Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria
Abstract
Photosynthesis uses solar energy to drive inorganic carbon (Ci) uptake, fixation, and biomass formation. In cyanobacteria, Ci uptake is assisted by carbon concentrating mechanisms (CCM), and CO2 fixation is catalyzed by RubisCO in the Calvin-Benson-Bassham (CBB) cycle. Understanding the regulation that governs CCM and CBB cycle activities in natural and engineered strains requires methods and parameters that quantify these activities. Here, we used membrane-inlet mass spectrometry (MIMS) to simultaneously quantify Ci concentrating and fixation processes in the cyanobacterium Synechocystis 6803. By comparing cultures acclimated to ambient air conditions to cultures transitioning to high Ci conditions, we show that acclimation to high Ci involves a concurrent decline of Ci uptake and fixation parameters. By varying light input, we show that both CCM and CBB reactions become energy limited under low light conditions. A strain over-expressing the gene for the CBB cycle enzyme fructose-bisphosphate aldolase showed higher CCM and carbon fixation capabilities, suggesting a regulatory link between CBB metabolites and CCM capacity. While the engineering of an ethanol production pathway had no effect on CCM or carbon fixation parameters, additional fructose-bisphosphate aldolase gene over-expression enhanced both activities while simultaneously increasing ethanol productivity. These observations show that MIMS can be a useful toolmore »
- Authors:
-
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Uppsala Univ., Uppsala (Sweden)
- Publication Date:
- Research Org.:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 1544524
- Report Number(s):
- NREL/JA-2700-72738
Journal ID: ISSN 1664-302X
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 10; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; CCU; MIMS; carbon uptake rate; cyanobacteria; FbaA
Citation Formats
Douchi, Damien, Liang, Feiyan, Cano, Melissa, Xiong, Wei, Wang, Bo, Maness, Pin -Ching, Lindblad, Peter, and Yu, Jianping. Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria. United States: N. p., 2019.
Web. doi:10.3389/fmicb.2019.01356.
Douchi, Damien, Liang, Feiyan, Cano, Melissa, Xiong, Wei, Wang, Bo, Maness, Pin -Ching, Lindblad, Peter, & Yu, Jianping. Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria. United States. doi:https://doi.org/10.3389/fmicb.2019.01356
Douchi, Damien, Liang, Feiyan, Cano, Melissa, Xiong, Wei, Wang, Bo, Maness, Pin -Ching, Lindblad, Peter, and Yu, Jianping. Tue .
"Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria". United States. doi:https://doi.org/10.3389/fmicb.2019.01356. https://www.osti.gov/servlets/purl/1544524.
@article{osti_1544524,
title = {Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria},
author = {Douchi, Damien and Liang, Feiyan and Cano, Melissa and Xiong, Wei and Wang, Bo and Maness, Pin -Ching and Lindblad, Peter and Yu, Jianping},
abstractNote = {Photosynthesis uses solar energy to drive inorganic carbon (Ci) uptake, fixation, and biomass formation. In cyanobacteria, Ci uptake is assisted by carbon concentrating mechanisms (CCM), and CO2 fixation is catalyzed by RubisCO in the Calvin-Benson-Bassham (CBB) cycle. Understanding the regulation that governs CCM and CBB cycle activities in natural and engineered strains requires methods and parameters that quantify these activities. Here, we used membrane-inlet mass spectrometry (MIMS) to simultaneously quantify Ci concentrating and fixation processes in the cyanobacterium Synechocystis 6803. By comparing cultures acclimated to ambient air conditions to cultures transitioning to high Ci conditions, we show that acclimation to high Ci involves a concurrent decline of Ci uptake and fixation parameters. By varying light input, we show that both CCM and CBB reactions become energy limited under low light conditions. A strain over-expressing the gene for the CBB cycle enzyme fructose-bisphosphate aldolase showed higher CCM and carbon fixation capabilities, suggesting a regulatory link between CBB metabolites and CCM capacity. While the engineering of an ethanol production pathway had no effect on CCM or carbon fixation parameters, additional fructose-bisphosphate aldolase gene over-expression enhanced both activities while simultaneously increasing ethanol productivity. These observations show that MIMS can be a useful tool to study the extracellular Ci flux and how CBB metabolites regulate Ci uptake and fixation.},
doi = {10.3389/fmicb.2019.01356},
journal = {Frontiers in Microbiology},
number = ,
volume = 10,
place = {United States},
year = {2019},
month = {6}
}
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Figures / Tables:
FIGURE 1: A schematic representation of the relationship between photosynthetically harvested energy by the photosynthetic electron transfer chain (PETC), inorganic carbon (Ci) uptake and fixation, and the anabolic metabolism. Orange arrows indicate chemical energy fluxes. Black arrows indicate carbon flux.
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Works referenced in this record:
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journal, October 2014
- Adebiyi, Adeola O.; Jazmin, Lara J.; Young, Jamey D.
- Photosynthesis Research, Vol. 126, Issue 1
Synthetic DNA system for structure-function studies of the high affinity CO2 uptake NDH-13 protein complex in cyanobacteria
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Cyanobacterial NDH-1 complexes: Novel insights and remaining puzzles
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Early Evolution of Photosynthesis
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Regulation of CO2 Concentrating Mechanism in Cyanobacteria
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Genome-wide analysis of the fructose 1,6-bisphosphate aldolase (FBA) gene family and functional characterization of FBA7 in tomato
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The kinetics of the reaction CO2 + H2O → H+ + HCO3− as one of the rate limiting steps for the dissolution of calcite in the system H2OCO2CaCO3
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Cellular inorganic carbon fluxes in Trichodesmium: a combined approach using measurements and modelling
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Simultaneous Transport of CO 2 and HCO 3 − by the Cyanobacterium Synechococcus UTEX 625
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The single-process biochemical reaction of Rubisco: A unified theory and model with the effects of irradiance, CO2 and rate-limiting step on the kinetics of C3 and C4 photosynthesis from gas exchange
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Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO 2
journal, January 2016
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- Energy & Environmental Science, Vol. 9, Issue 4
A moderate decrease of plastid aldolase activity inhibits photosynthesis, alters the levels of sugars and starch, and inhibits growth of potato plants: Decrease of plastid aldolase activity inhibits photosynthesis
journal, April 1998
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- The Plant Journal, Vol. 14, Issue 2
A Small Decrease of Plastid Transketolase Activity in Antisense Tobacco Transformants Has Dramatic Effects on Photosynthesis and Phenylpropanoid Metabolism
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- The Plant Cell, Vol. 13, Issue 3
A mass spectrometer inlet system for sampling gases dissolved in liquid phases
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Impacts of genetically engineered alterations in carbon sink pathways on photosynthetic performance
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Installing extra bicarbonate transporters in the cyanobacterium Synechocystis sp. PCC6803 enhances biomass production
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- Kamennaya, Nina A.; Ahn, SeEun; Park, Hanwool
- Metabolic Engineering, Vol. 29
A Small Decrease in Rubisco Content by Individual Suppression of RBCS Genes Leads to Improvement of Photosynthesis and Greater Biomass Production in Rice Under Conditions of Elevated CO2
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CO 2 CONCENTRATING MECHANISMS IN PHOTOSYNTHETIC MICROORGANISMS
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Combined Effects of CO 2 and Light on the N 2 -Fixing Cyanobacterium Trichodesmium IMS101: Physiological Responses
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- Plant Physiology, Vol. 154, Issue 1
Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio
journal, March 2018
- Liang, Feiyan; Englund, Elias; Lindberg, Pia
- Metabolic Engineering, Vol. 46
Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803
journal, November 2016
- Liang, Feiyan; Lindblad, Peter
- Metabolic Engineering, Vol. 38
Synechocystis PCC 6803 overexpressing RuBisCO grow faster with increased photosynthesis
journal, June 2017
- Liang, Feiyan; Lindblad, Peter
- Metabolic Engineering Communications, Vol. 4
Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories
journal, November 2015
- Luan, Guodong; Qi, Yunjing; Wang, Min
- Biotechnology for Biofuels, Vol. 8, Issue 1
Exogenous expression of the wheat chloroplastic fructose-1,6-bisphosphatase gene enhances photosynthesis in the transgenic cyanobacterium, Anabaena PCC7120
journal, May 2005
- Ma, Weimin; Shi, Dingji; Wang, Quanxi
- Journal of Applied Phycology, Vol. 17, Issue 3
Glycolaldehyde Inhibits CO 2 Fixation in the Cyanobacterium Synechococcus UTEX 625 without Inhibiting the Accumulation of Inorganic Carbon or the Associated Quenching of Chlorophyll a Fluorescence
journal, November 1989
- Miller, Anthony G.; Canvin, David T.
