Protection of abasic sites during DNA replication by a stable thiazolidine protein-DNA cross-link

Thompson, Petria S.; Amidon, Katherine M.; Mohni, Kareem N.; Cortez, David; Eichman, Brandt F.

doi:10.1038/s41594-019-0255-5

Title: Protection of abasic sites during DNA replication by a stable thiazolidine protein-DNA cross-link

Journal Article · 24 June 2019 · Nature Structural & Molecular Biology

DOI: https://doi.org/10.1038/s41594-019-0255-5 · OSTI ID:1544876

Thompson, Petria S. ^[1];

^[2]; Mohni, Kareem N. ^[1];

^[1];

^[3]

Vanderbilt Univ. School of Medicine, Nashville, TN (United States)
Vanderbilt Univ., Nashville, TN (United States)
Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Vanderbilt Univ., Nashville, TN (United States)

Abasic (AP) sites are one of the most common DNA lesions that block replicative polymerases. 5-hydroxymethylcytosine binding, embryonic stem cell-specific protein (HMCES) recognizes and processes these lesions in the context of single-stranded DNA (ssDNA). A HMCES DNA-protein cross-link (DPC) intermediate is thought to shield the AP site from endonucleases and error-prone polymerases. The highly evolutionarily conserved SOS-response associated peptidase (SRAP) domain of HMCES and its Escherichia coli ortholog YedK mediate lesion recognition. Here we uncover the basis of AP site protection by SRAP domains from a crystal structure of the YedK DPC. We report YedK forms a stable thiazolidine linkage between a ring-opened AP site and the α-amino and sulfhydryl substituents of its amino-terminal cysteine residue. The thiazolidine linkage explains the remarkable stability of the HMCES DPC, its resistance to strand cleavage and the proteolysis requirement for resolution. Furthermore, its structure reveals that HMCES has specificity for AP sites in ssDNA at junctions found when replicative polymerases encounter the AP lesion.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); Vanderbilt Molecular Biophysics Training Program; Vanderbilt-Ingram Cancer Center; USDOE Office of Science (SC), Basic Energy Sciences (BES); Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor

Grant/Contract Number:: R01ES030575; R01GM117299; P01CA092584; AC02-06CH11357; 085P1000817

OSTI ID:: 1544876

Journal Information:: Nature Structural & Molecular Biology, Vol. 26, Issue 7; ISSN 1545-9993

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 59 works

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