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Title: Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing

Abstract

Lignin is the number one cause of lignocellulosic biomass recalcitrance to industrial enzymatic hydrolysis. By partially replacing the traditional lignin monomers by alternative ones, lignin extractability can be enhanced. To design a lignin that is easier to degrade under alkaline conditions, curcumin (diferuloylmethane) was produced in the model plant Arabidopsis thaliana via simultaneous expression of the turmeric ( Curcuma longa) genes DIKETIDE-CoA SYNTHASE (DCS) and CURCUMIN SYNTHASE 2 (CURS2). The transgenic plants produced a plethora of curcumin- and phenylpentanoid-derived compounds with no negative impact on growth. Catalytic hydrogenolysis gave evidence that both curcumin and phenylpentanoids were incorporated into the lignifying cell wall, thereby greatly increasing saccharification efficiency after alkaline pretreatment of the transgenic lines by 14–24% as compared with the wild type. These results reflect that non-native monomers can be synthesized and incorporated into the lignin polymer in plants to enhance their biomass processing efficiency.

Authors:
 [1];  [1]; ORCiD logo [1]; ORCiD logo [1];  [2];  [1];  [2];  [3]; ORCiD logo [3]; ORCiD logo [4];  [4]; ORCiD logo [3];  [2]; ORCiD logo [2]
  1. Ghent Univ. (Belgium); VIB Center for Plant Systems Biology, Ghent (Belgium)
  2. Ghent Univ. (Belgium); VIB Center for Plant Systems Biology, Ghent (Belgium); VIB Metabolomics Core, Ghent (Belgium)
  3. Univ. of Wisconsin, Madison, WI (United States); USDOE Great Lakes Bioenergy Research Center, Madison, WI (United States)
  4. KU Leuven, Heverlee (Belgium)
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1548319
Grant/Contract Number:  
SC0018409
Resource Type:
Accepted Manuscript
Journal Name:
Nature Plants
Additional Journal Information:
Journal Volume: 5; Journal Issue: 2; Journal ID: ISSN 2055-0278
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English

Citation Formats

Oyarce, Paula, De Meester, Barbara, Fonseca, Fernando, de Vries, Lisanne, Goeminne, Geert, Pallidis, Andreas, De Rycke, Riet, Tsuji, Yukiko, Li, Yanding, Van den Bosch, Sander, Sels, Bert, Ralph, John, Vanholme, Ruben, and Boerjan, Wout. Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing. United States: N. p., 2019. Web. doi:10.1038/s41477-018-0350-3.
Oyarce, Paula, De Meester, Barbara, Fonseca, Fernando, de Vries, Lisanne, Goeminne, Geert, Pallidis, Andreas, De Rycke, Riet, Tsuji, Yukiko, Li, Yanding, Van den Bosch, Sander, Sels, Bert, Ralph, John, Vanholme, Ruben, & Boerjan, Wout. Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing. United States. doi:10.1038/s41477-018-0350-3.
Oyarce, Paula, De Meester, Barbara, Fonseca, Fernando, de Vries, Lisanne, Goeminne, Geert, Pallidis, Andreas, De Rycke, Riet, Tsuji, Yukiko, Li, Yanding, Van den Bosch, Sander, Sels, Bert, Ralph, John, Vanholme, Ruben, and Boerjan, Wout. Mon . "Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing". United States. doi:10.1038/s41477-018-0350-3.
@article{osti_1548319,
title = {Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing},
author = {Oyarce, Paula and De Meester, Barbara and Fonseca, Fernando and de Vries, Lisanne and Goeminne, Geert and Pallidis, Andreas and De Rycke, Riet and Tsuji, Yukiko and Li, Yanding and Van den Bosch, Sander and Sels, Bert and Ralph, John and Vanholme, Ruben and Boerjan, Wout},
abstractNote = {Lignin is the number one cause of lignocellulosic biomass recalcitrance to industrial enzymatic hydrolysis. By partially replacing the traditional lignin monomers by alternative ones, lignin extractability can be enhanced. To design a lignin that is easier to degrade under alkaline conditions, curcumin (diferuloylmethane) was produced in the model plant Arabidopsis thaliana via simultaneous expression of the turmeric (Curcuma longa) genes DIKETIDE-CoA SYNTHASE (DCS) and CURCUMIN SYNTHASE 2 (CURS2). The transgenic plants produced a plethora of curcumin- and phenylpentanoid-derived compounds with no negative impact on growth. Catalytic hydrogenolysis gave evidence that both curcumin and phenylpentanoids were incorporated into the lignifying cell wall, thereby greatly increasing saccharification efficiency after alkaline pretreatment of the transgenic lines by 14–24% as compared with the wild type. These results reflect that non-native monomers can be synthesized and incorporated into the lignin polymer in plants to enhance their biomass processing efficiency.},
doi = {10.1038/s41477-018-0350-3},
journal = {Nature Plants},
number = 2,
volume = 5,
place = {United States},
year = {2019},
month = {1}
}

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