Production of lipid and carbohydrate from microalgae without compromising biomass productivities: Role of Ca and Mg
Abstract
We report the cultivation of Chlorella sorokiniana str. SLA-04 in media containing trace amounts of Ca and Mg. The differences in productivities of biomass, lipids and carbohydrates were assessed relative to cultures grown in standard media (BG-11) that contain approximately 8 × higher concentration of Ca and 30 × higher concentration of Mg. Culture performance in N-limited standard media was also investigated. In addition to growth and accumulation of storage products (lipid and carbohydrate), we measured the utilization of N, Ca and Mg and monitored changes in cell size and photosynthetic activity. Our results showed that limitation of Ca or Mg did not inhibit cell replication and culture growth. On the contrary, Ca-limited (CaL) cultures had ~30% higher biomass productivity relative to the control with excessive nutrients possibly due to improvement in cell wall flexibility and cell division. We also observed that CaL and Mg-limited (MgL) cultures had nearly 3-fold higher lipid concentration (measured as fatty acid methyl ester) and 50% higher carbohydrate concentration than the nutrient excess control cultures. Simultaneous culture growth and lipid accumulation in CaL and MgL cultures suggest that de novo synthesis was the primary mechanism for lipid accumulation in Ca/Mg-limited media. Overall, our study demonstratesmore »
- Authors:
-
[1];
[1]
- Univ. of Toledo, OH (United States)
- Univ. of Toledo, OH (United States); Univ. of Southampton (United Kingdom)
- Publication Date:
- Research Org.:
- Univ. of Toledo, OH (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE
- OSTI Identifier:
- 1613321
- Alternate Identifier(s):
- OSTI ID: 1548192
- Grant/Contract Number:
- EE0005993
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Renewable Energy
- Additional Journal Information:
- Journal Volume: 127; Journal ID: ISSN 0960-1481
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Chlorella sorokiniana; cultivation; micronutrient; biofuel; nutrient limitation/deficiency; biorefinery
Citation Formats
Hanifzadeh, MohammadMatin, Garcia, Elena Cerdan, and Viamajala, Sridhar. Production of lipid and carbohydrate from microalgae without compromising biomass productivities: Role of Ca and Mg. United States: N. p., 2018.
Web. doi:10.1016/j.renene.2018.05.012.
Hanifzadeh, MohammadMatin, Garcia, Elena Cerdan, & Viamajala, Sridhar. Production of lipid and carbohydrate from microalgae without compromising biomass productivities: Role of Ca and Mg. United States. https://doi.org/10.1016/j.renene.2018.05.012
Hanifzadeh, MohammadMatin, Garcia, Elena Cerdan, and Viamajala, Sridhar. Sat .
"Production of lipid and carbohydrate from microalgae without compromising biomass productivities: Role of Ca and Mg". United States. https://doi.org/10.1016/j.renene.2018.05.012. https://www.osti.gov/servlets/purl/1613321.
@article{osti_1613321,
title = {Production of lipid and carbohydrate from microalgae without compromising biomass productivities: Role of Ca and Mg},
author = {Hanifzadeh, MohammadMatin and Garcia, Elena Cerdan and Viamajala, Sridhar},
abstractNote = {We report the cultivation of Chlorella sorokiniana str. SLA-04 in media containing trace amounts of Ca and Mg. The differences in productivities of biomass, lipids and carbohydrates were assessed relative to cultures grown in standard media (BG-11) that contain approximately 8 × higher concentration of Ca and 30 × higher concentration of Mg. Culture performance in N-limited standard media was also investigated. In addition to growth and accumulation of storage products (lipid and carbohydrate), we measured the utilization of N, Ca and Mg and monitored changes in cell size and photosynthetic activity. Our results showed that limitation of Ca or Mg did not inhibit cell replication and culture growth. On the contrary, Ca-limited (CaL) cultures had ~30% higher biomass productivity relative to the control with excessive nutrients possibly due to improvement in cell wall flexibility and cell division. We also observed that CaL and Mg-limited (MgL) cultures had nearly 3-fold higher lipid concentration (measured as fatty acid methyl ester) and 50% higher carbohydrate concentration than the nutrient excess control cultures. Simultaneous culture growth and lipid accumulation in CaL and MgL cultures suggest that de novo synthesis was the primary mechanism for lipid accumulation in Ca/Mg-limited media. Overall, our study demonstrates that micronutrient optimization, in addition to optimization of macronutrients, could significantly improve microalgal biorefinery yields.},
doi = {10.1016/j.renene.2018.05.012},
journal = {Renewable Energy},
number = ,
volume = 127,
place = {United States},
year = {Sat May 05 00:00:00 EDT 2018},
month = {Sat May 05 00:00:00 EDT 2018}
}
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