Association mapping, transcriptomics, and transient expression identify candidate genes mediating plant–pathogen interactions in a tree

Muchero, Wellington; Sondreli, Kelsey L.; Chen, Jin-Gui; Urbanowicz, Breeanna R.; Zhang, Jin; Singan, Vasanth; Yang, Yongil; Brueggeman, Robert S.; Franco-Coronado, Juan; Abraham, Nivi; Yang, Jeong-Yeh; Moremen, Kelley W.; Weisberg, Alexandra J.; Chang, Jeff H.; Lindquist, Erika; Barry, Kerrie; Ranjan, Priya; Jawdy, Sara; Schmutz, Jeremy; Tuskan, Gerald A.; LeBoldus, Jared M.

doi:10.1073/pnas.1804428115

Title: Association mapping, transcriptomics, and transient expression identify candidate genes mediating plant–pathogen interactions in a tree

Journal Article · 17 October 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.1804428115 · OSTI ID:1545198

^[1]; Sondreli, Kelsey L. ^[2];

^[1]; Urbanowicz, Breeanna R. ^[3];

^[1]; Singan, Vasanth ^[4];

^[1]; Brueggeman, Robert S. ^[5]; Franco-Coronado, Juan ^[5]; Abraham, Nivi ^[5]; Yang, Jeong-Yeh ^[3];

^[3]; Weisberg, Alexandra J. ^[2];

^[2]; Lindquist, Erika ^[4]; Barry, Kerrie ^[4];

^[1];

^[1]; Schmutz, Jeremy ^[6];

^[7] more »

Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Oregon State University, Corvallis, OR (United States)
University of Georgia, Athens, GA (United States)
US Department of Energy, Walnut Creek, CA (United States)
North Dakota State University, Fargo, ND (United States)
US Department of Energy, Walnut Creek, CA (United States); HudsonAlpha Institute for Biotechnology, Huntsville, AL (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); US Department of Energy, Walnut Creek, CA (United States)
Oregon State University, Corvallis, OR (United States); North Dakota State University, Fargo, ND (United States)

Invasive microbes causing diseases such as sudden oak death negatively affect ecosystems and economies around the world. The deployment of resistant genotypes for combating introduced diseases typically relies on breeding programs that can take decades to complete. To reflect how this process can be accelerated, we employed a genome-wide association mapping of ca. 1,000 resequenced Populus trichocarpa trees individually challenged with Sphaerulina musiva, an invasive fungal pathogen. Among crucial associations, three loci associated with resistance were identified and predicted to encode one putative membrane-bound L-type receptor-like kinase and two receptor-like proteins. A susceptibility-associated locus was predicted to encode a putative G-type D-mannose–binding receptor-like kinase. Multiple lines of evidence, including allele analysis, transcriptomics, binding assays, and overexpression, support the hypothesized function of these candidate genes in the P. trichocarpa response to S. musiva.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: National Institutes of Health (NIH); US Department of Agriculture; USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; AC05-00OR22725; FG02-93ER20097; SC0015662; SC0018196

OSTI ID:: 1545198

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 45 Vol. 115; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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The Road to Resistance in Forest Trees Naidoo, Sanushka; Slippers, Bernard; Plett, Jonathan M. Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.00273	journal	March 2019

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Title: Association mapping, transcriptomics, and transient expression identify candidate genes mediating plant–pathogen interactions in a tree

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