Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin
Abstract
Bacteriorhodopsin (bR) is a light-driven proton pump. The primary photochemical event upon light absorption is isomerization of the retinal chromophore. Here we used time-resolved crystallography at an X-ray free-electron laser to follow the structural changes in multiphoton-excited bR from 250 femtoseconds to 10 picoseconds. Quantum chemistry and ultrafast spectroscopy were used to identify a sequential two-photon absorption process, leading to excitation of a tryptophan residue flanking the retinal chromophore, as a first manifestation of multiphoton effects. We resolve distinct stages in the structural dynamics of the all-trans retinal in photoexcited bR to a highly twisted 13-cis conformation. Other active site sub-picosecond rearrangements include correlated vibrational motions of the electronically excited retinal chromophore, the surrounding amino acids and water molecules as well as their hydrogen bonding network. These results show that this extended photo-active network forms an electronically and vibrationally coupled system in bR, and most likely in all retinal proteins.
- Authors:
-
more »
- Max-Planck-Inst. für Medizinische Forschung, Heidelberg (Germany)
- Univ. Grenoble Alpes, Grenoble (France)
- Freie Univ. Berlin, Berlin (Germany)
- SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
- Max-Planck-Inst. für Molekulare Physiologie, Dortmund (Germany)
- Univ. de Strasbourg-CNRS, Strasbourg (France)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1546794
- Grant/Contract Number:
- AC02-76SF00515; ANR-17-CE11-0018-01
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS
Citation Formats
Nass Kovacs, Gabriela, Colletier, Jacques-Philippe, Grünbein, Marie Luise, Yang, Yang, Stensitzki, Till, Batyuk, Alexander, Carbajo, Sergio, Doak, R. Bruce, Ehrenberg, David, Foucar, Lutz, Gasper, Raphael, Gorel, Alexander, Hilpert, Mario, Kloos, Marco, Koglin, Jason E., Reinstein, Jochen, Roome, Christopher M., Schlesinger, Ramona, Seaberg, Matthew, Shoeman, Robert L., Stricker, Miriam, Boutet, Sébastien, Haacke, Stefan, Heberle, Joachim, Heyne, Karsten, Domratcheva, Tatiana, Barends, Thomas R. M., and Schlichting, Ilme. Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin. United States: N. p., 2019.
Web. doi:10.1038/s41467-019-10758-0.
Nass Kovacs, Gabriela, Colletier, Jacques-Philippe, Grünbein, Marie Luise, Yang, Yang, Stensitzki, Till, Batyuk, Alexander, Carbajo, Sergio, Doak, R. Bruce, Ehrenberg, David, Foucar, Lutz, Gasper, Raphael, Gorel, Alexander, Hilpert, Mario, Kloos, Marco, Koglin, Jason E., Reinstein, Jochen, Roome, Christopher M., Schlesinger, Ramona, Seaberg, Matthew, Shoeman, Robert L., Stricker, Miriam, Boutet, Sébastien, Haacke, Stefan, Heberle, Joachim, Heyne, Karsten, Domratcheva, Tatiana, Barends, Thomas R. M., & Schlichting, Ilme. Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin. United States. https://doi.org/10.1038/s41467-019-10758-0
Nass Kovacs, Gabriela, Colletier, Jacques-Philippe, Grünbein, Marie Luise, Yang, Yang, Stensitzki, Till, Batyuk, Alexander, Carbajo, Sergio, Doak, R. Bruce, Ehrenberg, David, Foucar, Lutz, Gasper, Raphael, Gorel, Alexander, Hilpert, Mario, Kloos, Marco, Koglin, Jason E., Reinstein, Jochen, Roome, Christopher M., Schlesinger, Ramona, Seaberg, Matthew, Shoeman, Robert L., Stricker, Miriam, Boutet, Sébastien, Haacke, Stefan, Heberle, Joachim, Heyne, Karsten, Domratcheva, Tatiana, Barends, Thomas R. M., and Schlichting, Ilme. Thu .
"Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin". United States. https://doi.org/10.1038/s41467-019-10758-0. https://www.osti.gov/servlets/purl/1546794.
@article{osti_1546794,
title = {Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin},
author = {Nass Kovacs, Gabriela and Colletier, Jacques-Philippe and Grünbein, Marie Luise and Yang, Yang and Stensitzki, Till and Batyuk, Alexander and Carbajo, Sergio and Doak, R. Bruce and Ehrenberg, David and Foucar, Lutz and Gasper, Raphael and Gorel, Alexander and Hilpert, Mario and Kloos, Marco and Koglin, Jason E. and Reinstein, Jochen and Roome, Christopher M. and Schlesinger, Ramona and Seaberg, Matthew and Shoeman, Robert L. and Stricker, Miriam and Boutet, Sébastien and Haacke, Stefan and Heberle, Joachim and Heyne, Karsten and Domratcheva, Tatiana and Barends, Thomas R. M. and Schlichting, Ilme},
abstractNote = {Bacteriorhodopsin (bR) is a light-driven proton pump. The primary photochemical event upon light absorption is isomerization of the retinal chromophore. Here we used time-resolved crystallography at an X-ray free-electron laser to follow the structural changes in multiphoton-excited bR from 250 femtoseconds to 10 picoseconds. Quantum chemistry and ultrafast spectroscopy were used to identify a sequential two-photon absorption process, leading to excitation of a tryptophan residue flanking the retinal chromophore, as a first manifestation of multiphoton effects. We resolve distinct stages in the structural dynamics of the all-trans retinal in photoexcited bR to a highly twisted 13-cis conformation. Other active site sub-picosecond rearrangements include correlated vibrational motions of the electronically excited retinal chromophore, the surrounding amino acids and water molecules as well as their hydrogen bonding network. These results show that this extended photo-active network forms an electronically and vibrationally coupled system in bR, and most likely in all retinal proteins.},
doi = {10.1038/s41467-019-10758-0},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {Thu Jul 18 00:00:00 EDT 2019},
month = {Thu Jul 18 00:00:00 EDT 2019}
}
Web of Science
Works referenced in this record:
Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
text, January 2015
- Nogly, Przemyslaw; James, Daniel; Wang, Dingjie
- ETH Zurich
CrystFEL : a software suite for snapshot serial crystallography
journal, March 2012
- White, Thomas A.; Kirian, Richard A.; Martin, Andrew V.
- Journal of Applied Crystallography, Vol. 45, Issue 2
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Apollo - University of Cambridge Repository
Excited-state reaction dynamics of bacteriorhodopsin studied by femtosecond spectroscopy
journal, February 1988
- Dobler, J.; Zinth, W.; Kaiser, W.
- Chemical Physics Letters, Vol. 144, Issue 2
Femtosecond Infrared Spectroscopy of Bacteriorhodopsin Chromophore Isomerization
journal, August 2002
- Herbst, J.
- Science, Vol. 297, Issue 5582
Primary Step in Bacteriorhodopsin Photosynthesis: Bond Stretch Rather than Angle Twist of Its Retinal Excited-State Structure
journal, September 1998
- Song, Li; El-Sayed, M. A.
- Journal of the American Chemical Society, Vol. 120, Issue 34
Functional electric field changes in photoactivated proteins revealed by ultrafast Stark spectroscopy of the Trp residues
journal, April 2009
- Leonard, J.; Portuondo-Campa, E.; Cannizzo, A.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 19
Structure-factor extrapolation using the scalar approximation: theory, applications and limitations
journal, September 2007
- Genick, Ulrich K.
- Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 10
Probing the Ultrafast Charge Translocation of Photoexcited Retinal in Bacteriorhodopsin
journal, August 2005
- Schenkl, S.
- Science, Vol. 309, Issue 5736
Measurements of the driving forces of bio-motors using the fluctuation theorem
journal, January 2012
- Hayashi, Kumiko; Tanigawara, Mizue; Kishikawa, Jun-ichi
- BIOPHYSICS, Vol. 8
The primary photoreaction of channelrhodopsin-1: wavelength dependent photoreactions induced by ground-state heterogeneity
journal, July 2015
- Stensitzki, Till; Muders, Vera; Schlesinger, Ramona
- Frontiers in Molecular Biosciences, Vol. 2
The Coherent X-ray Imaging instrument at the Linac Coherent Light Source
journal, April 2015
- Liang, Mengning; Williams, Garth J.; Messerschmidt, Marc
- Journal of Synchrotron Radiation, Vol. 22, Issue 3
Viscous hydrophilic injection matrices for serial crystallography
journal, May 2017
- Kovácsová, Gabriela; Grünbein, Marie Luise; Kloos, Marco
- IUCrJ, Vol. 4, Issue 4
Proton transfers in the bacteriorhodopsin photocycle
journal, August 2006
- Lanyi, Janos K.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1757, Issue 8
Retinal isomer ratio in dark-adapted purple membrane and bacteriorhodopsin monomers
journal, January 1989
- Scherrer, P.; Mathew, M. K.; Sperling, W.
- Biochemistry, Vol. 28, Issue 2
Resonant Two-Photon Excitation Pathways During Retinal-Isomerization in Bacteriorhodopsin
journal, January 2013
- Kraack, J. P.; Buckup, T.; Motzkus, M.
- EPJ Web of Conferences, Vol. 41
Comparing photoinduced vibrational coherences in bacteriorhodopsin and in native and locked retinal protonated Schiff bases
journal, November 2003
- Hou, Bixue; Friedman, Noga; Ottolenghi, Michael
- Chemical Physics Letters, Vol. 381, Issue 5-6
Excited-state dynamics of bacteriorhodopsin probed by broadband femtosecond fluorescence spectroscopy
journal, January 2005
- Schmidt, B.; Sobotta, C.; Heinz, B.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1706, Issue 1-2
Aborted double bicycle-pedal isomerization with hydrogen bond breaking is the primary event of bacteriorhodopsin proton pumping
journal, November 2010
- Altoe, P.; Cembran, A.; Olivucci, M.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 47
Extended multi-configuration quasi-degenerate perturbation theory: The new approach to multi-state multi-reference perturbation theory
journal, June 2011
- Granovsky, Alexander A.
- The Journal of Chemical Physics, Vol. 134, Issue 21
Energy transfer from retinal to amino acids — a time-resolved study of the ultraviolet emission of bacteriorhodopsin
journal, October 1986
- Polland, H. J.; Franz, M. A.; Zinth, W.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 851, Issue 3
Development of the Colle-Salvetti correlation-energy formula into a functional of the electron density
journal, January 1988
- Lee, Chengteh; Yang, Weitao; Parr, Robert G.
- Physical Review B, Vol. 37, Issue 2
Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
journal, August 2014
- Arnlund, David; Johansson, Linda C.; Wickstrand, Cecilia
- Nature Methods, Vol. 11, Issue 9
CASS—CFEL-ASG software suite
journal, October 2012
- Foucar, Lutz; Barty, Anton; Coppola, Nicola
- Computer Physics Communications, Vol. 183, Issue 10
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
Coherent control of the isomerization of retinal in bacteriorhodopsin in the high intensity regime
journal, February 2011
- Prokhorenko, Valentyn I.; Halpin, Alexei; Johnson, Philip J. M.
- The Journal of Chemical Physics, Vol. 134, Issue 8
Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser
journal, June 2018
- Nogly, Przemyslaw; Weinert, Tobias; James, Daniel
- Science
Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein
journal, May 2016
- Pande, K.; Hutchison, C. D. M.; Groenhof, G.
