Hachimoji DNA and RNA: A genetic system with eight building blocks
Abstract
We report DNA- and RNA-like systems built from eight nucleotide “letters” (hence the name “hachimoji”) that form four orthogonal pairs. These synthetic systems meet the structural requirements needed to support Darwinian evolution, including a polyelectrolyte backbone, predictable thermodynamic stability, and stereoregular building blocks that fit a Schrödinger aperiodic crystal. Measured thermodynamic parameters predict the stability of hachimoji duplexes, allowing hachimoji DNA to increase the information density of natural terran DNA. Three crystal structures show that the synthetic building blocks do not perturb the aperiodic crystal seen in the DNA double helix. Hachimoji DNA was then transcribed to give hachimoji RNA in the form of a functioning fluorescent hachimoji aptamer. These results expand the scope of molecular structures that might support life, including life throughout the cosmos.
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1547949
- Grant/Contract Number:
- AC02-76SF00515
- Resource Type:
- Published Article
- Journal Name:
- Science
- Additional Journal Information:
- Journal Name: Science Journal Volume: 363 Journal Issue: 6429; Journal ID: ISSN 0036-8075
- Publisher:
- American Association for the Advancement of Science (AAAS)
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Hoshika, Shuichi, Leal, Nicole A., Kim, Myong-Jung, Kim, Myong-Sang, Karalkar, Nilesh B., Kim, Hyo-Joong, Bates, Alison M., Watkins, Jr., Norman E., SantaLucia, Holly A., Meyer, Adam J., DasGupta, Saurja, Piccirilli, Joseph A., Ellington, Andrew D., SantaLucia, Jr., John, Georgiadis, Millie M., and Benner, Steven A. Hachimoji DNA and RNA: A genetic system with eight building blocks. United States: N. p., 2019.
Web. doi:10.1126/science.aat0971.
Hoshika, Shuichi, Leal, Nicole A., Kim, Myong-Jung, Kim, Myong-Sang, Karalkar, Nilesh B., Kim, Hyo-Joong, Bates, Alison M., Watkins, Jr., Norman E., SantaLucia, Holly A., Meyer, Adam J., DasGupta, Saurja, Piccirilli, Joseph A., Ellington, Andrew D., SantaLucia, Jr., John, Georgiadis, Millie M., & Benner, Steven A. Hachimoji DNA and RNA: A genetic system with eight building blocks. United States. https://doi.org/10.1126/science.aat0971
Hoshika, Shuichi, Leal, Nicole A., Kim, Myong-Jung, Kim, Myong-Sang, Karalkar, Nilesh B., Kim, Hyo-Joong, Bates, Alison M., Watkins, Jr., Norman E., SantaLucia, Holly A., Meyer, Adam J., DasGupta, Saurja, Piccirilli, Joseph A., Ellington, Andrew D., SantaLucia, Jr., John, Georgiadis, Millie M., and Benner, Steven A. Thu .
"Hachimoji DNA and RNA: A genetic system with eight building blocks". United States. https://doi.org/10.1126/science.aat0971.
@article{osti_1547949,
title = {Hachimoji DNA and RNA: A genetic system with eight building blocks},
author = {Hoshika, Shuichi and Leal, Nicole A. and Kim, Myong-Jung and Kim, Myong-Sang and Karalkar, Nilesh B. and Kim, Hyo-Joong and Bates, Alison M. and Watkins, Jr., Norman E. and SantaLucia, Holly A. and Meyer, Adam J. and DasGupta, Saurja and Piccirilli, Joseph A. and Ellington, Andrew D. and SantaLucia, Jr., John and Georgiadis, Millie M. and Benner, Steven A.},
abstractNote = {We report DNA- and RNA-like systems built from eight nucleotide “letters” (hence the name “hachimoji”) that form four orthogonal pairs. These synthetic systems meet the structural requirements needed to support Darwinian evolution, including a polyelectrolyte backbone, predictable thermodynamic stability, and stereoregular building blocks that fit a Schrödinger aperiodic crystal. Measured thermodynamic parameters predict the stability of hachimoji duplexes, allowing hachimoji DNA to increase the information density of natural terran DNA. Three crystal structures show that the synthetic building blocks do not perturb the aperiodic crystal seen in the DNA double helix. Hachimoji DNA was then transcribed to give hachimoji RNA in the form of a functioning fluorescent hachimoji aptamer. These results expand the scope of molecular structures that might support life, including life throughout the cosmos.},
doi = {10.1126/science.aat0971},
journal = {Science},
number = 6429,
volume = 363,
place = {United States},
year = {Thu Feb 21 00:00:00 EST 2019},
month = {Thu Feb 21 00:00:00 EST 2019}
}
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