- Plant Physiology, Vol. 91, Issue 3
Active Transport of CO 2 by the Cyanobacterium Synechococcus UTEX 625 : Measurement by Mass Spectrometry
journal, March 1988
- Miller, Anthony G.; Espie, George S.; Canvin, David T.
- Plant Physiology, Vol. 86, Issue 3
Genome Engineering of the 2,3-Butanediol Biosynthetic Pathway for Tight Regulation in Cyanobacteria
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- ACS Synthetic Biology, Vol. 4, Issue 11
A modular system for the measurement of CO 2 and O 2 gas flux and photosynthetic electron transport in microalgae : Algal photosynthetic efficiency system
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The functioning of the CO 2 concentrating mechanism in several cyanobacterial strains: a review of general physiological characteristics, genes, proteins, and recent advances
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- Canadian Journal of Botany, Vol. 76, Issue 6
Photoreduction of O 2 Primes and Replaces CO 2 Assimilation
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- Plant Physiology, Vol. 58, Issue 3
Consistent Sets of Spectrophotometric Chlorophyll Equations for Acetone, Methanol and Ethanol Solvents
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Active uptake of CO2 during photosynthesis in the green alga Eremosphaera viridis is mediated by a CO2-ATPase
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Simultaneous stimulation of sedoheptulose 1,7-bisphosphatase, fructose 1,6-bisphophate aldolase and the photorespiratory glycine decarboxylase-H protein increases CO 2 assimilation, vegetative biomass and seed yield in Arabidopsis
journal, March 2017
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- Plant Biotechnology Journal, Vol. 15, Issue 7
Effects of carbon nutrition on the physiological expression of HCO 3 - transport and the CO 2 -concentrating mechanism in the cyanobacterium Chlorogloeopsis sp. ATCC 27193
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Catalytic exchange of 18 O from 13 C 18 O-labelled CO 2 by wild-type cells and ecaA , ecaB , and ccaA mutants of the cyanobacteria Synechococcus PCC7942 and Synechocystis PCC6803
journal, June 1998
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Characterisation of carbon dioxide and bicarbonate transport during steady-state photosynthesis in the marine cyanobacterium Synechococcus strain PCC7002
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A thylakoid‐located carbonic anhydrase regulates CO 2 uptake in the cyanobacterium Synechocystis sp. PCC 6803
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Passive Entry of CO 2 and Its Energy-dependent Intracellular Conversion to HCO in Cyanobacteria Are Driven by a Photosystem I-generated ΔμH +
journal, April 2001
- Tchernov, Dan; Helman, Yael; Keren, Nir
- Journal of Biological Chemistry, Vol. 276, Issue 26
Increased fructose 1,6-bisphosphate aldolase in plastids enhances growth and photosynthesis of tobacco plants
journal, February 2012
- Uematsu, Kimio; Suzuki, Nobuaki; Iwamae, Tomoko
- Journal of Experimental Botany, Vol. 63, Issue 8
Alterations in Global Patterns of Gene Expression in Synechocystis sp. PCC 6803 in Response to Inorganic Carbon Limitation and the Inactivation of ndhR , a LysR Family Regulator
journal, November 2003
- Wang, Hong-Liang; Postier, Bradley L.; Burnap, Robert L.