- Science, Vol. 352, Issue 6286
Generalization of the Mulliken-Hush treatment for the calculation of electron transfer matrix elements
journal, January 1996
- Cave, Robert J.; Newton, Marshall D.
- Chemical Physics Letters, Vol. 249, Issue 1-2
Protein Catalysis of the Retinal Subpicosecond Photoisomerization in the Primary Process of Bacteriorhodopsin Photosynthesis
journal, August 1993
- Song, L.; El-Sayed, M. A.; Lanyi, J. K.
- Science, Vol. 261, Issue 5123
Functions of a New Photoreceptor Membrane
journal, October 1973
- Oesterhelt, D.; Stoeckenius, W.
- Proceedings of the National Academy of Sciences, Vol. 70, Issue 10
Following Photoinduced Dynamics in Bacteriorhodopsin with 7-fs Impulsive Vibrational Spectroscopy
journal, January 2007
- Kahan, Anat; Nahmias, Omer; Friedman, Noga
- Journal of the American Chemical Society, Vol. 129, Issue 3
Intruder state avoidance multireference Møller-Plesset perturbation theory: Multireference Møller-Plesset Perturbation Theory
journal, May 2002
- Witek, Henryk A.; Choe, Yoong-Kee; Finley, James P.
- Journal of Computational Chemistry, Vol. 23, Issue 10
Theory and Simulation of the Ultrafast Double-Bond Isomerization of Biological Chromophores
journal, October 2017
- Gozem, Samer; Luk, Hoi Ling; Schapiro, Igor
- Chemical Reviews, Vol. 117, Issue 22
Measurements of the driving forces of bio-motors using the fluctuation theorem
journal, January 2012
- Hayashi, Kumiko; Tanigawara, Mizue; Kishikawa, Jun-ichi
- BIOPHYSICS, Vol. 8
Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography
journal, August 2014
- Liu, Wei; Ishchenko, Andrii; Cherezov, Vadim
- Nature Protocols, Vol. 9, Issue 9
Light-dark adaptation of bacteriorhodopsin in Triton-treated purple membrane
journal, March 1980
- Casadio, R.; Gutowitz, H.; Mowery, P.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 590, Issue 1
Spectral encoding of x-ray/optical relative delay
journal, January 2011
- Bionta, Mina R.; Lemke, H. T.; Cryan, J. P.
- Optics Express, Vol. 19, Issue 22
Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser
text, January 2018
- Nogly, Przemyslaw; Weinert, Tobias; James, Daniel
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Probing Ultrafast Photochemistry of Retinal Proteins in the Near-IR: Bacteriorhodopsin and Anabaena Sensory Rhodopsin vs Retinal Protonated Schiff Base in Solution
journal, November 2012
- Wand, Amir; Loevsky, Boris; Friedman, Noga
- The Journal of Physical Chemistry B, Vol. 117, Issue 16
Application of Singular Value Decomposition to the Analysis of Time-Resolved Macromolecular X-Ray Data
journal, March 2003
- Schmidt, Marius; Rajagopal, Sudarshan; Ren, Zhong
- Biophysical Journal, Vol. 84, Issue 3
Asymmetric Toggling of a Natural Photoswitch: Ultrafast Spectroscopy of Anabaena Sensory Rhodopsin
journal, December 2011
- Wand, Amir; Rozin, Rinat; Eliash, Tamar
- Journal of the American Chemical Society, Vol. 133, Issue 51
Direct observation of the coherent nuclear response after the absorption of a photon
text, January 2014
- Liebel, Matz; Schnedermann, Christoph; Bassolino, Giovanni
- arXiv
Vibrationally coherent photochemistry in the femtosecond primary event of vision
journal, October 1994
- Wang, Q.; Schoenlein, R.; Peteanu, L.
- Science, Vol. 266, Issue 5184
Femtosecond Infrared Spectroscopy of Bacteriorhodopsin Chromophore Isomerization
journal, August 2002
- Herbst, J.
