Title: UV Laser-Induced, Time-Resolved Transcriptome Responses of Saccharomyces cerevisiae

Abstract

We observed the effect on gene transcription of laser-mediated, long-wavelength UV-irradiation of Saccharomyces cerevisiae by RNAseq analysis at times T15, T30, and T60 minutes after recovery in growth medium. Laser-irradiated cells were viable, and the transcriptional response was transient, with over 400 genes differentially expressed at T15 or T30, returning to basal level transcription by T60. Identification of transcripts exhibiting enhanced differential expression that were unique to UV laser-irradiation were identified by imposing a stringent significance cut-off (p <0.05, log₂ difference >2) then filtering out genes known as environmental stress response (ESR) genes. Using these rigorous criteria, 56 genes were differentially expressed at T15; at T30 differential expression was observed for 57 genes, some of which persisted from T15. Among the highly up-regulated genes were those supporting amino acid metabolic processes sulfur amino acids, methionine, aspartate, cysteine, serine), sulfur regulation (hydrogen sulfite metabolic processes, sulfate assimilation, sulfate reduction), proteasome components, amino acid transporters, and the iron regulon. At T30, the expression profile shifted to expression of transcripts related to catabolic processes (oxidoreductase activity, peptidase activity). Transcripts common to both T15 and T30 suggested an up-regulation of catabolic events, including UV damage response genes, and protein catabolism via proteasome and peptidasemore » activity. Specific genes encoding tRNAs were among the down-regulated genes adding to the suggestion that control of protein biosynthesis was a major response to long-wave UV laser irradiation. These transcriptional responses bring light to the remarkable ability of the yeast cell to respond to a UV-induced environmental insult.« less

Authors:

Hauser, Melinda ^[1]; Abraham, Paul E.  ^[2];

• Search DOE PAGES for author "Abraham, Paul E."
• Search DOE PAGES for ORCID "0000-0003-2685-9123"

Barcelona, Lorenz ^[1]; Becker, Jeffrey M.  ^[1]

• Search DOE PAGES for author "Becker, Jeffrey M."
• Search DOE PAGES for ORCID "0000-0003-0467-5913"

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+ Show Author Affiliations

1. Univ. of Tennessee, Knoxville, TN (United States)
2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Publication Date:

Tue Jun 18 00:00:00 EDT 2019

Research Org.:

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1545186

Grant/Contract Number:  

AC05-00OR22725

Resource Type:

Accepted Manuscript

Journal Name:

G3

Additional Journal Information:

Journal Volume: 9; Journal Issue: 7; Journal ID: ISSN 2160-1836

Publisher:

Genetics Society of America

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES