Title: Cellulolytic enzyme-aided extraction of hemicellulose from switchgrass and its characteristics

Abstract

The isolation of biomass components with their intact structure is integral for understanding the characteristics of biomass. Compared to other major biomass components such as cellulose and lignin, hemicellulose remains a challenging component to be isolated from the plant cell wall without significant depolymerization and modification. Here, a novel cellulolytic enzyme-aided hemicellulose (CEH) isolation method was developed to isolate hemicellulose with a near-native branched structure from switchgrass. The structural characteristics of CEH were investigated and compared with hemicelluloses isolated by conventional alkaline extracted hemicellulose (AEH) and DMSO extracted hemicellulose (DMSOH) methods. Gel permeation chromatography (GPC) analysis indicated that CEH had a weight-average molecular weight of 44 kDa, which was comparable to that of AEH (43 kDa) but higher than that of DMSOH (37 kDa). The chemical composition analysis revealed that CEH retained a higher proportion of glucuronic acid compared to AEH and DMSOH. The 2D ¹³C– ¹H heteronuclear single quantum coherence (HSQC) NMR spectra containing the β-(1,4)-linked-D-xylan backbone, non-reducing-end peaks and both α- and β-reducing-end peaks in CEH were comparable to the spectra of the commercial beechwood xylan. CEH showed a highly branched hemicellulose structure, which retained methoxyl groups, O-acetyl groups, and 4- O-methyl-glucuronic acid attached to the xylan backbone.

Authors:

Ding, Jinhua  ^[1];

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Yoo, Chang Geun  ^[2];

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Pu, Yunqiao  ^[3];

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Meng, Xianzhi  ^[4];

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Bhagia, Samarthya  ^[5];

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• Search DOE PAGES for ORCID "0000-0002-9495-1880"
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Yu, Chongwen ^[6]; Ragauskas, Arthur J.  ^[5]

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• Search DOE PAGES for ORCID "0000-0002-3536-554X"
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+ Show Author Affiliations

1. Donghua Univ., Shanghai (China); Univ. of Tennessee, Knoxville, TN (United States)
2. State Univ. of New York (SUNY), Syracuse, NY (United States)
3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
4. Univ. of Tennessee, Knoxville, TN (United States)
5. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
6. Donghua Univ., Shanghai (China); Key Lab. of Textile Science & Technology (China)

Publication Date:

2019-06-13

Research Org.:

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

OSTI Identifier:

1545200

Alternate Identifier(s):

OSTI ID: 1529646

Grant/Contract Number:  

[AC05-00OR22725]

Resource Type:

Accepted Manuscript

Journal Name:

Green Chemistry

Additional Journal Information:

[ Journal Volume: 21; Journal Issue: 14]; Journal ID: ISSN 1463-9262

Publisher:

Royal Society of Chemistry

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; Hemicellulose; Cellulase; Structural properties; Glucuronic acid; Glucuronoarabinoxylan