Metabolomics and Isotope Tracing

Jang, Cholsoon; Chen, Li; Rabinowitz, Joshua D.

doi:10.1016/j.cell.2018.03.055

Title: Metabolomics and Isotope Tracing

Abstract

We report great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. Additionally, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.

Authors:: Jang, Cholsoon; Chen, Li; Rabinowitz, Joshua D.

Publication Date:: Tue May 01 00:00:00 EDT 2018

Research Org.:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1548181

Alternate Identifier(s):: OSTI ID: 1487276

Grant/Contract Number:: SC0018420; SC0018260

Resource Type:: Published Article

Journal Name:: Cell

Additional Journal Information:: Journal Name: Cell Journal Volume: 173 Journal Issue: 4; Journal ID: ISSN 0092-8674

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Jang, Cholsoon, Chen, Li, and Rabinowitz, Joshua D. Metabolomics and Isotope Tracing.  United States: N. p., 2018. 
Web.  doi:10.1016/j.cell.2018.03.055.

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                    Jang, Cholsoon, Chen, Li, & Rabinowitz, Joshua D. Metabolomics and Isotope Tracing.  United States.  https://doi.org/10.1016/j.cell.2018.03.055

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                    Jang, Cholsoon, Chen, Li, and Rabinowitz, Joshua D. Tue .  
"Metabolomics and Isotope Tracing".  United States.  https://doi.org/10.1016/j.cell.2018.03.055.

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@article{osti_1548181,

  title        = {Metabolomics and Isotope Tracing},

  author       = {Jang, Cholsoon and Chen, Li and Rabinowitz, Joshua D.},

  abstractNote = {We report great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. Additionally, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.},

  doi          = {10.1016/j.cell.2018.03.055},

  journal      = {Cell},

  number       = 4,

  volume       = 173,

  place        = {United States},

  year         = {Tue May 01 00:00:00 EDT 2018},

  month        = {Tue May 01 00:00:00 EDT 2018}

}

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Title: Metabolomics and Isotope Tracing

Abstract

Citation Formats

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