- Science, Vol. 297, Issue 5582
Excited-state reaction dynamics of bacteriorhodopsin studied by femtosecond spectroscopy
journal, February 1988
- Dobler, J.; Zinth, W.; Kaiser, W.
- Chemical Physics Letters, Vol. 144, Issue 2
Microsecond Light-Induced Proton Transfer to Flavin in the Blue Light Sensor Plant Cryptochrome
journal, October 2009
- Langenbacher, Thomas; Immeln, Dominik; Dick, Bernhard
- Journal of the American Chemical Society, Vol. 131, Issue 40
Formation dynamics and nature of tryptophan's primary photoproduct in aqueous solution
journal, January 2010
- Léonard, J.; Sharma, D.; Szafarowicz, B.
- Physical Chemistry Chemical Physics, Vol. 12, Issue 48
Protein Catalysis of the Retinal Subpicosecond Photoisomerization in the Primary Process of Bacteriorhodopsin Photosynthesis
journal, August 1993
- Song, L.; El-Sayed, M. A.; Lanyi, J. K.
- Science, Vol. 261, Issue 5123
X-ray detectors at the Linac Coherent Light Source
journal, April 2015
- Blaj, Gabriel; Caragiulo, Pietro; Carini, Gabriella
- Journal of Synchrotron Radiation, Vol. 22, Issue 3
Following Photoinduced Dynamics in Bacteriorhodopsin with 7-fs Impulsive Vibrational Spectroscopy
journal, January 2007
- Kahan, Anat; Nahmias, Omer; Friedman, Noga
- Journal of the American Chemical Society, Vol. 129, Issue 3
Structure of a Protein Photocycle Intermediate by Millisecond Time-Resolved Crystallography
journal, March 1997
- Genick, U. K.
- Science, Vol. 275, Issue 5305
The primary photoreaction of channelrhodopsin-1: wavelength dependent photoreactions induced by ground-state heterogeneity
journal, July 2015
- Stensitzki, Till; Muders, Vera; Schlesinger, Ramona
- Frontiers in Molecular Biosciences, Vol. 2
Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography
journal, September 2017
- Coquelle, Nicolas; Sliwa, Michel; Woodhouse, Joyce
- Nature Chemistry, Vol. 10, Issue 1
Oxidation of tryptophan and N-methylindole by N3.cntdot., Br2.-, and (SCN)2.- radicals in light- and heavy-water solutions: a pulse radiolysis study
journal, May 1991
- Solar, Sonja; Getoff, Nikola; Surdhar, Parminder S.
- The Journal of Physical Chemistry, Vol. 95, Issue 9
Removal of methyl groups from retinal controls the activity of bacteriorhodopsin
journal, May 1983
- Gaertner, Wolfgang; Towner, Paul; Hopf, Henning
- Biochemistry, Vol. 22, Issue 11
Aborted double bicycle-pedal isomerization with hydrogen bond breaking is the primary event of bacteriorhodopsin proton pumping
journal, November 2010
- Altoe, P.; Cembran, A.; Olivucci, M.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 47
Density‐functional thermochemistry. III. The role of exact exchange
journal, April 1993
- Becke, Axel D.
- The Journal of Chemical Physics, Vol. 98, Issue 7, p. 5648-5652
Extended multi-configuration quasi-degenerate perturbation theory: The new approach to multi-state multi-reference perturbation theory
journal, June 2011
- Granovsky, Alexander A.
- The Journal of Chemical Physics, Vol. 134, Issue 21
Development of the Colle-Salvetti correlation-energy formula into a functional of the electron density
text, January 1988
- Robert, Parr,; Chengteh, Lee,; Weitao, Yang,
- The University of North Carolina at Chapel Hill University Libraries
First-Order Interacting Space Approach to Excited-State Molecular Interaction: Solvatochromic Shift of p -Coumaric Acid and Retinal Schiff Base
journal, May 2018
- Yanai, Kazuma; Ishimura, Kazuya; Nakayama, Akira
- Journal of Chemical Theory and Computation, Vol. 14, Issue 7
Generation, shaping, and characterization of intense femtosecond pulses tunable from 3 to 20 μm
journal, January 2000
- Kaindl, Robert A.; Wurm, Matthias; Reimann, Klaus
- Journal of the Optical Society of America B, Vol. 17, Issue 12, p. 2086-2094
Energy transfer from retinal to amino acids — a time-resolved study of the ultraviolet emission of bacteriorhodopsin
journal, October 1986
- Polland, H. J.; Franz, M. A.; Zinth, W.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 851, Issue 3
General atomic and molecular electronic structure system
journal, November 1993
- Schmidt, Michael W.; Baldridge, Kim K.; Boatz, Jerry A.
- Journal of Computational Chemistry, Vol. 14, Issue 11, p. 1347-1363
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation
journal, February 2021
- Tillu, Vikas A.; Rae, James; Gao, Ya
- Nature Communications, Vol. 12, Issue 1
Deazaflavin reductive photocatalysis involves excited semiquinone radicals
text, January 2020
- Graml, Andreas; Neveselý, Tomáš; Kutta, Roger Jan
- Universität Regensburg
Gaussian basis sets for use in correlated molecular calculations. V. Core‐valence basis sets for boron through neon
journal, September 1995
- Woon, David E.; Dunning, Thom H.
- The Journal of Chemical Physics, Vol. 103, Issue 11
Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein
dataset, January 2019
- Pande, K.
- Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); UWM, LBNL, SLAC, CFEL, U Chicago
Sub-nanosecond tryptophan radical deprotonation mediated by a protein-bound water cluster in class II DNA photolyases
journal, January 2018
- Müller, Pavel; Ignatz, Elisabeth; Kiontke, Stephan
- Chemical Science, Vol. 9, Issue 5
Functions of a New Photoreceptor Membrane
journal, October 1973
- Oesterhelt, D.; Stoeckenius, W.
- Proceedings of the National Academy of Sciences, Vol. 70, Issue 10
First-Order Interacting Space Approach to Excited-State Molecular Interaction: Solvatochromic Shift of p -Coumaric Acid and Retinal Schiff Base
journal, May 2018
- Yanai, Kazuma; Ishimura, Kazuya; Nakayama, Akira
- Journal of Chemical Theory and Computation, Vol. 14, Issue 7
Intruder state avoidance multireference Møller-Plesset perturbation theory: Multireference Møller-Plesset Perturbation Theory
journal, May 2002
- Witek, Henryk A.; Choe, Yoong-Kee; Finley, James P.
- Journal of Computational Chemistry, Vol. 23, Issue 10
Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
journal, March 2015
- Nogly, Przemyslaw; James, Daniel; Wang, Dingjie
- IUCrJ, Vol. 2, Issue 2, p. 168-176
Structure of a Protein Photocycle Intermediate by Millisecond Time-Resolved Crystallography
journal, March 1997
- Genick, U. K.
- Science, Vol. 275, Issue 5305
Theory and Simulation of the Ultrafast Double-Bond Isomerization of Biological Chromophores
journal, October 2017
- Gozem, Samer; Luk, Hoi Ling; Schapiro, Igor
- Chemical Reviews, Vol. 117, Issue 22
Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography
July 2017
- Coquelle, Nicolas
- Nature Chemistry
A three-dimensional movie of structural changes in bacteriorhodopsin
journal, December 2016
- Nango, Eriko; Royant, Antoine; Kubo, Minoru
- Science, Vol. 354, Issue 6319, p. 1552-1557
Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography
dataset, January 2017
- Coquelle, Coquelle
- Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); Institut Laue Langevin
Microsecond Light-Induced Proton Transfer to Flavin in the Blue Light Sensor Plant Cryptochrome
journal, October 2009
- Langenbacher, Thomas; Immeln, Dominik; Dick, Bernhard
- Journal of the American Chemical Society, Vol. 131, Issue 40
A three-dimensional movie of structural changes in bacteriorhodopsin
dataset, January 2016
- Nango, E.; Royant, A.; Kubo, M.
- Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); The University of Tokyo, RIKEN SPring-8 Center
Formation dynamics and nature of tryptophan's primary photoproduct in aqueous solution
journal, January 2010
- Léonard, J.; Sharma, D.; Szafarowicz, B.
- Physical Chemistry Chemical Physics, Vol. 12, Issue 48
Femtosecond Time-Resolved Stimulated Raman Reveals the Birth of Bacteriorhodopsin’s J and K Intermediates
journal, June 2009
- Shim, Sangdeok; Dasgupta, Jyotishman; Mathies, Richard A.
- Journal of the American Chemical Society, Vol. 131, Issue 22
Retinal isomer ratio in dark-adapted purple membrane and bacteriorhodopsin monomers
journal, January 1989
- Scherrer, P.; Mathew, M. K.; Sperling, W.
- Biochemistry, Vol. 28, Issue 2
Improved Estimation of Structure-Factor Difference Amplitudesfrom Poorly Accurate Data
journal, September 1997
- Ursby, T.; Bourgeois, D.
- Acta Crystallographica Section A Foundations of Crystallography, Vol. 53, Issue 5
Control of retinal isomerization in bacteriorhodopsin in the high-intensity regime
journal, June 2009
- Florean, A. C.; Cardoza, D.; White, J. L.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 27
Real-time spectroscopy of transition states in bacteriorhodopsin during retinal isomerization
journal, November 2001
- Kobayashi, Takayoshi; Saito, Takashi; Ohtani, Hiroyuki
- Nature, Vol. 414, Issue 6863
Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation
journal, September 2015
- Barends, T. R. M.; Foucar, L.; Ardevol, A.
- Science, Vol. 350, Issue 6259
Coherent control of the isomerization of retinal in bacteriorhodopsin in the high intensity regime
journal, February 2011
- Prokhorenko, Valentyn I.; Halpin, Alexei; Johnson, Philip J. M.
- The Journal of Chemical Physics, Vol. 134, Issue 8
Direct Observation of the Coherent Nuclear Response after the Absorption of a Photon
journal, June 2014
- Liebel, M.; Schnedermann, C.; Bassolino, G.
- Physical Review Letters, Vol. 112, Issue 23
A consistent and accurate ab initio parametrization of density functional dispersion correction (DFT-D) for the 94 elements H-Pu
journal, April 2010
- Grimme, Stefan; Antony, Jens; Ehrlich, Stephan
- The Journal of Chemical Physics, Vol. 132, Issue 15
Works referencing / citing this record:
A tool for visualizing protein motions in time-resolved crystallography
text, January 2020
- Cecilia, Wickstrand,; Gergely, Katona,; Takanori, Nakane,
- ETH Zurich
Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin in the multiphoton regime and biological relevance
journal, March 2020
- Miller, R. J. Dwayne; Paré-Labrosse, Olivier; Sarracini, Antoine
- Nature Communications, Vol. 11, Issue 1
Time-resolved serial femtosecond crystallography at the European XFEL
text, January 2019
- Pandey, Suraj; Bean, Richard; Sato, Tokushi
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy
journal, February 2020
- Woodhouse, Joyce; Nass Kovacs, Gabriela; Coquelle, Nicolas
- Nature Communications, Vol. 11, Issue 1
Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy
journal, February 2020
- Woodhouse, Joyce; Nass Kovacs, Gabriela; Coquelle, Nicolas
- Nature Communications, Vol. 11, Issue 1
Time-resolved serial femtosecond crystallography at the European XFEL
journal, November 2019
- Pandey, Suraj; Bean, Richard; Sato, Tokushi
- Nature Methods