Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Kellock, Miriam; Maaheimo, Hannu; Marjamaa, Kaisa; Rahikainen, Jenni; Zhang, Heng; Holopainen-Mantila, Ulla; Ralph, John; Tamminen, Tarja; Felby, Claus; Kruus, Kristiina

doi:10.1016/j.biortech.2019.02.051

Title: Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Journal Article · 10 February 2019 · Bioresource Technology

DOI: https://doi.org/10.1016/j.biortech.2019.02.051 · OSTI ID:1546998

^[1]; Maaheimo, Hannu ^[2]; Marjamaa, Kaisa ^[2];

^[2]; Zhang, Heng ^[3]; Holopainen-Mantila, Ulla ^[2]; Ralph, John ^[4]; Tamminen, Tarja ^[2]; Felby, Claus ^[3];

^[2]

VTT Technical Research Centre of Finland Ltd, VTT (Finland); Aalto University Dean's Unit of the School of Chemical Engineering
VTT Technical Research Centre of Finland Ltd, VTT (Finland)
Univ. of Copenhagen, Frederiksberg C (Denmark)
Univ. of Wisconsin, Madison, WI (United States)

Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States). Great Lakes Bioenergy Research Center

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: SC0018409

OSTI ID:: 1546998

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: C Vol. 280; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (34)

