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Title: Cellulolytic enzyme-aided extraction of hemicellulose from switchgrass and its characteristics

Abstract

The isolation of biomass components with their intact structure is integral for understanding the characteristics of biomass. Compared to other major biomass components such as cellulose and lignin, hemicellulose remains a challenging component to be isolated from the plant cell wall without significant depolymerization and modification. Here, a novel cellulolytic enzyme-aided hemicellulose (CEH) isolation method was developed to isolate hemicellulose with a near-native branched structure from switchgrass. The structural characteristics of CEH were investigated and compared with hemicelluloses isolated by conventional alkaline extracted hemicellulose (AEH) and DMSO extracted hemicellulose (DMSOH) methods. Gel permeation chromatography (GPC) analysis indicated that CEH had a weight-average molecular weight of 44 kDa, which was comparable to that of AEH (43 kDa) but higher than that of DMSOH (37 kDa). The chemical composition analysis revealed that CEH retained a higher proportion of glucuronic acid compared to AEH and DMSOH. The 2D 13C– 1H heteronuclear single quantum coherence (HSQC) NMR spectra containing the β-(1,4)-linked-D-xylan backbone, non-reducing-end peaks and both α- and β-reducing-end peaks in CEH were comparable to the spectra of the commercial beechwood xylan. CEH showed a highly branched hemicellulose structure, which retained methoxyl groups, O-acetyl groups, and 4- O-methyl-glucuronic acid attached to the xylan backbone.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [5];  [6]; ORCiD logo [5]
  1. Donghua Univ., Shanghai (China); Univ. of Tennessee, Knoxville, TN (United States)
  2. State Univ. of New York (SUNY), Syracuse, NY (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Univ. of Tennessee, Knoxville, TN (United States)
  5. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  6. Donghua Univ., Shanghai (China); Key Lab. of Textile Science & Technology (China)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1545200
Alternate Identifier(s):
OSTI ID: 1529646
Grant/Contract Number:  
[AC05-00OR22725]
Resource Type:
Accepted Manuscript
Journal Name:
Green Chemistry
Additional Journal Information:
[ Journal Volume: 21; Journal Issue: 14]; Journal ID: ISSN 1463-9262
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Hemicellulose; Cellulase; Structural properties; Glucuronic acid; Glucuronoarabinoxylan

Citation Formats

Ding, Jinhua, Yoo, Chang Geun, Pu, Yunqiao, Meng, Xianzhi, Bhagia, Samarthya, Yu, Chongwen, and Ragauskas, Arthur J. Cellulolytic enzyme-aided extraction of hemicellulose from switchgrass and its characteristics. United States: N. p., 2019. Web. doi:10.1039/c9gc00252a.
Ding, Jinhua, Yoo, Chang Geun, Pu, Yunqiao, Meng, Xianzhi, Bhagia, Samarthya, Yu, Chongwen, & Ragauskas, Arthur J. Cellulolytic enzyme-aided extraction of hemicellulose from switchgrass and its characteristics. United States. doi:10.1039/c9gc00252a.
Ding, Jinhua, Yoo, Chang Geun, Pu, Yunqiao, Meng, Xianzhi, Bhagia, Samarthya, Yu, Chongwen, and Ragauskas, Arthur J. Thu . "Cellulolytic enzyme-aided extraction of hemicellulose from switchgrass and its characteristics". United States. doi:10.1039/c9gc00252a.
@article{osti_1545200,
title = {Cellulolytic enzyme-aided extraction of hemicellulose from switchgrass and its characteristics},
author = {Ding, Jinhua and Yoo, Chang Geun and Pu, Yunqiao and Meng, Xianzhi and Bhagia, Samarthya and Yu, Chongwen and Ragauskas, Arthur J.},
abstractNote = {The isolation of biomass components with their intact structure is integral for understanding the characteristics of biomass. Compared to other major biomass components such as cellulose and lignin, hemicellulose remains a challenging component to be isolated from the plant cell wall without significant depolymerization and modification. Here, a novel cellulolytic enzyme-aided hemicellulose (CEH) isolation method was developed to isolate hemicellulose with a near-native branched structure from switchgrass. The structural characteristics of CEH were investigated and compared with hemicelluloses isolated by conventional alkaline extracted hemicellulose (AEH) and DMSO extracted hemicellulose (DMSOH) methods. Gel permeation chromatography (GPC) analysis indicated that CEH had a weight-average molecular weight of 44 kDa, which was comparable to that of AEH (43 kDa) but higher than that of DMSOH (37 kDa). The chemical composition analysis revealed that CEH retained a higher proportion of glucuronic acid compared to AEH and DMSOH. The 2D 13C–1H heteronuclear single quantum coherence (HSQC) NMR spectra containing the β-(1,4)-linked-D-xylan backbone, non-reducing-end peaks and both α- and β-reducing-end peaks in CEH were comparable to the spectra of the commercial beechwood xylan. CEH showed a highly branched hemicellulose structure, which retained methoxyl groups, O-acetyl groups, and 4-O-methyl-glucuronic acid attached to the xylan backbone.},
doi = {10.1039/c9gc00252a},
journal = {Green Chemistry},
number = [14],
volume = [21],
place = {United States},
year = {2019},
month = {6}
}

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Works referenced in this record:

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Cellulose microfibril angles and cell-wall polymers in different wood types of Pinus radiata
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Isolation of hemicelluloses from sugarcane bagasse at different temperatures: Structure and properties
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journal, March 2006

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Two dimensional NMR spectroscopy for molecular characterization of soil organic matter: Application to boreal soils and litter
journal, November 2017


    Works referencing / citing this record:

