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Title: Differences in resource use lead to coexistence of seed-transmitted microbial populations

Abstract

Seeds are involved in the vertical transmission of microorganisms in plants and act as reservoirs for the plant microbiome. They could serve as carriers of pathogens, making the study of microbial interactions on seeds important in the emergence of plant diseases. We studied the influence of biological disturbances caused by seed transmission of two phytopathogenic agents, Alternaria brassicicola Abra43 (Abra43) and Xanthomonas campestris pv. campestris 8004 (Xcc8004), on the structure and function of radish seed microbial assemblages, as well as the nutritional overlap between Xcc8004 and the seed microbiome, to find seed microbial residents capable of outcompeting this pathogen. According to taxonomic and functional inference performed on metagenomics reads, no shift in structure and function of the seed microbiome was observed following Abra43 and Xcc8004 transmission. This lack of impact derives from a limited overlap in nutritional resources between Xcc8004 and the major bacterial populations of radish seeds. However, two native seed-associated bacterial strains belonging to Stenotrophomonas rhizophila displayed a high overlap with Xcc8004 regarding the use of resources; they might therefore limit its transmission. The strategy we used may serve as a foundation for the selection of seed indigenous bacterial strains that could limit seed transmission of pathogens.

Authors:
 [1]; ORCiD logo [2];  [3];  [1];  [2];  [1];  [1];  [4];  [4]; ORCiD logo [2];  [1]
  1. French National Inst. for Agricultural Research (INRA), Baeucouze (France)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Austrian Inst. of Technology, Tulln (Austria)
  4. French National Inst. for Agricultural Research (INRA), Castanet-Tolosan (France)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1546502
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Torres-Cortes, G., Garcia, Benjamin J., Compant, S., Rezki, S., Jones, Piet C., Preveaux, A., Briand, M., Roulet, A., Bouchez, O., Jacobson, Daniel A., and Barret, Mattieu. Differences in resource use lead to coexistence of seed-transmitted microbial populations. United States: N. p., 2019. Web. doi:10.1038/s41598-019-42865-9.
Torres-Cortes, G., Garcia, Benjamin J., Compant, S., Rezki, S., Jones, Piet C., Preveaux, A., Briand, M., Roulet, A., Bouchez, O., Jacobson, Daniel A., & Barret, Mattieu. Differences in resource use lead to coexistence of seed-transmitted microbial populations. United States. doi:10.1038/s41598-019-42865-9.
Torres-Cortes, G., Garcia, Benjamin J., Compant, S., Rezki, S., Jones, Piet C., Preveaux, A., Briand, M., Roulet, A., Bouchez, O., Jacobson, Daniel A., and Barret, Mattieu. Tue . "Differences in resource use lead to coexistence of seed-transmitted microbial populations". United States. doi:10.1038/s41598-019-42865-9. https://www.osti.gov/servlets/purl/1546502.
@article{osti_1546502,
title = {Differences in resource use lead to coexistence of seed-transmitted microbial populations},
author = {Torres-Cortes, G. and Garcia, Benjamin J. and Compant, S. and Rezki, S. and Jones, Piet C. and Preveaux, A. and Briand, M. and Roulet, A. and Bouchez, O. and Jacobson, Daniel A. and Barret, Mattieu},
abstractNote = {Seeds are involved in the vertical transmission of microorganisms in plants and act as reservoirs for the plant microbiome. They could serve as carriers of pathogens, making the study of microbial interactions on seeds important in the emergence of plant diseases. We studied the influence of biological disturbances caused by seed transmission of two phytopathogenic agents, Alternaria brassicicola Abra43 (Abra43) and Xanthomonas campestris pv. campestris 8004 (Xcc8004), on the structure and function of radish seed microbial assemblages, as well as the nutritional overlap between Xcc8004 and the seed microbiome, to find seed microbial residents capable of outcompeting this pathogen. According to taxonomic and functional inference performed on metagenomics reads, no shift in structure and function of the seed microbiome was observed following Abra43 and Xcc8004 transmission. This lack of impact derives from a limited overlap in nutritional resources between Xcc8004 and the major bacterial populations of radish seeds. However, two native seed-associated bacterial strains belonging to Stenotrophomonas rhizophila displayed a high overlap with Xcc8004 regarding the use of resources; they might therefore limit its transmission. The strategy we used may serve as a foundation for the selection of seed indigenous bacterial strains that could limit seed transmission of pathogens.},
doi = {10.1038/s41598-019-42865-9},
journal = {Scientific Reports},
number = 1,
volume = 9,
place = {United States},
year = {2019},
month = {4}
}

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