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Title: UV Laser-Induced, Time-Resolved Transcriptome Responses of Saccharomyces cerevisiae

Abstract

We observed the effect on gene transcription of laser-mediated, long-wavelength UV-irradiation of Saccharomyces cerevisiae by RNAseq analysis at times T15, T30, and T60 minutes after recovery in growth medium. Laser-irradiated cells were viable, and the transcriptional response was transient, with over 400 genes differentially expressed at T15 or T30, returning to basal level transcription by T60. Identification of transcripts exhibiting enhanced differential expression that were unique to UV laser-irradiation were identified by imposing a stringent significance cut-off (p <0.05, log 2 difference >2) then filtering out genes known as environmental stress response (ESR) genes. Using these rigorous criteria, 56 genes were differentially expressed at T15; at T30 differential expression was observed for 57 genes, some of which persisted from T15. Among the highly up-regulated genes were those supporting amino acid metabolic processes sulfur amino acids, methionine, aspartate, cysteine, serine), sulfur regulation (hydrogen sulfite metabolic processes, sulfate assimilation, sulfate reduction), proteasome components, amino acid transporters, and the iron regulon. At T30, the expression profile shifted to expression of transcripts related to catabolic processes (oxidoreductase activity, peptidase activity). Transcripts common to both T15 and T30 suggested an up-regulation of catabolic events, including UV damage response genes, and protein catabolism via proteasome andmore » peptidase activity. Specific genes encoding tRNAs were among the down-regulated genes adding to the suggestion that control of protein biosynthesis was a major response to long-wave UV laser irradiation. These transcriptional responses bring light to the remarkable ability of the yeast cell to respond to a UV-induced environmental insult.« less

Authors:
 [1]; ORCiD logo [2];  [1]; ORCiD logo [1]
  1. Univ. of Tennessee, Knoxville, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1545186
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
G3
Additional Journal Information:
Journal Volume: 9; Journal Issue: 7; Journal ID: ISSN 2160-1836
Publisher:
Genetics Society of America
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Hauser, Melinda, Abraham, Paul E., Barcelona, Lorenz, and Becker, Jeffrey M. UV Laser-Induced, Time-Resolved Transcriptome Responses of Saccharomyces cerevisiae. United States: N. p., 2019. Web. doi:10.1534/g3.119.400291.
Hauser, Melinda, Abraham, Paul E., Barcelona, Lorenz, & Becker, Jeffrey M. UV Laser-Induced, Time-Resolved Transcriptome Responses of Saccharomyces cerevisiae. United States. doi:10.1534/g3.119.400291.
Hauser, Melinda, Abraham, Paul E., Barcelona, Lorenz, and Becker, Jeffrey M. Tue . "UV Laser-Induced, Time-Resolved Transcriptome Responses of Saccharomyces cerevisiae". United States. doi:10.1534/g3.119.400291.
@article{osti_1545186,
title = {UV Laser-Induced, Time-Resolved Transcriptome Responses of Saccharomyces cerevisiae},
author = {Hauser, Melinda and Abraham, Paul E. and Barcelona, Lorenz and Becker, Jeffrey M.},
abstractNote = {We observed the effect on gene transcription of laser-mediated, long-wavelength UV-irradiation of Saccharomyces cerevisiae by RNAseq analysis at times T15, T30, and T60 minutes after recovery in growth medium. Laser-irradiated cells were viable, and the transcriptional response was transient, with over 400 genes differentially expressed at T15 or T30, returning to basal level transcription by T60. Identification of transcripts exhibiting enhanced differential expression that were unique to UV laser-irradiation were identified by imposing a stringent significance cut-off (p <0.05, log2 difference >2) then filtering out genes known as environmental stress response (ESR) genes. Using these rigorous criteria, 56 genes were differentially expressed at T15; at T30 differential expression was observed for 57 genes, some of which persisted from T15. Among the highly up-regulated genes were those supporting amino acid metabolic processes sulfur amino acids, methionine, aspartate, cysteine, serine), sulfur regulation (hydrogen sulfite metabolic processes, sulfate assimilation, sulfate reduction), proteasome components, amino acid transporters, and the iron regulon. At T30, the expression profile shifted to expression of transcripts related to catabolic processes (oxidoreductase activity, peptidase activity). Transcripts common to both T15 and T30 suggested an up-regulation of catabolic events, including UV damage response genes, and protein catabolism via proteasome and peptidase activity. Specific genes encoding tRNAs were among the down-regulated genes adding to the suggestion that control of protein biosynthesis was a major response to long-wave UV laser irradiation. These transcriptional responses bring light to the remarkable ability of the yeast cell to respond to a UV-induced environmental insult.},
doi = {10.1534/g3.119.400291},
journal = {G3},
number = 7,
volume = 9,
place = {United States},
year = {2019},
month = {6}
}

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