (FabH): Enhanced Production of Branched-Chain Fatty Acids
journal, April 2015

  • Jiang, Wen; Jiang, Yanfang; Bentley, Gayle J.
  • Biotechnology and Bioengineering, Vol. 112, Issue 8
  • DOI: 10.1002/bit.25583

Engineering Escherichia coli membrane phospholipid head distribution improves tolerance and production of biorenewables
journal, November 2017

Production of jet fuel precursor monoterpenoids from engineered Escherichia coli : Production of Jet Fuel Precursor Monoterpenoids
journal, May 2017

  • Mendez-Perez, Daniel; Alonso-Gutierrez, Jorge; Hu, Qijun
  • Biotechnology and Bioengineering, Vol. 114, Issue 8
  • DOI: 10.1002/bit.26296

Production of 2-methyl-1-butanol in engineered Escherichia coli
journal, August 2008

Cyanobacterial metabolic engineering for biofuel and chemical production
journal, December 2016

  • Oliver, Neal J.; Rabinovitch-Deere, Christine A.; Carroll, Austin L.
  • Current Opinion in Chemical Biology, Vol. 35
  • DOI: 10.1016/j.cbpa.2016.08.023

Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli
journal, March 2011

  • Shen, Claire R.; Lan, Ethan I.; Dekishima, Yasumasa
  • Applied and Environmental Microbiology, Vol. 77, Issue 9, p. 2905-2915
  • DOI: 10.1128/AEM.03034-10

Bacterial terpene cyclases
journal, January 2016

Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways
journal, November 2008

Metabolic engineering of Saccharomyces cerevisiae to produce 1-hexadecanol from xylose
journal, February 2016

Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production
journal, January 2014

  • Blazeck, John; Hill, Andrew; Liu, Leqian
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms4131