- Journal of Biological Chemistry, Vol. 279, Issue 7
Advancing Our Understanding and Capacity to Engineer Nature’s CO 2 -Sequestering Enzyme, Rubisco
journal, October 2010
- Whitney, Spencer M.; Houtz, Robert L.; Alonso, Hernan
- Plant Physiology, Vol. 155, Issue 1
Sensing of Inorganic Carbon Limitation in Synechococcus PCC7942 Is Correlated with the Size of the Internal Inorganic Carbon Pool and Involves Oxygen
journal, November 2005
- Woodger, Fiona J.; Badger, Murray R.; Price, G. Dean
- Plant Physiology, Vol. 139, Issue 4
Transcriptional Regulation of the CO2-Concentrating Mechanism in a Euryhaline, Coastal Marine Cyanobacterium, Synechococcus sp. Strain PCC 7002: Role of NdhR/CcmR
journal, February 2007
- Woodger, F. J.; Bryant, D. A.; Price, G. D.
- Journal of Bacteriology, Vol. 189, Issue 9
The plasticity of cyanobacterial carbon metabolism
journal, December 2017
- Xiong, Wei; Cano, Melissa; Wang, Bo
- Current Opinion in Chemical Biology, Vol. 41
The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene
journal, April 2015
- Xiong, Wei; Morgan, John A.; Ungerer, Justin
- Nature Plants, Vol. 1, Issue 5
Genetic engineering of the Calvin cycle toward enhanced photosynthetic CO2 fixation in microalgae
journal, October 2017
- Yang, Bo; Liu, Jin; Ma, Xiaonian
- Biotechnology for Biofuels, Vol. 10, Issue 1
Introducing extra NADPH consumption ability significantly increases the photosynthetic efficiency and biomass production of cyanobacteria
journal, November 2016
- Zhou, Jie; Zhang, Fuliang; Meng, Hengkai
- Metabolic Engineering, Vol. 38
Works referencing / citing this record:
Characterisation of carbon dioxide and bicarbonate transport during steady-state photosynthesis in the marine cyanobacterium Synechococcus strain PCC7002
journal, November 1995
- S�ltemeyer, Dieter; Price, G. Dean; Yu, Jian-Wei
- Planta, Vol. 197, Issue 4
Active uptake of CO2 during photosynthesis in the green alga Eremosphaera viridis is mediated by a CO2-ATPase
journal, January 1992
- Rotatore, Caterina; Lew, RogerR.; Colman, Brian
- Planta, Vol. 188, Issue 4
Effects of carbon nutrition on the physiological expression of HCO 3 - transport and the CO 2 -concentrating mechanism in the cyanobacterium Chlorogloeopsis sp. ATCC 27193
journal, February 2002
- Skleryk, Robert; So, Anthony; Espie, George
- Planta, Vol. 214, Issue 4
Exogenous expression of the wheat chloroplastic fructose-1,6-bisphosphatase gene enhances photosynthesis in the transgenic cyanobacterium, Anabaena PCC7120
journal, May 2005
- Ma, Weimin; Shi, Dingji; Wang, Quanxi
- Journal of Applied Phycology, Vol. 17, Issue 3
Consistent Sets of Spectrophotometric Chlorophyll Equations for Acetone, Methanol and Ethanol Solvents
journal, June 2006
- Ritchie, Raymond J.
- Photosynthesis Research, Vol. 89, Issue 1
13C flux analysis of cyanobacterial metabolism
journal, October 2014
- Adebiyi, Adeola O.; Jazmin, Lara J.; Young, Jamey D.
- Photosynthesis Research, Vol. 126, Issue 1
A mass spectrometer inlet system for sampling gases dissolved in liquid phases
journal, April 1963
- Hoch, George; Kok, Bessel
- Archives of Biochemistry and Biophysics, Vol. 101, Issue 1
The kinetics of the reaction CO2 + H2O → H+ + HCO3− as one of the rate limiting steps for the dissolution of calcite in the system H2OCO2CaCO3
journal, September 1996
- Dreybrodt, W.; Lauckner, J.; Zaihua, Liu
- Geochimica et Cosmochimica Acta, Vol. 60, Issue 18
Impacts of genetically engineered alterations in carbon sink pathways on photosynthetic performance
journal, December 2016
- Holland, Steven C.; Artier, Juliana; Miller, Neil T.
- Algal Research, Vol. 20
Cyanobacterial NDH-1 complexes: Novel insights and remaining puzzles
journal, August 2011
- Battchikova, Natalia; Eisenhut, Marion; Aro, Eva-Mari
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1807, Issue 8
Synthetic DNA system for structure-function studies of the high affinity CO2 uptake NDH-13 protein complex in cyanobacteria
journal, October 2018
- Artier, Juliana; Holland, Steven C.; Miller, Neil T.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1859, Issue 10
The single-process biochemical reaction of Rubisco: A unified theory and model with the effects of irradiance, CO2 and rate-limiting step on the kinetics of C3 and C4 photosynthesis from gas exchange
journal, February 2011
- Farazdaghi, Hadi
- Biosystems, Vol. 103, Issue 2
The plasticity of cyanobacterial carbon metabolism
journal, December 2017
- Xiong, Wei; Cano, Melissa; Wang, Bo
- Current Opinion in Chemical Biology, Vol. 41
Synechocystis PCC 6803 overexpressing RuBisCO grow faster with increased photosynthesis
journal, June 2017
- Liang, Feiyan; Lindblad, Peter
- Metabolic Engineering Communications, Vol. 4
Genome-wide analysis of the fructose 1,6-bisphosphate aldolase (FBA) gene family and functional characterization of FBA7 in tomato
journal, November 2016
- Cai, Bingbing; Li, Qiang; Xu, Yongchao
- Plant Physiology and Biochemistry, Vol. 108
Installing extra bicarbonate transporters in the cyanobacterium Synechocystis sp. PCC6803 enhances biomass production
journal, May 2015
- Kamennaya, Nina A.; Ahn, SeEun; Park, Hanwool
- Metabolic Engineering, Vol. 29
Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803
journal, November 2016
- Liang, Feiyan; Lindblad, Peter
- Metabolic Engineering, Vol. 38
Introducing extra NADPH consumption ability significantly increases the photosynthetic efficiency and biomass production of cyanobacteria
journal, November 2016
- Zhou, Jie; Zhang, Fuliang; Meng, Hengkai
- Metabolic Engineering, Vol. 38
Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio
journal, March 2018
- Liang, Feiyan; Englund, Elias; Lindberg, Pia
- Metabolic Engineering, Vol. 46
Genome Engineering of the 2,3-Butanediol Biosynthetic Pathway for Tight Regulation in Cyanobacteria
journal, May 2015
- Nozzi, Nicole E.; Atsumi, Shota
- ACS Synthetic Biology, Vol. 4, Issue 11
The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene
journal, April 2015
- Xiong, Wei; Morgan, John A.; Ungerer, Justin
- Nature Plants, Vol. 1, Issue 5
Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO 2
journal, January 2016
- Gao, Xiang; Gao, Fang; Liu, Deng
- Energy & Environmental Science, Vol. 9, Issue 4
A moderate decrease of plastid aldolase activity inhibits photosynthesis, alters the levels of sugars and starch, and inhibits growth of potato plants: Decrease of plastid aldolase activity inhibits photosynthesis
journal, April 1998
- Haake, Volker; Zrenner, Rita; Sonnewald, Uwe
- The Plant Journal, Vol. 14, Issue 2
Passive Entry of CO 2 and Its Energy-dependent Intracellular Conversion to HCO in Cyanobacteria Are Driven by a Photosystem I-generated ΔμH +
journal, April 2001
- Tchernov, Dan; Helman, Yael; Keren, Nir
- Journal of Biological Chemistry, Vol. 276, Issue 26
Alterations in Global Patterns of Gene Expression in Synechocystis sp. PCC 6803 in Response to Inorganic Carbon Limitation and the Inactivation of ndhR , a LysR Family Regulator
journal, November 2003
- Wang, Hong-Liang; Postier, Bradley L.; Burnap, Robert L.