    In-situ annotation of carbohydrate diversity, abundance, and degradability in highly complex mixtures using NMR spectroscopy
    journal, October 2014


    Chemical characterisation of the whole plant cell wall of archaeological wood: an integrated approach
    journal, May 2017

    • Zoia, Luca; Tamburini, Diego; Orlandi, Marco
    • Analytical and Bioanalytical Chemistry, Vol. 409, Issue 17
    • DOI: 10.1007/s00216-017-0378-7

    Cellulose microfibril angles and cell-wall polymers in different wood types of Pinus radiata
    journal, April 2012


    Fractionation of Corn Fiber Treated by Soaking in Aqueous Ammonia (SAA) for Isolation of Hemicellulose B and Production of C5 Sugars by Enzyme Hydrolysis
    journal, March 2011

    • Nghiem, Nhuan P.; Montanti, Justin; Johnston, David B.
    • Applied Biochemistry and Biotechnology, Vol. 164, Issue 8
    • DOI: 10.1007/s12010-011-9220-4

    Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d 6
    journal, March 2008


    Enzymatic Saccharification of Lignocelluloses Should be Conducted at Elevated pH 5.2–6.2
    journal, October 2012


    Efficient extraction of xylan from delignified corn stover using dimethyl sulfoxide
    journal, September 2013


    Liquefaction of hydrothermally pretreated wheat straw at high-solids content by purified Trichoderma enzymes
    journal, January 2011


    Comparison of laboratory delignification methods, their selectivity, and impacts on physiochemical characteristics of cellulosic biomass
    journal, February 2013


    Pyrolysis mechanism study of minimally damaged hemicellulose polymers isolated from agricultural waste straw samples
    journal, August 2015


    Hemicellulose characterization of deuterated switchgrass
    journal, December 2018


    Fractional purification and bioconversion of hemicelluloses
    journal, July 2012


    Genetic modification of plant cell walls to enhance biomass yield and biofuel production in bioenergy crops
    journal, September 2016


    Fractional extraction and structural characterization of sugarcane bagasse hemicelluloses
    journal, June 2004


    Extraction and characterization of hemicelluloses from flax shives by different methods
    journal, January 2010


    Isolation and structural characterization of hemicelluloses from the bamboo species Phyllostachys incarnata Wen
    journal, August 2011


    Isolation of hemicelluloses from sugarcane bagasse at different temperatures: Structure and properties
    journal, April 2012


    Enzyme-aided alkaline extraction of oligosaccharides and polymeric xylan from hardwood kraft pulp
    journal, March 2013


    Fractionation and structural characterization of LiCl–DMSO soluble hemicelluloses from tomato
    journal, April 2013


    Isolation and characterization of acetylated glucuronoarabinoxylan from sugarcane bagasse and straw
    journal, January 2017


    Two dimensional NMR spectroscopy for molecular characterization of soil organic matter: Application to boreal soils and litter
    journal, November 2017


    Controlled precipitation and purification of hemicellulose from DMSO and DMSO/water mixtures by carbon dioxide as anti-solvent
    journal, June 2010

    • Haimer, Emmerich; Wendland, Martin; Potthast, Antje
    • The Journal of Supercritical Fluids, Vol. 53, Issue 1-3
    • DOI: 10.1016/j.supflu.2010.02.009

    Carbohydrate Structural Determination by NMR Spectroscopy:  Modern Methods and Limitations
    journal, December 2000

    • Duus, Jens Ø.; Gotfredsen, Charlotte H.; Bock, Klaus
    • Chemical Reviews, Vol. 100, Issue 12
    • DOI: 10.1021/cr990302n

    Hydrothermal Pretreatment of Switchgrass
    journal, April 2011

    • Hu, Zhoujian; Ragauskas, Arthur J.
    • Industrial & Engineering Chemistry Research, Vol. 50, Issue 8
    • DOI: 10.1021/ie101886d

    Hemicelluloses of Ragi (Finger Millet, Eleusine coracana , Indaf-15):  Isolation and Purification of an Alkali-Extractable Arabinoxylan from Native and Malted Hemicellulose B
    journal, March 2006

    • Subba Rao, M. V. S. S. T.; Muralikrishna, G.
    • Journal of Agricultural and Food Chemistry, Vol. 54, Issue 6
    • DOI: 10.1021/jf058144q

    Fractional Isolation and Chemical Structure of Hemicellulosic Polymers Obtained from Bambusa rigida Species
    journal, November 2010

    • Wen, Jia-Long; Sun, Yong-Chang; Xu, Feng
    • Journal of Agricultural and Food Chemistry, Vol. 58, Issue 21
    • DOI: 10.1021/jf1032153

    Investigating plant cell wall components that affect biomass recalcitrance in poplar and switchgrass
    journal, January 2013

    • DeMartini, Jaclyn D.; Pattathil, Sivakumar; Miller, Jeffrey S.
    • Energy & Environmental Science, Vol. 6, Issue 3
    • DOI: 10.1039/c3ee23801f

    Xylan Biosynthesis: News from the Grass
    journal, April 2010


    Lignin Composition and Structure in Young versus Adult Eucalyptus globulus Plants
    journal, November 2010

    • Rencoret, Jorge; Gutiérrez, Ana; Nieto, Lidia
    • Plant Physiology, Vol. 155, Issue 2
    • DOI: 10.1104/pp.110.167254

    Hemicelluloses
    journal, June 2010


    Acetylation of woody lignocellulose: significance and regulation
    journal, January 2013

    • Pawar, Prashant Mohan-Anupama; Koutaniemi, Sanna; Tenkanen, Maija
    • Frontiers in Plant Science, Vol. 4
    • DOI: 10.3389/fpls.2013.00118