Methanol Accelerates DMPC Flip-Flop and Transfer: A SANS Study on Lipid Dynamics

Nguyen, Michael H. L.; DiPasquale, Mitchell; Rickeard, Brett W.; Stanley, Christopher B.; Kelley, Elizabeth G.; Marquardt, Drew

doi:10.1016/j.bpj.2019.01.021

Title: Methanol Accelerates DMPC Flip-Flop and Transfer: A SANS Study on Lipid Dynamics

Abstract

Methanol is a common solubilizing agent used to study transmembrane proteins/peptides in biological and synthetic membranes. Using small angle neutron scattering and a strategic contrast-matching scheme, we show that methanol has a major impact on lipid dynamics. Under increasing methanol concentrations, isotopically distinct 1,2-dimyristoyl-sn-glycero-3-phosphocholine large unilamellar vesicle populations exhibit increased mixing. Specifically, 1,2-dimyristoyl-sn-glycero-3-phosphocholine transfer and flip-flop kinetics display linear and exponential rate enhancements, respectively. Ultimately, methanol is capable of influencing the structure-function relationship associated with bilayer composition (e.g., lipid asymmetry). Here, the use of methanol as a carrier solvent, despite better simulating some biological conditions (e.g., antimicrobial attack), can help misconstrue lipid scrambling as the action of proteins or peptides, when in actuality it is a combination of solvent and biological agent. As bilayer compositional stability is crucial to cell survival and protein reconstitution, these results highlight the importance of methanol, and solvents in general, in biomembrane and proteolipid studies.

Authors:: Nguyen, Michael H. L.; DiPasquale, Mitchell; Rickeard, Brett W.; Stanley, Christopher B.; Kelley, Elizabeth G.; Marquardt, Drew

Publication Date:: Fri Mar 01 00:00:00 EST 2019

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1603692

Alternate Identifier(s):: OSTI ID: 1545221

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Published Article

Journal Name:: Biophysical Journal

Additional Journal Information:: Journal Name: Biophysical Journal Journal Volume: 116 Journal Issue: 5; Journal ID: ISSN 0006-3495

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS

Citation Formats


                    Nguyen, Michael H. L., DiPasquale, Mitchell, Rickeard, Brett W., Stanley, Christopher B., Kelley, Elizabeth G., and Marquardt, Drew. Methanol Accelerates DMPC Flip-Flop and Transfer: A SANS Study on Lipid Dynamics.  United States: N. p., 2019. 
Web.  doi:10.1016/j.bpj.2019.01.021.

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                    Nguyen, Michael H. L., DiPasquale, Mitchell, Rickeard, Brett W., Stanley, Christopher B., Kelley, Elizabeth G., & Marquardt, Drew. Methanol Accelerates DMPC Flip-Flop and Transfer: A SANS Study on Lipid Dynamics.  United States.  https://doi.org/10.1016/j.bpj.2019.01.021

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                    Nguyen, Michael H. L., DiPasquale, Mitchell, Rickeard, Brett W., Stanley, Christopher B., Kelley, Elizabeth G., and Marquardt, Drew. Fri .  
"Methanol Accelerates DMPC Flip-Flop and Transfer: A SANS Study on Lipid Dynamics".  United States.  https://doi.org/10.1016/j.bpj.2019.01.021.

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@article{osti_1603692,

  title        = {Methanol Accelerates DMPC Flip-Flop and Transfer: A SANS Study on Lipid Dynamics},

  author       = {Nguyen, Michael H. L. and DiPasquale, Mitchell and Rickeard, Brett W. and Stanley, Christopher B. and Kelley, Elizabeth G. and Marquardt, Drew},

  abstractNote = {Methanol is a common solubilizing agent used to study transmembrane proteins/peptides in biological and synthetic membranes. Using small angle neutron scattering and a strategic contrast-matching scheme, we show that methanol has a major impact on lipid dynamics. Under increasing methanol concentrations, isotopically distinct 1,2-dimyristoyl-sn-glycero-3-phosphocholine large unilamellar vesicle populations exhibit increased mixing. Specifically, 1,2-dimyristoyl-sn-glycero-3-phosphocholine transfer and flip-flop kinetics display linear and exponential rate enhancements, respectively. Ultimately, methanol is capable of influencing the structure-function relationship associated with bilayer composition (e.g., lipid asymmetry). Here, the use of methanol as a carrier solvent, despite better simulating some biological conditions (e.g., antimicrobial attack), can help misconstrue lipid scrambling as the action of proteins or peptides, when in actuality it is a combination of solvent and biological agent. As bilayer compositional stability is crucial to cell survival and protein reconstitution, these results highlight the importance of methanol, and solvents in general, in biomembrane and proteolipid studies.},

  doi          = {10.1016/j.bpj.2019.01.021},

  journal      = {Biophysical Journal},

  number       = 5,

  volume       = 116,

  place        = {United States},

  year         = {Fri Mar 01 00:00:00 EST 2019},

  month        = {Fri Mar 01 00:00:00 EST 2019}

}

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Transverse lipid organization dictates bending fluctuations in model plasma membranes
journal, January 2020

Rickeard, Brett W.; Nguyen, Michael H. L.; DiPasquale, Mitchell
Nanoscale, Vol. 12, Issue 3
DOI: 10.1039/c9nr07977g

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Title: Methanol Accelerates DMPC Flip-Flop and Transfer: A SANS Study on Lipid Dynamics

Abstract

Citation Formats

Transverse lipid organization dictates bending fluctuations in model plasma membranes journal, January 2020

Transverse lipid organization dictates bending fluctuations in model plasma membranes
journal, January 2020