- Journal of Biological Chemistry, Vol. 279, Issue 7
Increased fructose 1,6-bisphosphate aldolase in plastids enhances growth and photosynthesis of tobacco plants
journal, February 2012
- Uematsu, Kimio; Suzuki, Nobuaki; Iwamae, Tomoko
- Journal of Experimental Botany, Vol. 63, Issue 8
Cellular inorganic carbon fluxes in Trichodesmium: a combined approach using measurements and modelling
journal, November 2014
- Eichner, Meri; Thoms, Silke; Kranz, Sven A.
- Journal of Experimental Botany, Vol. 66, Issue 3
CyAbrB2 Contributes to the Transcriptional Regulation of Low CO 2 Acclimation in Synechocystis sp. PCC 6803
journal, September 2016
- Orf, Isabel; Schwarz, Doreen; Kaplan, Aaron
- Plant and Cell Physiology, Vol. 57, Issue 10
A Small Decrease in Rubisco Content by Individual Suppression of RBCS Genes Leads to Improvement of Photosynthesis and Greater Biomass Production in Rice Under Conditions of Elevated CO2
journal, February 2017
- Kanno, Keiichi; Suzuki, Yuji; Makino, Amane
- Plant and Cell Physiology, Vol. 58, Issue 3
Sensing of Inorganic Carbon Limitation in Synechococcus PCC7942 Is Correlated with the Size of the Internal Inorganic Carbon Pool and Involves Oxygen
journal, November 2005
- Woodger, Fiona J.; Badger, Murray R.; Price, G. Dean
- Plant Physiology, Vol. 139, Issue 4
Combined Effects of CO 2 and Light on the N 2 -Fixing Cyanobacterium Trichodesmium IMS101: Physiological Responses
journal, July 2010
- Kranz, Sven A.; Levitan, Orly; Richter, Klaus-Uwe
- Plant Physiology, Vol. 154, Issue 1
Early Evolution of Photosynthesis
journal, October 2010
- Blankenship, Robert E.
- Plant Physiology, Vol. 154, Issue 2
Advancing Our Understanding and Capacity to Engineer Nature’s CO 2 -Sequestering Enzyme, Rubisco
journal, October 2010
- Whitney, Spencer M.; Houtz, Robert L.; Alonso, Hernan
- Plant Physiology, Vol. 155, Issue 1
Photoreduction of O 2 Primes and Replaces CO 2 Assimilation
journal, September 1976
- Radmer, Richard J.; Kok, Bessel
- Plant Physiology, Vol. 58, Issue 3
Active Transport of CO 2 by the Cyanobacterium Synechococcus UTEX 625 : Measurement by Mass Spectrometry
journal, March 1988
- Miller, Anthony G.; Espie, George S.; Canvin, David T.
- Plant Physiology, Vol. 86, Issue 3
Simultaneous Transport of CO 2 and HCO 3 − by the Cyanobacterium Synechococcus UTEX 625
journal, July 1988
- Espie, George S.; Miller, Anthony G.; Birch, Douglas G.