An α-glucuronidase of Schizophyllum commune acting on polymeric xylan Tenkanen, Maija; Siika-aho, Matti Journal of Biotechnology, Vol. 78, Issue 2 https://doi.org/10.1016/s0168-1656(99)00240-0	journal	March 2000
Predicting Enzyme Adsorption to Lignin Films by Calculating Enzyme Surface Hydrophobicity Sammond, Deanne W.; Yarbrough, John M.; Mansfield, Elisabeth Journal of Biological Chemistry, Vol. 289, Issue 30 https://doi.org/10.1074/jbc.m114.573642	journal	May 2014
Ball Milling’s Effect on Pine Milled Wood Lignin’s Structure and Molar Mass Zinovyev, Grigory; Sumerskii, Ivan; Rosenau, Thomas Molecules, Vol. 23, Issue 9 https://doi.org/10.3390/molecules23092223	journal	September 2018
A New Type of Rearrangement in the Lignin Field Nimz, H. Angewandte Chemie International Edition in English, Vol. 5, Issue 9 https://doi.org/10.1002/anie.196608431	journal	September 1966
Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment Donohoe, Bryon S.; Decker, Stephen R.; Tucker, Melvin P. Biotechnology and Bioengineering, Vol. 101, Issue 5 https://doi.org/10.1002/bit.21959	journal	December 2008
Inhibition of enzymatic hydrolysis by residual lignins from softwood-study of enzyme binding and inactivation on lignin-rich surface Rahikainen, Jenni; Mikander, Saara; Marjamaa, Kaisa Biotechnology and Bioengineering, Vol. 108, Issue 12 https://doi.org/10.1002/bit.23242	journal	June 2011
Correlation between lignin physicochemical properties and inhibition to enzymatic hydrolysis of cellulose: Lignin Properties and Enzymatic Hydrolysis Yang, Qiang; Pan, Xuejun Biotechnology and Bioengineering, Vol. 113, Issue 6 https://doi.org/10.1002/bit.25903	journal	December 2015
Pretreatment-Catalyst effects and the combined severity parameter Chum, Helena L.; Johnson, David K.; Black, Stuart K. Applied Biochemistry and Biotechnology, Vol. 24-25, Issue 1 https://doi.org/10.1007/BF02920229	journal	March 1990
Efficient cellulase production by Trichoderma reesei in continuous cultivation on lactose medium with a computer-controlled feeding strategy Bailey, M. J.; T�htiharju, J. Applied Microbiology and Biotechnology, Vol. 62, Issue 2-3 https://doi.org/10.1007/s00253-003-1276-9	journal	August 2003
Hydroxycinnamates in lignification Ralph, John Phytochemistry Reviews, Vol. 9, Issue 1, p. 65-83 https://doi.org/10.1007/s11101-009-9141-9	journal	August 2009
Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d ₆ Kim, Hoon; Ralph, John; Akiyama, Takuya BioEnergy Research, Vol. 1, Issue 1, p. 56-66 https://doi.org/10.1007/s12155-008-9004-z	journal	March 2008
An α-glucuronidase of Schizophyllum commune acting on polymeric xylan Tenkanen, Maija; Siika-aho, Matti Journal of Biotechnology, Vol. 78, Issue 2 https://doi.org/10.1016/S0168-1656(99)00240-0	journal	March 2000
Lignin depolymerization/repolymerization and its critical role for delignification of aspen wood by steam explosion Li, Jiebing; Henriksson, Gunnar; Gellerstedt, Göran Bioresource Technology, Vol. 98, Issue 16 https://doi.org/10.1016/j.biortech.2006.10.018	journal	November 2007
The isolation, characterization and effect of lignin isolated from steam pretreated Douglas-fir on the enzymatic hydrolysis of cellulose Nakagame, Seiji; Chandra, Richard P.; Kadla, John F. Bioresource Technology, Vol. 102, Issue 6 https://doi.org/10.1016/j.biortech.2010.12.082	journal	March 2011
Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption Rahikainen, Jenni L.; Martin-Sampedro, Raquel; Heikkinen, Harri Bioresource Technology, Vol. 133 https://doi.org/10.1016/j.biortech.2013.01.075	journal	April 2013
Lignin-derived inhibition of monocomponent cellulases and a xylanase in the hydrolysis of lignocellulosics Kellock, Miriam; Rahikainen, Jenni; Marjamaa, Kaisa Bioresource Technology, Vol. 232 https://doi.org/10.1016/j.biortech.2017.01.072	journal	May 2017
Cellulase–lignin interactions—The role of carbohydrate-binding module and pH in non-productive binding Rahikainen, Jenni Liisa; Evans, James David; Mikander, Saara Enzyme and Microbial Technology, Vol. 53, Issue 5 https://doi.org/10.1016/j.enzmictec.2013.07.003	journal	October 2013
Lignocellulose pretreatment severity – relating pH to biomatrix opening Pedersen, Mads; Meyer, Anne S. New Biotechnology, Vol. 27, Issue 6 https://doi.org/10.1016/j.nbt.2010.05.003	journal	December 2010
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Miller, G. L. Analytical Chemistry, Vol. 31, Issue 3 https://doi.org/10.1021/ac60147a030	journal	March 1959
Impact of Steam Explosion on the Wheat Straw Lignin Structure Studied by Solution-State Nuclear Magnetic Resonance and Density Functional Methods Heikkinen, Harri; Elder, Thomas; Maaheimo, Hannu Journal of Agricultural and Food Chemistry, Vol. 62, Issue 43 https://doi.org/10.1021/jf504622j	journal	October 2014
Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp Suurnäkki, A.; Tenkanen, M.; Siika-aho, M. Cellulose, Vol. 7, Issue 2, p. 189-209 https://doi.org/10.1023/A:1009280109519	journal	June 2000
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Laemmli, U. K. Nature, Vol. 227, Issue 5259, p. 680-685 https://doi.org/10.1038/227680a0	journal	August 1970
Whole plant cell wall characterization using solution-state 2D NMR Mansfield, Shawn D.; Kim, Hoon; Lu, Fachuang Nature Protocols, Vol. 7, Issue 9 https://doi.org/10.1038/nprot.2012.064	journal	August 2012
The strong association of condensed phenolic moieties in isolated lignins with their inhibition of enzymatic hydrolysis Sun, Shaolong; Huang, Yang; Sun, Runcang Green Chemistry, Vol. 18, Issue 15 https://doi.org/10.1039/C6GC00685J	journal	January 2016
Chemical transformations of Populus trichocarpa during dilute acid pretreatment Cao, Shilin; Pu, Yunqiao; Studer, Michael RSC Advances, Vol. 2, Issue 29 https://doi.org/10.1039/c2ra22045h	journal	January 2012
Predicting Enzyme Adsorption to Lignin Films by Calculating Enzyme Surface Hydrophobicity Sammond, Deanne W.; Yarbrough, John M.; Mansfield, Elisabeth Journal of Biological Chemistry, Vol. 289, Issue 30 https://doi.org/10.1074/jbc.M114.573642	journal	May 2014
Tricin, a Flavonoid Monomer in Monocot Lignification Lan, Wu; Lu, Fachuang; Regner, Matthew Plant Physiology, Vol. 167, Issue 4 https://doi.org/10.1104/pp.114.253757	journal	February 2015
Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content Eloy, Nubia B.; Voorend, Wannes; Lan, Wu Plant Physiology, Vol. 173, Issue 2 https://doi.org/10.1104/pp.16.01108	journal	December 2016
Assessment of leaf/stem ratio in wheat straw feedstock and impact on enzymatic conversion Zhang, Heng; Fangel, Jonatan U.; Willats, William G. T. GCB Bioenergy, Vol. 6, Issue 1 https://doi.org/10.1111/gcbb.12060	journal	April 2013
Tricin-lignins: occurrence and quantitation of tricin in relation to phylogeny Lan, Wu; Rencoret, Jorge; Lu, Fachuang The Plant Journal, Vol. 88, Issue 6 https://doi.org/10.1111/tpj.13315	journal	November 2016
The synergistic action of accessory enzymes enhances the hydrolytic potential of a “cellulase mixture” but is highly substrate specific Hu, Jinguang; Arantes, Valdeir; Pribowo, Amadeus Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-112	journal	January 2013
Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments Pu, Yunqiao; Hu, Fan; Huang, Fang Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-15	journal	January 2013
2D NMR characterization of wheat straw residual lignin after dilute acid pretreatment with different severities Jensen, Anders; Cabrera, Yohanna; Hsieh, Chia-Wen Holzforschung, Vol. 71, Issue 6 https://doi.org/10.1515/hf-2016-0112	journal	June 2017
Ball Milling’s Effect on Pine Milled Wood Lignin’s Structure and Molar Mass Zinovyev, Grigory; Sumerskii, Ivan; Rosenau, Thomas Molecules, Vol. 23, Issue 9 https://doi.org/10.3390/molecules23092223	journal	September 2018

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