Cellulolytic enzyme-aided extraction of hemicellulose from switchgrass and its characteristics

Ding, Jinhua; Yoo, Chang Geun; Pu, Yunqiao; Meng, Xianzhi; Bhagia, Samarthya; Yu, Chongwen; Ragauskas, Arthur J.

doi:10.1039/c9gc00252a

Title: Cellulolytic enzyme-aided extraction of hemicellulose from switchgrass and its characteristics

Journal Article · 12 June 2019 · Green Chemistry

DOI: https://doi.org/10.1039/c9gc00252a · OSTI ID:1545200

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Donghua Univ., Shanghai (China); Univ. of Tennessee, Knoxville, TN (United States)
State Univ. of New York (SUNY), Syracuse, NY (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Donghua Univ., Shanghai (China); Key Lab. of Textile Science & Technology (China)

The isolation of biomass components with their intact structure is integral for understanding the characteristics of biomass. Compared to other major biomass components such as cellulose and lignin, hemicellulose remains a challenging component to be isolated from the plant cell wall without significant depolymerization and modification. Here, a novel cellulolytic enzyme-aided hemicellulose (CEH) isolation method was developed to isolate hemicellulose with a near-native branched structure from switchgrass. The structural characteristics of CEH were investigated and compared with hemicelluloses isolated by conventional alkaline extracted hemicellulose (AEH) and DMSO extracted hemicellulose (DMSOH) methods. Gel permeation chromatography (GPC) analysis indicated that CEH had a weight-average molecular weight of 44 kDa, which was comparable to that of AEH (43 kDa) but higher than that of DMSOH (37 kDa). The chemical composition analysis revealed that CEH retained a higher proportion of glucuronic acid compared to AEH and DMSOH. The 2D ¹³C–¹H heteronuclear single quantum coherence (HSQC) NMR spectra containing the β-(1,4)-linked-D-xylan backbone, non-reducing-end peaks and both α- and β-reducing-end peaks in CEH were comparable to the spectra of the commercial beechwood xylan. CEH showed a highly branched hemicellulose structure, which retained methoxyl groups, O-acetyl groups, and 4-O-methyl-glucuronic acid attached to the xylan backbone.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1545200

Alternate ID(s):: OSTI ID: 1529646

Journal Information:: Green Chemistry, Journal Name: Green Chemistry Journal Issue: 14 Vol. 21; ISSN GRCHFJ; ISSN 1463-9262

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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