- Plant Physiology, Vol. 87, Issue 3
Glycolaldehyde Inhibits CO 2 Fixation in the Cyanobacterium Synechococcus UTEX 625 without Inhibiting the Accumulation of Inorganic Carbon or the Associated Quenching of Chlorophyll a Fluorescence
journal, November 1989
- Miller, Anthony G.; Canvin, David T.
- Plant Physiology, Vol. 91, Issue 3
A Small Decrease of Plastid Transketolase Activity in Antisense Tobacco Transformants Has Dramatic Effects on Photosynthesis and Phenylpropanoid Metabolism
journal, March 2001
- Henkes, Stefan; Sonnewald, Uwe; Badur, Ralf
- The Plant Cell, Vol. 13, Issue 3
A thylakoid‐located carbonic anhydrase regulates CO 2 uptake in the cyanobacterium Synechocystis sp. PCC 6803
journal, December 2018
- Sun, Nan; Han, Xunling; Xu, Min
- New Phytologist, Vol. 222, Issue 1
Simultaneous stimulation of sedoheptulose 1,7-bisphosphatase, fructose 1,6-bisphophate aldolase and the photorespiratory glycine decarboxylase-H protein increases CO 2 assimilation, vegetative biomass and seed yield in Arabidopsis
journal, March 2017
- Simkin, Andrew J.; Lopez-Calcagno, Patricia E.; Davey, Philip A.
- Plant Biotechnology Journal, Vol. 15, Issue 7
Transcriptional Regulation of the CO2-Concentrating Mechanism in a Euryhaline, Coastal Marine Cyanobacterium, Synechococcus sp. Strain PCC 7002: Role of NdhR/CcmR
journal, February 2007
- Woodger, F. J.; Bryant, D. A.; Price, G. D.
- Journal of Bacteriology, Vol. 189, Issue 9
Catalytic exchange of 18 O from 13 C 18 O-labelled CO 2 by wild-type cells and ecaA , ecaB , and ccaA mutants of the cyanobacteria Synechococcus PCC7942 and Synechocystis PCC6803
journal, June 1998
- So, Anthony KC; Van Spall, Harriette GC; Coleman, John R.
- Canadian Journal of Botany, Vol. 76, Issue 6
The functioning of the CO 2 concentrating mechanism in several cyanobacterial strains: a review of general physiological characteristics, genes, proteins, and recent advances
journal, June 1998
- Price, G. Dean; Sültemeyer, Dieter; Klughammer, Barbara
- Canadian Journal of Botany, Vol. 76, Issue 6
CO 2 CONCENTRATING MECHANISMS IN PHOTOSYNTHETIC MICROORGANISMS
journal, June 1999
- Kaplan, Aaron; Reinhold, Leonora
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 50, Issue 1
Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories
journal, November 2015
- Luan, Guodong; Qi, Yunjing; Wang, Min
- Biotechnology for Biofuels, Vol. 8, Issue 1
Genetic engineering of the Calvin cycle toward enhanced photosynthetic CO2 fixation in microalgae
journal, October 2017
- Yang, Bo; Liu, Jin; Ma, Xiaonian
- Biotechnology for Biofuels, Vol. 10, Issue 1
Regulation of CO2 Concentrating Mechanism in Cyanobacteria
journal, January 2015
- Burnap, Robert; Hagemann, Martin; Kaplan, Aaron
- Life, Vol. 5, Issue 1
A modular system for the measurement of CO 2 and O 2 gas flux and photosynthetic electron transport in microalgae : Algal photosynthetic efficiency system
journal, December 2012
- Oakley, Clinton A.; Hopkinson, Brian M.; Schmidt, Gregory W.
- Limnology and Oceanography: Methods, Vol. 10, Issue 12
Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803
journal, January 2020
- Durall, Claudia; Lindberg, Pia; Yu, Jianping
- Biotechnology for Biofuels, Vol. 13, Issue 1
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