A High‐Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity
Abstract
Abstract This article describes a screening platform to identify compounds that affect human embryonic vascular development. The procedure comprises the generation of human embryonic‐like endothelial cells (ECs) from human pluripotent stem cells (hPSCs) and subsequent maturation under arterial flow conditions; the use of these cells for the high‐throughput screening of small molecules that specifically inhibit the survival of embryonic‐like ECs; the confirmation of the hits in embryonic‐like ECs cultured under flow shear stress; and final validation in mouse embryonic ECs. The embryonic‐like ECs express embryonic genes including DLL1 , EPHB2 , LYN , TEK , ID1, NRP2, CAST, FLT1, IGF1, DKK3, NIN, LEF1 , and SORBS3 . The entire screening procedure (without the validation step) can be completed within 1 month. This platform is an alternative/complement to standard animal protocols for assessing the effects of chemicals on embryonic vascular development. © 2019 by John Wiley & Sons, Inc.
- Authors:
-
- CNC‐Center for Neurosciences and Cell Biology University of Coimbra Rua Larga Coimbra Portugal
- Faculty of Medicine University of Coimbra Health Sciences Campus, Pólo III, Central Unit Coimbra Portugal
- CNC‐Center for Neurosciences and Cell Biology University of Coimbra Rua Larga Coimbra Portugal, Faculty of Medicine University of Coimbra Health Sciences Campus, Pólo III, Central Unit Coimbra Portugal
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1545175
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Current Protocols in Stem Cell Biology
- Additional Journal Information:
- Journal Name: Current Protocols in Stem Cell Biology Journal Volume: 50 Journal Issue: 1; Journal ID: ISSN 1941-7322
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Citation Formats
Rosa, Susana, Pitrez, Patrícia, Fernandes, Hugo, and Ferreira, Lino. A High‐Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity. Country unknown/Code not available: N. p., 2019.
Web. doi:10.1002/cpsc.93.
Rosa, Susana, Pitrez, Patrícia, Fernandes, Hugo, & Ferreira, Lino. A High‐Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity. Country unknown/Code not available. https://doi.org/10.1002/cpsc.93
Rosa, Susana, Pitrez, Patrícia, Fernandes, Hugo, and Ferreira, Lino. Fri .
"A High‐Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity". Country unknown/Code not available. https://doi.org/10.1002/cpsc.93.
@article{osti_1545175,
title = {A High‐Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity},
author = {Rosa, Susana and Pitrez, Patrícia and Fernandes, Hugo and Ferreira, Lino},
abstractNote = {Abstract This article describes a screening platform to identify compounds that affect human embryonic vascular development. The procedure comprises the generation of human embryonic‐like endothelial cells (ECs) from human pluripotent stem cells (hPSCs) and subsequent maturation under arterial flow conditions; the use of these cells for the high‐throughput screening of small molecules that specifically inhibit the survival of embryonic‐like ECs; the confirmation of the hits in embryonic‐like ECs cultured under flow shear stress; and final validation in mouse embryonic ECs. The embryonic‐like ECs express embryonic genes including DLL1 , EPHB2 , LYN , TEK , ID1, NRP2, CAST, FLT1, IGF1, DKK3, NIN, LEF1 , and SORBS3 . The entire screening procedure (without the validation step) can be completed within 1 month. This platform is an alternative/complement to standard animal protocols for assessing the effects of chemicals on embryonic vascular development. © 2019 by John Wiley & Sons, Inc.},
doi = {10.1002/cpsc.93},
journal = {Current Protocols in Stem Cell Biology},
number = 1,
volume = 50,
place = {Country unknown/Code not available},
year = {Fri Jul 26 00:00:00 EDT 2019},
month = {Fri Jul 26 00:00:00 EDT 2019}
}
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