LurR is a regulator of the central lactate oxidation pathway in sulfate-reducing Desulfovibrio species

Rajeev, Lara; Luning, Eric G.; Zane, Grant M.; Juba, Thomas R.; Kazakov, Alexey E.; Novichkov, Pavel S.; Wall, Judy D.; Mukhopadhyay, Aindrila; Wen, Z. Tom

doi:10.1371/journal.pone.0214960

Title: LurR is a regulator of the central lactate oxidation pathway in sulfate-reducing Desulfovibrio species

Journal Article · 09 April 2019 · PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0214960 · OSTI ID:1545315

Rajeev, Lara ^[1]; Luning, Eric G. ^[1]; Zane, Grant M. ^[2]; Juba, Thomas R. ^[2];

^[1]; Novichkov, Pavel S. ^[1]; Wall, Judy D. ^[2];

^[1]; Wen, Z. Tom ^[3]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of Missouri, Columbia, MO (United States)
LSU Health Sciences Center School of Dentistry, New Orleans, LA (United States)

The central carbon/lactate utilization pathway in the model sulfate-reducing bacterium, Desulfovibrio vulgaris Hildenborough, is encoded by the highly conserved operon DVU3025-3033. Our earlier in vitro genome-wide study had suggested a network of four two-component system regulators that target this large operon; however, how these four regulators control this operon was not known. Here, we probe the regulation of the lactate utilization operon with mutant strains and DNA-protein binding assays. We show that the LurR response regulator is required for optimal growth and complete lactate utilization, and that it activates the DVU3025-3033 lactate oxidation operon as well as DVU2451, a lactate permease gene, in the presence of lactate. We show by electrophoretic mobility shift assays that LurR binds to three sites in the upstream region of DVU3025, the first gene of the operon. NrfR, a response regulator that is activated under nitrite stress, and LurR share similar binding site motifs and bind the same sites upstream of DVU3025. The DVU3025 promoter also has a binding site motif (Pho box) that is bound by PhoB, a two-component response regulator activated under phosphate limitation. The lactate utilization operon, the regulator LurR, and LurR binding sites are conserved across the order Desulfovibrionales whereas possible modulation of the lactate utilization genes by additional regulators such as NrfR and PhoB appears to be limited to D. vulgaris.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1545315

Journal Information:: PLoS ONE, Vol. 14, Issue 4; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 3 works

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